The Nutritional Dynamic is Key for Use Optimal Forage and Increase of Meat Production

The nutritional content grass could be considered a key tool to determine the optimal forage use, based on the requirements of the cattle to maximize production and achieve a highly productive and profitable livestock. The degradation protein complex associated with autophagy plant determines to a great extent the protein content of the grass over time, being priority found the value nutritional required for the livestock for intensification the animal production. The crude protein requirements (CP, 13.5%) to cover nutritional needs in cattle, was established between 28±1 y 30±1 for the dry period and wet period respectively in Camello® hybrid grass. The weight gains to level protein above mentioned were substantially high in both periods. In dry period was 0.9 kg d-1 animal-1 and wet period 1.1 kg d-1 animal-1. The little difference in weight gain between periods clarifies our hypothesis

Nutritional Sensitivity Per Morphological Component in \u3ci\u3eUrochloa\u3c/i\u3e Hybrid under Tropical Environments

Camello® (GP 3025) is a tolerant to drought Urochloa hybrid grass showing good forage production, early flowering with high regrowth rates. The objective was to define nutrient concentration changes by morphological component and, their differences for two tropical contrasting environments. Nutrient concentrations differences (P≤0.05) were observed among morphological components over time. In environments (Aw1) the morphological components showed higher average protein content in comparison to that from hostile conditions (Aw0); lamina (12.2% vs 10.4%), pseudostem (9.5% vs 6.6%) and sheath (9.8% vs 6.8%). Hostile environments (Aw0) promoted increases for FAD (47.9%, 46.9%, pseudostem and sheath, respectively) and lignin (6.6%, 9.1% pseudostem and sheath, respectively). The nutritional changes per morphological components is the best tool to define optimal moment for harvesting on nutritional bioavailability for livestock production intensification

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

The Helicobacter pylori Genome Project : insights into H. pylori population structure from analysis of a worldwide collection of complete genomes

Helicobacter pylori, a dominant member of the gastric microbiota, shares co-evolutionary history with humans. This has led to the development of genetically distinct H. pylori subpopulations associated with the geographic origin of the host and with differential gastric disease risk. Here, we provide insights into H. pylori population structure as a part of the Helicobacter pylori Genome Project (HpGP), a multi-disciplinary initiative aimed at elucidating H. pylori pathogenesis and identifying new therapeutic targets. We collected 1011 well-characterized clinical strains from 50 countries and generated high-quality genome sequences. We analysed core genome diversity and population structure of the HpGP dataset and 255 worldwide reference genomes to outline the ancestral contribution to Eurasian, African, and American populations. We found evidence of substantial contribution of population hpNorthAsia and subpopulation hspUral in Northern European H. pylori. The genomes of H. pylori isolated from northern and southern Indigenous Americans differed in that bacteria isolated in northern Indigenous communities were more similar to North Asian H. pylori while the southern had higher relatedness to hpEastAsia. Notably, we also found a highly clonal yet geographically dispersed North American subpopulation, which is negative for the cag pathogenicity island, and present in 7% of sequenced US genomes. We expect the HpGP dataset and the corresponding strains to become a major asset for H. pylori genomics

Spatiotemporal Characteristics of the Largest HIV-1 CRF02_AG Outbreak in Spain: Evidence for Onward Transmissions

Background and Aim: The circulating recombinant form 02_AG (CRF02_AG) is the predominant clade among the human immunodeficiency virus type-1 (HIV-1) non-Bs with a prevalence of 5.97% (95% Confidence Interval-CI: 5.41–6.57%) across Spain. Our aim was to estimate the levels of regional clustering for CRF02_AG and the spatiotemporal characteristics of the largest CRF02_AG subepidemic in Spain.Methods: We studied 396 CRF02_AG sequences obtained from HIV-1 diagnosed patients during 2000–2014 from 10 autonomous communities of Spain. Phylogenetic analysis was performed on the 391 CRF02_AG sequences along with all globally sampled CRF02_AG sequences (N = 3,302) as references. Phylodynamic and phylogeographic analysis was performed to the largest CRF02_AG monophyletic cluster by a Bayesian method in BEAST v1.8.0 and by reconstructing ancestral states using the criterion of parsimony in Mesquite v3.4, respectively.Results: The HIV-1 CRF02_AG prevalence differed across Spanish autonomous communities we sampled from (p < 0.001). Phylogenetic analysis revealed that 52.7% of the CRF02_AG sequences formed 56 monophyletic clusters, with a range of 2–79 sequences. The CRF02_AG regional dispersal differed across Spain (p = 0.003), as suggested by monophyletic clustering. For the largest monophyletic cluster (subepidemic) (N = 79), 49.4% of the clustered sequences originated from Madrid, while most sequences (51.9%) had been obtained from men having sex with men (MSM). Molecular clock analysis suggested that the origin (tMRCA) of the CRF02_AG subepidemic was in 2002 (median estimate; 95% Highest Posterior Density-HPD interval: 1999–2004). Additionally, we found significant clustering within the CRF02_AG subepidemic according to the ethnic origin.Conclusion: CRF02_AG has been introduced as a result of multiple introductions in Spain, following regional dispersal in several cases. We showed that CRF02_AG transmissions were mostly due to regional dispersal in Spain. The hot-spot for the largest CRF02_AG regional subepidemic in Spain was in Madrid associated with MSM transmission risk group. The existence of subepidemics suggest that several spillovers occurred from Madrid to other areas. CRF02_AG sequences from Hispanics were clustered in a separate subclade suggesting no linkage between the local and Hispanic subepidemics

