Compounds from fermented noni exudates (fNE) selectively kill human cancer cells

Our recent studies involving fermented noni exudates (fNE) made from noni (Morinda citrifolia) have shown promising anti-cancer activities. Using an in vitro cell culture system, this study examines dozens of compounds isolated from fNE in order to identify specific compounds preferentially toxic to tumor cells. The compounds at concentrations of 20, 5, or 2 μg/ml were added to NL-20 non-tumor lung cells or A549 lung carcinoma cells and incubated for 48 hours. The cytotoxicity of the compounds on the cells was measured using the MTS cell proliferation assay. Several compounds were found selectively effective in killing more A549 tumor cells than NL-20 cells. An antioxidant assay was then performed to investigate if a correlation exists between antioxidant activity and selective cancer cell killing in our samples. We found that samples BGS, C2, BGL, and C1 had the highest amount of antioxidant activity. Of these samples, only C2 showed significant selective A549 cell killing (p\u3c.05 at 2μg/ml). Further testing is necessary to determine the mechanism of action and its possible role in immune activation

The Darwinian shortfall in plants : phylogenetic knowledge is driven by range size

The Darwinian shortfall, i.e. the lack of knowledge of phylogenetic relationships, significantly impedes our understanding of evolutionary drivers of global patterns of biodiversity. Spatial bias in the Darwinian shortfall, where phylogenetic knowledge in some regions is more complete than others, could undermine eco- and biogeographic inferences. Yet, spatial biases in phylogenetic knowledge for major groups – such as plants – remain poorly understood. Using data for 337 023 species (99.7%) of seed plants (Spermatophyta), we produced a global map of phylogenetic knowledge based on regional data and tested several potential drivers of the observed spatial variation. Regional phylogenetic knowledge was defined as the proportion of the regional seed plant flora represented in GenBank's nucleotide database with phylogenetically relevant data. We used simultaneous autoregressive models to explain variation in phylogenetic knowledge based on three biodiversity variables (species richness, range size and endemism) and six socioeconomic variables representing funding and accessibility. We compared observed patterns and relationships to established patterns of the Wallacean shortfall (the lack of knowledge of species distributions). We found that the Darwinian shortfall is strongly and significantly related to the macroecological distribution of species' range sizes. Small-ranged species were significantly less likely to have phylogenetic data, leading to a concentration of the Darwinian shortfall in species-rich, tropical countries where range sizes are small on average. Socioeconomic factors were less important, with significant but quantitatively small effects of accessibility and funding. In conclusion, reducing the Darwinian shortfall and smoothen its spatial bias will require increased efforts to sequence the world's small-ranged (endemic) species

Early molecular layer interneuron hyperactivity triggers Purkinje neuron degeneration in SCA1.

Toxic proteinaceous deposits and alterations in excitability and activity levels characterize vulnerable neuronal populations in neurodegenerative diseases. Using in vivo two-photon imaging in behaving spinocerebellar ataxia type 1 (Sca1) mice, wherein Purkinje neurons (PNs) degenerate, we identify an inhibitory circuit element (molecular layer interneurons [MLINs]) that becomes prematurely hyperexcitable, compromising sensorimotor signals in the cerebellum at early stages. Mutant MLINs express abnormally elevated parvalbumin, harbor high excitatory-to-inhibitory synaptic density, and display more numerous synaptic connections on PNs, indicating an excitation/inhibition imbalance. Chemogenetic inhibition of hyperexcitable MLINs normalizes parvalbumin expression and restores calcium signaling in Sca1 PNs. Chronic inhibition of mutant MLINs delayed PN degeneration, reduced pathology, and ameliorated motor deficits in Sca1 mice. Conserved proteomic signature of Sca1 MLINs, shared with human SCA1 interneurons, involved the higher expression of FRRS1L, implicated in AMPA receptor trafficking. We thus propose that circuit-level deficits upstream of PNs are one of the main disease triggers in SCA1

An overview on the inconsistencies of approach in regulating the capital position of banks: Will the United Kingdom step out of line with Europe?

After the collapse of a number of banking institutions and bailouts of banks by governments, regulators have taken a different attitude and now appear keen to take regulation seriously when it comes to ensuring that banks have adequate capital and sufficient liquidity. Not only that, but in the United Kingdom, the Independent Commission on Banking Reform has made proposals with regard to the capital position of banks. This article, which is an overview, will look at matters from a UK perspective and at the proposals for reform. This article, after its introduction and summary, will look at a number of areas: first, the reforms made by Basel III; second, the regulation of Systemically Important Financial Institutions (Sifis) and the proposals for dealing with these; third, some matters in relation to lending that relate to capital and liquidity generally; fourth, increased stress testing of banks; fifth, derivatives and risk taking and the new proposed structure of regulation in the United Kingdom; sixth, the war of spin between regulators and banks; seventh, Shadow Banking; and eighth, The Independent Commission on Banking Reform and its proposals for reform. It will also be a theme that the various proposals lack consistency and that this could lead to regulatory arbitrage. It is already clear that there are inconsistencies between the various regulatory organisations, with proposals in the United Kingdom indicating that banks will be required to keep much higher levels of capital than those proposed by Basel and the European Community. The views of those who have pointed out inconsistencies between the United Kingdom and Basel/Europe have been highlighted

