118 research outputs found

    Dinámica espacial del mero Epinephelus morio (Pisces: Serranidae) en el Banco de Campeche, Golfo de México

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    The spatial dynamics of the red grouper Epinephelus morio plays a fundamental role in the fishery management on the Campeche Bank, where it is overfished. The objective of this study was to characterize the spatial dynamics of this population. We used a dynamic model of spatial distribution to estimate population movement rates between adjacent areas. The model was applied using information on catch per unit effort and length-frequency distributions for the Mexican mid-sized fleet that operated in the area in the period 1973-1977. These data were chosen to ensure that the information would reflect the condition of the resource before overfishing. It was possible to quantify the spatial dynamics of the red grouper and the results were consistent with previously known movement patterns. The results showed different movement rates and temporal patterns of movement for different population sectors. The spawning aggregations and the seasonal upwelling occurring in the eastern part of the Bank were considered as key factors influencing the pattern of population movements.La población del mero rojo Epinephelus morio del Banco de Campeche está sobreexplotada. La dinámica espacial del mero juega un papel fundamental en la gestión de la pesquería. El objetivo de este estudio fue caracterizar la dinámica espacial de la población del mero en el Banco de Campeche. Se utilizó un modelo dinámico de distribución espacial para estimar tasas de movimiento poblacional entre áreas adyacentes. El modelo se aplicó con información de las capturas por unidad de esfuerzo y de distribuciones de frecuencias de longitud provenientes de la flota mediana mexicana que operó en la zona de 1973 a 1977. Estos datos fueron elegidos para asegurar que la información reflejara el estado del recurso antes de la sobrepesca. Fue posible cuantificar la dinámica espacial del mero y los resultados concordaron con patrones de movimientos conocidos anteriormente. Los resultados mostraron distintos patrones temporales de movimiento para los diferentes sectores de población. Se considera que las agregaciones de desove y la surgencia estacional que ocurre en la parte oriental del Banco son factores clave que influyen en el patrón de los movimientos de población

    Desarrollo de competencias matemáticas en secundaria usando la técnica de aprendizaje orientado en proyectos

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    Este artículo presenta un estudio que tuvo por objetivo analizar el desarrollo de tres competencias matemáticas: planteamiento y resolución de problemas, comunicación y argumentación; con alumnos de segundo grado de secundaria, en una escuela pública mexicana, para identificar cómo impacta la técnica de aprendizaje orientada en proyectos (POL por sus siglas en inglés), como diseño instruccional innovador para mejorar los aprendizajes matemáticos de los estudiantes. La metodología utilizada fue el estudio de casos múltiples, aplicando instrumentos diversos tales como: el registro de bitácora, cuestionario, entrevista y análisis de proyectos aplicados a los estudiantes. Los hallazgos muestran el desarrollo de las tres competencias matemáticas al usar POL y resolver problemas de su interés y contexto, poniendo en juego un conjunto de capacidades como identificar, plantear y resolver problemas; explicando, justificando y demostrand

    Analysis of Key Features of Non-Linear Behavior Using Recurrence Plots. Case Study: Urban Pollution at Mexico City

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    ABSTRACT The use of Recurrence plots have been extensively used in various fields. In this work, Recurrence Plots (RPs) investigates the changes in the non-linear behaviour of urban air pollution using large datasets of raw data (hourly). This analysis has not been used before to extract information from large datasets for this type non-linear problem. Two different approaches have been used to tackle this problem. The first approach is to show results according to monitoring network. The second approach is to show the results by particle type. This analysis shows the feasibility of using Recurrence Analysis for pollution monitoring and control

    Effects of climate and land-use changes on fish catches across lakes at a global scale

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    Globally, our knowledge on lake fisheries is still limited despite their importance to food security and livelihoods. Here we show that fish catches can respond either positively or negatively to climate and land-use changes, by analyzing time-series data (1970–2014) for 31 lakes across five continents. We find that effects of a climate or land-use driver (e.g., air temperature) on lake environment could be relatively consistent in directions, but consequential changes in a lake-environmental factor (e.g., water temperature) could result in either increases or decreases in fish catch in a given lake. A subsequent correlation analysis indicates that reductions in fish catch was less likely to occur in response to potential climate and land-use changes if a lake is located in a region with greater access to clean water. This finding suggests that adequate investments for water-quality protection and water-use efficiency can provide additional benefits to lake fisheries and food security

    De novo assembly and transcriptome analysis of five major tissues of Jatropha curcas L. using GS FLX titanium platform of 454 pyrosequencing

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    <p>Abstract</p> <p>Background</p> <p><it>Jatropha curcas </it>L. is an important non-edible oilseed crop with promising future in biodiesel production. However, factors like oil yield, oil composition, toxic compounds in oil cake, pests and diseases limit its commercial potential. Well established genetic engineering methods using cloned genes could be used to address these limitations. Earlier, 10,983 unigenes from Sanger sequencing of ESTs, and 3,484 unique assembled transcripts from 454 pyrosequencing of uncloned cDNAs were reported. In order to expedite the process of gene discovery, we have undertaken 454 pyrosequencing of normalized cDNAs prepared from roots, mature leaves, flowers, developing seeds, and embryos of <it>J. curcas</it>.</p> <p>Results</p> <p>From 383,918 raw reads, we obtained 381,957 quality-filtered and trimmed reads that are suitable for the assembly of transcript sequences. <it>De novo </it>contig assembly of these reads generated 17,457 assembled transcripts (contigs) and 54,002 singletons. Average length of the assembled transcripts was 916 bp. About 30% of the transcripts were longer than 1000 bases, and the size of the longest transcript was 7,173 bases. BLASTX analysis revealed that 2,589 of these transcripts are full-length. The assembled transcripts were validated by RT-PCR analysis of 28 transcripts. The results showed that the transcripts were correctly assembled and represent actively expressed genes. KEGG pathway mapping showed that 2,320 transcripts are related to major biochemical pathways including the oil biosynthesis pathway. Overall, the current study reports 14,327 new assembled transcripts which included 2589 full-length transcripts and 27 transcripts that are directly involved in oil biosynthesis.</p> <p>Conclusion</p> <p>The large number of transcripts reported in the current study together with existing ESTs and transcript sequences will serve as an invaluable genetic resource for crop improvement in jatropha. Sequence information of those genes that are involved in oil biosynthesis could be used for metabolic engineering of jatropha to increase oil content, and to modify oil composition.</p

    Comparing de novo assemblers for 454 transcriptome data

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    <p>Abstract</p> <p>Background</p> <p>Roche 454 pyrosequencing has become a method of choice for generating transcriptome data from non-model organisms. Once the tens to hundreds of thousands of short (250-450 base) reads have been produced, it is important to correctly assemble these to estimate the sequence of all the transcripts. Most transcriptome assembly projects use only one program for assembling 454 pyrosequencing reads, but there is no evidence that the programs used to date are optimal. We have carried out a systematic comparison of five assemblers (CAP3, MIRA, Newbler, SeqMan and CLC) to establish best practices for transcriptome assemblies, using a new dataset from the parasitic nematode <it>Litomosoides sigmodontis</it>.</p> <p>Results</p> <p>Although no single assembler performed best on all our criteria, Newbler 2.5 gave longer contigs, better alignments to some reference sequences, and was fast and easy to use. SeqMan assemblies performed best on the criterion of recapitulating known transcripts, and had more novel sequence than the other assemblers, but generated an excess of small, redundant contigs. The remaining assemblers all performed almost as well, with the exception of Newbler 2.3 (the version currently used by most assembly projects), which generated assemblies that had significantly lower total length. As different assemblers use different underlying algorithms to generate contigs, we also explored merging of assemblies and found that the merged datasets not only aligned better to reference sequences than individual assemblies, but were also more consistent in the number and size of contigs.</p> <p>Conclusions</p> <p>Transcriptome assemblies are smaller than genome assemblies and thus should be more computationally tractable, but are often harder because individual contigs can have highly variable read coverage. Comparing single assemblers, Newbler 2.5 performed best on our trial data set, but other assemblers were closely comparable. Combining differently optimal assemblies from different programs however gave a more credible final product, and this strategy is recommended.</p
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