Biomechanical demands of the 2-step transitional gait cycles linking level gait and stair descent gait in older women

Stair descent is an inherently complex form of locomotion posing a high falls risk for older adults, specifically when negotiating the transitional gait cycles linking level gait and descent. The aim of this study was to enhance our understanding of the biomechanical demands by comparing the demands of these transitions. Lower limb kinematics and kinetics of the 2-step transitions linking level and descent gait at the top (level-to-descent) and the bottom (descent-to-level) of the staircase were quantified in 36 older women with no falls history. Despite undergoing the same vertical displacement (2-steps), the following significant (p<.05) differences were observed during the top transition compared to the bottom transition: reduced step velocity; reduced hip extension and increased ankle dorsiflexion (late stance/pre-swing); reduced ground reaction forces, larger knee extensor moments and powers (absorption; late stance); reduced ankle plantarflexor moments (early and late stance) and increased ankle powers (mid-stance). Top transition biomechanics were similar to those reported previously for continuous descent. Kinetic differences at the knee and ankle signify the contrasting and prominent functions of controlled lowering during the top transition and forward continuance during the bottom transition. The varying musculoskeletal demands encountered during each functional sub-task should be addressed in falls prevention programmes with elderly populations where the greatest clinical impact may be achieved. Knee extensor eccentric power through flexion exercises would facilitate a smooth transition at the top and improving ankle plantarflexion strength during single and double limb stance activities would ease the transition into level gait following continuous descent

Efficient depletion of host DNA contamination in malaria clinical sequencing.

The cost of whole-genome sequencing (WGS) is decreasing rapidly as next-generation sequencing technology continues to advance, and the prospect of making WGS available for public health applications is becoming a reality. So far, a number of studies have demonstrated the use of WGS as an epidemiological tool for typing and controlling outbreaks of microbial pathogens. Success of these applications is hugely dependent on efficient generation of clean genetic material that is free from host DNA contamination for rapid preparation of sequencing libraries. The presence of large amounts of host DNA severely affects the efficiency of characterizing pathogens using WGS and is therefore a serious impediment to clinical and epidemiological sequencing for health care and public health applications. We have developed a simple enzymatic treatment method that takes advantage of the methylation of human DNA to selectively deplete host contamination from clinical samples prior to sequencing. Using malaria clinical samples with over 80% human host DNA contamination, we show that the enzymatic treatment enriches Plasmodium falciparum DNA up to ∼9-fold and generates high-quality, nonbiased sequence reads covering >98% of 86,158 catalogued typeable single-nucleotide polymorphism loci

More sustainable vegetable oil: Balancing productivity with carbon storage opportunities

Intensive cultivation and post-harvest vegetable oil production stages are major sources of greenhouse gas (GHG) emissions. Variation between production systems and reporting disparity have resulted in discordance in previous emissions estimates. The aim of this study was to assess global systems-wide variation in GHG emissions resulting from palm, soybean, rapeseed and sunflower oil production. Such an analysis is critical to understand the implications of meeting increasing edible oil demand. To achieve this, we performed a unified re-analysis of life cycle input data from diverse palm, soybean, rapeseed, and sunflower oil production systems, from a saturating search of published literature. The resulting dataset reflects almost 6000 producers in 38 countries, and is representative of over 71% of global vegetable oil production. Across all oil crop systems, median GHG emissions were 3.81 kg CO2e per kg refined oil. Crop specific median emissions ranged from 2.49 kg CO2e for rapeseed oil to 4.25 kg CO2e for soybean oil per kg refined oil. Determination of the carbon cost of agricultural land occupation revealed that carbon storage potential in native compared to agricultural land cover drives variation in production GHG emissions, and indicates that expansion of production in low carbon storage potential land, whilst reforesting areas of high carbon storage potential, could reduce net GHG emissions whilst boosting productivity. Nevertheless, there remains considerable scope to improve sustainability within current production systems, including through increasing yields whilst limiting application of inputs with high carbon footprints, and in the case of palm oil through more widespread adoption of methane capture technologies in processing stages

Agronomic biofortification of cowpea with selenium: effects of selenate and selenite applications on selenium and phytate concentrations in seeds

