Two More Candidate AM Canum Venaticorum (AM CVn) Binaries from the Sloan Digital Sky Survey

AM CVn systems are a select group of ultracompact binaries with the shortest orbital periods of any known binary subclass; mass-transfer is likely from a low-mass (partially-)degenerate secondary onto a white dwarf primary, driven by gravitational radiation. In the past few years, the Sloan Digital Sky Survey (SDSS) has provided five new AM CVns. Here we report on two further candidates selected from more recent SDSS data. SDSS J1208+3550 is similar to the earlier SDSS discoveries, recognized as an AM CVn via its distinctive spectrum which is dominated by helium emission. From the expanded SDSS Data Release 6 (DR6) spectroscopic area, we provide an updated surface density estimate for such AM CVns of order 10^{-3.1} to 10^{-2.5} per deg^2 for 15<g<20.5. In addition, we present another new candidate AM CVn, SDSS J2047+0008, that was discovered in the course of followup of SDSS-II supernova candidates. It shows nova-like outbursts in multi-epoch imaging data; in contrast to the other SDSS AM CVn discoveries, its (outburst) spectrum is dominated by helium absorption lines, reminiscent of KL Dra and 2003aw. The variability selection of SDSS J2047+0008 from the 300 deg^2 of SDSS Stripe 82 presages further AM CVn discoveries in future deep, multicolor, and time-domain surveys such as LSST. The new additions bring the total SDSS yield to seven AM CVns thus far, a substantial contribution to this rare subclass, versus the dozen previously known.Comment: 19 pages, 5 figures, 1 table; submitted to A

A Live-Attenuated HSV-2 ICP0− Virus Elicits 10 to 100 Times Greater Protection against Genital Herpes than a Glycoprotein D Subunit Vaccine

Glycoprotein D (gD-2) is the entry receptor of herpes simplex virus 2 (HSV-2), and is the immunogen in the pharmaceutical industry's lead HSV-2 vaccine candidate. Efforts to prevent genital herpes using gD-2 subunit vaccines have been ongoing for 20 years at a cost in excess of $100 million. To date, gD-2 vaccines have yielded equivocal protection in clinical trials. Therefore, using a small animal model, we sought to determine if a live-attenuated HSV-2 ICP0− virus would elicit better protection against genital herpes than a gD-2 subunit vaccine. Mice immunized with gD-2 and a potent adjuvant (alum+monophosphoryl lipid A) produced high titers of gD-2 antibody. While gD-2-immunized mice possessed significant resistance to HSV-2, only 3 of 45 gD-2-immunized mice survived an overwhelming challenge of the vagina or eyes with wild-type HSV-2 (MS strain). In contrast, 114 of 115 mice immunized with a live HSV-2 ICP0− virus, 0ΔNLS, survived the same HSV-2 MS challenges. Likewise, 0ΔNLS-immunized mice shed an average 125-fold less HSV-2 MS challenge virus per vagina relative to gD-2-immunized mice. In vivo imaging demonstrated that a luciferase-expressing HSV-2 challenge virus failed to establish a detectable infection in 0ΔNLS-immunized mice, whereas the same virus readily infected naïve and gD-2-immunized mice. Collectively, these results suggest that a HSV-2 vaccine might be more likely to prevent genital herpes if it contained a live-attenuated HSV-2 virus rather than a single HSV-2 protein

Biophysical and electrochemical studies of protein-nucleic acid interactions

This review is devoted to biophysical and electrochemical methods used for studying protein-nucleic acid (NA) interactions. The importance of NA structure and protein-NA recognition for essential cellular processes, such as replication or transcription, is discussed to provide background for description of a range of biophysical chemistry methods that are applied to study a wide scope of protein-DNA and protein-RNA complexes. These techniques employ different detection principles with specific advantages and limitations and are often combined as mutually complementary approaches to provide a complete description of the interactions. Electrochemical methods have proven to be of great utility in such studies because they provide sensitive measurements and can be combined with other approaches that facilitate the protein-NA interactions. Recent applications of electrochemical methods in studies of protein-NA interactions are discussed in detail

Phloem sap and leaf δ13C, carbohydrates, and amino acid concentrations in Eucalyptus globulus change systematically according to flooding and water deficit treatment

