Uticaj transgenaze na kvalitet i prinos mesa kunića

In this paper results of the effect of transgenesis on quality and yield of rabbit meat are presented. During the trial body mass of transgenic progeny of F1 generation was monitored and compared to control group (nontransgenic animals of same age). Subsequent to slaughtering, meat yield, ratio between certain musculature parts and meat quality (proteins, lipids, water) were analyzed. Obtained data was compared to control group of animals of same age but standard genotype. Meat colour was evaluated on apparatus Specol 11 and expressed as percentage of remission on wave length of 540 μm. Content of elements in thigh muscle was established subsequent to dry mineralization in spectro-photometer UNICAM 939 Cambridge UK. Phosphorus content was measured spectro-photometrically on apparatus SPECOL 11. Subsequent to measuring and systematization, data was statistically analyzed and processed. Arithmetic mean values for certain groups of data were calculated, and their values compared using t-test (Hadživuković, 1991). Changes established in regard to content of water, lipids, energy and water binding capacity, were relative to changes in histological structure and level of metabolic processes. It is possible that these changes are result of pleiotropic effect of integrated gene. However, in order to confirm and interpret these changes, it is necessary to carry out further researches of the microscopic structure and metabolic processes of muscle tissues in transgenic rabbits.U radu su prikazani rezultati uticaja transgeneze na kvalitet i prinos mesa kunića. Ogled je vršen na komercijalnim tovnim hibridima nastalim ukrštanjem Novozelandskih belih i Kalifornijskih kunića. Dobijeni podaci upoređeni su sa kontrolnom grupom vršnjaka standardnog genotipa. Posmatrani su sledeći parametri kod obe grupe životinja: telesna masa (1, 2, 5, 10, 20 i 30- tog dana starosti), klanični podaci (w-težina pre žrtvovanja, dw- težina nakon iskrvarenja, s-težina kože, sp-težina distalnih delova zadnjih nogu, b- težina polutke sa kožom, hd-težina glave bez kože, fl- težina prednjih nogu, t- težina butova, r-težina rebara (grudi), bk - težina leđa, ht- težina srca, ky-težina bubrega, l- težina pluća, lvtežina jetre, git- težina stomaka i creva sa sadržajem, gite-težina praznog stomaka i creva, f- težina masnoće, bt- težina kostiju nogu, mt-težina mišića nogu, bf- težina kostiju prednjih nogu, c- prinos mesa (obraslost muskulaturom %); kvalitet mesa butova (cw- sadržaj vode, cp - sadržaj proteina, cf- sadržaj masti, ce- energija, pH, cc- boja, bw- kapacitet zadržavanja vode) i sadržaj mikroelemenata u mesu buta (Cu, Zn, Fe, K, Na, Mg, P, Ca). Posle merenja i sistematizacije podaci su statistički obrađeni. Izvršena su izračunavanja aritmetičkih sredina pojedinih grupa podataka, a zatim poređenje njihovih vrednosti t-testom (Hadživuković, 1991). Nizak nivo varijabiliteta u svim starosnim kategorijama u obe posmatrane grupe je jasno vidljiv iz vrednosti standardne greške aritmetičke sredine. To je manifestovano statistički značajnim uticajem procesa transgeneze na živu masu pri rođenju uprkos činjenici da je apsolutna razlika aritmetičkih sredina transgene i kontrolne grupe samo 0,005 kg (0,063±0,001 nasuprot 0,058±0,002) (tabela 1). Ovaj uticaj je na granici P=0,05 u tabeli analize varijanse (tabela 2). Razlika aritmetičkih sredina nije statistički značajna kod ostalih starosnih kategorija i ona se gubi već nakon 48 časova. Može se konstatovati da integrisani gen nema uticaja na porast transgenih kunića. Statistički značaj uticaja integrisanog gena je utvrđen kod parametara koji se odnose na masu distalnih delova transgenih i netransgenih kunića (0,062±0,001 nasuprot 0,069±0,001 kg), težine glave (0,119±0,003 nasuprot 0,128±0,003 kg) i težine butova (0,405±0,010 nasuprot 0,433±0,009 kg). Kod ostalih klaničnih karakteristika koje su testirane nisu utvrđene statistički značajne razlike koje bi bile uslovljene integracijom gena (tabele 3 i 4). Na uzorcima mesa nogu (butovi) praćene su vrednosti koje se odnose na kvalitet mesa (tabele 5 i 6). Dobijeni podaci koji se odnose na sastav mesa (tabela 5) ukazuju da je uticaj integracije gena bio statistički značajan (p (lt) 0,05) u grupi transgenih kunića u poređenju sa netransgenim u pogledu sledećih karakteristika: sadržaj proteina (74,03±0,26 nasuprot 74,84±0,28%), sadržaj masnoće (3,66±0,40 nasuprot 2,32±0,44%), sadržaja energije (495,43±11,81 nasuprot 458,07±12,94%), kapacitet zadržavanja vode (31,66±0,84 nasuprot 35,63±0,92%). Statistički značajne razlike kao posledica uticaja integrisanog gena nisu utvrđene kod ostalih posmatranih parametara (tabela 6). Srednje vrednosti sadržaja elemenata u tkivu mišića pokazale su najveće varijacije od svih posmatranih parametara. Najizraženije varijacije bile su u grupi netransgenih kunića (tabela 7). Broj životinja ili ponavljanja je igrao veliku ulogu. Za većinu posmatranih karakteristika može se reći da nisu pokazale uticaj integrisanog WAP- hFVIII gena u genotipu kunića. Značajnije razlike pojavile su se samo u okviru nekih parametara kvaliteta mesa (tabela 8). Rezultati ukazuju da nije utvrđeno ni prisustvo rhFVIII u skeletnim mišićima transgenih kunića

