Measurement of vasorelaxant substance derived from endothelium and innervating nerve by biocascade system

科学研究費補助金研究成果報告書研究種目: 基盤研究(C)研究期間: 1998～1999課題番号: 10670083研究代表者: 安屋敷 和秀（滋賀医科大学・医学部・助教授）研究分担者: 岡村 富夫（滋賀医科大学・医学部・教授

Effects of calcium antagonists on vascular endothelial, smooth muscle and sympathetic nerve functions

科学研究費補助金研究成果報告書研究種目: 基盤研究(C)研究期間: 1996～1997課題番号: 08670107研究代表者: 岡村 富夫（滋賀医科大学・医学部・助教授）研究分担者: 安屋敷 和秀（滋賀医科大学・医学部・助手

Role of PPARα in Pathogenesis of Endothelial Dysfunction Associated with Insulin Resistance

科学研究費補助金研究成果報告書研究種目: 基盤研究(C)研究期間: 2005～2006課題番号: 17590219研究代表者: 篠崎 一哉（滋賀医科大学・医学部・助手）研究分担者: 岡村 富夫（滋賀医科大学・医学部・教授）研究分担者: 安屋敷 和秀（滋賀医科大学・医学部・助教授

Stimulation of the Sphenopalatine Ganglion Induces Reperfusion and Blood-Brain Barrier Protection in the Photothrombotic Stroke Model

The treatment of stroke remains a challenge. Animal studies showing that electrical stimulation of the sphenopalatine ganglion (SPG) exerts beneficial effects in the treatment of stroke have led to the initiation of clinical studies. However, the detailed effects of SPG stimulation on the injured brain are not known.The effect of acute SPG stimulation was studied by direct vascular imaging, fluorescent angiography and laser Doppler flowmetry in the sensory motor cortex of the anaesthetized rat. Focal cerebral ischemia was induced by the rose bengal (RB) photothrombosis method. In chronic experiments, SPG stimulation, starting 15 min or 24 h after photothrombosis, was given for 3 h per day on four consecutive days. Structural damage was assessed using histological and immunohistochemical methods. Cortical functions were assessed by quantitative analysis of epidural electro-corticographic (ECoG) activity continuously recorded in behaving animals.Stimulation induced intensity- and duration-dependent vasodilation and increased cerebral blood flow in both healthy and photothrombotic brains. In SPG-stimulated rats both blood brain-barrier (BBB) opening, pathological brain activity and lesion volume were attenuated compared to untreated stroke animals, with no apparent difference in the glial response surrounding the necrotic lesion.SPG-stimulation in rats induces vasodilation of cortical arterioles, partial reperfusion of the ischemic lesion, and normalization of brain functions with reduced BBB dysfunction and stroke volume. These findings support the potential therapeutic effect of SPG stimulation in focal cerebral ischemia even when applied 24 h after stroke onset and thus may extend the therapeutic window of currently administered stroke medications