463 research outputs found
Effects of Training and Development on Job Satisfaction
This thesis will focus on the effect training and development in the workplace on employees job satisfaction.
Research has stated that with the proper kinds of training and development employees will be more satisfied with their jobs overall. Researchers believe that the kinds of training and development given to employees will also improve the overall quality and productivity of the work environment.
With companies trying to move into the global job market training and development has come to the for front of all companies improvement programs. Most of the large companies CEO believe that training and development is the only way in which a company will survive in the business world of today.
The purpose of this study is to determine if training and development in three different manufacturing environments effects job satisfaction . The hypothesis of this study is with proper training and development employees will be more satisfied with their jobs.
Twenty-five participants from Globe and Donaldson and Nineteen from Hudson Valley Polymer were surveyed. Globe had twenty-three males and two females. Donaldson had twenty males and five females. Hudson Valley Polymer had twelve males and seven females. Each participants took a licket scale survey on training and development. The data was analysis by descriptive, inferential statistics and multivariates statistics.
Results of the analysis produced evidence that the employees from Globe, Hudson Valley Polymer and a combined Globe and Hudson Valley Polymer accepted the hypothesis. The employees for these two companies felt the company was doing an acceptable job on training and development and they were satisfied with their jobs. Donaldson on the other hand did not accept the hypothesis but they also did not accept the null hypothesis. Donaldson employees felt that the company was doing a poor job on training and development and they were not satisfied with their jobs
Reliability-based methodology for bridge inspection planning
Title from PDF of title page (University of Missouri--Columbia, viewed on June 8, 2012).The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.Thesis advisor: Dr. Glenn WasherIncludes bibliographical references.M.S. University of Missouri--Columbia 2011."December 2011"This research program was created with the goal of improving bridge safety and reliability while also improving the allocation of bridge inspection resources. The research reported herein was completed as a part of a larger project with the objective of developing a recommended bridge inspection practice for bridges within the United States. Traditionally, bridges in the United States are inspected at fixed time intervals of 24 months, with special programs in place to either extend or lessen this interval, based on certain conditions. This fixed inspection interval results in newer bridges, with little or no damage, being inspected with the same frequency as older, possibly more deteriorated bridges. This creates a situation where bridge inspection resources are allocated evenly across an inventory even though the inspection needs of certain bridges may be greater than others. Through this research program, a bridge inspection planning methodology has been developed which is based on reliability theory and incorporates the knowledge and expertise of bridge owners to more rationally determine bridge inspection needs. The methodology is based on the determination of the likelihood of failure for specific bridge components based on design, loading, and condition characteristics and the perceived consequence of failure, based on an owner's expertise and experience. By combining these expressions of likelihood and consequence for each component, a maximum inspection interval for the entire bridge can be determined through the use of risk matrices
Characterization of bovine rod outer segment G-protein
Journal ArticleA simple modified procedure is described for isolating and purifying peripherally bound membrane proteins from bovine rod outer segment disks. The methods yield milligram quantities of G-protein and cGMP phosphodiesterase which are suitable for reconstitution with membranes containing visual pigments. The properties of cGMP phosphodiesterase have been previously characterized (Baehr, W., Devlin, M. J. & Applebury, M. L. (1979) J. Biol. Chem 254, 11669-11677). Here we report the characterization and properties of the rod outer segment G-protein, a complex of three subunits a, B, an
Retinal degeneration is rescued in transgenic rd mice by expression of the cGMP phosphodiesterase ß subunit
