Determination of S alleles in Paviot × Levent apricot progenies by PCR and controlled pollination

In this study, the sexual incompatibility of Paviot and Levent apricot parents and 89 F1 (Paviot × Levent) progenies was determined by self-pollination experiments and S-allele-specific polymerase chain reaction (PCR) technique. According to the self-pollination and isolation analyses under field conditions, it was found that the Paviot genotype is self-compatible (SC), whereas the Levent genotype is self-incompatible (SI). It was determined that, of all the progenies, 55 had a fruit set below 5% and were self-incompatible, whereas 34 had a fruit set over 5% and were self-compatible. The PCR-based techniques showed that, in parallel to the data obtained from the field studies, 55 F1 progenies did not have Sc allele, whereas 34 progenies involved Sc allele. There were ScS2 alleles in the Paviot genotype and S20S52 alleles in the Levent genotype. It was determined that there were S2S20, S2S52, ScS20, and ScS52 alleles in 89 F1 progenies and the distribution of the four alleles in the progenies was found to be as follows: 35.9% S2S20, 25.8% S2S52, 23.6% ScS20, and 14.6% ScS52. F1 progenies nos. 41, 46, 86, and 89 should be used as pollinators in further breeding studies

Loss of genetic diversity as a signature of apricot domestication and diffusion into the Mediterranean Basin

Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699In order to reduce energy waste for artificial lights and subsequent air conditioning in plant growth chambers, the aim of this preliminary study was to evaluate the feasibility of growing the microvine under 100% of LED illumination. Plant growth under two different LED lights was compared amongst each other and with plants maintained in greenhouse conditions. Regarding the impact on the reproductive and vegetative systems, the study showed that LED light is suitable to grow microvines in confined environments. Plants exposed to LED light exhibited similar leaf emergence rate but reduced vegetative and reproductive organ size compared to plants grown in the greenhouse. Photosynthesis for plants exposed to LED light was higher than what is usually observed on grapevine under natural conditions

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P < 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely

Cevizlerde (Juglans regia L. ) farklı zaman ve aşı yöntemleri ile yapılan aşılamalarda değişik aşı bağlarına aşı başarılarına etkileri üzerine araştırmalar

Bu tezin, veri tabanı üzerinden yayınlanma izni bulunmamaktadır. Yayınlanma izni olmayan tezlerin basılı kopyalarına Üniversite kütüphaneniz aracılığıyla (TÜBESS üzerinden) erişebilirsiniz.-ABSTRAKT- Bu çalışma Van Meyve Üretme İstasyonunda, farklı aşılama zamanlarında, farklı göz aşısı yöntemlerinin değişik aşı bağları ile bağlanarak, cevizlerde aşı başarı oranlarını ortaya koymak amacıyla yapılmıştır. Araştırma sonucunda, en başarılı aşı-ABSTRAKT- Bu çalışma Van Meyve Üretme İstasyonunda, farklı aşılama zamanlarında, farklı göz aşısı yöntemlerinin değişik aşı bağları ile bağlanarak, cevizlerde aşı başarı oranlarını ortaya koymak amacıyla yapılmıştır. Araştırma sonucunda, en başarılı aş

Inheritance of S-genotypes in Paviot x Kabaasi apricot F-1 progenies

WOS: 000384537200009Self-incompatibility plays an important role in the fertilization of fruit species such as apricot. Apricot (Prunus armeniaca L.) shows gametophytic self-incompatibility, which is controlled by a multi-allelic S-locus. In this study, S-alleles of 77 F-1 progenies derived from Paviot, which is one of the French local cultivars, and Kabaasi, one of the most important Turkish dried apricot cultivars, parents were identified by S-RNase intron regions polymerase chain reaction (PCR) amplification and DNA sequencing. The results from the S-allele PCR analysis revealed that the Paviot female parent had an ScS2 genotype and the Kabaasi male parent had S1S9 alleles. Forty-three of the F-1 progenies showed self-compatibility allele (S-c) by having either ScS9 or ScS1 alleles. Thirty-four of the F-1 progenies were self-incompatible by having either S2S1 or S2S9 alleles. The distributions of detected alleles in F-1 progenies were determined as follows: ScS1 31.2%, S1S2 27.3%, ScS9 24.7% and S2S9 16.8%. The results from the study are relevant for the data obtained in apricot breeding programmes in the selection of crossing combinations and in the establishment of commercial orchards.Council of Higher Education of TurkeyMinistry of National Education - TurkeyThis research was supported by The Council of Higher Education of Turkey

