Isopentenyl diphosphate:dimethylallyl diphosphate isomerase. An improved purification of the enzyme and isolation of the gene from Saccharomyces cerevisiae.

Journal ArticleIsopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase) is an enzyme in the isoprenoid biosynthetic pathway which catalyzes the interconversion of the primary five-carbon homoallylic and allylic diphosphate building blocks. We report a substantially improved procedure for purification of this enzyme from Saccharomyces cerevisiae. An amino-terminal sequence (35 amino acids) was obtained from a highly purified preparation of IPP isomerase. Oligonucleotide probes based on the protein sequence were used to isolate the structural gene encoding IPP isomerase from a yeast lambda library. The cloned gene encodes a 33,350-dalton polypeptide of 288 amino acids. A 3.5-kilobase EcoRI fragment containing the gene was subcloned into the yeast shuttle vector YRp17. Upon transformation with plasmids containing the insert, a 5-6-fold increase in IPP isomerase activity was seen in transformed cells relative to YRp17 controls, confirming the identity of the cloned gene. This is the first reported isolation of the gene for IPP isomerase

Field-Based Assessment of the Role of Porcine Cytomegalovirus in Respiratory Disease of Nursery Pigs

Porcine cytomegalovirus (PCMV) is an ubiquitous infectious agent in swine population throughout the world. Field and some experimental observations have suggested that PCMV plays an important role in causing or enhancing respiratory and/or reproductive disease of swine. However, no actual measure of this has been documented. As the first step in assessing the economic significance of PCMV infection for swine herds in the United States, a field-based case-control study was conducted to evaluate the potential role of the virus in respiratory disease of young swine. The data in this study, thus far, suggest that there may be an association between PCMV infection and increased risk of respiratory disease development in nursery pig populations and that, as was expected, PCMV infection is a common finding among nursery pigs. In an era in which multifactorial respiratory disease and associated decrease in production efficiency is such a large concern, it may be prudent to consider PCMV when developing and implementing strategies for production management and pig flow

Accessing Healthy Food: A sentinel mapping study of healthy food retailing in Scotland

This study on the availability of an affordable healthy food shopping basket was commissioned by the Food Standards Agency Scotland and undertaken between 2005 and 2007 by the Centre for the Study of Retailing in Scotland

Controlling the collimation and rotation of hydromagnetic disk winds

(Abriged) We present a comprehensive set of axisymmetric, time-dependent simulations of jets from Keplerian disks whose mass loading as a function of disk radius is systematically changed. For a reasonable model for the density structure and injection speed of the underlying accretion disk, mass loading is determined by the radial structure of the disk's magnetic field structure. We vary this structure by using four different magnetic field configurations, ranging from the "potential" configuration (Ouyed&Pudritz 1997), to the increasingly more steeply falling Blandford&Payne (1982) and Pelletier&Pudritz (1992) models, and ending with a quite steeply raked configuration that bears similarities to the Shu X-wind model. We find that the radial distribution of the mass load has a profound effect on both the rotational profile of the underlying jet as well as the degree of collimation of its outflow velocity and magnetic field lines. We show analytically, and confirm by our simulations, that the collimation of a jet depends on its radial current distribution, which in turn is prescribed by the mass load. Models with steeply descending mass loads have strong toroidal fields, and these collimate to cylinders (this includes the Ouyed-Pudritz and Blandford-Payne outflows). On the other hand, the more gradually descending mass load profiles (the PP92 and monopolar distributions) have weaker toroidal fields, and these result in wide-angle outflows with parabolic collimation. We also present detailed structural information about jets such as their radial profiles of jet density, toroidal magnetic field, and poloidal jet speed, as well as an analysis of the bulk energetics of our different simulations.Comment: 20 journal pages, including 9 figures. Submitted to MNRA

Two-flow magnetohydrodynamical jets around young stellar objects

We present the first-ever simulations of non-ideal magnetohydrodynamical (MHD) stellar winds coupled with disc-driven jets where the resistive and viscous accretion disc is self-consistently described. The transmagnetosonic, collimated MHD outflows are investigated numerically using the VAC code. Our simulations show that the inner outflow is accelerated from the central object hot corona thanks to both the thermal pressure and the Lorentz force. In our framework, the thermal acceleration is sustained by the heating produced by the dissipated magnetic energy due to the turbulence. Conversely, the outflow launched from the resistive accretion disc is mainly accelerated by the magneto-centrifugal force. We also show that when a dense inner stellar wind occurs, the resulting disc-driven jet have a different structure, namely a magnetic structure where poloidal magnetic field lines are more inclined because of the pressure caused by the stellar wind. This modification leads to both an enhanced mass ejection rate in the disc-driven jet and a larger radial extension which is in better agreement with the observations besides being more consistent.Comment: Accepted for publication in Astrophysics & Space Science. Referred proceeding of the fifth Mont Stromlo Symposium Dec. 1-8 2006, Canberra, Australia. 5 pages, 3 figures. For high resolution version of the paper, please click here http://www.apc.univ-paris7.fr/~fcasse/publications.htm

Involvement of a putative intercellular signal-recognizing G protein-coupled receptor in the engulfment of Salmonella by the protozoan Tetrahymena

