New records of Holocene polar bear and walrus (Carnivora) in the Russian Arctic

This article discusses recent finds of Holocene polar bear and walrus from the northern regions of Russia. The ulna of a polar bear was found on Vaygach Island and radiocarbon dated to 1,971 +/- 25 BP (OxA-23631). This calibrates to 430-540 AD, taking into account the marine reservoir effect. The size of the bone is similar to that of a recent Ursus maritimus. The locality of the fossil bone is within the modern species range, which developed about two millennia ago. In 2014 a walrus tusk was found on the coast of New Siberia Island and is radiocarbon dated to 5,065 +/- 35 BP (GrA-62452). This calibrates to 3,510-3,370 BC, taking into account the marine reservoir effect. Its size and morphology are identical to that of an adult male of the subspecies Odobenus rosmarus laptevi. This subspecies populates the eastern parts of the Kara Sea, the entire Laptev Sea and the western parts of the East Siberian Sea. This new discovery could mean that populations of O. rosmarus laptevi inhabited the waters near the New Siberian Islands during the Middle Holocene, and that the present-day coastline of the Siberian Arctic Islands was already formed at that time

Multiproxy analysis of permafrost preserved faeces provides an unprecedented insight into the diets and habitats of extinct and extant megafauna

The study of faecal samples to reconstruct the diets and habitats of extinct megafauna has traditionally relied on pollen and macrofossil analysis. DNA metabarcoding has emerged as a valuable tool to complement and refine these proxies. While published studies have compared the results of these three proxies for sediments, this comparison is currently lacking for permafrost preserved mammal faeces. Moreover, most metabarcoding studies have focused on a single plant-specific DNA marker region. In this study, we target both the commonly used chloroplast trnL P6 loop as well as nuclear ribosomal ITS (nrITS). The latter can increase taxonomic resolution of plant identifications but requires DNA to be relatively well preserved because of the target length (∼300–500 bp). We compare DNA results to pollen and macrofossil analyses from permafrost and ice-preserved faeces of Pleistocene and Holocene megafauna. Samples include woolly mammoth, horse, steppe bison as well as Holocene and extant caribou. Most plant identifications were found using DNA, likely because the studied faeces contained many vegetative remains that could not be identified using macrofossils or pollen. Several taxa were, however, identified to lower taxonomic levels uniquely with macrofossil and pollen analysis. The nrITS marker provides species level taxonomic resolution for commonly encountered plant families that are hard to distinguish using the other proxies (e.g. Asteraceae, Cyperaceae and Poaceae). Integrating the results from all proxies, we are able to accurately reconstruct known diets and habitats of the extant caribou. Applying this approach to the extinct mammals, we find that the Holocene horse and steppe bison were not strict grazers but mixed feeders living in a marshy wetland environment. The mammoths showed highly varying diets from different non-analogous habitats. This confirms the presence of a mosaic of habitats in the Pleistocene ‘mammoth steppe’ that mammoths could fully exploit due to their flexibility in food choice

Grey wolf genomic history reveals a dual ancestry of dogs

The grey wolf (Canis lupus) was the first species to give rise to a domestic population, and they remained widespread throughout the last Ice Age when many other large mammal species went extinct. Little is known, however, about the history and possible extinction of past wolf populations or when and where the wolf progenitors of the present-day dog lineage (Canisfamiliaris) lived(1-8). Here we analysed 72 ancient wolf genomes spanning the last 100,000 years from Europe, Siberia and North America. We found that wolf populations were highly connected throughout the Late Pleistocene, with levels of differentiation an order of magnitude lower than they are today. This population connectivity allowed us to detect natural selection across the time series, including rapid fixation of mutations in the gene IFT8840,000-30,000 years ago. We show that dogs are overall more closely related to ancient wolves from eastern Eurasia than to those from western Eurasia, suggesting a domestication process in the east. However, we also found that dogs in the Near East and Africa derive up to half of their ancestry from a distinct population related to modern southwest Eurasian wolves, reflecting either an independent domestication process or admixture from local wolves. None of the analysed ancient wolf genomes is a direct match for either of these dog ancestries, meaning that the exact progenitor populations remain to be located.Peer reviewe