Türkiye'de Kayısı Islah Çalışmaları ve Islah Edilen Yeni Çeşitler

Kayısı, güzel renk, tat ve aroması, yüksek A vitamini ve diyet lifi içeriğiyle yaz mevsiminde sevilerek tüketilen meyvelerin başında gelir. İlkbahar geç donlarının neden olduğu kayıplar, farklı iklim koşullarına zayıf adaptasyon, sofralık kayısılarda raf ömrünün kısa olması, şarka ve monilya hastalığının yol açtığı zararlar bugün kayısı yetiştiriciliğinde karşılaşılan en önemli sorunlardır. Son yıllarda tüketici tercihlerinde yaşanan hızlı değişimlere birlikte renk, tat, aroma ve irilik bakımından farklı özelliklere sahip yeni kayısı çeşitlerine talep artmıştır. Kayısı ıslah çalışmalarının önemli bölümü kuzey yarımkürede yoğunlaşmış olup 74 çeşit ile ABD ve 70 çeşit ile Fransa en fazla kayısı ıslahı yapılan ülkeler olarak dikkat çekmektedir. Bu süreçte Türkiye’de ise sadece on bir kayısı çeşidi tescil ettirilmiştir. Bu çeşitlerden yedisi tanesi (Alata Yıldızı, Çağataybey, Çağrıbey, Dr. Kaşka, Şahinbey, Dilbay ve Eylül) melezleme ıslah yöntemiyle, diğerleri ise seleksiyon yoluyla geliştirilmiştir. Alkaya kayısı çeşidi hem sofralık hem de kurutmalık değerlendirmeye uygundur. Eylül ve Mihrialibey geç, diğer çeşitler ise erkenci ve orta mevsim olgunlaşma özelliğine sahiptir. Bu makalede, ülkemizde bugüne kadar yapılan kayısı ıslah çalışmaları gözden geçirilerek bu çalışmalardan elde edilen sonuçlar kısaca özetlenmiş, tescil edilerek üretime sunulan yeni kayısı çeşitlerinin önemli özelliklerine yer verilmiştir

Some selected physico-chemical characteristics of wild and cultivated blackberry fruits (Rubus fruticosus L.) from Turkey

In this study, some selected physico-chemical properties such as fruit weight, fruit length, fruit width, total soluble solids (TSS), titratable acidity, TSS/acidity ratio, pH, total phenolic content, antioxidant activity and free radical scavenging capacity of 9 cultivated and 16 selected wild blackberry (Rubus fruticosus L.) genotypes grown in Turkey were investigated. The total phenolic content, antioxidant activity and free radical-scavenging capacity of blackberry cultivars and genotypes were determined by using Folin-Ciocalteu, beta-carotene bleaching and DPPH radical assays. The results showed that, average fruit weight and fruit dimensions were higher in cultivated blackberries than wild materials. However, TSS, acidity and pH values were higher in wild materials. The total phenolic contents of blackberry cultivars and wild genotypes were in a range of 584 (cv. Bartin) to 788 (cv. Chester) mg/100 g and 610 (Genotypr R2) to 1455 mg/100 g (Genotype R16), expressed as gallic acid equivalents (GAE), on afresh weight basis. Antioxidant activity of cultivated and wild growing blackberry fruits was found between 72.15 (cv. Arapaho)-89.75% (cv. Bursa 3) and 59.85 (R1)-87.42% (R10), respectively. The antioxidant activity of standard BHA was 85.07%. Different cultivars grown in same location consistently showed differences in antioxidant capacity. The results of this study outlines that the blackberry fruits tested are good sources of natural antioxidants

Hereditary Angioedema-Presenting as Recurrent Abdominal Pain

Studying domesticated species and their wild relatives allows understanding of the mechanisms of population divergence and adaptation, and identifying valuable genetic resources. Apricot is an important fruit in the Northern hemisphere, where it is threatened by the Plum pox virus (PPV), causing the sharka disease. The histories of apricot domestication and of its resistance to sharka are however still poorly understood. We used 18 microsatellite markers to genotype a collection of 230 wild trees from Central Asia and 142 cultivated apricots as representatives of the worldwide cultivated apricot germplasm; we also performed experimental PPV inoculation tests. The genetic markers revealed highest levels of diversity in Central Asian and Chinese wild and cultivated apricots, confirming an origin in this region. In cultivated apricots, Chinese accessions were differentiated from more Western accessions, while cultivated apricots were differentiated from wild apricots. An approximate Bayesian approach indicated that apricots likely underwent two independent domestication events, with bottlenecks, from the same wild population. Central Asian native apricots exhibited genetic subdivision and high frequency of resistance to sharka. Altogether, our results contribute to the understanding of the domestication history of cultivated apricot and point to valuable genetic diversity in the extant genetic resources of wild apricots