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Patrón de crecimiento estacional de pastos nativos, en un bosque de encino, en el Estado de México

El conocimiento de los cambios en la producción de forraje de pastos nativos en áreas forestales durante la estación de crecimiento, es una herramienta útil para un mejor aprovechamiento de los pastizales. El objetivo de este estudio fue determinar la curva de acumulación de forraje, tasa de crecimiento promedio y proteína total a diferentes edades de rebrote en pastos nativos, en tres áreas con diferentes densidades de árboles y arbustos: alta en árboles y alta en arbustos (AA+ Aa), intermedia en árboles y baja en arbustos (IA+Ba), y baja en árboles e intermedia en arbustos (BA+Ia), en el Estado de México. Se utilizó un diseño de bloques completos al azar con cuatro repeticiones. En 2001 y 2002 Brachypodium mexicanum presentó la mayor masa de forraje y tasa de crecimiento promedio en el área AA+Aa, mientras que para el área BA+Ia las registraron Muhlenbergia rigida y Piptochaetium fimbriatum. El mayor contenido de proteína total se encontró en P. fimbriatum a los 20 d de rebrote

Rendimiento y contenido de proteína en forraje y ensilado de pasto Insurgente e híbridos de Urochloa

Livestock in the state of Tamaulipas is affected due to a marked seasonality in forage production, which causes shortages during the dry season, therefore, forage conservation is necessary to face this problem. Therefore, the objective of this study was to evaluate, in stormy conditions and during the season of greatest rainfall, the yield (t ha-1) of total dry matter (TDM) and leaf (DML); as well as, the crude protein content (CP,%) in forage and silage of Insurgente, MulatoII, Cobra and Cayman grass. The data were analyzed in a completely randomized design, with four repetitions and comparison of means using Tukey (p= 0.05). The Cayman hybrid presented higher accumulation (p≤0.05) of TDMand DML(8.34 and 8.27 t ha-1, respectively) compared to Insurgente(6.38 and 6.25 t ha-1, respectively). The highest (p≤ 0.05) content of CP in forage was presented by Cayman and Cobra, with 16.1% on average, while Mulato II and Insurgentewere those with the lowest value for this variable (14.6%, on average). The CP in the silage was similar (p˃0.05) for the cultivars evaluated, with an average of 14.4%. The Cayman and Cobra hybrids showed higher forage yield and crude protein content, compared to Insurgente. The crude protein content present in the silage was greater than 14%, which indicates that these cultivars can be preserved using this technique.La ganadería en el estado de Tamaulipas se ve afectada debido a una marcada estacionalidad en la producción de forraje, que ocasiona escasez durante la época seca, por tanto, es necesario la conservación de forraje para hacer frente a este problema. Por lo anterior, el objetivo de este estudio fue evaluar en condiciones de temporal y durante la época de mayor precipitación el rendimiento (t ha-1) de materia seca total (MST) y hoja (MSH); así como, el contenido de proteína cruda (PC, %) en forraje y ensilado de pasto Insurgente, Mulato II, Cobra y Cayman. Los datos se analizaron en un diseño completamente al azar, con cuatro repeticiones y comparación de medias mediante Tukey (p= 0.05). El híbrido Cayman presentó mayor acumulación (p≤ 0.05) de MST y MSH (8.34 y 8.27 t ha-1, respectivamente) en comparación con Insurgente (6.38 y 6.25 t ha-1, respectivamente). El mayor (p≤ 0.05) contenido de PC en forraje lo presentaron Cayman y Cobra, con 16.1% en promedio, mientras que Mulato II e Insurgente fueron los de menor valor para esta variable (14.6%, en promedio). La PC en el ensilado fue similar (p˃ 0.05) para los cultivares evaluados, con promedio de 14.4%. Los híbridos Cayman y Cobra mostraron mayor rendimiento de forraje y contenido de proteína cruda, en comparación con Insurgente. El contenido de proteína cruda presente en el ensilado fue superior al 14%, lo que indica que estos cultivares se pueden conservar mediante esta técnica

erminación y crecimiento de plántulas de Phaseolus vulgaris L. en condiciones de salinidad*