Ispitivanje biosinteze T-2 toksina u čistoj kulturi u različitim laboratorijskim uslovima

Biosinteza T-2 toksina u laboratorijskim uslovima je testirana kod sedam izolata Fusarium spp. Kultivacija je obavljena u pored o na prirodnom čvrstom supstratu (zrnu pšenice) i polusintetičkoj tečnoj podlozi (GPK). Gotovo sva proizvedena količina T-2 toksina je bila ekstracelularnog porekla. Veći prinosi ovog trihotecena su dobijeni kod izolata Fusarium kultivisanih na tečnoj podlozi: 0,8-175,0 mg/L filtra ta u odnosu na 12,5-52,5 mg/kg sintetisanih na zrnu pšenice. Fermentacija na tečnoj podlozi i rotacionoj mučkalici se pokazala kao pogodnija metoda za testiranje toksikogenosti gljivičnih izolata zbog kraće inkubacije, jednostavnije pripreme uzoraka i ekstrakcije T-2 toksina, dobijanja sirovih ekstrakata sa mnogo manje pratećih materija i mogućnosti preciznijeg definisanja faktora koji mogu da utiču na prinos istog trihotecena.Seven isolates of the fungal genus Fusarium, comprising three species: F.culmorum, F.sporotrichioides and F.tricinctum, were tested for T-2 toxin production. Cultivation was carried oout concurrently on a natural substrate (wheat grains) under temperature stress conditions (7 days at 28 °C, 21 days at 15-16 °C), and in a semi-synthetic liquid medium (5% glucose, 0.1 % peptone-1, 0.1 % yeast extract) on a rotary shaker (200 rpm, 5 days at 26 °C). Of the fungal isolates cultivated on wheat grains 85.7% were found to be toxicogenic; the yields of T-2 toxin were rather small (12.5-52.5 mg/kg dry weight). In the liquid medium all Fusarium cultures biosynthesized T-2 toxin; the quantities produced ranged from 0.8 to 175.0 mg/l of the filtrate. At least half of the dispensed sugar was present in the medium when T-2 toxin production started. No correlation was found between the type of mycelial growth, sporulation, biomass quantities and the intensity of T-2 toxin biosynthesis. Most of the produced trichothecene was of extracellular origin. Fermentation in the semi-synthetic liquid medium using a rotary shaker was more suitable for screening the toxicity of the fungal isolates in pure cultures because of a shorter period of incubation, simpler sample preparation and T-2 toxin extraction. In this way factors influencing toxin production could be defined more precisely

Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days

The aim of the present study was to investigate the influence of the protein content of seminal plasma on the motility, viability and acrosome integrity of spermatozoa in extended semen stored for 3 days. A total of 32 semen samples (from four boars) with high (4 mg/ml) and 32 semen samples (from four boars) with low (2 mg/ml) protein content were investigated. The semen samples were diluted by BTS at a ratio of 1:4, and stored for 72 h at 17oC. The percentages of live sperm (LS), live sperm with damaged acrosome (LDA) and total sperm with damaged acrosome (TDA) were detected by flow cytometry. Sperm progressive motility (PM) was detected using CASA. After 72 h of storage, the percentage of LS and PM was significantly (P lt 0.01) higher, and the LDA and TDA were significantly (P lt 0.01) lower in samples with high protein content than in the samples with low protein content (LS = 66 vs. 44%, PM = 64 vs. 48%, LDA = 15 vs. 21% and TDA = 29 vs. 45%, respectively). When comparing the difference between 0 and 72 h of storage, the percentage decrease in LS and PM, while increase in LDA and TDA were significantly higher in the samples with low (LS: 75 to 44%; PM: 68 to 48%; LDA: 11 to 21% and TDA: 23 to 45%) than in the samples with high protein content (LS: 78 to 66%; PM: 70 to 64%; LDA: 9 to 15% and TDA: 17 to 29%). We concluded that protein content in seminal plasma has a significant influence on progressive motility, viability and acrosome integrity in diluted semen stored for 3 days

