Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained and restrained whole larvae. For direct control of the environment around the heart another approach utilizes the dissected larvae and removal of the internal organs in order to bathe the heart in desired compounds. The exposed heart also allows membrane potentials to be monitored which can give insight of the ionic currents generated by the myocytes and for electrical conduction along the heart tube. These approaches have various advantages and disadvantages for future experiments that are discussed. The larval heart preparation provides an additional model besides the Drosophila skeletal NMJ to investigate the role of intracellular calcium regulation on cellular function. Learning more about the underlying ionic currents that shape the action potentials in myocytes in various species, one can hope to get a handle on the known ionic dysfunctions associated to specific genes responsible for various diseases in mammals

“Well nobody wants to see a hairy fanny do they?”: exploring the everyday narratives of young women’s perceptions and practices around genital appearance and sexual identities

With the rising use of social media and in particular the visual elements of ‘selfies’ by young adults, recent research has explored the ways in which the images of the female body are scrutinised and stigmatised if non-conformity towards an idealised body image is apparent (Chrisler, 2011). This current trend of social media surveillance and self-regulation has led to recent moves towards young females removing their pubic hair with clinicians claiming that it is now unusual to examine a female under the age of 30 years who still has pubic hair (Braun, Tricklebank & Clarke, 2013; Riddell, Varto & Hodgson, 2010). These are ways in which the intimate appearances of genitalia become enveloped within everyday life. This qualitative research uses one-to-one, semi-structured interviews analysed using a discursive analysis with females aged 18 to 30 years old, with differing sexual orientations to explore their own perceptions and practices of this particular grooming regime. This research is still in progress at this time but initial findings suggest that participants predominantly cite the removal of pubic hair as a hygienic issue together with narratives of non-shaving resulting in a lessening of sexual activity (this can take the form of self-monitoring whereby not having shaved before a night out will stem sexual activity together with partners’ (either male or female) influencing shaving behaviours by refusing to have oral sex if the vaginal area is not fully shaved. All participants cite pornography and the media (television programmes such as Geordie Shore) as being instrumental in this trend

Proteomic Analysis of a Noninvasive Human Model of Acute Inflammation and Its Resolution: The Twenty-one Day Gingivitis Model

The 21-day experimental gingivitis model, an established noninvasive model of inflammation in response to increasing bacterial accumulation in humans, is designed to enable the study of both the induction and resolution of inflammation. Here, we have analyzed gingival crevicular fluid, an oral fluid comprising a serum transudate and tissue exudates, by LC−MS/MS using Fourier transform ion cyclotron resonance mass spectrometry and iTRAQ isobaric mass tags, to establish meta-proteomic profiles of inflammation-induced changes in proteins in healthy young volunteers. Across the course of experimentally induced gingivitis, we identified 16 bacterial and 186 human proteins. Although abundances of the bacterial proteins identified did not vary temporally, Fusobacterium outer membrane proteins were detected. Fusobacterium species have previously been associated with periodontal health or disease. The human proteins identified spanned a wide range of compartments (both extracellular and intracellular) and functions, including serum proteins, proteins displaying antibacterial properties, and proteins with functions associated with cellular transcription, DNA binding, the cytoskeleton, cell adhesion, and cilia. PolySNAP3 clustering software was used in a multilayered analytical approach. Clusters of proteins that associated with changes to the clinical parameters included neuronal and synapse associated proteins

The prevalence and incidence of mental ill-health in adults with autism and intellectual disabilities

The prevalence, and incidence, of mental ill-health in adults with intellectual disabilities and autism were compared with the whole population with intellectual disabilities, and with controls, matched individually for age, gender, ability-level, and Down syndrome. Although the adults with autism had a higher point prevalence of problem behaviours compared with the whole adult population with intellectual disabilities, compared with individually matched controls there was no difference in prevalence, or incidence of either problem behaviours or other mental ill-health. Adults with autism who had problem behaviours were less likely to recover over a two-year period than were their matched controls. Apparent differences in rates of mental ill-health are accounted for by factors other than autism, including Down syndrome and ability level

Neurodevelopmental multimorbidity and educational outcomes of Scottish schoolchildren : A population-based record linkage cohort study

Data Availability: All health data are owned by the Information Services Division of NHS National Services Scotland (https://www.isdscotland.org), and all education data are owned by the ScotXed Unit, which is part of the Educational Analytical Services Division within the Learning and Justice Directorate of the Scottish Government (www2.gov.scot/Topics/Statistics/ScotXed). Interested researchers may apply at these sites for data access. Funding: The study was sponsored by Health Data Research UK (www.hdruk.ac.uk) (grant reference number MR/S003800/1) (MF) which is a joint investment led by the Medical Research Council, together with the National Institute for Health Research (England), the Chief Scientist Office (Scotland), Health and Care Research Wales, Health and Social Care Research and Development Division (Public Health Agency, Northern Ireland), the Engineering and Physical Sciences Research Council, the Economic and Social Research Council, the British Heart Foundation and Wellcome. There was additional funding from the Carnegie Trust for the Universities of Scotland (grant reference VAC007974) (EES) and an MRC Mental Health Data Pathfinder grant (grant reference MC_PC_17217) (MF, JPP, DK, SC).Peer reviewedPublisher PD

