Editorial: 3D Modelling of Mammalian Embryos and Organs

The main scope of this Special issue was to gain understanding on how tissues and organs are arranged into integrative hierarchical levels of complexity, from the molecular to the morphological organization. To understand the underlying complexity of the relationships among these levels during morphogenesis or in the adult we must learn how to resolve single-cell spatial relationships in the three-dimensional (3D) organisation of tissues, organs and even of the whole organisms.Fil: Garagna, Silvia. Universita Degli Studi Di Pavia; ItaliaFil: Cebral, Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Aréchaga, Juan. Universidad del País Vasco; EspañaFil: Zuccotti, Maurizio. Universita Degli Studi Di Pavia; Itali

Golpe blando : El proceso de desestabilización al gobierno de Fernando Lugo

La escasa literatura acerca de los golpes blandos, desarrollada en Sudamérica principalmente por la revolución bolivariana en Venezuela, pone en el centro de la escena al filósofo estadounidense de la Albert Einstein Institution, Gene Sharp, como autor principal de la matriz teórica que los sustentan. Asimismo, el escritor argelino Ahmed Bensaada afirma que las revoluciones de los colores, como se dieron en llamar a las revueltas acontecidas en antiguas repúblicas soviéticas a comienzos del siglo XXI ―Serbia (2000), Georgia (2003), Ucrania (2004) y Kirguistán (2005)―, “estuvieron lideradas por movimientos que agrupaban a jóvenes activistas locales, pro occidentales, formados, que luchaban por la democratización de sus países. Todos esos movimientos que consiguieron derrocar los regímenes autocráticos establecidos utilizaron los métodos de acción no violentos teorizados por el filósofo estadounidense Gene Sharp y aplicados por Robert Helvey".Facultad de Periodismo y Comunicación Socia

Histone deacetylase inhibitors induce invasion of human melanoma cells in vitro via differential regulation of N-cadherin expression and RhoA activity

Background: Histone deacetylase inhibitors (HDACi) exert multiple cytotoxic actions on cancer cells. Currently, different synthetic HDACi are in clinical use or clinical trials; nevertheless, since both pro-invasive and anti-invasive activities have been described, there is some controversy about the effect of HDACi on melanoma cells. Methods: Matrigel and Collagen invasion assays were performed to evaluate the effect of several HDACi (Butyrate, Trichostatin A, Valproic acid and Vorinostat) on two human melanoma cell line invasion (A375 and HT-144). The expression of N- and E-Cadherin and the activity of the RhoA GTPase were analyzed to elucidate the mechanisms involved in the HDACi activity. Results: HDACi showed a pro-invasive effect on melanoma cells in vitro. This effect was accompanied by an up-regulation of N-cadherin expression and an inhibition of RhoA activity. Moreover, the down-regulation of N-cadherin through blocking antibodies or siRNA abrogated the pro-invasive effect of the HDACi and, additionally, the inhibition of the Rho/ROCK pathway led to an increase of melanoma cell invasion similar to that observed with the HDACi treatments. Conclusion: These results suggest a role of N-cadherin and RhoA in HDACi induced invasion and call into question the suitability of some HDACi as antitumor agents for melanoma patients

Cloning and expression analysis of hemoglobin genes from maize (\u3ci\u3eZea mays\u3c/i\u3e ssp. \u3ci\u3emays\u3c/i\u3e) and teosinte (\u3ci\u3eZea mays\u3c/i\u3e ssp. \u3ci\u3eparviglumis\u3c/i\u3e)

With the exception of barley and rice, little is known about the existence of hemoglobins (Hbs) in cereals. This work reports the cloning and analysis of hb genes from maize (Zea mays ssp. mays) and teosinte (Zea mays ssp. parviglumis). Coding sequences of maize and teosinte hb genes (hbm and hbt, respectively) are highly similar to each other and are interrupted by three introns located at identical positions as other plant hb genes. Sequences of predicted Hbm and Hbt proteins are identical. The hydropathic profile of Hbm and Hbt is highly similar to that of rice Hb1, suggesting that Hbm, Hbt and Hb1 have the same tertiary structure and biochemical properties. Expression analysis showed that low levels of Hb transcripts, but considerable levels of Hb proteins exist in maize embryonic organs. No Hb transcripts and proteins were detected in teosinte embryonic organs. Low levels of Hb proteins, but no Hb transcripts, were detected in maize and teosinte vegetative organs. These observations suggest that the regulation of hb genes is different in maize and teosinte embryonic organs, and that the expression of hb genes is down- or up-regulated in maize and teosinte, respectively, from germination to vegetative growing

