93 research outputs found
Physicochemical and Stability Evaluation of Topical Niosomal Encapsulating Fosinopril/Îł-Cyclodextrin Complex for Ocular Delivery
Funding Information: Funding: This work was financially supported by the European Unionâs Eurostar Program under project No. PREVIN E11008 and by The Second Century Fund (C2F), Chulalongkorn University. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.This study aimed to develop a chemically stable niosomal eye drop containing fosinopril (FOS) for lowering intraocular pressure. The effects of cyclodextrin (CD), surfactant types and membrane stabilizer/charged inducers on physiochemical and chemical properties of niosome were evaluated. The pH value, average particle size, size distribution and zeta potentials were within the acceptable range. All niosomal formulations were shown to be slightly hypertonic with low viscosity. SpanÂź 60/dicetyl phosphate niosomes in the presence and absence of ÎłCD were selected as the optimum formulations according to their high %entrapment efficiency and negative zeta potential values as well as controlled release profile. According to ex vivo permeation study, the obtained lowest flux and apparent permeability coefficient values confirmed that FOS/ÎłCD complex was encapsulated within the inner aqueous core of niosome and could be able to protect FOS from its hydrolytic degradation. The in vitro cytotoxicity revealed that niosome entrapped FOS or FOS/ÎłCD formulations were moderate irritation to the eyes. Furthermore, FOS-loaded niosomal preparations exhibited good physical and chemical stabilities especially of those in the presence of ÎłCD, for at least three months under the storage condition of 2â8 °C.Peer reviewe
A mild alkali treated jute fibre controlling the hydration behaviour of greener cement paste
To reduce the antagonistic effect of jute fibre on the setting and hydration of jute reinforced cement, modified jute fibre reinforcement would be a unique approach. The present investigation deals with the effectiveness of mild alkali treated (0.5%) jute fibre on the setting and hydration behaviour of cement. Setting time measurement, hydration test and analytical characterizations of the hardened samples (viz., FTIR, XRD, DSC, TGA and free lime estimation) were used to evaluate the effect of alkali treated jute fibre. From the hydration test, the time (t) required to reach maximum temperature for the hydration of control cement sample is estimated to be 860â
min, whilst the time (t) is measured to be 1040â
min for the hydration of a raw jute reinforced cement sample. However, the time (t) is estimated to be 1020â
min for the hydration of an alkali treated jute reinforced cement sample. Additionally, from the analytical characterizations, it is determined that fibre-cement compatibility is increased and hydration delaying effect is minimized by using alkali treated jute fibre as fibre reinforcement. Based on the analyses, a model has been proposed to explain the setting and hydration behaviour of alkali treated jute fibre reinforced cement composite
Self-Assembled Lipoplexes of Short Interfering RNA (siRNA) Using Spermine-Based Fatty Acid Amide Guanidines: Effect on Gene Silencing Efficiency
Four guanidine derivatives of N4,N9-diacylated spermine have been designed, synthesized, and characterized. These guanidine-containing cationic lipids bound siRNA and formed nanoparticles. Two cationic lipids with C18 unsaturated chains, N1,N12-diamidino-N4,N9-dioleoylspermine and N1,N12-diamidino-N4-linoleoyl-N9-oleoylspermine, were more efficient in terms of GFP expression reduction compared to the other cationic lipids with shorter C12 (12:0) and very long C22 (22:1) chains. N1,N12-Diamidino-N4-linoleoyl-N9-oleoylspermine siRNA lipoplexes resulted in GFP reduction (26%) in the presence of serum, and cell viability (64%). These data are comparable to those obtained with TransIT TKO. Thus, cationic lipid guanidines based on N4,N9-diacylated spermines are good candidates for non-viral delivery of siRNA to HeLa cells using self-assembled lipoplexes
Development of slate fiber reinforced high density polyethylene composites for injection molding
