Transient UV-vis absorption spectroscopic characterisation of 2 '-methoxyacetophenone as a DNA photosensitiser

[EN] 2'-Methoxyacetophenone (2M) presents improved UVA absorption as compared with other acetophenone derivatives. On the basis of transient infrared spectroscopy it has been previously claimed that 2M is an interesting photosensitiser for cyclobutane pyrimidine dimers (CPDs) formation. In the present paper, a complete UV-Vis transient absorption spectroscopic characterisation of this compound is provided, including triplet-triplet spectra, triplet lifetimes and rate constants for quenching of 2M by a dimeric thymine derivative. Furthermore, generation of singlet oxygen has been proven by time-resolved near IR phosphorescence measurements. Overall, the obtained results confirm the potential of 2M as a DNA photosensitiser, not only for CPDs formation, but also for oxidative damage.Financial support by the Spanish Government (O.R-A., FPU14/05294 and CTQ2015-70164-P) and Generalitat Valenciana (PROMETEO/2017/075) is gratefully acknowledged.Rodriguez-Alzueta, O.; Cuquerella Alabort, MC.; Miranda Alonso, MÁ. (2019). Transient UV-vis absorption spectroscopic characterisation of 2 '-methoxyacetophenone as a DNA photosensitiser. Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy. 218:191-195. https://doi.org/10.1016/j.saa.2019.04.007S19119521

Triplet Energy Transfer versus Excited State Cyclization as the Controlling Step in Photosensitized Bipyrimidine Dimerization

[EN] Polymethylene-linked bipyrimidine models have been designed with different C5 substitutions and bridge lengths. Selective irradiation of 2'-methoxyacetophenone (2M) with the bipyrimidine models affords cyclobutane pyrimidine dimers, even in the presence of bulky substituents. Substitution at CS affects both the relative triplet energies (E-T(rel)) of the pyrimidines (Pyr) and the steric hindrance toward intermolecular energy transfer and intramolecular triplet Pyr* quenching. Photophysical studies showed that alkyl substitution resulted in a significant decrease in the E-T(rel) value. Quenching of the triplet excited state of 2M by the Pyr derivatives was proven and established their quenching rate constants (k(q)). As a general trend, the thymine-containing compounds showed k(q) values higher than 10(9) M(-1)s(-1), while in the uracil and tert-butyluracil analogues, k(q) was markedly lower. These data are explained considering three different scenarios: (a) triplet energy transfer is the rate controlling step, (b) excited state cyclization is the rate controlling step, and (c) the rate controlling step switches along the reaction. Thus, by introducing variations in the substitution at C5, the length of the linking bridge, or the substrate concentration, it is possible to switch from a process governed by the intrinsic dimerization step to an energy transfer-controlled process.Financial support by the Spanish Government (PGC2018-096684 and O.R.A. for FPU14/05294) and Generalitat Valenciana (PROMETEO/2017/075) is gratefully acknowledged.Rodriguez-Alzueta, O.; Cuquerella Alabort, MC.; Miranda Alonso, MÁ. (2019). Triplet Energy Transfer versus Excited State Cyclization as the Controlling Step in Photosensitized Bipyrimidine Dimerization. The Journal of Organic Chemistry. 84(21):13329-13335. https://doi.org/10.1021/acs.joc.9b01423S1332913335842

Survivability of entrapped Lactobacillus rhamnosus in liquid- and gel-core alginate beads during storge and simulated gastrointestinal conditions

L. rhamnosus cells were encapsulated in liquid-core (LCBR) and gel-core (GCBR) calcium alginate beads, and cell survivability under storage conditions and simulated gastrointestinal conditions were evaluated, and compared with that of non-encapsulated cells. The average external diameters of both beads (1.37 - 0.25 mm) were non-significantly dierent, and the average thickness of alginate gelled layer in LCBR was of 0.27 - 0.01 mm. The bacteria entrapped into LCBR tended to gather together forming clusters in the bulk of the liquid phase of the bead, whereas the bacteria entrapped into GCBR were compartmentalized in the gelled bead biopolymer matrix. LCBR showed significant lower hardness and chewiness, higher cohesiveness, and comparable springiness values than GCBR. Cells survivability under storage and simulated gastrointestinal conditions was significantly higher in LCBR than in GCBR and for the non-encapsulated free cells

Genotipos de virus papiloma humano en mujeres con alteraciones citológicas cervicales de un área con alta incidencia de cáncer cervical

