Seasonal isothiocyanates variation and market availability of Brassicaceae species consumed in Mendoza

Along with the recommendation of a healthy diet, it is suggested to increase the consumption of fruits and vegetables. Among the latter, Brassicaceae species are preferred, because they show many phytochemicals mainly belonging to the isothiocyanates (ITCs) family compounds, with proven activities related to the prevention of chronic diseases and cancer. A survey about seasonal availability and phytochemical levels of Brassicaceae species in the total of vegetables marketed in the province of Mendoza (located in the centre west of Argentina) was done. Results throw that Brassicaceae vegetables are an important part of Mendoza vegetable market reaching up to 23% of the vegetables commercialized. Regarding ITCs content, watercress and rocket were the vegetables with the highest ITCs levels, being, therefore, the most promising vegetables studied herein by their potential functional activities. Finally, high levels of variation (up to 10 times) on ITCs content along the year in a single species were found. These facts should be considered when designing Brassicaceae species phytochemical characterization assays to achieve more reliable results. This work represents the first report of Brassicaceae availability and seasonal phytochemical variability in local conditions. Highlights High levels of variation (up to 10 times) on ITCs content along the year in a single Brassicaceae species were found. Brassicaceae vegetables are an important part of Mendoza vegetable market reaching up to 23% of the vegetables commercialized. Considering ITCs content, watercress and rocket were the vegetables with the highest ITCs levels.Along with the recommendation of a healthy diet, it is suggested to increase the consumption of fruits and vegetables. Among the latter, Brassicaceae species are preferred, because they show many phytochemicals mainly belonging to the isothiocyanates (ITCs) family compounds, with proven activities related to the prevention of chronic diseases and cancer. A survey about seasonal availability and phytochemical levels of Brassicaceae species in the total of vegetables marketed in the province of Mendoza (located in the centre west of Argentina) was done. Results throw that Brassicaceae vegetables are an important part of Mendoza vegetable market reaching up to 23% of the vegetables commercialized. Regarding ITCs content, watercress and rocket were the vegetables with the highest ITCs levels, being, therefore, the most promising vegetables studied herein by their potential functional activities. Finally, high levels of variation (up to 10 times) on ITCs content along the year in a single species were found. These facts should be considered when designing Brassicaceae species phytochemical characterization assays to achieve more reliable results. This work represents the first report of Brassicaceae availability and seasonal phytochemical variability in local conditions. Highlights High levels of variation (up to 10 times) on ITCs content along the year in a single Brassicaceae species were found. Brassicaceae vegetables are an important part of Mendoza vegetable market reaching up to 23% of the vegetables commercialized. Considering ITCs content, watercress and rocket were the vegetables with the highest ITCs levels

molecular and morphological identification of an uncommon centrolophid fish

AbstractThe use of both morphological and molecular methods has allowed a fast and reliable species assignment of a fish that local fishermen with over thirty years of experience had never seen before. The identified species, Schedophilus medusophagus, is rare along Italian coasts, and this is the first documented record in the Central Tyrrhenian Sea for over 35 years. Its abundance should be evaluated on a continuous basis, as it might reflect biological consequences of environmental and climatic change. The mitochondrial sequences obtained in this study constitute a useful molecular tag for future research and may contribute to the phylogenetic debate on the status of the genus Schedophilus, of which S. medusophagus is the type species. Based on the existing literature, these preliminary molecular data support the hypothesis that the genus is not monophyletic

Population structure and genetic diversity characterization of a sunflower association mapping population using SSR and SNP markers

BACKGROUND: Argentina has a long tradition of sunflower breeding, and its germplasm is a valuable genetic resource worldwide. However, knowledge of the genetic constitution and variability levels of the Argentinean germplasm is still scarce, rendering the global map of cultivated sunflower diversity incomplete. In this study, 42 microsatellite loci and 384 single nucleotide polymorphisms (SNPs) were used to characterize the first association mapping population used for quantitative trait loci mapping in sunflower, along with a selection of allied open-pollinated and composite populations from the germplasm bank of the National Institute of Agricultural Technology of Argentina. The ability of different kinds of markers to assess genetic diversity and population structure was also evaluated.Fil: Filippi, Carla Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aguirre, Natalia Cristina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rivas, Juan Gabriel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zubrzycki, Jeremías Enrique. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Puebla, Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Cordes, Diego. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Manfredi; ArgentinaFil: Moreno, Maria V.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Manfredi; ArgentinaFil: Fusari, Corina M.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Alvarez, Daniel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Manfredi; ArgentinaFil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lia, Verónica Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Hyperoxemia and excess oxygen use in early acute respiratory distress syndrome : Insights from the LUNG SAFE study

