Mastcam-Z multispectral database from the Perseverance rover’s traverse in the Jezero crater floor, Mars (sols 0-380)

NASA’s Mars-2020 Perseverance rover spent its first year in Jezero crater studying the mafic lava flows of the Máaz formation and the ultramafic cumulates of the Séítah formation. Perseverance’s Mastcam-Z instrument, a pair of multispectral, stereoscopic zoom-lens cameras, provides broadband red/green/blue (RGB), narrowband visible to near-infrared color (VNIR, 440-1020 nm wavelength range). We compiled Mastcam-Z spectra from Perseverance’s exploration of the Jezero crater floor in the first 380 sols of its mission. Here, we provide a database of ~2400 representative spectra with extensive metadata, and the locations of the regions of interest (ROIs) from which the spectra were extracted. We also include “natural color” red, green, blue (RGB) images for context, “enhanced color images” derived by stretching narrowband images, and “decorrelation stretch” (DCS) images. This dataset can serve as a baseline to interpret future observations from Perseverance’s ongoing exploration of Jezero crater, Mars

An exergy-based multi-objective optimisation model for energy retrofit strategies in non-domestic buildings

While the building sector has a significant thermodynamic improvement potential, exergy analysis has been shown to provide new insight for the optimisation of building energy systems. This paper presents an exergy-based multi-objective optimisation tool that aims to assess the impact of a diverse range of retrofit measures with a focus on non-domestic buildings. EnergyPlus was used as a dynamic calculation engine for first law analysis, while a Python add-on was developed to link dynamic exergy analysis and a Genetic Algorithm optimisation process with the aforementioned software. Two UK archetype case studies (an office and a primary school) were used to test the feasibility of the proposed framework. Different measures combinations based on retrofitting the envelope insulation levels and the application of different HVAC configurations were assessed. The objective functions in this study are annual energy use, occupants' thermal comfort, and total building exergy destructions. A large range of optimal solutions was achieved highlighting the framework capabilities. The model achieved improvements of 53% in annual energy use, 51% of exergy destructions and 66% of thermal comfort for the school building, and 50%, 33%, and 80% for the office building. This approach can be extended by using exergoeconomic optimisation

Consensus guidelines for the use and interpretation of angiogenesis assays

The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference

MEG3 long noncoding RNA regulates the TGF-β pathway genes through formation of RNA-DNA triplex structures

Long noncoding RNAs (lncRNAs) regulate gene expression by association with chromatin, but how they target chromatin remains poorly understood. We have used chromatin RNA immunoprecipitation-coupled high-throughput sequencing to identify 276 lncRNAs enriched in repressive chromatin from breast cancer cells. Using one of the chromatin-interacting lncRNAs, MEG3, we explore the mechanisms by which lncRNAs target chromatin. Here we show that MEG3 and EZH2 share common target genes, including the TGF-β pathway genes. Genome-wide mapping of MEG3 binding sites reveals that MEG3 modulates the activity of TGF-β genes by binding to distal regulatory elements. MEG3 binding sites have GA-rich sequences, which guide MEG3 to the chromatin through RNA-DNA triplex formation. We have found that RNA-DNA triplex structures are widespread and are present over the MEG3 binding sites associated with the TGF-β pathway genes. Our findings suggest that RNA-DNA triplex formation could be a general characteristic of target gene recognition by the chromatin-interacting lncRNAs

Neptune Odyssey: A Flagship Concept for the Exploration of the Neptune–Triton System

The Neptune Odyssey mission concept is a Flagship-class orbiter and atmospheric probe to the Neptune-Triton system. This bold mission of exploration would orbit an ice-giant planet to study the planet, its rings, small satellites, space environment, and the planet-sized moon Triton. Triton is a captured dwarf planet from the Kuiper Belt, twin of Pluto, and likely ocean world. Odyssey addresses Neptune system-level science, with equal priorities placed on Neptune, its rings, moons, space environment, and Triton. Between Uranus and Neptune, the latter is unique in providing simultaneous access to both an ice giant and a Kuiper Belt dwarf planet. The spacecraft - in a class equivalent to the NASA/ESA/ASI Cassini spacecraft - would launch by 2031 on a Space Launch System or equivalent launch vehicle and utilize a Jupiter gravity assist for a 12 yr cruise to Neptune and a 4 yr prime orbital mission; alternatively a launch after 2031 would have a 16 yr direct-to-Neptune cruise phase. Our solution provides annual launch opportunities and allows for an easy upgrade to the shorter (12 yr) cruise. Odyssey would orbit Neptune retrograde (prograde with respect to Triton), using the moon's gravity to shape the orbital tour and allow coverage of Triton, Neptune, and the space environment. The atmospheric entry probe would descend in ~37 minutes to the 10 bar pressure level in Neptune's atmosphere just before Odyssey's orbit-insertion engine burn. Odyssey's mission would end by conducting a Cassini-like "Grand Finale,"passing inside the rings and ultimately taking a final great plunge into Neptune's atmosphere