It has been well demonstrated the relationship between the infection with high-risk human papillomavirus (HPVs) genotypes and cervical cancer. In Northeastern Argentina a high incidence of this pathology has been described and therefore a high prevalence of HPV infection is expected. In order to identify HPV genotypes associated with malignant and pre-malignant cervical lesions present in the area, 53 ecto-endo cervical cell specimens obtained from women with cytohistological alterations were studied by a PCR-RFLP technique. Out of 53 patients, 34 (64.2%) were positive for HPV infection, being HPV-16 (32.3%) the most frequently found genotype, followed by HPV-58 (14.7%), -6, -18 and -45 (5.9%), -33, -52, -53, -54, -56, -66, -MM4 and -LVX100 (2.9%). Also 5 cases of infection caused by multiple genotypes were found, which corresponded to 14.7% of the positive cases. Results indicate that besides HPV-16 and -18, the most prevalent high-risk HPV genotypes worldwide, others like -45 and -58 as well as co-infection cases are frequent between women of Northeastern Argentina, and a particular attention should be paid to this circumstance because it could be an epidemiological feature of regional importance and a useful information for a future vaccination program.La relación entre la infección por los virus papiloma humanos (HPVs) de alto riesgo y el cáncer de cuello de útero ha sido bien demostrada. En el Nordeste de Argentina se observa una alta incidencia de esta patología y en consecuencia se estima una alta prevalencia de infección por HPV. A fin de identificar los genotipos de HPV presentes en el área, asociados a casos de lesiones malignas y premalignas de cuello de útero, se estudiaron 53 muestras ecto-endo cervicales de mujeres con alteraciones citohistológicas residentes permanentes de las ciudades de Resistencia y Corrientes. De las 53 pacientes estudiadas, 34 resultaron positivas para HPV (64.2%), correspondiendo la mayor frecuencia a HPV-16 (32.3%), seguido por HPV-58 (14.7%), HPV-6, -18 y -45 (5.9%), -33, -52, -53, -54, -56, -66, -MM4 y -LVX100 (2.9%). Además, se encontraron 5 casos de infecciones mixtas causadas por mas de un genotipo, lo que resulta de importancia ya que representan el 14.7% del total de los casos positivos. Los resultados demuestran que, además de HPV-16 y -18 que son los genotipos de alto riesgo de mayor prevalencia a nivel mundial, otros como el HPV-45 y -58 y los casos de infecciones múltiples son frecuentes en mujeres del nordeste argentino, lo que podría constituir un rasgo epidemiológico de importancia regional y ser de utilidad en el futuro en los programas de vacunación

Implementación de un modelo de cultura organizacional en la empresa Para Todos, ubicada en le ciudad de Mocoa, que promueva su desarrollo integral para el segundo semestre del año 2022

El Presente trabajo de investigación hace énfasis en definir un modelo de cultura organizacional para la empresa “Autoservicio para todos”, la empresa se encuentra ubicada en la ciudad de Mocoa Putumayo. Es una actividad que se desarrolla durante el segundo periodo del 2022, con lo cual, se busca que la empresa tenga una identidad organizacional que le permita actuar de forma estratégica y eficiente a través de los valores empresariales, que promuevan la competitividad, el logro de los objetivos y reconocimiento en el mercado; factores que constituyen la mejora continua que busca la organización para el crecimiento progresivo de la empresa y su permanencia en el tiempo. La empresa “Autoservicio para todos” para definir la cultura organizacional, estudia y se apoya en las diferentes teorías de la evolución y su impacto en la evolución del siglo XXI, Teorías de enfoque hacia una organización responsable, incluyente con cada uno de los trabajadores para un trabajo en equipo y el logro de objetivos empresariales. El método usado para el estudio de la propuesta de investigación, que concierne a la implementación de cultura organizacional en la empresa “autoservicio para todos” se trata de una recolección de datos que se realiza usando un instrumento eficiente como lo es una encuesta en línea, con preguntas cerradas, lo cual fue aplicada a todos los empleados de la empresa objeto de estudio, para detectar la información más relevante de cómo se está ejecutando la cultura organizacional actualmente, para luego proponer soluciones y recomendaciones sobre como la empresa debe actuar para mejorar los problemas que se presenten en la cultura organizacional. Según el estudio realizado, los resultados obtenidos son muy importantes y nos demuestran que la empresa tiene algunas falencias que implican a la cultura organización, falencias en normas de conducta, trabajo en equipo, los trabajadores no conocen la misión, visión y valores de la empresa y falta capacitaciones son algunos aspectos, sin embrago existen otros resultados que benefician a la empresa, concluimos que la empresa le falta mejorar algunas cosas de la cultura organizacional pero que no son tan graves y se pueden mejorar perfectamente.This research work emphasizes defining a model of organizational culture for the company "Self-service for all", the company is located in the city of Mocoa Putumayo. It is an activity that takes place during the second period of 2022, with which it is sought that the company has an organizational identity that allows it to act strategically and efficiently through business values, which promote competitiveness, the achievement of the objectives and recognition in the market; factors that constitute the continuous improvement that the organization seeks for the progressive growth of the company and its permanence over time. The company "Self-service for all" to define the organizational culture, studies and relies on the different theories of evolution and its impact on the evolution of the 21st century, Theories of focus towards a responsible organization, inclusive of each one of the workers to teamwork and achievement of business goals. The method used for the study of the research proposal, which concerns the implementation of organizational culture in the company "self-service for all" is a data collection that is carried out using an efficient instrument such as an online survey, with closed questions, which was applied to all the employees of the company under study, to detect the most relevant information on how the organizational culture is currently running, to then propose solutions and recommendations on how the company should act to improve the results. problems that arise in the organizational culture. According to the study carried out, the results obtained are very important and show us that the company has some shortcomings that involve the organizational culture, shortcomings in standards of conduct, teamwork, workers do not know the mission, vision and values of the company. and training is lacking in some aspects, however there are other results that benefit the company, we conclude that the company needs to improve some things in the organizational culture but that they are not so serious and can be perfectly improved