During the last decade the use of fiber reinforced composite materials has consolidated as an attracting alternative to traditional materials due to an excellent balance between mechanical properties and lightweight. One drawback related to the use of inorganic fibers such as those derived from siliceous materials is the relative low compatibility with conventional organic polymer matrices. Surface treatments with coupling agents and the use of copolymers allow increasing fiber-matrix interactions which has a positive effect on overall properties of composites. In this research work we report the use of slate fiber treated with different coupling agents as reinforcement for high density polyethylene from sugarcane. A silane (propyltrimethoxy silane; PTMS) and a graft copolymer (polyethylene-graft-maleic anhydride; PE-g-MA) were used to improve fiber-matrix interactions on HOPE-slate fiber. The effect of the different compatibilizing systems and slate fiber content were evaluated by scanning electron microscopy (SEM), dynamic thermomechanical analysis (DTMA) as well as mechanical properties (tensile, flexural and impact). The results show that the use of silane coupling agents leads to higher fiber-matrix interactions which has a positive effect on overall mechanical properties. Interesting results are obtained for composites containing 30 wt.% slate fiber previously treated with propyltrimethoxy silane (PTMS) with an increase in tensile and flexural strength of about 16% and 18% respectively. (C) 2014 Elsevier Ltd. All rights reserved.Authors thank "Ministerio de Economia y Competitividad" ref: MAT2011-28468-C02-02 and "Conselleria d'Educacio, Cultura i Esport" - Generalitat Valenciana ref: GV/2014/008 for financial support.Carbonell VerdĂș, A.; GarcĂa GarcĂa, D.; JordĂĄ Vilaplana, A.; Samper Madrigal, MD.; Balart Gimeno, RA. (2015). Development of slate fiber reinforced high density polyethylene composites for injection molding. Composites Part B: Engineering. 69:460-466. https://doi.org/10.1016/j.compositesb.2014.10.026S4604666
Preparation of polycaprolactone nanoparticles via supercritical carbon dioxide extraction of emulsions
Polycaprolactone (PCL) nanoparticles were produced via supercritical fluid extraction of emulsions (SFEE) using supercritical carbon dioxide (scCO2). The efficiency of the scCO2 extraction was investigated and compared to that of solvent extraction at atmospheric pressure. The effects of process parameters including polymer concentration (0.6â10% w/w in acetone), surfactant concentration (0.07 and 0.14% w/w) and polymer-to-surfactant weight ratio (1:1â16:1 w/w) on the particle size and surface morphology were also investigated. Spherical PCL nanoparticles with mean particle sizes between 190 and 350 nm were obtained depending on the polymer concentration, which was the most important factor where increase in the particle size was directly related to total polymer content in the formulation. Nanoparticles produced were analysed using dynamic light scattering and scanning electron microscopy. The results indicated that SFEE can be applied for the preparation of PCL nanoparticles without agglomeration and in a comparatively short duration of only 1 h
Poloxamer-based thermoresponsive ketorolac tromethamine in situ gel preparations : design, characterisation, toxicity and transcorneal permeation studies
This study was aimed at preparing, characterising and evaluating in situ gel formulations based on a blend of two hydrophilic polymers i.e. poloxamer 407 (P407) and poloxamer 188 (P188) for a sustained ocular delivery of ketorolac tromethamine (KT). Drug-polymer interaction studies were performed using {DSC} and FT-IR. The gelation temperature (Tsol-gel), gelation time, rheological behaviour, mucoadhesive characteristics of these gels, transcorneal permeation and ocular irritation as well as toxicity was investigated. {DSC} and FT-IR studies revealed that there may be electrostatic interactions between the drug and the polymers used. {P188} modified the Tsol/gel of {P407} bringing it close to eye temperature (35°C) compared with the formulation containing {P407} alone. Moreover, gels that comprised {P407} and {P188} exhibited a pseudoplastic behaviour at different concentrations. Furthermore, mucoadhesion study using mucin discs showed that in situ gel formulations have good mucoadhesive characteristics upon increasing the concentration of P407. When comparing formulations {PP11} and PP12, the work of adhesion decreased significantly (P < 0.001) from 377.9 ± 7.79 mN.mm to 272.3 ± 6.11 mN.mm. In vitro release and ex vivo permeation experiments indicated that the in situ gels were able to prolong and control {KT} release as only 48 of the {KT} released within 12 h. In addition, the HET-CAM and {BCOP} tests confirmed the non-irritancy of {KT} loaded in situ gels, and HET-CAM test demonstrated the ability of ocular protection against strongly irritant substances. {MTT} assay on primary corneal epithelial cells revealed that in situ gel formulations loaded with {KT} showed reasonable and acceptable percent cell viability compared with control samples
Coir and Sisal Fibers as Fillers in the Production of Eucalyptus Medium Density Particleboards - MDP
A lutein ocular delivery system based on nanovesicles entrapped in an in situ forming gel
Assessment of Physical and Mechanical Properties of Cement Panel Influenced by Treated and Untreated Coconut Fiber Addition
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