Basic Research on the vascular renin-angiotensin system

科学研究費補助金研究成果報告書研究種目: 一般研究(C)研究期間: 1991～1992課題番号: 03807153研究代表者: 岡村 富夫（滋賀医科大学・医学部・助教授）研究分担者: 安屋敷 和秀（滋賀医科大学・医学部・助手

Measurement of vasorelaxant substance derived from endothelium and innervating nerve by biocascade system

科学研究費補助金研究成果報告書研究種目: 基盤研究(C)研究期間: 1998～1999課題番号: 10670083研究代表者: 安屋敷 和秀（滋賀医科大学・医学部・助教授）研究分担者: 岡村 富夫（滋賀医科大学・医学部・教授

Effects of calcium antagonists on vascular endothelial, smooth muscle and sympathetic nerve functions

科学研究費補助金研究成果報告書研究種目: 基盤研究(C)研究期間: 1996～1997課題番号: 08670107研究代表者: 岡村 富夫（滋賀医科大学・医学部・助教授）研究分担者: 安屋敷 和秀（滋賀医科大学・医学部・助手

Function of vascular endothelium and vasomotor nerve in atherosclerotic animals

科学研究費補助金研究成果報告書研究種目: 基盤研究(C)研究期間: 2000～2001課題番号: 12672213研究代表者: 安屋敷 和秀（滋賀医科大学・医学部・助教授）研究分担者: 岡村 富夫（滋賀医科大学・医学部・教授）研究分担者: 藤岡 秀行（滋賀医科大学・医学部・助手

Role of PPARα in Pathogenesis of Endothelial Dysfunction Associated with Insulin Resistance

科学研究費補助金研究成果報告書研究種目: 基盤研究(C)研究期間: 2005～2006課題番号: 17590219研究代表者: 篠崎 一哉（滋賀医科大学・医学部・助手）研究分担者: 岡村 富夫（滋賀医科大学・医学部・教授）研究分担者: 安屋敷 和秀（滋賀医科大学・医学部・助教授

Analyses of projection route of nitroxidergic nerve and its transmission mechanism

科学研究費補助金研究成果報告書研究種目: 基盤研究(B)研究期間: 1999～2001課題番号: 11470023研究代表者: 岡村 富夫（滋賀医科大学・医学部・教授）研究分担者: 安屋敷 和秀（滋賀医科大学・医学部・助教授）研究分担者: 藤岡 秀行（滋賀医科大学・医学部・助手）研究協力者: 戸田 昇（滋賀医科大学・名誉教授

Analysis of Mechanisms Underlying Vascular Nitroxidergic Innervation and its Pathogenic Implication

科学研究費補助金研究成果報告書研究種目: 基盤研究(B)研究期間: 1996～1997課題番号: 08457028研究代表者: 戸田 昇（滋賀医科大学・医学部・教授）研究分担者: 岡村 富夫（滋賀医科大学・医学部・助教授）研究分担者: 安屋敷 和秀（滋賀医科大学・医学部・助手）研究分担者: 吉田 一秀（滋賀医科大学・医学部・助手

Stimulation of the Sphenopalatine Ganglion Induces Reperfusion and Blood-Brain Barrier Protection in the Photothrombotic Stroke Model

The treatment of stroke remains a challenge. Animal studies showing that electrical stimulation of the sphenopalatine ganglion (SPG) exerts beneficial effects in the treatment of stroke have led to the initiation of clinical studies. However, the detailed effects of SPG stimulation on the injured brain are not known.The effect of acute SPG stimulation was studied by direct vascular imaging, fluorescent angiography and laser Doppler flowmetry in the sensory motor cortex of the anaesthetized rat. Focal cerebral ischemia was induced by the rose bengal (RB) photothrombosis method. In chronic experiments, SPG stimulation, starting 15 min or 24 h after photothrombosis, was given for 3 h per day on four consecutive days. Structural damage was assessed using histological and immunohistochemical methods. Cortical functions were assessed by quantitative analysis of epidural electro-corticographic (ECoG) activity continuously recorded in behaving animals.Stimulation induced intensity- and duration-dependent vasodilation and increased cerebral blood flow in both healthy and photothrombotic brains. In SPG-stimulated rats both blood brain-barrier (BBB) opening, pathological brain activity and lesion volume were attenuated compared to untreated stroke animals, with no apparent difference in the glial response surrounding the necrotic lesion.SPG-stimulation in rats induces vasodilation of cortical arterioles, partial reperfusion of the ischemic lesion, and normalization of brain functions with reduced BBB dysfunction and stroke volume. These findings support the potential therapeutic effect of SPG stimulation in focal cerebral ischemia even when applied 24 h after stroke onset and thus may extend the therapeutic window of currently administered stroke medications

European Society for Sexual Medicine Consensus Statement on the Use of the Cavernous Nerve Injury Rodent Model to Study Postradical Prostatectomy Erectile Dysfunction

Introduction: Rodent animal models are currently the most used in vivo model in translational studies looking into the pathophysiology of erectile dysfunction after nerve-sparing radical prostatectomy. Aim: This European Society for Sexual Medicine (ESSM) statement aims to guide scientists toward utilization of the rodent model in an appropriate, timely, and proficient fashion. Methods: MEDLINE and EMBASE databases were searched for basic science studies, using a rodent animal model, looking into the consequence of pelvic nerve injury on erectile function. Main outcome measures: The authors present a consensus on how to best perform experiments with this rodent model, the details of the technique, and highlight possible pitfalls. Results: Owing to the specific issue—basic science—Oxford 2011 Levels of Evidence criteria cannot be applied. However, ESSM statements on this topic will be provided in which we summarize the ESSM position on various aspects of the model such as the use of the Animal Research Reporting In Vivo Experiments guideline and the of common range parameter for nerve stimulation. We also highlighted the translational limits of the model. Conclusion: The following statements were formulated as a suggestive guidance for scientists using the cavernous nerve injury model. With this, we hope to standardize and further improve the quality of research in this field. It must be noted that this model has its limitations