21 research outputs found
Expression of the translation termination factor eRF1 is autoregulated by translational readthrough and 3'UTR intron-mediated NMD in Neurospora crassa
Eukaryotic release factor 1 (eRF1) is a translation termination factor that binds to the ribosome at stop codons. The expression of eRF1 is strictly controlled, since its concentration defines termination efficiency and frequency of translational readthrough. Here, we show that eRF1 expression in Neurospora crassa is controlled by an autoregulatory circuit that depends on the specific 3'UTR structure of erf1 mRNA. The stop codon context of erf1 promotes readthrough that protects the mRNA from its 3'UTR-induced nonsense-mediated mRNA decay (NMD). High eRF1 concentration leads to inefficient readthrough, thereby allowing NMD-mediated erf1 degradation. We propose that eRF1 expression is controlled by similar autoregulatory circuits in many fungi and seed plants and discuss the evolution of autoregulatory systems of different translation termination factors
The small G protein RAS2 is involved in the metabolic compensation of the circadian clock in the circadian model Neurospora crassa.
Accumulating evidence from both experimental and clinical investigations indicate a tight interaction between metabolism and circadian timekeeping; however, knowledge of the underlying mechanism is still incomplete. Metabolic compensation allows circadian oscillators to run with a constant speed at different substrate levels and therefore is a substantial criterion of a robust rhythm in a changing environment. Because previous data have suggested a central role of RAS2-mediated signaling in the adaptation of yeast to different nutritional environments, we examined the involvement of RAS2 in the metabolic regulation of the clock in the circadian model organism Neurospora crassa. We show that in a ras2-deficient strain, the period is longer than in the control. Moreover, unlike in wild type (wt), in Deltaras2 operation of the circadian clock was affected by glucose: compared with starvation conditions, the period was longer and the oscillation of expression of the frequency (frq) gene was dampened. In constant darkness the delayed phosphorylation of the FRQ protein and the long-lasting accumulation of FRQ in the nucleus were in accordance with the longer period and the less robust rhythm in the mutant. Whereas glucose did not affect the subcellular distribution of FRQ in wt, highly elevated FRQ levels were detected in the nucleus in Deltaras2. RAS2 interacted with the RAS-binding domain of the adenylate cyclase in vitro, and the cAMP analogue 8-Br-cAMP partially rescued the circadian phenotype in vivo. We propose therefore that RAS2 acts via a cAMP-dependent pathway and exerts significant metabolic control on the Neurospora circadian clock
Anaphylaxia oxiológiai vonatkozásai
Szakdolgozatom tĂ©mája az allergiás reakciĂł legsĂşlyosabb, akutan kialakulĂł formája az anaphylaxia, amelynek kialakulási esĂ©lye igen kicsi (1-3%), de fatális kimenetele miatt, fontos, hogy minden ellátĂł tisztában legyen ezzel a kĂłrkĂ©ppel Ă©s hatĂ©konyan elsajátĂtsa az azonnali ellátási elveket, valamint a prevenciĂłs lehetĹ‘sĂ©geket.BSc/BAápolás Ă©s betegellátás – mentĹ‘tisztmagyarnappaliV
HMW és LMW sikér alegységek meghatározása molekuláris markerekkel, európai országok őszi búza fajtáiban
HMW Ă©s LMW glutenin alegysĂ©gekre tervezett allĂ©l specifikus markerek optimalizálása Ă©s gyakoriságuknak a megállapĂtásaBSc/BABiolĂłgiag
A termĂ©szeti-, Ă©pĂtett Ă©s társadalmi környezetĂĽnk állapota, vĂ©delme Ă©s a fenntarthatĂłság Ă©rvĂ©nyesĂtĂ©se
Diplomadolgozatomban a termĂ©szeti-, Ă©pĂtett Ă©s társadalmi környezetĂĽnk állapotával, vĂ©delmĂ©vel Ă©s a fenntarthatĂłság Ă©rvĂ©nyesĂtĂ©sĂ©vel foglalkoztam. Saját kutatásom egy környezetvĂ©delmi Ă©s a fenntarthatĂłságra vonatkozĂł kĂ©rdĹ‘Ăv kitölttetĂ©se volt Ă©s ennek kiĂ©rtĂ©kelĂ©se.MSc/MAKörnyezettudományg