The INTEGRAL/SPI response and the Crab observations

The Crab region was observed several times by INTEGRAL for calibration purposes. This paper aims at underlining the systematic interactions between (i) observations of this reference source, (ii) in-flight calibration of the instrumental response and (iii) the development and validation of the analysis tools of the SPI spectrometer. It first describes the way the response is produced and how studies of the Crab spectrum lead to improvements and corrections in the initial response. Then, we present the tools which were developed to extract spectra from the SPI observation data and finally a Crab spectrum obtained with one of these methods, to show the agreement with previous experiments. We conclude with the work still ahead to understand residual uncertainties in the response.Comment: 4 pages, 4 figures, Proc. of the 5th INTEGRAL Workshop (Feb. 16-20 2004), to be published by ES

A linear CO chemistry parameterization in a chemistry-transport model: evaluation and application to data assimilation

This paper presents an evaluation of a new linear parameterization valid for the troposphere and the stratosphere, based on a first order approximation of the carbon monoxide (CO) continuity equation. This linear scheme (hereinafter noted LINCO) has been implemented in the 3-D Chemical Transport Model (CTM) MOCAGE (MOdèle de Chimie Atmospherique Grande Echelle). First, a one and a half years of LINCO simulation has been compared to output obtained from a detailed chemical scheme output. The mean differences between both schemes are about ±25 ppbv (part per billion by volume) or 15% in the troposphere and ±10 ppbv or 100% in the stratosphere. Second, LINCO has been compared to diverse observations from satellite instruments covering the troposphere (Measurements Of Pollution In The Troposphere: MOPITT) and the stratosphere (Microwave Limb Sounder: MLS) and also from aircraft (Measurements of ozone and water vapour by Airbus in-service aircraft: MOZAIC programme) mostly flying in the upper troposphere and lower stratosphere (UTLS). In the troposphere, the LINCO seasonal variations as well as the vertical and horizontal distributions are quite close to MOPITT CO observations. However, a bias of ~−40 ppbv is observed at 700 Pa between LINCO and MOPITT. In the stratosphere, MLS and LINCO present similar large-scale patterns, except over the poles where the CO concentration is underestimated by the model. In the UTLS, LINCO presents small biases less than 2% compared to independent MOZAIC profiles. Third, we assimilated MOPITT CO using a variational 3D-FGAT (First Guess at Appropriate Time) method in conjunction with MOCAGE for a long run of one and a half years. The data assimilation greatly improves the vertical CO distribution in the troposphere from 700 to 350 hPa compared to independent MOZAIC profiles. At 146 hPa, the assimilated CO distribution is also improved compared to MLS observations by reducing the bias up to a factor of 2 in the tropics. This study confirms that the linear scheme is able to simulate reasonably well the CO distribution in the troposphere and in the lower stratosphere. Therefore, the low computing cost of the linear scheme opens new perspectives to make free runs and CO data assimilation runs at high resolution and over periods of several years

INTEGRAL broadband spectroscopy of Vela X-1

The wind-accreting X-ray binary pulsar and cyclotron line source Vela X-1 has been observed extensively during INTEGRAL Core Program observations of the Vela region in June-July and November-December 2003. In the latter set of observations the source showed intense flaring -- see also Staubert et al. (2004), these proceedings. We present early results on time averaged and time resolved spectra, of both epochs of observations. A cyclotron line feature at ~53 keV is clearly detected in the INTEGRAL spectra and its broad shape is resolved in SPI spectra. The remaining issues in the calibration of the instruments do not allow to resolve the question of the disputed line feature at 20-25 keV. During the first main flare the average luminosity increases by a factor of \~10, but the spectral shape remains very similar, except for a moderate softening.Comment: Accepted for proceedings of 5th INTEGRAL Worksho

Spatial distribution and statistical properties of small-scale convective vortex-like motions in a quiet Sun region

