Tackling Fluid Overload in a High-transporter Diabetic Patient on Continuous Ambulatory Peritoneal Dialysis

Introduction: Diabetic patients on continuous ambulatory peritoneal dialysis (CAPD) are more prone to fluid overload than non-diabetic patients, but the use of hypertonic glucose solutions to improve their ultrafiltration (UF) may hamper their glycemic control. Maintaining euvolemia in such patients may be tricky and needs special care. Case report: A 72 year old diabetic and hypertensive patient presented with severe fluid overload shortly after initiation of CAPD despite producing more than one liter of urine per day. He only achieved modest ultrafiltration (UF) during the day, and had a negative UF during the long hypertonic night dwell. Peritoneal equilibration tests (PET) confirmed that he was a high transporter, and his weekly Kt/V was found to be 1.36. Since automated peritoneal dialysis (APD) is not yet available in Sudan, the PD prescription was modified to comprise five short cycles during the day, including two short hypertonic daytime dwells, and a dry abdomen at night. This approach succeeded in improving his fluid status, but required the addition of intra-peritoneal soluble insulin to his regular subcutaneous insulin in order to achieve acceptable blood sugar control. After 8 months his residual renal function (RRF) had declined remarkably and he began to suffer from intermittent fluid overload of variable degrees. Nevertheless, we managed to maintain him satisfactorily on CAPD for 14 months. Conclusion: Simple measures such as omitting the night dwell and using five short cycles during the day, including two short hypertonic dwells, can be effective in controlling fluid overload in diabetic patients who have a high transporter status. Key words: CAPD, diabetes mellitus, fluid overload, residual renal Functio

Assessment of the Immunogenic Potential of the Intermediate Infectious Bursal Disease Vaccine Virus (D 78) in Broiler Chicks

Abstract: The immunogenicity of the intermediate infectious bursal disease (IBD) vaccine virus (D-78), which commonly used in Sudan, was determined in broiler chicks in this study. The vaccine was employed via three routes of application namely aerosol, intranasal and drinking water. Both agar gel precipitation test (AGPT) and an indirect enzyme linked immunosorbent assay (ELISA) were used to detect the levels of antibody (Ab) responses in sera of vaccinated chicks. The results obtained showed that higher (P>0.05) levels of Abs were noted when the vaccine administered via aerosol route as compared to the intranasal and drinking water. The variation in the Ab levels among the chicks vaccinated with either the intranasal and drinking water using both tests was not significant ((P< 0.05). Following challenge of vaccinated chicks, the protection rates noted are correlated to the levels of Abs elicited. In conclusion, to achieve higher and protective immune status in chicks is recommended to apply the intermediate IBDV (D78) vaccine strain in broiler chicks via the aerosol route. AGPT can be used as a rapid qualitative test to determine the vaccine take among the chicks whereas ELISA should be used quantitatively to determine the levels of Ab responses in vaccinated chicks

Mitochondrial fusion is regulated by Reaper to modulate Drosophila programmed cell death

In most multicellular organisms, the decision to undergo programmed cell death in response to cellular damage or developmental cues is typically transmitted through mitochondria. It has been suggested that an exception is the apoptotic pathway of Drosophila melanogaster, in which the role of mitochondria remains unclear. Although IAP antagonists in Drosophila such as Reaper, Hid and Grim may induce cell death without mitochondrial membrane permeabilization, it is surprising that all three localize to mitochondria. Moreover, induction of Reaper and Hid appears to result in mitochondrial fragmentation during Drosophila cell death. Most importantly, disruption of mitochondrial fission can inhibit Reaper and Hid-induced cell death, suggesting that alterations in mitochondrial dynamics can modulate cell death in fly cells. We report here that Drosophila Reaper can induce mitochondrial fragmentation by binding to and inhibiting the pro-fusion protein MFN2 and its Drosophila counterpart dMFN/Marf. Our in vitro and in vivo analyses reveal that dMFN overexpression can inhibit cell death induced by Reaper or γ-irradiation. In addition, knockdown of dMFN causes a striking loss of adult wing tissue and significant apoptosis in the developing wing discs. Our findings are consistent with a growing body of work describing a role for mitochondrial fission and fusion machinery in the decision of cells to die

Persistence of frequency in gas–liquid flows across a change in pipe diameter or orientation

From a study of the characteristics of structures across a 67/38 mm sudden contraction, using air/silicone oil flows, it has been found that frequencies of the structures (mainly slugs) persist across the contraction. This is in contrast to the velocities and lengths which increase as they move into the smaller diameter pipe. These observations were found for both vertical and 5° upward orientations. A similar persistence of frequency has been found from four other sources in the literature: a vertical (gradual) contraction; a horizontal Venturi; and two cases of horizontal pipe, 90° bend and vertical riser combination. The latter were at two contrasting conditions: (i) at atmospheric pressure with air/water in small diameter (34 mm) pipes; (ii) at 20 bar in larger diameter pipes (189 mm) using nitrogen and naphtha

The control and maintenance of desired flow patterns in bends of different orientations

