70 research outputs found
Growth hormone 1 gene (GH1) polymorphisms as possible markers of the production potential of beef cattle using the Brazilian Canchim breed as a model
The growth hormone 1 gene (GH1) is a candidate gene for body weight and weight gain in cattle since it plays a fundamental role in growth regulation. We investigated the GH1 gene AluI and DdeI restriction enzyme polymorphisms, located 149 bp apart in the cattle genome, as possible markers of the production potential of Canchim crossbreed cattle, a 5/8 Charolais (Bos taurus) and 3/8 Nelore (Bos indicus) breed developed in Brazil, by evaluating the birth weight, weaning weight, yearling weight and plasma insulin-like growth factor-1 (IGF-1) concentration of 7 month to 10 months old Canchim calves (n = 204) of known genealogy and which had been genotyped for the AluI and DdeI markers. Our results showed significant effect (p < 0.05) between the homozygous DdeI+/DdeI+ polymorphism and the estimated breeding value for weaning weight (ESB-WW), while the AluI leucine homozygous (L/L) and leucine/valine (L/V) heterozygous polymorphisms showed no significant effect on the traits studied. The restriction sites of the two enzymes led to the formation of haplotypes which also exerted a significant effect (p < 0.05) on the ESB-WW, with the largest difference being 8.5 kg in favor of the homozygous L plus DdeI+/L plus DdeI+ genotype over the heterozygous L plus DdeI-/V plus DdeI+ genotype
Calliphorid fly (Diptera, Calliphoridae) attraction to different colored traps in the Tingua Biological Reserve, Rio de Janeiro, Brazil
CONSTRUÇÃO DA IDENTIDADE DOCENTE DE UM ESTUDANTE DE LICENCIATURA EM CIÊNCIAS BIOLÓGICAS EM CURSO A DISTÂNCIA: UM CASO DE HIBRIDISMO
Anaerobic biodigestion of manure from finishing pig supplemented with ractopamine over different periods
Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp. aurantifolii
Background: Citrus canker is a disease that has severe economic impact on the citrus industry worldwide. There are three types of canker, called A, B, and C. The three types have different phenotypes and affect different citrus species. The causative agent for type A is Xanthomonas citri subsp. citri, whose genome sequence was made available in 2002. Xanthomonas fuscans subsp. aurantifolii strain B causes canker B and Xanthomonas fuscans subsp. aurantifolii strain C causes canker C. Results: We have sequenced the genomes of strains B and C to draft status. We have compared their genomic content to X. citri subsp. citri and to other Xanthomonas genomes, with special emphasis on type III secreted effector repertoires. In addition to pthA, already known to be present in all three citrus canker strains, two additional effector genes, xopE3 and xopAI, are also present in all three strains and are both located on the same putative genomic island. These two effector genes, along with one other effector-like gene in the same region, are thus good candidates for being pathogenicity factors on citrus. Numerous gene content differences also exist between the three cankers strains, which can be correlated with their different virulence and host range. Particular attention was placed on the analysis of genes involved in biofilm formation and quorum sensing, type IV secretion, flagellum synthesis and motility, lipopolysacharide synthesis, and on the gene xacPNP, which codes for a natriuretic protein. Conclusion: We have uncovered numerous commonalities and differences in gene content between the genomes of the pathogenic agents causing citrus canker A, B, and C and other Xanthomonas genomes. Molecular genetics can now be employed to determine the role of these genes in plant-microbe interactions. The gained knowledge will be instrumental for improving citrus canker control.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Conselho Nacional de Desenvolvimento CientIfico e Tecnologico (CNPq)Coordenacao para Aperfeicoamento de Pessoal de Ensino Superior (CAPES)Fundo de Defesa da Citricultura (FUNDECITRUS
Tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance
EFEITOS DA POLÍTICA DE SALÁRIO MÍNIMO SOBRE O MERCADO DE TRABALHO METROPOLITANO: UMA ANÁLISE EMPÍRICA A PARTIR DE VETORES AUTORREGRESSIVOS (VAR) - (2003-2015)