In an effort to investigate the molecular basis of protozoa engulfment-mediated hypervirulence of Salmonella in cattle, we evaluated protozoan G protein-coupled receptors (GPCRs) as transducers of Salmonella engulfment by the model protozoanTetrahymena. Our laboratory previously demonstrated that non-pathogenic protozoa (including Tetrahymena) engulf Salmonella and then exacerbate its virulence in cattle, but the mechanistic details of the phenomenon are not fully understood. GPCRs were investigated since these receptors facilitate phagocytosis of particulates byTetrahymena, and a GPCR apparently modulates bacterial engulfment for the pathogenic protozoan Entamoeba histolytica. A database search identified three putative Tetrahymena GPCRs, based on sequence homologies and predicted transmembrane domains, that were the focus of this study. Salmonella engulfment by Tetrahymenawas assessed in the presence of suramin, a non-specific GPCR inhibitor. Salmonella engulfment was also assessed in Tetrahymena in which expression of putative GPCRs was knocked-down using RNAi. A candidate GPCR was then expressed in a heterologous yeast expression system for further characterization. Our results revealed that Tetrahymena were less efficient at engulfing Salmonella in the presence of suramin. Engulfment was reduced concordantly with a reduction in the density of protozoa. RNAi-based studies revealed that knock-down of one the Tetrahymena GPCRs caused diminished engulfment of Salmonella. Tetrahymena lysates activated this receptor in the heterologous expression system. These data demonstrate that the Tetrahymena receptor is a putative GPCR that facilitates bacterial engulfment by Tetrahymena. Activation of the putative GPCR seemed to be related to protozoan cell density, suggesting that its cognate ligand is an intercellular signaling molecule

Gravitational vacuum polarization IV: Energy conditions in the Unruh vacuum

Building on a series of earlier papers [gr-qc/9604007, gr-qc/9604008, gr-qc/9604009], I investigate the various point-wise and averaged energy conditions in the Unruh vacuum. I consider the quantum stress-energy tensor corresponding to a conformally coupled massless scalar field, work in the test-field limit, restrict attention to the Schwarzschild geometry, and invoke a mixture of analytical and numerical techniques. I construct a semi-analytic model for the stress-energy tensor that globally reproduces all known numerical results to within 0.8%, and satisfies all known analytic features of the stress-energy tensor. I show that in the Unruh vacuum (1) all standard point-wise energy conditions are violated throughout the exterior region--all the way from spatial infinity down to the event horizon, and (2) the averaged null energy condition is violated on all outgoing radial null geodesics. In a pair of appendices I indicate general strategy for constructing semi-analytic models for the stress-energy tensor in the Hartle-Hawking and Boulware states, and show that the Page approximation is in a certain sense the minimal ansatz compatible with general properties of the stress-energy in the Hartle-Hawking state.Comment: 40 pages; plain LaTeX; uses epsf.sty (ten encapsulated postscript figures); two tables (table and tabular environments). Should successfully compile under both LaTeX 209 and the 209 compatibility mode of LaTeX2

A motif-based approach to network epidemics

Networks have become an indispensable tool in modelling infectious diseases, with the structure of epidemiologically relevant contacts known to affect both the dynamics of the infection process and the efficacy of intervention strategies. One of the key reasons for this is the presence of clustering in contact networks, which is typically analysed in terms of prevalence of triangles in the network. We present a more general approach, based on the prevalence of different four-motifs, in the context of ODE approximations to network dynamics. This is shown to outperform existing models for a range of small world networks

On the Spectral Evolution of Cool, Helium-Atmosphere White Dwarfs: Detailed Spectroscopic and Photometric Analysis of DZ Stars

We present a detailed analysis of a large spectroscopic and photometric sample of DZ white dwarfs based on our latest model atmosphere calculations. We revise the atmospheric parameters of the trigonometric parallax sample of Bergeron, Leggett, & Ruiz (12 stars) and analyze 147 new DZ white dwarfs discovered in the Sloan Digital Sky Survey. The inclusion of metals and hydrogen in our model atmosphere calculations leads to different atmospheric parameters than those derived from pure helium models. Calcium abundances are found in the range from log (Ca/He) = -12 to -8. We also find that fits of the coolest objects show peculiarities, suggesting that our physical models may not correctly describe the conditions of high atmospheric pressure encountered in the coolest DZ stars. We find that the mean mass of the 11 DZ stars with trigonometric parallaxes, = 0.63 Mo, is significantly lower than that obtained from pure helium models, = 0.78 Mo, and in much better agreement with the mean mass of other types of white dwarfs. We determine hydrogen abundances for 27% of the DZ stars in our sample, while only upper limits are obtained for objects with low signal-to-noise ratio spectroscopic data. We confirm with a high level of confidence that the accretion rate of hydrogen is at least two orders of magnitude smaller than that of metals (and up to five in some cases) to be compatible with the observations. We find a correlation between the hydrogen abundance and the effective temperature, suggesting for the first time empirical evidence of a lower temperature boundary for the hydrogen screening mechanism. Finally, we speculate on the possibility that the DZA white dwarfs could be the result of the convective mixing of thin hydrogen-rich atmospheres with the underlying helium convection zone.Comment: 67 pages, 32 figures, accepted for publication in Ap

The monoenergetic approximation in stellarator neoclassical calculations

In the standard "monoenergetic" approach to numerical calculation of stellarator neoclassical transport, to expedite computation, ad-hoc changes are made to the kinetic equation so speed enters only as a parameter. Here we examine the validity of this approach by considering the effective particle trajectories in a model magnetic field. We find monoenergetic codes systematically under-predict the true trapped particle fraction, with the error in the trapped ion fraction being of order unity when the electric field is large, suggesting some results of these codes may be unreliable in this regime. This inaccuracy is independent of any errors introduced by approximation of the collision operator.Comment: 8 pages, 2 figures, submitted to Plasma Phys. Controlled Fusio