Differences and Similarities in TRAIL- and Tumor Necrosis Factor-Mediated Necroptotic Signaling in Cancer Cells.

Recently, a type of regulated necrosis (RN) called necroptosis was identified to be involved in many pathophysiological processes and emerged as an alternative method to eliminate cancer cells. However, only a few studies have elucidated components of TRAIL-mediated necroptosis useful for anticancer therapy. Therefore, we have compared this type of cell death to tumor necrosis factor (TNF)-mediated necroptosis and found similar signaling through acid and neutral sphingomyelinases, the mitochondrial serine protease HtrA2/Omi, Atg5, and vacuolar H(+)-ATPase. Notably, executive mechanisms of both TRAIL- and TNF-mediated necroptosis are independent of poly(ADP-ribose) polymerase 1 (PARP-1), and depletion of p38α increases the levels of both types of cell death. Moreover, we found differences in signaling between TNF- and TRAIL-mediated necroptosis, e.g., a lack of involvement of ubiquitin carboxyl hydrolase L1 (UCH-L1) and Atg16L1 in executive mechanisms of TRAIL-mediated necroptosis. Furthermore, we discovered indications of an altered involvement of mitochondrial components, since overexpression of the mitochondrial protein Bcl-2 protected Jurkat cells from TRAIL- and TNF-mediated necroptosis, and overexpression of Bcl-XL diminished only TRAIL-induced necroptosis in Colo357 cells. Furthermore, TRAIL does not require receptor internalization and endosome-lysosome acidification to mediate necroptosis. Taken together, pathways described for TRAIL-mediated necroptosis and differences from those for TNF-mediated necroptosis might be unique targets to increase or modify necroptotic signaling and eliminate tumor cells more specifically in future anticancer approaches

Quantum design of ionic liquids for extreme chemical inertness and a new theory of the glass transition

This is the accepted version of a paper subsequently published in the Journal of Solid State Electrochemistry. The final publication is available at Springer via http://dx.doi.org/10.1007/s10008-012-1974-2In many modern technologies (such as batteries and supercapacitors), there is a strong need for redox-stable ionic liquids. Experimentally, the stability of ionic liquids can be quantified by the voltage range over which electron tunneling does not occur, but so far, quantum theory has not been applied systematically to this problem. Here, we report the electrochemical reduction of a series of quaternary ammonium cations in the presence of bis(trifluoromethylsulfonyl)imide (TFSI) anions and use nonadiabatic electron transfer theory to explicate the results. We find that increasing the chain length of the alkyl groups confers improved chemical inertness at all accessible temperatures. Simultaneously, decreasing the symmetry of the quaternary ammonium cations lowers the melting points of the corresponding ionic liquids, in two cases yielding highly inert solvents at room temperature. These are called hexyltriethylammonium TFSI (HTE-TFSI) and butyltrimethylammonium TFSI (BTM-TFSI). Indeed, the latter are two of the most redox-stable solvents in the history of electrochemistry. To gain insight into their properties, very high precision electrical conductivity measurements have been carried out in the range +20 °C to +190 °C. In both cases, the data conform to the Vogel-Tammann-Fulcher (VTF) equation with “six nines” precision (R 2 > 0.999999). The critical temperature for the onset of conductivity coincides with the glass transition temperature T g. This is compelling evidence that ionic liquids are, in fact, softened glasses. Finally, by focusing on the previously unsuspected connection between the molecular degrees of freedom of ionic liquids and their bulk conductivities, we are able to propose a new theory of the glass transition. This should have utility far beyond ionic liquids, in areas as diverse as glassy metals and polymer science

L'Âge d'or de la Scala de Milan / Verdi, Tosti, Halevy... [et al.], comp. ; Victor Maurel, BAR ; Francesco Navarini, B ; Antonio Magini-Coletti, BAR... [et al.]