Cloning and expression analysis of hemoglobin genes from maize (\u3ci\u3eZea mays\u3c/i\u3e ssp. \u3ci\u3emays\u3c/i\u3e) and teosinte (\u3ci\u3eZea mays\u3c/i\u3e ssp. \u3ci\u3eparviglumis\u3c/i\u3e)

With the exception of barley and rice, little is known about the existence of hemoglobins (Hbs) in cereals. This work reports the cloning and analysis of hb genes from maize (Zea mays ssp. mays) and teosinte (Zea mays ssp. parviglumis). Coding sequences of maize and teosinte hb genes (hbm and hbt, respectively) are highly similar to each other and are interrupted by three introns located at identical positions as other plant hb genes. Sequences of predicted Hbm and Hbt proteins are identical. The hydropathic profile of Hbm and Hbt is highly similar to that of rice Hb1, suggesting that Hbm, Hbt and Hb1 have the same tertiary structure and biochemical properties. Expression analysis showed that low levels of Hb transcripts, but considerable levels of Hb proteins exist in maize embryonic organs. No Hb transcripts and proteins were detected in teosinte embryonic organs. Low levels of Hb proteins, but no Hb transcripts, were detected in maize and teosinte vegetative organs. These observations suggest that the regulation of hb genes is different in maize and teosinte embryonic organs, and that the expression of hb genes is down- or up-regulated in maize and teosinte, respectively, from germination to vegetative growing

IAA : Información y actualidad astronómica (24)

Sumario : Máseres en el espacio.-- Venus Express.-- El complejo cinturón de asteroides.-- DECONSTRUCCIÓN Y otros ENSAYOS : Rotación diferencial y oscilaciones estelares.-- ACTUALIDAD.-- ENTRE BASTIDORES .-- HISTORIAS DE ASTRONOMÍA: El astrónomo de vista prodigiosa.-- CIENCIA: PILARES E INCERTIDUMBRES : Ondas gravitatorias.-- ACTIVIDADES IAA.Esta revista se publica con la ayuda FCT-08-0130 del Programa Nacional de Fomento de la Cultura Científica y Tecnológica 2008.N

Publicaciones científicas profesionales en España: situación actual y parámetros de calidad

Se realiza una revisión del estado actual y de los problemas fundamentales que existen hoy día en las publicaciones científicas profesionales españolas, así como de las posibles soluciones a los mismos. Particular interés se dedica al tema del uso de un idioma científico internacional, a la correcta selección de manuscritos y al buen uso de los indicadores bibliométricos.Espainiako argitalpen zientifiko profesionalen egungo egoera eta dituzten arazo nagusiak aztertzendira artikulu honetan, eta era berean arazo horietarako litezkeen konponbideez hitz egiten da. Interes berezia eskaintzen zaie nazioarteko zientzia hizkuntza bakarraren erabilerari, eskuizkribuen hautaketa egokiarieta adierazle bibliometrikoen erabilera zuzenari.On réalise une révision de l'état actuel et des problèmes fondamentaux qui existent aujourd'hui dans les publications scientifiques professionnelles espagnoles, ainsi que leurs solutions possibles. On consacre un intérêt particulier au thème d'usage d'une langue scientifique internationale et efficace pour la sélection correcte de manuscrits et pour le bon usage des indicateurs bibliométriques.We herein present an evaluation of the present state of professional scientific publications in Spain, and propose solutions for some of the fundamental problems which this enterprise is facing. Particular emphasis is placed on the use of an effective international scientific language, on the importance of rigorous evaluation of papers to be published and on the correct use of bibliometric indicators

Doctoris D. Ioannis de Arechaga et Cassas ... Extemporaneae commentationes ad textus forte oblatos pro petitionibus cathedrarum

Primera obra impresa latina de un autor cubanoColofónSign.: *4, [calderón]2, A-N4, O2. -- La última p. en bl.[12], 107, [1] p. ; 4