RIG-I contributes to the innate immune response after cerebral ischemia

BACKGROUND: Focal cerebral ischemia induces an inflammatory response that when exacerbated contributes to deleterious outcomes. The molecular basis regarding the regulation of the innate immune response after focal cerebral ischemia remains poorly understood. METHODS: In this study we examined the expression of retinoic acid-inducible gene (RIG)-like receptor-I (RIG-I) and its involvement in regulating inflammation after ischemia in the brain of rats subjected to middle cerebral artery occlusion (MCAO). In addition, we studied the regulation of RIG-I after oxygen glucose deprivation (OGD) in astrocytes in culture. RESULTS: In this study we show that in the hippocampus of rats, RIG-I and IFN-α are elevated after MCAO. Consistent with these results was an increased in RIG-I and IFN-α after OGD in astrocytes in culture. These data are consistent with immunohistochemical analysis of hippocampal sections, indicating that in GFAP-positive cells there was an increase in RIG-I after MCAO. In addition, in this study we have identified n-propyl gallate as an inhibitor of IFN-α signaling in astrocytes. CONCLUSION: Our findings suggest a role for RIG-I in contributing to the innate immune response after focal cerebral ischemia

Conexión inteligente para la gestión turística desde el aula al destino (CIGTAD – II)

Para reducir la brecha digital existente en la sociedad actual es necesario situar la innovación en el núcleo de la toma de decisiones. Este proyecto camina hacia la plena inclusión de todos los colectivos de la comunidad universitaria en el uso de las nuevas tecnologías, y gracias a ellas, poder generar una mayor conciencia social y medioambiental. Desarrollaremos soluciones para la mejora de la accesibilidad a la información que se genera, tanto formal como informal, para los grupos implicados y en cuyos contenidos se introducirá la conceptualización de los ODS y su implicación en sectores tan importantes como el Comercio y el Turismo. Se trabajará con tecnología actual y futura de los destinos turísticos, lo que mejorará, a su vez, la empleabilidad de los egresados en la facultad. El proyecto impulsa la implementación de las tecnologías que se están utilizando en los planes estratégicos de Destinos Turísticos Inteligentes. De este modo, los estudiantes del Grado en Turismo y el Doble Grado en Turismo y Comercio, así como del Máster en Planificación y Gestión de Destinos Turísticos podrán experimentar la inmersión en el uso de este tipo de herramientas. El proyecto estará dividido en 3 fases como son: el análisis, el desarrollo de contenidos y la implementación de las herramientas en el que participarán diferentes colectivos tales como PDI, PAS y Estudiantes de diferentes niveles y Alumni de la Facultad de Comercio y Turismo, así como la participación de una trabajadora social experta en trabajar con colectivos juveniles. El proyecto está alineado para trabajar por mejorar la sostenibilidad de la facultad e incrementar el nivel de compromiso de los colectivos con los objetivos de la Agenda 2030

Conexión inteligente para la gestión turística desde el aula al destino (CIGTAD)

Fac. de Comercio y TurismoFac. de Trabajo SocialFALSEsubmitte

New World Civitas, Contested Jurisdictions and Intercultural Conversation in the Construction of the Spanish Monarchy

Jurisdictional frontiers were created, contested, and negotiated among a wide range of actors, including native Americans and Europeans, with reference to the cities founded in Castilla del Oro (roughly present-day Panama). This research deals, first, with the reshaping of the concept of a city in the New World, based on its inhabitants' sense of civitas. It analyses, secondly, the creation and redefinition of jurisdiction during political conflicts and, third, the construction and maintenance of jurisdiction through local relations with indigenous populations described as "conversation". The analysis of the creation and preservation of local jurisdictions allows for an interpretation of the complexities involved in the configuration of political power and political space from below in the territories claimed by the Spanish Monarchy.Art Empir