Blazar 3C 454.3 in Outburst and Quiescence During 2005-2007: Two Variable Synchrotron Emission Peaks

We monitored the flaring blazar 3C 454.3 during 2005 June-July with the Spitzer Infrared Spectrograph (IRS: 15 epochs), Infrared Array Camera (IRAC: 12 epochs) and Multiband Imaging Photometer (MIPS: 2 epochs). We also made Spitzer IRS, IRAC, and MIPS observations from 2006 December-2007 January when the source was in a low state, the latter simultaneous with a single Chandra X-ray observation. In addition, we present optical and sub-mm monitoring data. The 2005-2007 period saw 3 major outbursts. We present evidence that the radio-optical SED actually consists of two variable synchrotron peaks, the primary at IR and the secondary at sub-mm wavelengths. The lag between the optical and sub-mm outbursts may indicate that these two peaks arise from two distinct regions along the jet separated by a distance of 0.07-5 pc. The flux at 5-35 microns varied by a factor of 40 and the IR peak varied in frequency from <1E13 Hz to 4E13 Hz between the highest and lowest states in 2005 and 2006, respectively. Variability was well correlated across the mid-IR band, with no measurable lag. Flares that doubled in flux occurred on a time scale of 3 days. The IR SED peak moved to higher frequency as a flare brightened, then returned to lower frequency as it decayed. The fractional variability amplitude increased with frequency, which we attribute to decreasing synchrotron-self absorption optical depth. Mid-IR flares may signal the re-energization of a shock that runs into inhomogeneities along the pre-existing jet or in the external medium. The synchrotron peak frequencies during each major outburst may depend upon both the distance from the jet apex and the physical conditions in the shocks. Variation of the Doppler parameter along a curved or helical jet is another possibility. Frequency variability of the IR synchrotron peak may have important consequences for the interpretation of the blazar sequence, and the presence of a secondary peak may give insight into jet structure.Comment: 38 pages, 15 figures, submitted to ApJS, comments welcom

DPP4 Truncated GM-CSF & IL-3 Manifest Distinct Receptor Binding & Regulatory Functions Compared to their Full Length Forms

Dipeptidylpeptidase 4 (DPP4/CD26) enzymatically cleaves select penultimate amino acids of proteins, including colony stimulating factors (CSFs), and has been implicated in cellular regulation. To better understand the role of DPP4 regulation of hematopoiesis, we analyzed the activity of DPP4 on the surface of immature blood cells and then comparatively assessed the interactions and functional effects of full-length (FL) and DPP4 truncated factors [(T)-GM-CSF and- IL-3] on both in vitro and in vivo models of normal and leukemic cells. T-GM-CSF and T-IL-3 had enhanced receptor binding, but decreased CSF activity, compared to their FL forms. Importantly, T-GM-CSF and T-IL-3 significantly, and reciprocally, blunted receptor binding and myeloid progenitor cell proliferation activity of both FL-GM-CSF and FL-IL-3 in vitro and in vivo. Similar effects were apparent in vitro using cluster forming cells from patients with Acute Myeloid Leukemia (AML) regardless of cytogenetic or molecular alterations and in vivo utilizing animal models of leukemia. This suggests that DPP4 T-molecules have modified binding and functions compared to their FL counterparts and may serve regulatory roles in normal and malignant hematopoiesis

Contemplating Synergistic Algorithms for the NASA ACE Mission

ACE is a proposed Tier 2 NASA Decadal Survey mission that will focus on clouds, aerosols, and precipitation as well as ocean ecosystems. The primary objective of the clouds component of this mission is to advance our ability to predict changes to the Earth's hydrological cycle and energy balance in response to climate forcings by generating observational constraints on future science questions, especially those associated with the effects of aerosol on clouds and precipitation. ACE will continue and extend the measurement heritage that began with the A-Train and that will continue through Earthcare. ACE planning efforts have identified several data streams that can contribute significantly to characterizing the properties of clouds and precipitation and the physical processes that force these properties. These include dual frequency Doppler radar, high spectral resolution lidar, polarimetric visible imagers, passive microwave and submillimeter wave radiometry. While all these data streams are technologically feasible, their total cost is substantial and likely prohibitive. It is, therefore, necessary to critically evaluate their contributions to the ACE science goals. We have begun developing algorithms to explore this trade space. Specifically, we will describe our early exploratory algorithms that take as input the set of potential ACE-like data streams and evaluate critically to what extent each data stream influences the error in a specific cloud quantity retrieval