BACKGROUNDSelenium (Se) is a nutrient for animals and humans, and is considered beneficial to higher plants. Selenium concentrations are low in most soils, which can result in a lack of Se in plants, and consequently in human diets. Phytic acid (PA) is the main storage form of phosphorus in seeds, and it is able to form insoluble complexes with essential minerals in the monogastric gut. This study aimed to establish optimal levels of Se application to cowpea, with the aim of increasing Se concentrations. The efficiency of agronomic biofortification was evaluated by the application of seven levels of Se (0, 2.5, 5, 10, 20, 40, and 60 g ha−1) from two sources (selenate and selenite) to the soil under field conditions in 2016 and 2017.RESULTSApplication of Se as selenate led to greater plant Se concentrations than application as selenite in both leaves and grains. Assuming human cowpea consumption of 54.2 g day−1, Se application of 20 g ha−1 in 2016 or 10 g ha−1 in 2017 as selenate would have provided a suitable daily intake of Se (between 20 and 55 μg day−1) for humans. Phytic acid showed no direct response to Se application.CONCLUSIONSelenate provides greater phytoavailability than selenite. The application of 10 g Se ha−1 of selenate to cowpea plants could provide sufficient seed Se to increase daily human intake by 13–14 μg d−1. © 2019 Society of Chemical Industr

Age-related changes in physical functioning: Correlates between objective and self-reported outcomes

Objectives: To quantify the variance attributable to age and estimate annual decline in physical function and self-reported health using a battery of outcome measures in healthy older females. To determine whether self-reported functional losses are similar to those measured objectively and which best represent overall physical capacity. Design: Experimental study, cross-sectional analysis. Setting: Human Performance Laboratory, University setting. Participants: Thirty-nine community-dwelling women (mean [SD] age. =. 71.5 [7.3] years, range 60 to 83 years) completed a battery of objective measures of function and a self-reported health status survey. Main outcome measures: Objective measures: gait speed; TUG test; sit-to-stand; concentric knee flexor and extensor moments; self-reported: the SF-36. Results: Using a cross-sectional approach, annual declines were estimated for: TUG time (2.1%); gait speed (1.2%); knee extensor (2.2%) and flexor moments (3.0%); and self-reported Physical Functioning (0.9 to 1.2%) (p≤0.001). Linear regression indicated that age explained moderate variance in the objective (R2=21 to 34%) and self-reported (R2=14 to 28%) outcomes. TUG time and gait speed was significantly correlated with all objective outcomes except sit-to-stand (r=0.46 to 0.83) and most of the self-reported (r=0.40 to 0.63) outcomes (p<0.01). Conclusions: Age-related functional deterioration was estimated precisely across both objective and self-reported outcomes. Greater strength losses for the knee flexors compared to the extensors indicate an unequal strength loss of antagonistic muscle pairs which has implications for the safe completion of many functional tasks including obstacle negotiation, stair locomotion, postural transitions, and ultimately knee joint stability. Furthermore, walking speed and TUG time correlated most strongly with many of the outcomes highlighting their importance as global indicators of physical capacity

The EROS2 search for microlensing events towards the spiral arms: the complete seven season results

The EROS-2 project has been designed to search for microlensing events towards any dense stellar field. The densest parts of the Galactic spiral arms have been monitored to maximize the microlensing signal expected from the stars of the Galactic disk and bulge. 12.9 million stars have been monitored during 7 seasons towards 4 directions in the Galactic plane, away from the Galactic center. A total of 27 microlensing event candidates have been found. Estimates of the optical depths from the 22 best events are provided. A first order interpretation shows that simple Galactic models with a standard disk and an elongated bulge are in agreement with our observations. We find that the average microlensing optical depth towards the complete EROS-cataloged stars of the spiral arms is $\bar{\tau} =0.51\pm .13\times 10^{-6}$, a number that is stable when the selection criteria are moderately varied. As the EROS catalog is almost complete up to $I_C=18.5$, the optical depth estimated for the sub-sample of bright target stars with $I_C<18.5$ ($\bar{\tau}=0.39\pm >.11\times 10^{-6}$) is easier to interpret. The set of microlensing events that we have observed is consistent with a simple Galactic model. A more precise interpretation would require either a better knowledge of the distance distribution of the target stars, or a simulation based on a Galactic model. For this purpose, we define and discuss the concept of optical depth for a given catalog or for a limiting magnitude.Comment: 22 pages submitted to Astronomy & Astrophysic