Hybrid imaging and timing ps laser excitation diagnostics for pulsed antihydrogen production

open48siIn this work we present a hybrid detection method providing simultaneous imaging and timing information suitable for fully monitoring positronium (Ps) formation, its laser excitation, and its spatial propagation for the first trials of pulsed antihydrogen (H) production through a charge-exchange reaction with trapped antiprotons (p). This combined method, based on the synchronous acquisition of an EJ-200 scintillation detector and a microchannel plate (MCP) detector with a dual readout (phosphor screen image and electrical pick-up signal), allows all relevant events in the experiment to be accurately determined in time while allowing high resolution images of e+ from Ps laser photodissociations to be acquired. The timing calibration process of the two detectors discussed in details as well as the future perspectives opened by this method.openCaravita R.; Antonello M.; Belov A.; Bonomi G.; Brusa R.S.; Caccia M.; Camper A.; Castelli F.; Comparat D.; Consolati G.; Demetrio A.; Di Noto L.; Doser M.; Fani M.; Ferragut R.; Gerber S.; Giammarchi M.; Gligorova A.; Gloggler L.T.; Guatieri F.; Haider S.; Hinterberger A.; Khalidova O.; Krasnicky D.; Lagomarsino V.; Malbrunot C.; Mariazzi S.; Matveev V.; Muller S.R.; Nebbia G.; Nedelec P.; Oberthaler M.; Oswald E.; Pagano D.; Penasa L.; Petracek V.; Prelz F.; Rienacker B.; Rohne O.M.; Rotondi A.; Sandaker H.; Santoro R.; Testera G.; Tietje I.; Toso V.; Wolz T.; Zimmer C.; Zurlo N.Caravita, R.; Antonello, M.; Belov, A.; Bonomi, G.; Brusa, R. S.; Caccia, M.; Camper, A.; Castelli, F.; Comparat, D.; Consolati, G.; Demetrio, A.; Di Noto, L.; Doser, M.; Fani, M.; Ferragut, R.; Gerber, S.; Giammarchi, M.; Gligorova, A.; Gloggler, L. T.; Guatieri, F.; Haider, S.; Hinterberger, A.; Khalidova, O.; Krasnicky, D.; Lagomarsino, V.; Malbrunot, C.; Mariazzi, S.; Matveev, V.; Muller, S. R.; Nebbia, G.; Nedelec, P.; Oberthaler, M.; Oswald, E.; Pagano, D.; Penasa, L.; Petracek, V.; Prelz, F.; Rienacker, B.; Rohne, O. M.; Rotondi, A.; Sandaker, H.; Santoro, R.; Testera, G.; Tietje, I.; Toso, V.; Wolz, T.; Zimmer, C.; Zurlo, N

Velocity-selected production of 2S3 metastable positronium

Positronium in the 2 3 S metastable state exhibits a low electrical polarizability and a long lifetime (1140 ns), making it a promising candidate for interferometry experiments with a neutral matter-antimatter system. In the present work, 2 3 S positronium is produced, in the absence of an electric field, via spontaneous radiative decay from the 3 3 P level populated with a 205-nm UV laser pulse. Thanks to the short temporal length of the pulse, 1.5 ns full width at half maximum, different velocity populations of a positronium cloud emitted from a nanochanneled positron-positronium converter were selected by delaying the excitation pulse with respect to the production instant. 2 3 S positronium atoms with velocity tuned between 7 7 10 4 ms 121 and 10 7 10 4 ms 121 were thus produced. Depending on the selected velocity, a 2 3 S production efficiency ranging from 3c0.8% to 3c1.7%, with respect to the total amount of emitted positronium, was obtained. The observed results give a branching ratio for the 3 3 P-2 3 S spontaneous decay of (9.7 \ub1 2.7)%. The present velocity selection technique could allow one to produce an almost monochromatic beam of 3c1 7 10 3 2 3 S atoms with a velocity spread of <10 4 ms 121 and an angular divergence of 3c50 mrad

Techniques for production and detection of 23S positronium

In this work, we show recent measurements of 23S long-lived positronium production via spontaneous decay from the 33P level. The possibility to tune the velocity of the 23S positronium, excited following this scheme, is presented. In the light of these results, we discuss the use of the 33P→23S transition to realize a monochromatic pulsed 23S positronium beam with low angular divergence. Preliminary tests of 23S beam production are presented. The possibility to overcome the natural 33P→23S branching ratio via stimulated emission, and thus increasing the intensity of the 23S source, is also shown. A position-sensitive detector for a pulsed beam of positronium, with spatial resolution of ≈ 90 μm, is finally described in view of its possible application for the spatial characterization of the 23S beam

The AEgIS experiment at CERN: Measuring antihydrogen free-fall in earth's gravitational field to test WEP with antimatter

The AEgIS (Antimatter Experiment: Gravity, Interferometry, Spectroscopy) experiment is designed with the objective to test the weak equivalence principle with antimatter by studying the free fall of antihydrogen in the Earth's gravitational field. A pulsed cold beam of antihydrogen will be produced by charge exchange between cold Ps excited in Rydberg state and cold antiprotons. Finally the free fall will be measured by a classical moir\ue9 deflectometer. The apparatus being assembled at the Antiproton Decelerator at CERN will be described, then the advancements of the experiment will be reported: positrons and antiprotons trapping measurements, Ps two-step excitation and a test-measurement of antiprotons deflection with a small scale moir\ue9 deflectometer