The ß subunit of the cGMP phosphodiesterase (PDE) gene has been identified as the candidate gene for retinal degeneration in the rd mouse. To study the molecular mechanisms underlying degeneration and the potential for gene repair, we have expressed a functional bovine cGMP PDE ß subunit in transgenic rd mice. One transgenic mouse line showed complete photoreceptor rescue across the entire span of the retina. A second independently derived line showed partial rescue in which photoreceptors in the superior but not the inferior hemisphere of the retina were rescued. In the latter animals, intermediate stages of degeneration were observed in the transition zone between rescued and diseased photoreceptors. Pathologic changes in the retina ranged from vesiculation of the basalmost outer segment discs in otherwise structurally intact rod cells to photoreceptors with highly disorganized outer segments and intact inner segments. Totally or partially rescued retinas showed a corresponding restoration of cGMP PDE activity, whereas nonrescued retinas had minimal enzyme activity, characteristic of the rd phenotype. These transgenic animals provide models for studying the molecular basis of retinal degenerative disease and conclusively demonstrate that the phenotype of rd mice is produced by a defect in the ß subunit of cGMP PDE
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The spectral sensitivity of cone vision in the diurnal murid, Rhabdomys pumilio
An animal’s temporal niche – the time of day at which it is active – is known to drive a variety of adaptations in the visual system. This includes variations in the topography, spectral sensitivity and density of retinal photoreceptors, and changes in the eye’s gross anatomy and spectral transmission characteristics. We have characterised visual spectral sensitivity in the murid rodent Rhabdomys pumilio (‘the four-striped grass mouse’), which is the same family as (nocturnal) mice and rats, but exhibits a strong diurnal niche. As is common in diurnal species, the Rhabdomys lens acts as a long-pass spectral filter, providing limited transmission of light <400nm. Conversely, we found strong sequence homologies with the Rhabdomys SWS and MWS opsins and those of related nocturnal species (mice and rats) whose SWS opsins are maximally sensitive in the near UV. We continued to assess in vivo spectral sensitivity of cone vision using electroretinography and multi-channel recordings from the visual thalamus. These revealed that responses across the human visible range could be adequately described by those of a single pigment (assumed to be MWS opsin) maximally sensitive ~500nm, but that sensitivity in the near UV required inclusion of a second pigment whose peak sensitivity lay well into the UV range (λmax <400nm, likely ~360nm). We therefore conclude that, despite the UV-filtering effects of the lens, the Rhabdomys retains an SWS pigment with a UV-A λmax. In effect, this somewhat paradoxical combination of long-pass lens and UV-A λmax results in narrow-band sensitivity for SWS cone pathways in the UV-A range
Surface modification of Ti-6Al-4V alloy for biomineralization and specific biological response: Part II, Alkaline phosphatase grafting
Titanium and its alloys are the most widespread
materials for the realization of orthopaedic and dental implants due to their good mechanical properties and biocompatibility.
Surface functionalization of biomaterials
aimed to improve and quicken implant integration and tissue regeneration is an active research field. The opportunity
to confer biological activity (ability to directly stimulate cells with proper biological signals) to the Ti6Al4 V alloy, previously modified to be bioactive from
the inorganic point of view (apatite precipitation), was explored in this research work. The alkaline phosphatase
(ALP) enzyme was grafted to metal surface via tresyl chloride activation, maintaining its activity. A synergistic effect between biological functionalization and inorganic
bioactivity was observed
Standard Anatomical and Visual Space for the Mouse Retina: Computational Reconstruction and Transformation of Flattened Retinae with the Retistruct Package
The concept of topographic mapping is central to the understanding of the visual system at many levels, from the developmental to the computational. It is important to be able to relate different coordinate systems, e.g. maps of the visual field and maps of the retina. Retinal maps are frequently based on flat-mount preparations. These use dissection and relaxing cuts to render the quasi-spherical retina into a 2D preparation. The variable nature of relaxing cuts and associated tears limits quantitative cross-animal comparisons. We present an algorithm, "Retistruct," that reconstructs retinal flat-mounts by mapping them into a standard, spherical retinal space. This is achieved by: stitching the marked-up cuts of the flat-mount outline; dividing the stitched outline into a mesh whose vertices then are mapped onto a curtailed sphere; and finally moving the vertices so as to minimise a physically-inspired deformation energy function. Our validation studies indicate that the algorithm can estimate the position of a point on