Trypanosoma cruzi DNA replication includes the sequential recruitment of pre-replication and replication machineries close to nuclear periphery

In eukaryotes, many nuclear processes are spatially compartmentalized. Previously, we have shown that in Trypanosoma cruzi, an early-divergent eukaryote, DNA replication occurs at the nuclear periphery where chromosomes remain constrained during the S phase of the cell cycle. We followed Orc1/Cdc6, a pre-replication machinery component and the proliferating cell nuclear antigen (PCNA), a component of replication machinery, during the cell cycle of this protozoon. We found that, at the G(1) stage, TcOrc1/Cdc6 and TcPCNA are dispersed throughout the nuclear space. During the G(1)/S transition, TcOrc1/Cdc6 migrates to a region close to nuclear periphery. At the onset of S phase, TcPCNA is loaded onto the DNA and remains constrained close to nuclear periphery. Finally, in G(2), mitosis and cytokinesis, TcOrc1/Cdc6 and TcPCNA are dispersed throughout the nuclear space. Based on these findings, we propose that DNA replication in T. cruzi is accomplished by the organization of functional machineries in a spatial-temporal manner.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Universidade Federal de São Paulo, Parasitol Lab, Inst Butantan, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniv Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Meyer, BR-21941 Rio de Janeiro, BrazilUniversidade Federal de São Paulo, Parasitol Lab, Inst Butantan, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilWeb of Scienc

Effects of COVID-19 on auditory and vestibular function in an adult patient: a longitudinal case study

ABSTRACT COVID-19 is a viral infection that mainly affects the lower respiratory tract and with the affinity of the Spike protein to the Angiotensin 2 converter enzyme, inducing a prothrombotic state which may lead to compromising of the cardiovascular, central nervous, auditory, and vestibular systems. Viral infections knowingly affect vestibular and auditory systems. This study aimed to present the auditory and vestibular findings of a woman without otoneurological complaints, before the infection with SARS-CoV-2. This is a longitudinal case report, presenting the evolution of the otoneurological clinical picture accompanied by the results of a battery of tests. The results evidenced a unilateral, permanent, cochlear sensorineural hearing loss through altered otoacoustic emissions albeit with normal tonal thresholds, accompanied by peripheral vestibular hypofunction which worsened with time, without any other factor that could justify these findings. This study shows the importance of the complete protocol of tests performed over time for a better understanding of long-time implications of the SARS-CoV-2 infection

Beneficios de la terapia con caballos en niños con paralisis cerebral : revisión integrativa

Analisar, na literatura científica, os benefícios da equoterapia em crianças com paralisia cerebral. Trata-se de uma revisão integrativa da literatura, com buscas nas bases de dados SciELO, LILACS e PubMed. Utilizou-se os descritores em ciências da saúde (DeCS), sendo eles: terapia assistida por cavalos, paralisia cerebral, criança, nos idiomas português, inglês e espanhol. Foram selecionados artigos completos de 2005 a 2020. A amostra foi composta por 11 artigos completos, organizados e caracterizados, de acordo com autor, título, ano, país do estudo, delineamento e os benefícios da terapêutica. A equoterapia, possibilita a melhora significativa da função motora, assim como na qualidade de vida, ao promover o autocuidado, melhora na autoestima, além da interação social. Oestudo oferece importantes contribuições aos profissionais de diversas áreas do conhecimento, quanto aos benefícios daequoterapia, no desenvolvimento biopsicossocial da criança com paralisia cerebral.Toanalyze, in the scientific literature, the benefits of hippotherapy in children with cerebral palsy. Method: This is an integrative literature review, searching the SciELO, LILACS and PubMed databases. In health sciences (DeCS) were used: horse-assisted therapy, cerebral palsy, child, in Portuguese, English and Spanish. Complete articles from 2005 to 2020 were selected.The sample consisted of 11 complete articles, organized and characterized according to author, title, year, country of study, design and benefits of therapy. Equine therapy enables the significant improvement of motor function, as well as quality of life, by promoting self-care, improvement in self-esteem, and social interaction. The study offers important contributions to professionals from various areas of knowledge regarding the benefits of hippotherapy in the biopsychosocial development of children with cerebral palsy.Analizar, en la literatura científica, los beneficios de la hipoterapia en niños con parálisis cerebral.Esta es una revisión bibliográfica integradora que busca en las bases de datos SciELO, LILACS y PubMed. Se utilizaron los descriptores en ciencias de la salud (DeCS): terapia asistida por caballos, parálisis cerebral, infantil, en portugués, inglés y español. Se seleccionaron artículos completos de 2005 a 2020. La muestra consistió en 11 artículos completos, organizados y caracterizados según el autor, título, año, país de estudio, diseño y beneficios de la terapia. La terapia equina permite la mejora significativa de la función motora, así como la calidad de vida, al promover el autocuidado, la mejora de la autoestima y la interacción social.El estudio ofrece importantes contribuciones a profesionales de diversas áreas del conocimiento sobre los beneficios de la hipoterapia en el desarrollo biopsicosocial de niños con parálisis cerebral

COVID-19: como se proteger e conter a propagação no trabalho?