High-resolution observations of a quiet Sun internetwork region taken with the Solar 1-m Swedish Telescope in La Palma are analyzed. We determine the location of small-scale vortex motions in the solar photospheric region by computing the horizontal proper motions of small-scale structures on time series of images. These plasma convectively-driven swirl motions are associated to: (1) downdrafts (that have been commonly explained as corresponding to sites where the plasma is cooled down and hence returned to the interior below the visible photospheric level), and (2) horizontal velocity vectors converging into a central point. The sink cores are proved to be the final destination of passive floats tracing plasma flows towards the center of each vortex. We establish the occurrence of these events to be 1.4 x 10^(-3) and 1.6 x 10^(-3) vortices Mm^(-2) min^(-1) respectively for two time series analyzed here.Comment: 8 pages, 6 figures. Accepted for publication in Monthly Notices of the Royal Astronomical Societ

EIS/Hinode observations of Doppler flow seen through the 40 arcsec wide slit

The Extreme ultraviolet Imaging Spectrometer (EIS) on board Hinode is the first solar telescope to obtain wide slit spectral images that can be used for detecting Doppler flows in transition region and coronal lines on the Sun and to relate them to their surrounding small scale dynamics. We select EIS lines covering the temperature range 6x10^4 K to 2x10^6 K that give spectrally pure images of the Sun with the 40 arcsec slit. In these images Doppler shifts are seen as horizontal brightenings. Inside the image it is difficult to distinguish shifts from horizontal structures but emission beyond the image edge can be unambiguously identified as a line shift in several lines separated from others on their blue or red side by more than the width of the spectrometer slit (40 pixels). In the blue wing of He II, we find a large number of events with properties (size and lifetime) similar to the well-studied explosive events seen in the ultraviolet spectral range. Comparison with X-Ray Telescope (XRT) images shows many Doppler shift events at the footpoints of small X-ray loops. The most spectacular event observed showed a strong blue shift in transition region and lower corona lines from a small X-ray spot that lasted less than 7 min. The emission appears to be near a cool coronal loop connecting an X-ray bright point to an adjacent region of quiet Sun. The width of the emission implies a line-of-sight velocity of 220 km/s. In addition, we show an example of an Fe XV shift with a velocity about 120 km/s, coming from what looks like a narrow loop leg connecting a small X-ray brightening to a larger region of X-ray emission.Comment: 12 pages, 8 figures, to be published in Solar Physic

An oligofluorene truxene based distributed feedback laser for biosensing applications

The first example of an all-organic oligofluorene truxene based distributed feedback laser for the detection of a specific protein–small molecule interaction is reported. The protein avidin was detected down to View the MathML source1μgmL−1 using our biotin-labelled biosensor platform. This interaction was both selective and reversible when biotin was replaced with desthiobiotin. Avidin detection was not perturbed by Bovine Serum Albumin up to View the MathML source50,000μgmL−1. Our biosensor offers a new detection platform that is both highly sensitive, modular and potentially re-usable

ZZ-boson polarization as a model-discrimination analyzer

Determining the spin of any new particle is critical in identifying the true theory among various extensions of the Standard Model (SM). The degree of $Z$-boson polarization in any two-body decay process $A\to B Z$ is sensitive to the spin assignments of two new particles $A$ and $B$. Considering all possible spin-0, 1/2 and 1 combinations in a renormalizable field theory, we demonstrate that $Z$-boson polarization can indeed play a role of a decisive and universal analyzer in distinguishing the different spin assignments.Comment: 10 pages, 3 figures, 1 tabl

Genetic determinants of gut microbiota composition and bile acid profiles in mice.