Comprehensive molecular characterization of gastric adenocarcinoma

Gastric cancer is a leading cause of cancer deaths, but analysis of its molecular and clinical characteristics has been complicated by histological and aetiological heterogeneity. Here we describe a comprehensive molecular evaluation of 295 primary gastric adenocarcinomas as part of The Cancer Genome Atlas (TCGA) project. We propose a molecular classification dividing gastric cancer into four subtypes: tumours positive for Epstein–Barr virus, which display recurrent PIK3CA mutations, extreme DNA hypermethylation, and amplification of JAK2, CD274 (also known as PD-L1) and PDCD1LG2 (also knownasPD-L2); microsatellite unstable tumours, which show elevated mutation rates, including mutations of genes encoding targetable oncogenic signalling proteins; genomically stable tumours, which are enriched for the diffuse histological variant and mutations of RHOA or fusions involving RHO-family GTPase-activating proteins; and tumours with chromosomal instability, which show marked aneuploidy and focal amplification of receptor tyrosine kinases. Identification of these subtypes provides a roadmap for patient stratification and trials of targeted therapies

Larix during the Mid-Pleniglacial (Greenland Interstadial 8) on Kotelny Island, northern Siberia

Needles, wood and pollen of Larix, recorded in a peat deposit from Kotelny Island, northern Siberia, indicate the local occurrence of larch around 38 000 cal. a BP, which is during the Middle Pleniglacial (Greenland Interstadial 8) of the Weichselian last glacial period. The pollen record, dominated by Cyperaceae, Poaceae, Betula, Alnus, Salix and Artemisia, indicates steppe‐tundra conditions with some shrubs and trees. The distribution of Larix species plays an important role in the reconstruction of climatic conditions during the Weichselian in Siberia. Nowadays Larix does not occur on Kotelny Island and in the present situation the temperature may not be the limiting factor for Larix, but instead the long, snowy winters and the moist tundra vegetation. Our data may contribute to a better understanding of Larix refugia and Weichselian climatic conditions in northern Siberia

New records of Holocene polar bear and walrus (Carnivora) in the Russian Arctic

This article discusses recent finds of Holocene polar bear and walrus from the northern regions of Russia. The ulna of a polar bear was found on Vaygach Island and radiocarbon dated to 1,971 +/- 25 BP (OxA-23631). This calibrates to 430-540 AD, taking into account the marine reservoir effect. The size of the bone is similar to that of a recent Ursus maritimus. The locality of the fossil bone is within the modern species range, which developed about two millennia ago. In 2014 a walrus tusk was found on the coast of New Siberia Island and is radiocarbon dated to 5,065 +/- 35 BP (GrA-62452). This calibrates to 3,510-3,370 BC, taking into account the marine reservoir effect. Its size and morphology are identical to that of an adult male of the subspecies Odobenus rosmarus laptevi. This subspecies populates the eastern parts of the Kara Sea, the entire Laptev Sea and the western parts of the East Siberian Sea. This new discovery could mean that populations of O. rosmarus laptevi inhabited the waters near the New Siberian Islands during the Middle Holocene, and that the present-day coastline of the Siberian Arctic Islands was already formed at that time

Sample characteristics of frozen mammals.

<p>Sample characteristics of frozen mammals.</p

Mammoth calf Lyuba had a hump on the neck consisting of special fat cavities.

<p>Mammoth calf Lyuba had a hump on the neck consisting of special fat cavities.</p

Approximate original fatty acid profiles calculated for frozen mammals.

<p>^a Only the major FAs have been considered.</p><p>^b A superscript number indicates the FA percentages found in frozen samples, while all derived percentages appear in italics. In reconstructed figures, C18 PUFA percentages are partially subtracted from PA figures; C20 PUFAs from EA. For both bison, reconstructed OA percentages are partially subtracted from PA.</p><p>^c PUFA ratios and PA percentage in agreement with those of grass-fed elephants <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084480#pone.0084480-Meyer1" target="_blank">[19]</a>.</p><p>^d PUFA ratios and PA percentage as grass-fed Siberian <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084480#pone.0084480-Mordovskaya1" target="_blank">[20]</a> and Galician horses <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084480#pone.0084480-GuilGuerrero1" target="_blank">[21]</a>.</p><p>^e PUFA ratios and PA percentage as grass-fed Bison subcutaneous fat <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084480#pone.0084480-Turner1" target="_blank">[22]</a>.</p

Unsaturated fatty acids composition of the fat from frozen mammals^a.

<p>^a Mean ± SD of three independent determinations performed by GLC-MS.</p