The pandemic caused by COVID-19 is a major health challenge and has required the work process to be considered in the design and implementation of strategies to cope with it. The reduction of risks inherent to work, through hygiene and biosafety protocols is a constitutional right. In this sense, this booklet aims to disseminate guidelines related to biosafety practices in the field of occupational health with the adequacy of the environment and conducts for the gradual resumption of face-to-face activities in a pandemic period. This material is the result of an extension project entitled "Good Biosafety Practices and Occupational Health in Education with the advent of COVID-19: how to protect and contain the spread?", Which has contributed to the dissemination of information aimed at the reduction of damage to workers' health and psychosocial impacts. Considering Collective Health as a multi and transdisciplinary area, it has contributed to the education sector through health education strategies, aiming at adapting the conduct at work involving the different segments of workers.A pandemia causada pela COVID-19 é um grande desafio para a saúde e tem exigido que o processo de trabalho seja considerado na elaboração e implantação de estratégias para o seu enfrentamento. A redução dos riscos inerentes ao trabalho, por meio de protocolos de higiene e biossegurança é um direito constitucional. Nesse sentido, esta cartilha tem como objetivo divulgar orientações relacionadas às práticas de biossegurança no campo da saúde do trabalhador com a adequação do ambiente e de condutas para a retomada gradual das atividades presenciais em período de pandemia. Este material é resultado de um projeto de extensão intitulado "Boas Práticas de Biossegurança e a Saúde do Trabalhador na Educação com o advento da COVID-19: como se proteger e conter a propagação?", que tem contribuído para a disseminação de informações que visem à diminuição de danos à saúde do trabalhador e de impactos psicossociais. Considerando a Saúde Coletiva como uma área multi e transdisciplinar, ela tem contribuído para o setor da educação por meio de estratégias de educação em saúde, visando adequações das condutas no trabalho envolvendo os diferentes segmentos de trabalhadores

Analysis of the association between lactotransferrin (LTF) gene polymorphism and dental caries

OBJECTIVE: The present study evaluated the association between lactotransferrin (LTF) gene polymorphism (exon 2, A/G, Lys/Arg) and dental caries. MATERIAL AND METHODS: A convenience sample of 110 individuals, 12 years old, was divided into: group 1, 48 individuals without caries experience (DMFT=0), and group 2, 62 subjects with caries experience (DMFT>;1). DNA was obtained from a mouthwash with 3% glucose solution, followed by a scrapping of the oral mucosa. After DNA purification, polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP) was performed to access the study polymorphism. The LTF A/G (Lys/Arg) polymorphism had been previously reported as located in exon 1. RESULTS: Allele 1 of the study polymorphism was associated with low DMFT index and showed a protective effect against caries experience (OR=0.16, IC=0.03-0.76, p=0.01). CONCLUSIONS: Lactotransferrin A/G (exon 2, Lys/Arg) polymorphism was associated with susceptibility to dental caries in 12-year-old students

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Trajetórias da Educomunicação nas Políticas Públicas e a Formação de seus Profissionais

Esta obra é composta com os trabalhos apresentados no primeiro subtema, TRAJETÓRIA – Educação para a Comunicação como Política pública, nas perspectivas da Educomunicação e da Mídia-Educação, do II Congresso Internacional de Comunicação e Educação. Os artigos pretendem propiciar trocas de informações e produzir reflexões com os leitores sobre os caminhos percorridos, e ainda a percorrer, tendo como meta a expansão e a legitimação das práticas educomunicativas e/ou mídia-educativas como política pública para o atendimento à formação de crianças, adolescentes, jovens e adultos, no Brasil e no mundo

American College of Rheumatology Provisional Criteria for Clinically Relevant Improvement in Children and Adolescents With Childhood-Onset Systemic Lupus Erythematosus

10.1002/acr.23834ARTHRITIS CARE & RESEARCH715579-59