Publisher Copyright: © 2020 The Author(s). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.Background: Concerns exist regarding the prevalence and impact of unnecessary oxygen use in patients with acute respiratory distress syndrome (ARDS). We examined this issue in patients with ARDS enrolled in the Large observational study to UNderstand the Global impact of Severe Acute respiratory FailurE (LUNG SAFE) study. Methods: In this secondary analysis of the LUNG SAFE study, we wished to determine the prevalence and the outcomes associated with hyperoxemia on day 1, sustained hyperoxemia, and excessive oxygen use in patients with early ARDS. Patients who fulfilled criteria of ARDS on day 1 and day 2 of acute hypoxemic respiratory failure were categorized based on the presence of hyperoxemia (PaO2 > 100 mmHg) on day 1, sustained (i.e., present on day 1 and day 2) hyperoxemia, or excessive oxygen use (FIO2 ≥ 0.60 during hyperoxemia). Results: Of 2005 patients that met the inclusion criteria, 131 (6.5%) were hypoxemic (PaO2 < 55 mmHg), 607 (30%) had hyperoxemia on day 1, and 250 (12%) had sustained hyperoxemia. Excess FIO2 use occurred in 400 (66%) out of 607 patients with hyperoxemia. Excess FIO2 use decreased from day 1 to day 2 of ARDS, with most hyperoxemic patients on day 2 receiving relatively low FIO2. Multivariate analyses found no independent relationship between day 1 hyperoxemia, sustained hyperoxemia, or excess FIO2 use and adverse clinical outcomes. Mortality was 42% in patients with excess FIO2 use, compared to 39% in a propensity-matched sample of normoxemic (PaO2 55-100 mmHg) patients (P = 0.47). Conclusions: Hyperoxemia and excess oxygen use are both prevalent in early ARDS but are most often non-sustained. No relationship was found between hyperoxemia or excessive oxygen use and patient outcome in this cohort. Trial registration: LUNG-SAFE is registered with ClinicalTrials.gov, NCT02010073publishersversionPeer reviewe

Valuing Modularity as a Real Option

We provide a general valuation approach for capital budgeting decisions involving the modularization in the design of a system. Within the framework developed by Baldwin and Clark (Baldwin, C. Y., K. B. Clark. 2000. Design Rules: The Power of Modularity. MIT Press, Cambridge, MA), we implement a valuation approach using a numerical procedure based on the least-squares Monte Carlo method proposed by Longstaff and Schwartz (Longstaff, F. A., E. S. Schwartz. 2001. Valuing American options by simulation: A simple least-squares approach. Rev. Financial Stud. 14(1) 113–147). The approach is accurate, general, and flexible

Valuing modularity as a real option

We provide a general valuation approach for capital budgeting decisions involving the modularization of a system. Within the framework developed by Baldwin and Clark (2000), we implement an approach using a numerical procedure based on the Least Squares Monte Carlo method proposed by Longstaff and Schwartz (2001). The approach is accurate, general and flexible.Real options, Modularity, Least Squares Monte Carlo

Simplified analytical methodology for glucosinolate hydrolysis products: A miniaturized extraction technique and multivariate optimization

Miniaturized extraction techniques are one of the most significant advances in analytical chemistry today. Nowadays there is a growing tendency among food researchers to develop simpler and robust methodologies that allow the determination of multiple analytes in different samples. Based on this concept, the aim of this work was to develop an optimized and validated methodology for the determination of four isothiocyanates (ITCs) and one indole: allyl ITC, erucin, sulforaphane, phenyl ITC and indole-3-carbinol present in Brassicaceae vegetables. Experimental design and multivariate analysis were the statistical tools used during the process. The dispersive liquid-liquid microextraction (DLLME) technique developed in this work was successfully applied to the analysis of nine Brassicaceae species.Fil: Fusari Gómez, Cecilia Mara. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Ramirez, Daniela Andrea. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Camargo, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentin