VARIAÇÃO DOS CONSTITUINTES BIOQUÍMICOS SANGUÍNEOS DE TARTARUGAS-DA-AMAZÔNIA (Podocnemis expansa, Schweigger 1812) (TESTUDINATA) MANTIDAS EM CRIATÓRIO COMERCIAL

The aim of the present research was to evaluate the biochemistry of blood constituents from the South American River Turtle Podocnemis expansa, Schweigger 1912. For this experiment, 46 animals from Moenda do Lago Farm, a commercial farm from São José dos Bandeirantes, Goiás State, Brazil, were used, the blood being collected from the vertebral sinus, trsnferred to vacuntainer tubes without anticoagulant, spun down, the serum collected in eppendorfs and storaged at 20C and the respective analysis carried out by colorimetric techniques and fast glucose sensor Precision Plus MedsenseÅ at the College of Veterinary Medicine Latobatories at Federal University of Uberlandia, Minas Gerais State, Brazil. The mean values found were as follows: Total proteins, 4.42 ± 0.55 g/dL, albumin, 2.50 ±0.2 g/dL, globulins, 1.92 ± 0.35 mg/dL, albumin/globulin ratio (A/G) 1.55 ±1.46, glucose, 122.9 ± 35.2 mg/dL, urea, 61.13 ± 28.35 mg/dL, creatinine, 0.098 ± 0.021 mg/dL, uric acid, 2.08 ± 0.53 mg/dL, cholesterol, 106.93 ± 21.03 mg/dL, total calcium,7.08 ± 1.04 mg/dL, ionic calcium, 4.41 ± 0.60 mg/dL, phosphorus, 5.21 ± 1.22 mg/dL, calcium/phosphorus ratio, 1.58 mg/dL ± 0.66, iron, 390.35 ± 116,65 µg/dL, magnesium, 1.59 ± 0.14, chlorides, 86.40 ± 7.63 mEq/ L, aspartate aminotransferase (AST) 194.56 ± 154.27 U/L, alanineaminotransferase (ALT) 4.04 ± 3.00 U/L, alkaline phosphatase, 131.13 ± 59.96 U/L, ¡- glutamiltransferase (GGT) 0.60 ± 0.84 U/L and tryglicerides, 127.65 ± 100.86 mg/dL. In conclusion, mean values of blood constituents of Podocnemis expansa did not show great differences from other chelonian species. The albumin/globulin ratio, and uric acid and glucose values were inversely related to body weight. Thus, the results of this research bring data on South America River Turtle blood biochemistry raised in captivity, although new research studies will be necessary to establish the species reference values.O objetivo desta pesquisa foi avaliar as variações dos constituintes bioquímicos sanguíneos da tartaruga Podocnemis expansa de um criatório comercial. Para isto, foram colhidas, por venipuntura do seio vertebral caudal, amostras de sangue de 46 animais mantidos em tanques na Fazenda Moenda do Lago, distrito de São José dos Bandeirantes, município de Nova Crixás GO. Após colhidas, as amostras de sangue foram transferidas rapidamente para frascos vacutainer® sem anticoagulante e levados à centrífuga para obtenção do soro, logo após transferidos para eppendorf e armazenados a 200C. O material foi transportado para o laboratório da Faculdade de Medicina Veterinária (FAMEV) da Universidade Federal de Uberlândia (UFU) para o seu processamento. As análises foram processadas colorimetricamente e em sensor rápido de glicose (Precision Plus Medsense®), os valores médios foram: proteínas totais 4,42±0,55 g/ dL, albumina 2,50±0,2 g/dL, globulinas 1,92±0,35 g/dL, relação albumina/globulinas (A/G) 1,55±1,46, glicose 122,9±35,2 mg/dL, uréia 61,13±28,35 mg/dL, creatinina 0,098±0,021 mg/dL, ácido úrico 2,08±0,53 mg/dL, cálcio total 7,08±1,04 mg/dL, cálcio ionizado 4,41±0,60 mg/ dL, colesterol 106,93±21,03 mg/dL, fósforo 5,21±1,22 mg/dL, relação cálcio/fósforo (Ca++/P) 1,58±0,66, ferro 390,35±116,65 ì g/dL, magnésio 1,59±0,14 mg/dL, cloretos 86,40±7,63 mEq/L, aspartato aminotransferase (AST) 194,56±154,27 U/L, alanino aminotransferase (ALT) 4,04±3,00 U/L, fosfatase alcalina 131,13±59,96 U/L, gama glutamiltransferase (GGT) 0,60±0,84 U/L e triglicérides 127,65±100,86 mg/dL. Conclui-se que os valores apresentam grandes diferenças dos valores de outras espécies de quelônios e que a relação albumina:globulina, as concentrações plasmáticas de ácido úrico e glicose são inversamente proporcionais às massas corporais dos animais. A espécie estudada apresenta escassos valores de bioquímica sanguínea na literatura, desta maneira os dados que foram apresentados auxiliam no estabelecimento de um padrão de bioquímica sanguínea para Podocnemis expansa criadas em cativeiro, mas também pode ser base para novos estudos