Beans are sensitive to salinity. In Mexico in the autumn-winter cycle, bean production is performed under conditions of residual moisture. Under these conditions, salinity problems are increased and are reflected in the reduction in the percentage of germination, growth and underground and aerial performance. The levels of damage are different between varieties. In this research, conducted in Nayarit in 2012 in a greenhouse at a temperature of 28.1 °C, the physiological response to salinity induced with sodium chloride, three bean varieties of higher production in Nayarit in the germination stage and seedling was evaluated. The experimental design was completely randomized using three replicates per treatment. Pinto bean germination decreased 54.7% to 9 dS m-1 and beans Azufrado 30.3%. In seedlings, the stem length difference between the control (T0) and the highest treatment (T6) was 2.4, 4 and 3.2 cm for the Negro, Pinto and Azufrado respectively beans. The difference in root length between T0 and T6, was 13.1, 13.4 and 12.1 cm for the Negro, Pinto and Azufrado respectively beans. It as the electrical conductivity increased germination rate decreased. The Azufrado beans were the toughest and the most affected Pinto. The stem length, fresh weight and dry weight of seedling decreased and increased stem diameter. The length, fresh weight and dry root weight decreased gradually.El frijol es sensible a la salinidad. En México en el ciclo otoño-invierno, la producción del frijol se realiza en condiciones de humedad residual. En estas condiciones los problemas de salinidad se incrementan y se manifiesta en la reducción del porcentaje de germinación, el crecimiento aéreo y subterráneo y el rendimiento. Los grados de afectación son diferentes entre variedades. En ésta investigación, realizada en Nayarit en 2012 en invernadero a una temperatura de 28.1 °C, se evaluó la respuesta fisiológica a la salinidad, inducida con cloruro de sodio, de tres variedades de frijol de mayor producción en Nayarit en la etapa de germinación y plántula. El diseño experimental fue completamente al azar empleando tres repeticiones por tratamiento. La germinación del frijol Pinto se redujo 54.7% a 9 dS m-1 y el frijol Azufrado 30.3%. En plántula, la diferencia de longitud de tallo entre el testigo (T0) y el tratamiento más alto (T6) fue de 2.4, 4 y 3.2 cm para el frijol Negro, Pinto y Azufrado, respectivamente. La diferencia en longitud de raíz entre T0 y T6, fue de 13.1, 13.4 y 12.1 cm para el frijol Negro, Pinto y Azufrado, respectivamente. Conforme la conductividad eléctrica se incrementó la tasa de germinación disminuyó. El frijol Azufrado fue el más resistente y el Pinto el más afectado. La longitud de tallo, el peso fresco y peso seco de la plántula disminuyó y el diámetro de tallo incrementó. La longitud, peso fresco y peso seco de la raíz disminuyó gradualmente

GWAS and meta-analysis identifies 49 genetic variants underlying critical COVID-19