Galactic Bulge Microlensing Events from the MACHO Collaboration

We present a catalog of 450 high signal-to-noise microlensing events observed by the MACHO collaboration between 1993 and 1999. The events are distributed throughout our fields and, as expected, they show clear concentration toward the Galactic center. No optical depth is given for this sample since no blending efficiency calculation has been performed, and we find evidence for substantial blending. In a companion paper we give optical depths for the sub-sample of events on clump giant source stars, where blending is not a significant effect. Several events with sources that may belong to the Sagittarius dwarf galaxy are identified. For these events even relatively low dispersion spectra could suffice to classify these events as either consistent with Sagittarius membership or as non-Sagittarius sources. Several unusual events, such as microlensing of periodic variable source stars, binary lens events, and an event showing extended source effects are identified. We also identify a number of contaminating background events as cataclysmic variable stars.Comment: 34 pages, 9 figures + 3 example lightcurves, all 564 lightcurves will be available at http://wwwmacho.mcmaster.ca, submitted to ApJ, see companion paper by Popowski et a

Collaborative futures:discursive realignments in austere times

This paper explores the future of collaboration in an era of austerity. Boundary object theory provides a framework to examine the significance and role of four key discourses in collaboration – efficiency, effectiveness, responsiveness and cultural performance. Crisis provides a way of examining how and in what ways discourses realign. The exploration of discourses aids critical analysis of collaboration across sectoral, geographical and disciplinary boundaries, highlighting the importance of understanding the contextual roots of collaboration theory and practice, and the implications of local/global dynamics

Milky Way demographics with the VVV survey III : Evidence for a great dark lane in the 157 million star bulge color-magnitude diagram

The new generation of IR surveys are revealing and quantifying Galactic features that provide an improved 3D interpretation of our own Galaxy. We present an analysis of the global distribution of dust clouds in the bulge using the near-IR photometry of 157 million stars from the VVV survey. We investigate the color-magnitude diagram of the Milky Way bulge, which shows a red giant clump of core He burning stars that is split into two color components, with a mean color difference of (Z-Ks) = 0.55 mag that is equivalent to AV = 2.0 mag. We conclude that there is an optically thick dust lane at intermediate latitudes above and below the plane that stretches across several square degrees from l =-10° to l = +10°. We call this feature the great dark lane. Although its exact distance is uncertain, it is located in front of the bulge. The evidence for a large-scale great dark lane within the Galactic bulge is important for constraining models of the barred Milky Way bulge and for comparing our galaxy with external barred galaxies in which these types of features are prominent. We discuss two other potential implications of the great dark lane for microlensing and bulge stellar populations studies.Peer reviewe

Horizontal Branch Stars: The Interplay between Observations and Theory, and Insights into the Formation of the Galaxy

We review HB stars in a broad astrophysical context, including both variable and non-variable stars. A reassessment of the Oosterhoff dichotomy is presented, which provides unprecedented detail regarding its origin and systematics. We show that the Oosterhoff dichotomy and the distribution of globular clusters (GCs) in the HB morphology-metallicity plane both exclude, with high statistical significance, the possibility that the Galactic halo may have formed from the accretion of dwarf galaxies resembling present-day Milky Way satellites such as Fornax, Sagittarius, and the LMC. A rediscussion of the second-parameter problem is presented. A technique is proposed to estimate the HB types of extragalactic GCs on the basis of integrated far-UV photometry. The relationship between the absolute V magnitude of the HB at the RR Lyrae level and metallicity, as obtained on the basis of trigonometric parallax measurements for the star RR Lyrae, is also revisited, giving a distance modulus to the LMC of (m-M)_0 = 18.44+/-0.11. RR Lyrae period change rates are studied. Finally, the conductive opacities used in evolutionary calculations of low-mass stars are investigated. [ABRIDGED]Comment: 56 pages, 22 figures. Invited review, to appear in Astrophysics and Space Scienc