Collection : Bel cantoCollection : Bel cantoComprend : FALSTAFF. Quand'ero paggio / VERDI, comp. ; Victor MAUREL, BAR - NINON / Paolo TOSTI, comp. ; Victor MAUREL, BAR - LA JUIVE. Voi, che del Dio vivente / HALEVY, comp. ; Francesco NAVARINI, B - FALSTAFF. Duetto Alice-Falstaff / VERDI, comp. ; Antonio MAGINI-COLETTI, BAR ; Elisa PETRI, S - OTELLO. Ora e per sempre addio / VERDI, comp. ; Francesco VIGNAS, T - LA WALLY. Ebben ? ne andro lontana / CATALANI, comp. ; Teresa ARKEL, S - LUCREZIA BORGIA. Di pescator ignobile / DONIZETTI, comp. ; Eduardo GARBIN, T - DON SEBASTIANO. Terra adorata / G. DONIZETTI, comp. ; Ida RAPPINI, MS - LOHENGRIN. Di, non t'incanta / WAGNER, comp. ; Giuseppe BORGATTI, T - DESCRIPTION DE LONDRES / P. MASCAGNI, comp. ; Giuseppe PACINI, BAR - SERENATA A SURRIENTO / GAMBARDELLA, comp. ; Fernando de LUCIA, T - L'AFRICAINE. Air du sommeil / MEYERBEER, comp. ; Félia LITVINNE, S - ROMEO ET JULIETTE. Valse / GOUNOD, comp. ; Regina PINKERT, S - I PURITANI. A te, o cara / BELLINI, comp. ; Alessandro BONCI, T - ADRIANA LECOUVREUR. Io son l'umille ancella / CILEA, comp. ; Emma CARELLI, S - GUILLAUME TELL. Trio / ROSSINI, comp. ; Oreste LUPPI, B ; ESCALAIS, T ; MAGINI-COLETTI, BAR - LE ROI DE LAHORE. Arioso / MASSENET, comp. ; Eugenio GIRALDONI, BAR - LA PERLE DU BRESIL. Air du Mysoli / F. DAVID, comp. ; Regina PACINI, S - LA BOHEME. Mimi Pinson / LEONCAVALLO, comp. ; Rosina STORCHIO, S - OTELLO. Sipelciel / VERDI, comp. ; Giovanni ZENATELLO, T ; Pasquale AMATO, BAR - LUCREZIA BORGIA. Come é bello, quale incanto / DONIZETTI, comp. ; Maria de MACCHI, S - LES HUGUENOTS. Rondo d'Urbain / MEYERBEER, comp. ; Armanda PARSI-PETTINELLA, MS - LE BARBIER DE SEVILLE. Largo al factotum / ROSSINI, comp. ; Giuseppe de LUCA, BAR - LA FAVORITE. Spirto gentil / G. DONIZETTI, comp. ; Giuseppe ANSELMI, T - MIGNON. Io son Titania / A. THOMAS, comp. ; Maria BARRIENTOS, S - UN BALLO IN MASCHERA. Eri tu che macchiavi / VERDI, comp. ; Riccardo STRACCIARI, BAR - SIBERIA. Nel suo amore / GIORDANO, comp. ; Giannina RUSS, S - LE DEMON. Air / Anton RUBINSTEIN, comp. ; Mario SAMMARCO, BAR - LASCIALI DIR, TU M'AMI / QUARANTA, comp. ; Salomea KRUSCENITCHKA, S - LE FREISCHUTZ. Non nessun salvar ti puo / WEBER, comp. ; Adamo DIDUR, B - UN BALLO IN MASCHERA. Ma dall'arido stelo / VERDI, comp. ; Eugenia BURZIO, S - LES VEPRES SICILIENNES. Boléro / VERDI, comp. ; Ester MAZZOLENI, S - CANZONE GUERRESCA / GIORDANO, comp. ; Amedeo BASSI, T - MANON LESCAUT. Guardate ! pazzo, io son" / PUCCINI, comp. ; Giuseppe TACCANI, T - RIGOLETTO. Caro nome / VERDI, comp. ; Giuseppina FINZI-MAGRINI, S - ZAZA. E un riso gentil / LEONCAVALLO, comp. ; Emilio PEREA, T - DINORAH. Ombra leggera / MEYERBEER, comp. ; Elvira de HIDALGO, S - UN BALLO IN MASCHERA. La rivedro nell'estasi / VERDI, comp. ; Aureliano PERTILE, T - RIGOLETTO. Bella figlia dell'amore / VERDI, comp. ; Aureliano PERTILE, T ; MINGHINI-CATTANEO, MS ; BARDONE, S ; FREGOSI, BAR - MANON LESCAUT. In quelle trine morbide / PUCCINI, comp. ; Carmen MELIS, S - MIGNON. Elle est aimée / A. THOMAS, comp. ; Ninon VALLIN, S - LA TOSCA. Recondita armonia / PUCCINI, comp. ; Giacomo LAURI-VOLPI,T - FALSTAFF. L'onore ! ladri ! / VERDI, comp. ; Mariano STABILE, BAR - IRIS. La piovra / P. MASCAGNI, comp. ; Rosetta PAMPANINI, S - MIGNON. Duo des hirondelles / A. THOMAS, comp. ; Conchita SUPERVIA, MS ; BETTONI, B - ERNANI. Infelice ! e tu credevi / VERDI, comp. ; Tancredi PASERO, B - FRA DIAVOLO. Or son sola / VERDI, comp. ; Margherita CAROSIO, SBnF-Partenariats, Collection sonore - BelieveContient une table des matière