Measuring and tracking vitamin B12: a review of current methods with a focus on optical spectroscopy

Published online: 13 Sep 2016Vitamin B12 deficiency has been associated with an increased risk of cognitive decline. This literature review explores the current methods available for measuring vitamin B12 in human blood, serum, and urine, and the need for a globally accepted reference range for vitamin B12. We present optical spectroscopy, including chemiluminescence measurements, absorption and fluorescence spectroscopy, surface plasmon resonance, and Raman spectroscopy, as a promising technique for detection and tracking of vitamin B12. Considerations for future research are highlighted, including enhancing the sensitivity of optical spectroscopy and prospective pathways to improve the reproducibility, selectivity, and speed of vitamin B12 detection.Georgios Tsiminis, Erik P. Schartner, Joanna L. Brooks, and Mark R. Hutchinso

Low-threshold organic laser based on an oligofluorene truxene with low optical losses

A blue-emitting distributed feedback laser based on a star-shaped oligofluorene truxene molecule is presented. The gain, loss, refractive index, and (lack of) anisotropy are measured by amplified spontaneous emission and variable-angle ellipsometry. The waveguide losses are very low for an organic semiconductor gain medium, particularly for a neat film. The results suggest that truxenes are promising for reducing loss, a key parameter in the operation of organic semiconductor lasers. Distributed feedback lasers fabricated from solution by spin-coating show a low lasing threshold of 270 W/cm(2) and broad tunability across 25 nm in the blue part of the spectrum

Novel imaging tools for investigating the role of immune signalling in the brain

Abstract not availableJonathan Henry W. Jacobsen, Lindsay M. Parker, Arun V. Everest-Dass, Erik P. Schartner, Georgios Tsiminis, Vasiliki Staikopoulos, Mark R. Hutchinson, Sanam Mustaf

Measurements of vitamin B12 in human blood serum using resonance Raman spectroscopy

Vitamin B12 (cobalamin and its derivatives) deficiency has been identified as a potential modifiable risk factor for dementia and Alzheimer’s disease. Chronic deficiency of vitamin B12 has been significantly associated with an increased risk of cognitive decline. An effective and efficient method for measuring vitamin B12 concentration in human blood would enable ongoing tracking and assessment of this potential modifiable risk factor. In this work we present an optical sensor based on resonance Raman spectroscopy for rapid measurements of vitamin B12 in human blood serum. The measurement takes less than a minute and requires minimum preparation (centrifuging) of the collected blood samples.G. Tsiminis, E. P. Schartner, J. L. Brooks, M. R. Hutchinso

Quantification of the fluorescence sensing performance of microstructured optical fibers compared to multi-mode fiber tips

Published 3 Aug 2016Microstructured optical fibers, particularly those with a suspended-core geometry, have frequently been argued as efficient evanescent-field fluorescence-based sensors. However, to date there has not been a systematic comparison between such fibers and the more common geometry of a multi-mode fiber tip sensor. In this paper we make a direct comparison between these two fiber sensor geometries both theoretically and experimentally. Our results confirm that suspended-core fibers provide a significant advantage in terms of total collected fluorescence signal compared to multi-mode fibers using an equivalent experimental configuration.Erik P. Schartner, Georgios Tsiminis, Matthew R. Henderson, Stephen C. Warren-Smith, and Tanya M. Monr

Extruded single ring hollow core optical fibers for Raman sensing

Abstract not availableG. Tsiminis, K. J. Rowland, H. Ebendorff-Heidepriem, N. A. Spooner and T. M. Monr

An oligofluorene truxene based distributed feedback laser for biosensing applications

The first example of an all-organic oligofluorene truxene based distributed feedback laser for the detection of a specific protein–small molecule interaction is reported. The protein avidin was detected down to View the MathML source1μgmL−1 using our biotin-labelled biosensor platform. This interaction was both selective and reversible when biotin was replaced with desthiobiotin. Avidin detection was not perturbed by Bovine Serum Albumin up to View the MathML source50,000μgmL−1. Our biosensor offers a new detection platform that is both highly sensitive, modular and potentially re-usable

A Probabilistic Approach to the Spatial Variability of Ground Properties in the Design of Urban Deep Excavation

Uncertainty in ground datasets often stems from spatial variability of soil parameters and changing groundwater regimes. In urban settings and where engineering ground interventions need to have minimum and well-anticipated ground movements, uncertainty in ground data leads to uncertain analysis, with substantial unwelcomed economical and safety implications. A probabilistic random set finite element modelling (RSFEM) approach is used to revisit the stability and serviceability of a 27 m deep submerged soil nailed excavation built into a cemented soil profile, using a variable water level and soil shear strength. Variation of a suite of index parameters, including mobilized working loads and moments in facing and soil inclusion elements, as well as stability and serviceability of facing and the integrated support system, are derived and contrasted with field monitoring data and deterministic FE modelling outputs. The validated model is then deployed to test the viability of using independent hydraulic actions as stochastic variables as an alternative to dependent hydraulic actions and soil shear strength. The achieved results suggest that utilizing cohesion as a stochastic variable alongside the water level predicts system uncertainty reasonably well for both actions and material response; substituting the hydraulic gradient produces a conservative probability range for the action response only

Impact of rare earth doping on the luminescence of lanthanum aluminum silicate glasses for radiation sensing

Large core soft glass fibers have been demonstrated to be promising candidates as intrinsic fiber sensors for radiation detection and dosimetry applications. Doping with rare earth ions enhanced their radiation sensitivity. SiO2-Al2O3-La2O3 (SAL) glasses offer easy fabrication of large core fibers with high rare earth concentration and higher mechanical strength than soft glasses. This paper evaluates the suitability of the SAL glass type for radiation dosimetry based on optically stimulated luminescence (OSL) via a comprehensive investigation of the spectroscopic and dosimetric properties of undoped and differently rare earth doped bulk SAL glass samples. Due to the low intensity of the rare earth luminescence peaks in the 250–400 nm OSL detection range, the OSL response for all the SAL glasses is not caused by the rare earth ions but by radiation-induced defects that act as intrinsic centers for the recombination of electrons and holes produced by the ionizing radiation, trapped in fabrication induced defect centers, and then released via stimulation with 470 nm light. The rare earth ions interfere with these processes involving intrinsic centers. This dosimetric behavior of highly rare earth doped SAL glasses suggests that enhancement of OSL response requires lower rare earth concentrations and/or longer wavelength OSL detection range