the intact adult retina to within 8° of arc (3.6% of nasotemporal axis). The coordinates in reconstructed retinae can be transformed to visuotopic coordinates. Retistruct is used to investigate the organisation of the adult mouse visual system. We orient the retina relative to the nictitating membrane and compare this to eye muscle insertions. To align the retinotopic and visuotopic coordinate systems in the mouse, we utilised the geometry of binocular vision. In standard retinal space, the composite decussation line for the uncrossed retinal projection is located 64° away from the retinal pole. Projecting anatomically defined uncrossed retinal projections into visual space gives binocular congruence if the optical axis of the mouse eye is oriented at 64° azimuth and 22° elevation, in concordance with previous results. Moreover, using these coordinates, the dorsoventral boundary for S-opsin expressing cones closely matches the horizontal meridian
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Death by color: differential cone loss in the aging mouse retina
Differential cell death is a common feature of aging and age-related disease. In the retina, 30% of rod photoreceptors are lost over life in humans and rodents. However, studies have failed to show age-related cell death in mouse cone photoreceptors, which is surprising because cone physiological function declines with age. Moreover in human, differential loss of short wavelength cone function is an aspect of age-related retinal disease. Here, cones are examined in young (3-month-old) and aged (12-month-old) C57 mice and also in complement factor H knock out mice (CFH-/-) that have been proposed as a murine model of age-related macular degeneration. In vivo imaging showed significant age-related reductions in outer retinal thickness in both groups over this period. Immunostaining for opsins revealed a specific significant decline of >20% for the medium/long (M/L)-wavelength cones but only in the periphery. S cones numbers were not significantly affected by age. This differential cell loss was backed up with quantitative real-time polymerase chain reaction for the 2 opsins, again showing S opsin was unaffected, but that M/L opsin was reduced particularly in CFH-/- mice. These results demonstrate aged cone loss, but surprisingly, in both genotypes, it is only significant in the peripheral ventral retina and focused on the M/L population and not S cones. We speculate that there may be fundamental differences in differential cone loss between human and mouse that may question the validity of mouse models of human outer retinal aging and pathology
Lentiviral gene transfer of RPE65 rescues survival and function of cones in a mouse model of Leber congenital amaurosis.
BACKGROUND: RPE65 is specifically expressed in the retinal pigment epithelium and is essential for the recycling of 11-cis-retinal, the chromophore of rod and cone opsins. In humans, mutations in RPE65 lead to Leber congenital amaurosis or early-onset retinal dystrophy, a severe form of retinitis pigmentosa. The proof of feasibility of gene therapy for RPE65 deficiency has already been established in a dog model of Leber congenital amaurosis, but rescue of the cone function, although crucial for human high-acuity vision, has never been strictly proven. In Rpe65 knockout mice, photoreceptors show a drastically reduced light sensitivity and are subject to degeneration, the cone photoreceptors being lost at early stages of the disease. In the present study, we address the question of whether application of a lentiviral vector expressing the Rpe65 mouse cDNA prevents cone degeneration and restores cone function in Rpe65 knockout mice. METHODS AND FINDINGS: Subretinal injection of the vector in Rpe65-deficient mice led to sustained expression of Rpe65 in the retinal pigment epithelium. Electroretinogram recordings showed that Rpe65 gene transfer restored retinal function to a near-normal pattern. We performed histological analyses using cone-specific markers and demonstrated that Rpe65 gene transfer completely prevented cone degeneration until at least four months, an age at which almost all cones have degenerated in the untreated Rpe65-deficient mouse. We established an algorithm that allows prediction of the cone-rescue area as a function of transgene expression, which should be a useful tool for future clinical trials. Finally, in mice deficient for both RPE65 and rod transducin, Rpe65 gene transfer restored cone function when applied at an early stage of the disease. CONCLUSIONS: By demonstrating that lentivirus-mediated Rpe65 gene transfer protects and restores the function of cones in the Rpe65(-/-) mouse, this study reinforces the therapeutic value of gene therapy for RPE65 deficiencies, suggests a cone-preserving treatment for the retina, and evaluates a potentially effective viral vector for this purpose
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