ORIGEM E RAMIFICAÇÕES DAS ARTÉRIAS MESENTÉRICAS CRANIAL E CAUDAL EM TARTARUGA DA AMAZÔNIA Podocnemis expansa Schweigger, 1812, (Testudinata-pelomedusidae)

Foram estudadas cinco Podocnemis expansa, fêmeas, cujo peso variava de 550 a 850 g. Os animais tiveram seu sistema arterial injetado com solução corada de látex sintético, fixados e armazenados em solução aquosa de formaldeído a 10%. Após abertura da cavidade celomática, as artérias mesentéricas cranial e caudal foram dissecadas. Encontrou-se a artéria mesentérica cranial como um ramo do tronco celíaco-mesentérico e originando as artérias pancreaticoduodenal caudal, ileocólica e jejunais, que irrigam o duodeno, pâncreas, cólon, íleo e jejuno, respectivamente. A artéria mesentérica caudal origina-se da aorta (60%) ou da artéria ilíaca comum (40%), distribuindo-se para o cólon e reto. Origin and ramifications of the cranial and caudal mesenteric arteries in Amazonian turtle - Podocnemis expansa Schweigger, 1812, Testudinata-Pelomedusidae Abstract An anatomic study has been carried out on the mesenteric arteries of five Podocnemis expansa females weighing from 550 to 680 g. The animals had their artery system injected with a latex solution colored with a specific pigment and were then fixed in a 10% formol solution. After the coelomatic cavity being opened the cranial and caudal mesenteric arteries were dissected. It has been found that the cranial mesenteric artery is a branch of the coeliac mesenteric trunk while the cranial mesenteric artery gives off the pancreatic-duodenal , ileocolic and jejunal arteries that irrigate the duodenum, pancreas, colon, ileum and jejunum respectively. The aorta gives origen of as much as 60% of the caudal mesenteric artery, the remaining 40 % being originated from the common ileac artery, their branches reaching the colon and the rectum

Search for single top quarks in the tau+jets channel using 4.8 fb−1^{-1} of ppˉp\bar{p} collision data

We present the first direct search for single top quark production using tau leptons. The search is based on 4.8 fb$^{-1}$ of integrated luminosity collected in $p\bar{p}$ collisions at $\sqrt{s}$=1.96 TeV with the D0 detector at the Fermilab Tevatron Collider. We select events with a final state including an isolated tau lepton, missing transverse energy, two or three jets, one or two of them $b$ tagged. We use a multivariate technique to discriminate signal from background. The number of events observed in data in this final state is consistent with the signal plus background expectation. We set in the tau+jets channel an upper limit on the single top quark cross section of \TauLimObs pb at the 95% C.L. This measurement allows a gain of 4% in expected sensitivity for the observation of single top production when combining it with electron+jets and muon+jets channels already published by the D0 collaboration with 2.3 fb$^{-1}$ of data. We measure a combined cross section of \SuperCombineXSall pb, which is the most precise measurement to date.Comment: 12 pages, 5 figure

Measurement of Z/gamma*+jet+X angular distributions in ppbar collisions at sqrt{s}=1.96 TeV