The microbial communities that inhabit the distal gut of humans and other mammals exhibit large inter-individual variation. While host genetics is a known factor that influences gut microbiota composition, the mechanisms underlying this variation remain largely unknown. Bile acids (BAs) are hormones that are produced by the host and chemically modified by gut bacteria. BAs serve as environmental cues and nutrients to microbes, but they can also have antibacterial effects. We hypothesized that host genetic variation in BA metabolism and homeostasis influence gut microbiota composition. To address this, we used the Diversity Outbred (DO) stock, a population of genetically distinct mice derived from eight founder strains. We characterized the fecal microbiota composition and plasma and cecal BA profiles from 400 DO mice maintained on a high-fat high-sucrose diet for ~22 weeks. Using quantitative trait locus (QTL) analysis, we identified several genomic regions associated with variations in both bacterial and BA profiles. Notably, we found overlapping QTL for Turicibacter sp. and plasma cholic acid, which mapped to a locus containing the gene for the ileal bile acid transporter, Slc10a2. Mediation analysis and subsequent follow-up validation experiments suggest that differences in Slc10a2 gene expression associated with the different strains influences levels of both traits and revealed novel interactions between Turicibacter and BAs. This work illustrates how systems genetics can be utilized to generate testable hypotheses and provide insight into host-microbe interactions

Novel insights into the cardio-protective effects of FGF21 in lean and obese rat hearts

Aims: Fibroblast growth factor 21 (FGF21) is a hepatic metabolic regulator with pleotropic actions. Its plasma concentrations are increased in obesity and diabetes; states associated with an increased incidence of cardiovascular disease. We therefore investigated the direct effect of FGF21 on cardio-protection in obese and lean hearts in response to ischemia. Methods and Results: FGF21, FGF21-receptor 1 (FGFR1) and beta-Klotho (βKlotho) were expressed in rodent, human hearts and primary rat cardiomyocytes. Cardiac FGF21 was expressed and secreted (real time RT-PCR/western blot and ELISA) in an autocrine-paracrine manner, in response to obesity and hypoxia, involving FGFR1-βKlotho components. Cardiac-FGF21 expression and secretion were increased in response to global ischemia. In contrast βKlotho was reduced in obese hearts. In isolated adult rat cardiomyocytes, FGF21 activated PI3K/Akt (phosphatidylinositol 3-kinase/Akt), ERK1/2(extracellular signal-regulated kinase) and AMPK (AMP-activated protein kinase) pathways. In Langendorff perfused rat [adult male wild-type wistar] hearts, FGF21 administration induced significant cardio-protection and restoration of function following global ischemia. Inhibition of PI3K/Akt, AMPK, ERK1/2 and ROR-α (retinoic-acid receptor alpha) pathway led to significant decrease of FGF21 induced cardio-protection and restoration of cardiac function in response to global ischemia. More importantly, this cardio-protective response induced by FGF21 was reduced in obesity, although the cardiac expression profiles and circulating FGF21 levels were increased. Conclusion: In an ex vivo Langendorff system, we show that FGF21 induced cardiac protection and restoration of cardiac function involving autocrine-paracrine pathways, with reduced effect in obesity. Collectively, our findings provide novel insights into FGF21-induced cardiac effects in obesity and ischemia

The phenotype of MEGF8-related Carpenter syndrome (CRPT2) is refined through the identification of eight new patients

Carpenter syndrome (CRPTS) is a rare autosomal recessive condition caused by biallelic variants in genes that encode negative regulators of hedgehog signalling (RAB23 [CRPT1] or, more rarely, MEGF8 [CRPT2]), and is characterised by craniosynostosis, polysyndactyly, and other congenital abnormalities. We describe a further six families comprising eight individuals with MEGF8-associated CRPT2, increasing the total number of reported cases to fifteen, and refine the phenotype of CRPT2 compared to CRPT1. The core features of craniosynostosis, polysyndactyly and (in males) cryptorchidism are almost universal in both CRPT1 and CRPT2. However, laterality defects are present in nearly half of those with MEGF8-associated CRPT2, but are rare in RAB23-associated CRPT1. Craniosynostosis in CRPT2 commonly involves a single midline suture in comparison to the multi-suture craniosynostosis characteristic of CRPT1. No patient to date has carried two MEGF8 gene alterations that are both predicted to lead to complete loss-of-function, suggesting that a variable degree of residual MEGF8 activity may be essential for viability and potentially contributing to variable phenotypic severity. These data refine the phenotypic spectrum of CRPT2 in comparison to CRPT1 and more than double the number of likely pathogenic MEGF8 variants in this rare disorder