RESUMO Com intuito de verificar os efeitos que a política de salário mínimo exerce sobre o mercado de trabalho metropolitano brasileiro, o presente estudo contribui ao verificar a sua inter-relação com variáveis como taxa de atividade, grau de informalidade e taxa de desemprego em quatro regiões metropolitanas (Recife, Porto Alegre, Belo Horizonte e São Paulo), separadamente, no período de janeiro de 2003 a março de 2015. Ao utilizar o método de autorregressão vetorial (VAR), os resultados mostram que a relação entre salário mínimo e taxa de atividade se deu com ingresso de mão de obra apenas em uma região estudada. Nas demais regiões observam-se tanto uma tendência de queda no grau de informalidade quanto um aumento na taxa de desemprego
Genomic structure and marker-derived gene networks for growth and meat quality traits of Brazilian Nelore beef cattle
Abstract\ud
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Background\ud
Nelore is the major beef cattle breed in Brazil with more than 130 million heads. Genome-wide association studies (GWAS) are often used to associate markers and genomic regions to growth and meat quality traits that can be used to assist selection programs. An alternative methodology to traditional GWAS that involves the construction of gene network interactions, derived from results of several GWAS is the AWM (Association Weight Matrices)/PCIT (Partial Correlation and Information Theory). With the aim of evaluating the genetic architecture of Brazilian Nelore cattle, we used high-density SNP genotyping data (~770,000 SNP) from 780 Nelore animals comprising 34 half-sibling families derived from highly disseminated and unrelated sires from across Brazil. The AWM/PCIT methodology was employed to evaluate the genes that participate in a series of eight phenotypes related to growth and meat quality obtained from this Nelore sample.\ud
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Results\ud
Our results indicate a lack of structuring between the individuals studied since principal component analyses were not able to differentiate families by its sires or by its ancestral lineages. The application of the AWM/PCIT methodology revealed a trio of transcription factors (comprising VDR, LHX9 and ZEB1) which in combination connected 66 genes through 359 edges and whose biological functions were inspected, some revealing to participate in biological growth processes in literature searches.\ud
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Conclusions\ud
The diversity of the Nelore sample studied is not high enough to differentiate among families neither by sires nor by using the available ancestral lineage information. The gene networks constructed from the AWM/PCIT methodology were a useful alternative in characterizing genes and gene networks that were allegedly influential in growth and meat quality traits in Nelore cattle.This study was conducted with funding from EMBRAPA (Macroprograma1,\ud
01/2005) and FAPESP (process number 2012/23638-8). GBM, LLC, LCAR and\ud
MMA were granted CNPq fellowships. We thank Sean McWilliam, Marina R. S.\ud
Fortes, Edilson Guimaraes, Robson Rodrigues Santiago, Roselito F. da Silva,\ud
Fernando F. Cardoso, Flavia Aline Bressani, Wilson Malago Jr., Avelardo U. C.\ud
Ferreira, Michel E. B. Yamaguishi and Fabio D. Vieira for the help and\ud
technical assistance. The authors would like to acknowledge the\ud
collaborative efforts among EMBRAPA, University of Sao Paulo and CSIRO
Avaliação dos resultados de intervenção após mudanças realizadas nos cuidados peri-operatórios em pacientes submetidos a operações abdominais eletivas
Reconstructing the Deep Population History of Central and South America
We report genome-wide ancient DNA from 49 individuals forming four parallel time transects in Belize, Brazil, the Central Andes, and the Southern Cone, each dating to at least 9,000 years ago. The common ancestral population radiated rapidly from just one of the two early branches that contributed to Native Americans today. We document two previously unappreciated streams of gene flow between North and South America. One affected the Central Andes by 4,200 years ago, while the other explains an affinity between the oldest North American genome associated with the Clovis culture and the oldest Central and South Americans from Chile, Brazil, and Belize. However, this was not the primary source for later South Americans, as the other ancient individuals derive from lineages without specific affinity to the Clovis-associated genome, suggesting a population replacement that began at least 9,000 years ago and was followed by substantial population continuity in multiple regions
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