Multiphase flows are common in industrial settings and bends in pipe lines cannot be avoided due to space limitations. Gas-liquid two phase flows could form material discontinuities that could have adverse effect on productivity and the pipe network due to sudden variations resulting due to the rapid momentum flux variations at fittings such as bends. Research into gas-liquid flow and bends can be motivated by the effect of the bend on the flow downstream of it which could alter the flow pattern occurring and the performance of downstream equipment. Alternatively, the interest might come from what occurs in the bend itself, there could be dryout of the film on the walls and consequent damage to the heat transfer equipment. Here we present measurements made with a number of accurate and fast responding sensors on three cases, two on the effect of the bend and one considering effects in the bend. The results show that the flow transformations occur in two phase flows depending on the orientation of the bend and the change could be captured using fast sweeping measurement techniques. We present the evidence of effectiveness of several types of measurement techniques that could fit into various combinations of phases. The results, point to how to achieve certain flow patterns. Also recommendations are provided regarding the position of any sensor installed to determine flow pattern

Ebi/AP-1 Suppresses Pro-Apoptotic Genes Expression and Permits Long-Term Survival of Drosophila Sensory Neurons

Sensory organs are constantly exposed to physical and chemical stresses that collectively threaten the survival of sensory neurons. Failure to protect stressed neurons leads to age-related loss of neurons and sensory dysfunction in organs in which the supply of new sensory neurons is limited, such as the human auditory system. Transducin β-like protein 1 (TBL1) is a candidate gene for ocular albinism with late-onset sensorineural deafness, a form of X-linked age-related hearing loss. TBL1 encodes an evolutionarily conserved F-box–like and WD40 repeats–containing subunit of the nuclear receptor co-repressor/silencing mediator for retinoid and thyroid hormone receptor and other transcriptional co-repressor complexes. Here we report that a Drosophila homologue of TBL1, Ebi, is required for maintenance of photoreceptor neurons. Loss of ebi function caused late-onset neuronal apoptosis in the retina and increased sensitivity to oxidative stress. Ebi formed a complex with activator protein 1 (AP-1) and was required for repression of Drosophila pro-apoptotic and anti-apoptotic genes expression. These results suggest that Ebi/AP-1 suppresses basal transcription levels of apoptotic genes and thereby protects sensory neurons from degeneration

Epstein-Barr virus: clinical and epidemiological revisits and genetic basis of oncogenesis

Epstein-Barr virus (EBV) is classified as a member in the order herpesvirales, family herpesviridae, subfamily gammaherpesvirinae and the genus lymphocytovirus. The virus is an exclusively human pathogen and thus also termed as human herpesvirus 4 (HHV4). It was the first oncogenic virus recognized and has been incriminated in the causation of tumors of both lymphatic and epithelial nature. It was reported in some previous studies that 95% of the population worldwide are serologically positive to the virus. Clinically, EBV primary infection is almost silent, persisting as a life-long asymptomatic latent infection in B cells although it may be responsible for a transient clinical syndrome called infectious mononucleosis. Following reactivation of the virus from latency due to immunocompromised status, EBV was found to be associated with several tumors. EBV linked to oncogenesis as detected in lymphoid tumors such as Burkitt's lymphoma (BL), Hodgkin's disease (HD), post-transplant lymphoproliferative disorders (PTLD) and T-cell lymphomas (e.g. Peripheral T-cell lymphomas; PTCL and Anaplastic large cell lymphomas; ALCL). It is also linked to epithelial tumors such as nasopharyngeal carcinoma (NPC), gastric carcinomas and oral hairy leukoplakia (OHL). In vitro, EBV many studies have demonstrated its ability to transform B cells into lymphoblastoid cell lines (LCLs). Despite these malignancies showing different clinical and epidemiological patterns when studied, genetic studies have suggested that these EBV- associated transformations were characterized generally by low level of virus gene expression with only the latent virus proteins (LVPs) upregulated in both tumors and LCLs. In this review, we summarize some clinical and epidemiological features of EBV- associated tumors. We also discuss how EBV latent genes may lead to oncogenesis in the different clinical malignancie

Distance protection and fault location of the PV power plants distribution lines

Unlike the synchronous generators, the short-circuit current provided by the photovoltaic (PV) sources is limited by the grid-connected inverters and closely related to the normal conditions, which impacts the protection of the power systems. This study deals with the protection of the power lines (distribution feeders) that connect the PV power plants (PVPP) to the grid; the first part of this study analyses the impact of the grid-connected PV (GCPV) system on the conventional distance protection. A specific coordination between the over-current protection of the grid side and the distance protection of the PVPP side was used to eliminate this impact. This coordination requires delaying the distance protection and keeping the inverter connected to the grid, which was achieved by fault ride-through (FRT) feature accompanied with the inverter control during the fault condition. The second part studies the possibility of using the double-end-impedance-based fault location on the PVPP power lines, and some FRT strategies were suggested to reduce the effect of the inverter control loops on obtaining the accurate fault location. The proposed work was verified by GCPV system model using MATLAB/Simulink software