Data availability: Downloadable summary data are available through the GenOMICC data site (https://genomicc.org/data). Summary statistics are available, but without the 23andMe summary statistics, except for the 10,000 most significant hits, for which full summary statistics are available. The full GWAS summary statistics for the 23andMe discovery dataset will be made available through 23andMe to qualified researchers under an agreement with 23andMe that protects the privacy of the 23andMe participants. For further information and to apply for access to the data, see the 23andMe website (https://research.23andMe.com/dataset-access/). All individual-level genotype and whole-genome sequencing data (for both academic and commercial uses) can be accessed through the UKRI/HDR UK Outbreak Data Analysis Platform (https://odap.ac.uk). A restricted dataset for a subset of GenOMICC participants is also available through the Genomics England data service. Monocyte RNA-seq data are available under the title ‘Monocyte gene expression data’ within the Oxford University Research Archives (https://doi.org/10.5287/ora-ko7q2nq66). Sequencing data will be made freely available to organizations and researchers to conduct research in accordance with the UK Policy Framework for Health and Social Care Research through a data access agreement. Sequencing data have been deposited at the European Genome–Phenome Archive (EGA), which is hosted by the EBI and the CRG, under accession number EGAS00001007111.Extended data figures and tables are available online at https://www.nature.com/articles/s41586-023-06034-3#Sec21 .Supplementary information is available online at https://www.nature.com/articles/s41586-023-06034-3#Sec22 .Code availability: Code to calculate the imputation of P values on the basis of SNPs in linkage disequilibrium is available at GitHub (https://github.com/baillielab/GenOMICC_GWAS).Acknowledgements: We thank the members of the Banco Nacional de ADN and the GRA@CE cohort group; and the research participants and employees of 23andMe for making this work possible. A full list of contributors who have provided data that were collated in the HGI project, including previous iterations, is available online (https://www.covid19hg.org/acknowledgements).Change history: 11 July 2023: A Correction to this paper has been published at: https://doi.org/10.1038/s41586-023-06383-z. -- In the version of this article initially published, the name of Ana Margarita Baldión-Elorza, of the SCOURGE Consortium, appeared incorrectly (as Ana María Baldion) and has now been amended in the HTML and PDF versions of the article.Copyright © The Author(s) 2023, Critical illness in COVID-19 is an extreme and clinically homogeneous disease phenotype that we have previously shown1 to be highly efficient for discovery of genetic associations2. Despite the advanced stage of illness at presentation, we have shown that host genetics in patients who are critically ill with COVID-19 can identify immunomodulatory therapies with strong beneficial effects in this group3. Here we analyse 24,202 cases of COVID-19 with critical illness comprising a combination of microarray genotype and whole-genome sequencing data from cases of critical illness in the international GenOMICC (11,440 cases) study, combined with other studies recruiting hospitalized patients with a strong focus on severe and critical disease: ISARIC4C (676 cases) and the SCOURGE consortium (5,934 cases). To put these results in the context of existing work, we conduct a meta-analysis of the new GenOMICC genome-wide association study (GWAS) results with previously published data. We find 49 genome-wide significant associations, of which 16 have not been reported previously. To investigate the therapeutic implications of these findings, we infer the structural consequences of protein-coding variants, and combine our GWAS results with gene expression data using a monocyte transcriptome-wide association study (TWAS) model, as well as gene and protein expression using Mendelian randomization. We identify potentially druggable targets in multiple systems, including inflammatory signalling (JAK1), monocyte–macrophage activation and endothelial permeability (PDE4A), immunometabolism (SLC2A5 and AK5), and host factors required for viral entry and replication (TMPRSS2 and RAB2A).GenOMICC was funded by Sepsis Research (the Fiona Elizabeth Agnew Trust), the Intensive Care Society, a Wellcome Trust Senior Research Fellowship (to J.K.B., 223164/Z/21/Z), the Department of Health and Social Care (DHSC), Illumina, LifeArc, the Medical Research Council, UKRI, a BBSRC Institute Program Support Grant to the Roslin Institute (BBS/E/D/20002172, BBS/E/D/10002070 and BBS/E/D/30002275) and UKRI grants MC_PC_20004, MC_PC_19025, MC_PC_1905 and MRNO2995X/1. A.D.B. acknowledges funding from the Wellcome PhD training fellowship for clinicians (204979/Z/16/Z), the Edinburgh Clinical Academic Track (ECAT) programme. This research is supported in part by the Data and Connectivity National Core Study, led by Health Data Research UK in partnership with the Office for National Statistics and funded by UK Research and Innovation (grant MC_PC_20029). Laboratory work was funded by a Wellcome Intermediate Clinical Fellowship to B.F. (201488/Z/16/Z). We acknowledge the staff at NHS Digital, Public Health England and the Intensive Care National Audit and Research Centre who provided clinical data on the participants; and the National Institute for Healthcare Research Clinical Research Network (NIHR CRN) and the Chief Scientist’s Office (Scotland), who facilitate recruitment into research studies in NHS hospitals, and to the global ISARIC and InFACT consortia. GenOMICC genotype controls were obtained using UK Biobank Resource under project 788 funded by Roslin Institute Strategic Programme Grants from the BBSRC (BBS/E/D/10002070 and BBS/E/D/30002275) and Health Data Research UK (HDR-9004 and HDR-9003). UK Biobank data were used in the GSMR analyses presented here under project 66982. The UK Biobank was established by the Wellcome Trust medical charity, Medical Research Council, Department of Health, Scottish Government and the Northwest Regional Development Agency. It has also had funding from the Welsh Assembly Government, British Heart Foundation and Diabetes UK. The work of L.K. was supported by an RCUK Innovation Fellowship from the National Productivity Investment Fund (MR/R026408/1). J.Y. is supported by the Westlake Education Foundation. SCOURGE is funded by the Instituto de Salud Carlos III (COV20_00622 to A.C., PI20/00876 to C.F.), European Union (ERDF) ‘A way of making Europe’, Fundación Amancio Ortega, Banco de Santander (to A.C.), Cabildo Insular de Tenerife (CGIEU0000219140 ‘Apuestas científicas del ITER para colaborar en la lucha contra la COVID-19’ to C.F.) and Fundación Canaria Instituto de Investigación Sanitaria de Canarias (PIFIISC20/57 to C.F.). We also acknowledge the contribution of the Centro National de Genotipado (CEGEN) and Centro de Supercomputación de Galicia (CESGA) for funding this project by providing supercomputing infrastructures. A.D.L. is a recipient of fellowships from the National Council for Scientific and Technological Development (CNPq)-Brazil (309173/2019-1 and 201527/2020-0)