We present the first measurements at a hadron collider of differential cross sections for Z+jet+X production in delta phi(Z, jet), |delta y(Z, jet)| and |y_boost(Z, jet)|. Vector boson production in association with jets is an excellent probe of QCD and constitutes the main background to many small cross section processes, such as associated Higgs production. These measurements are crucial tests of the predictions of perturbative QCD and current event generators, which have varied success in describing the data. Using these measurements as inputs in tuning event generators will increase the experimental sensitivity to rare signals.Comment: Published in Physics Letters B 682 (2010), pp. 370-380. 15 pages, 6 figure

Search for the standard model Higgs boson in tau final states

We present a search for the standard model Higgs boson using hadronically decaying tau leptons, in 1 inverse femtobarn of data collected with the D0 detector at the Fermilab Tevatron ppbar collider. We select two final states: tau plus missing transverse energy and b jets, and tau+ tau- plus jets. These final states are sensitive to a combination of associated W/Z boson plus Higgs boson, vector boson fusion and gluon-gluon fusion production processes. The observed ratio of the combined limit on the Higgs production cross section at the 95% C.L. to the standard model expectation is 29 for a Higgs boson mass of 115 GeV.Comment: publication versio

Search for W' bosons decaying to an electron and a neutrino with the D0 detector

This Letter describes the search for a new heavy charged gauge boson W' decaying into an electron and a neutrino. The data were collected with the D0 detector at the Fermilab Tevatron proton-antiproton Collider at a center-of-mass energy of 1.96 TeV, and correspond to an integrated luminosity of about 1 inverse femtobarn. Lacking any significant excess in the data in comparison with known processes, an upper limit is set on the production cross section times branching fraction, and a W' boson with mass below 1.00 TeV can be excluded at the 95% C.L., assuming standard-model-like couplings to fermions. This result significantly improves upon previous limits, and is the most stringent to date.Comment: submitted to Phys. Rev. Let

Search for a scalar or vector particle decaying into Zgamma in ppbar collisions at sqrt(s) = 1.96 TeV

We present a search for a narrow scalar or vector resonance decaying into Zgamma with a subsequent Z decay into a pair of electrons or muons. The data for this search were collected with the D0 detector at the Fermilab Tevatron ppbar collider at a center of mass energy sqrt(s) = 1.96 TeV. Using 1.1 (1.0) fb-1 of data, we observe 49 (50) candidate events in the electron (muon) channel, in good agreement with the standard model prediction. From the combination of both channels, we derive 95% C.L. upper limits on the cross section times branching fraction (sigma x B) into Zgamma. These limits range from 0.19 (0.20) pb for a scalar (vector) resonance mass of 600 GeV/c^2 to 2.5 (3.1) pb for a mass of 140 GeV/c^2.Comment: Published by Phys. Lett.

Measurement of the ratios of the Z/G* + >= n jet production cross sections to the total inclusive Z/G* cross section in ppbar collisions at sqrt(s) = 1.96 TeV

We present a study of events with Z bosons and jets produced at the Fermilab Tevatron Collider in ppbar collisions at a center of mass energy of 1.96 TeV. The data sample consists of nearly 14,000 Z/G* -> e+e- candidates corresponding to the integrated luminosity of 0.4 fb-1 collected using the D0 detector. Ratios of the Z/G* + >= n jet cross sections to the total inclusive Z/G* cross section have been measured for n = 1 to 4 jet events. Our measurements are found to be in good agreement with a next-to-leading order QCD calculation and with a tree-level QCD prediction with parton shower simulation and hadronization.Comment: 7 pages, 2 figures, slightly modified, submitted to Phys. Lett.

Search for the associated production of a b quark and a neutral supersymmetric Higgs boson which decays to tau pairs

We report results from a search for production of a neutral Higgs boson in association with a $b$ quark. We search for Higgs decays to $\tau$ pairs with one $\tau$ subsequently decaying to a muon and the other to hadrons. The data correspond to 2.7fb$^{-1}$ of \ppbar collisions recorded by the D0 detector at $\sqrt{s} = 1.96$TeV. The data are found to be consistent with background predictions. The result allows us to exclude a significant region of parameter space of the minimal supersymmetric model.Comment: Submitted to Phys. Rev. Letter