Phloem is a central conduit for the distribution of photoassimilate, nutrients, and signals among plant organs. A revised technique was used to collect phloem sap from small woody plants in order to assess changes in composition induced by water deficit and flooding. Bled phloem sap δ13C and sugar concentrations were compared to δ13C of bulk material, soluble carbon extracts, and the neutral sugar fraction from leaves. Amino acid composition and inorganic ions of the phloem sap was also analysed. Quantitative, systematic changes were detected in phloem sap composition and δ13C in response to altered water availability. Phloem sap δ13C was more sensitive to changes of water availability than the δ13C of bulk leaf, the soluble carbon fraction, and the neutral soluble fraction of leaves. Changes in water availability also resulted in significant changes in phloem sugar (sucrose and raffinose), inorganic nutrient (potassium), and amino acid (phenylalanine) concentrations with important implications for the maintenance of phloem function and biomass partitioning. The differences in carbohydrate and amino acid composition as well as the δ13C in the phloem, along with a new model system for phloem research, offer an improved understanding of the phloem-mediated signal, nutrient, and photoassimilate transduction in relation to water availability

Disturbance and the Dynamics of Coral Cover on the Great Barrier Reef (1995–2009)

Coral reef ecosystems worldwide are under pressure from chronic and acute stressors that threaten their continued existence. Most obvious among changes to reefs is loss of hard coral cover, but a precise multi-scale estimate of coral cover dynamics for the Great Barrier Reef (GBR) is currently lacking. Monitoring data collected annually from fixed sites at 47 reefs across 1300 km of the GBR indicate that overall regional coral cover was stable (averaging 29% and ranging from 23% to 33% cover across years) with no net decline between 1995 and 2009. Subregional trends (10–100 km) in hard coral were diverse with some being very dynamic and others changing little. Coral cover increased in six subregions and decreased in seven subregions. Persistent decline of corals occurred in one subregion for hard coral and Acroporidae and in four subregions in non-Acroporidae families. Change in Acroporidae accounted for 68% of change in hard coral. Crown-of-thorns starfish (Acanthaster planci) outbreaks and storm damage were responsible for more coral loss during this period than either bleaching or disease despite two mass bleaching events and an increase in the incidence of coral disease. While the limited data for the GBR prior to the 1980's suggests that coral cover was higher than in our survey, we found no evidence of consistent, system-wide decline in coral cover since 1995. Instead, fluctuations in coral cover at subregional scales (10–100 km), driven mostly by changes in fast-growing Acroporidae, occurred as a result of localized disturbance events and subsequent recovery

Changes in the Number of Rural Bank Branches in India, 1991 to 2008

1. Underwater visual census (UVC) using SCUBA is a commonly used method for assessing reef fish communities. Evidence suggests, however, that fish avoid divers due to the sound of bubbles produced by open-circuit SCUBA, and avoidance behaviour is more pronounced as fishing pressure increases. Despite the potential for producing biased counts and conclusions, these behavioural effects have rarely been quantified, especially when assessing the effectiveness of marine protected areas (MPAs). 2. To test the magnitude of avoidance behaviour, we surveyed fish populations inside and outside two MPAs in Guam, using two diving systems: standard open-circuit (OC) SCUBA and a closed-circuit rebreather (CCR) that produces no bubbles. Data were collected using a diver-operated stereo-video system (stereo-DOV), which provided counts of relative abundance, measures of fish length and the minimum approach distance of the diver to a fish. 3. Inside MPAs, fish surveys conducted with CCR recorded similar community metrics to fish surveys conducted with conventional OC SCUBA. In contrast, outside the MPAs, the bubble-free diving system recorded 48% more species and up to 260% greater fish abundance. These differences reflected the ability of a diver wearing the silent CCR unit to sample the larger, most heavily targeted species that are shy of divers in fished areas. This difference was also large enough to change some results from ‘reject’ to ‘accept’ the null hypothesis of ‘no significant differences exist between fished and protected areas’. 4. The use of CCR for fish surveys clearly minimizes behavioural biases associated with fish avoiding open-circuit SCUBA divers. We recommend the use of this bubble-free diving system for surveys assessing reef fish populations, especially in areas where fish are heavily targeted by spearfishing. If fish behaviour is not accounted for, surveys using SCUBA could result in erroneous conclusions when comparing fished and protected areas. While the behaviour of fish towards divers is rarely mentioned in conclusions from studies using UVC, it is an important source of bias that should be acknowledged and minimized where possible

Data used in publication

Excel file with the data used for analysi