Washing treatment impact on print quality of screen printed knitted fabrics

The surface of textile materials is highly textured, commonly in non-uniform ways. Because of this texture effect, textile surface appears rougher and more porous than other printing substrates, which can cause excessive ink penetration during printing process. Next, washing process is very important factor because it influences ink characteristics on printed samples as well as structural changes of the textile substrate. The aim of this paper is to determine the influences of washing process and different mesh tread count used for printing on print quality. This will be obtained by using spectrophotometric analysis, and GLCM image processing method for print mottle estimation. The results of this research show that increasing number of washing processes leads to higher color differences reproduction color in comparison to printed materials before washing. It also shows that textile surface texture has a great influence on print mottle as well as that number of washing treatment series can generate variations of solid-tone print uniformity. Keywords: cotton, different thread count, GLCM, spectrophotometric analysis, series of washing process

Molecular Identification and Characterisation of Aspergillus flavus Isolates Originating from Serbian Wheat Grains

During previous years. regarding the shifts in climate conditions in temperate region, such as occurrence of high temperatures and prolonged drought, increased occurrence frequencies of Aspergillus flavus and aflatoxins in cereal grains were recorded. A reliable and accurate identification of the fungi is of great importance for evaluating the microbiological risks of contamination. The essential point of the present investigation was molecular characterisation and identification of A. flavus isolates originating from common wheat and spelt grains collected after harvest during the period of three years (2015-2017) in Northern Serbia. A holistic approach that included PCR amplification of two DNA genomic regions and PCR-RFLP assay followed by fragment length analysis, provided complete and comprehensive characterisation of A. Amiss isolated from wheat grains. The presented results indicate that there was no difference among the tested A. spergillus isolates on the molecular-genetic level. All 38 strains were identified as A. flavus by sequencing of combined ITS region and beta-tubulin gene fragments (ace. no.: MH582473 to MH582510). PCR-RFLP method in combination with a Lab-on-a-chip (LoaC) electrophoresis can be successfully used to rapidly identify A. flavus isolates

In vitro uklanjanje mikotoksina korišćenjem različitih neorganskih adsorbenata i organskih otpadnih materijala iz Srbije

Aflatoxin B1 (AFB1), ochratoxin A (OTA), zearalenone (ZON), deoxynivalenol (DON) and T-2 toxin are the most extensively studied toxic fungal metabolites. Once mycotoxins enter the food/feed production chain keeping their toxic characteristics, it is very difficult to remove or eliminate them. One of promising methods to reduce mycotoxins in contaminated food/feedstuffs is the use of mycotoxin binders. This paper presents the results of in vitro investigations of mineral mycotoxin binders (bentonite - BEN, diatomite - DIA and zeolite - ZEO), and organic mycotoxin binders - agricultural waste materials (Myriophillium spicatum, peach and sour cherry pits). Chemical compositions of the adsorbents have showed that they do not consist of elements toxic to the animals. Inorganic adsorbents (BEN, DIA and ZEO) tested in vitro were better binders of AFB1 (94.97% - 96.90%), while the biosorbents were more efficient in adsorption of OTA (19.98% - 66.66%), ZON (33.33% - 75.00%) and T-2 toxin (16.67% - 50.00%). Inorganic adsorbents and organic waste materials expressed similar binding capacity for DON in vitro, with the exception of M. spicatum that did not at all adsorb this type B trichothecene. Our results indicate that feed contamination with different types of mycotoxins might be diminished by a product that combines different inorganic and organic adsorbents with diverse mycotoxin binding properties.Aflatoksin B1 (AFB1), ohratoksin A (OTA), zearalenon (ZON), dezoksinivalenol (DON) i T-2 toksin su najviše izučavani toksični metaboliti gljiva. Kada mikotoksini uđu u proizvodni lanac za hranu/hranu za životinje, zadržavajući svoje toksične karakteristike, teško ih je ukloniti ili eliminisati. Jedna od obećavajućih metoda za smanjenje nivoa mikotoksina u kontaminiranoj hrani/hrani za životinje je korišćenje mikotoksinskih veziva. Ovaj rad predstavlja rezultate in vitro istraživanja mineralnih mikotoksinskih veziva (bentonit - BEN, diatomit - DIA i zeolit - ZEO) i organskih veziva mikotoksina - poljoprivrednog otpadnog materijala (Myriophillium spicatum, koštice breskve i višnje). Hemijski sastavi adsorbenata pokazali su da ne sadrže elemente toksične za životinje. Neorganski adsorbenti (BEN, DIA i ZEO) testirani in vitro bolje su vezivali AFB1 (94,97% - 96,90%), dok su biosorbenti bili efikasniji u adsorpciji OTA (19,98% - 66,66%), ZON-a (33,33% - 75,00% ) i T-2 toksina (16,67% - 50,00%). Neorganski adsorbenti i organski otpadni materijali su pokazali sličan kapacitet in vitro vezivanja DON-a, sa izuzetkom M. spicatum koji uopšte nije adsorbovao ovaj trihotecen tipa B. Naši rezultati koji su prikazani ovde pokazuju da zagađivanje hrane i hrane za životinje različitim vrstama mikotoksina može biti smanjeno dodavanjem preparata dobijenog kombinacijom različitih neorganskih i organskih adsorbenata koji poseduju različite karakteristike vezivanja mikotoksina

Fractal and stereological analyses of insulin-induced rat exocrine pancreas remodelling

Background: The effect of insulin on the endocrine pancreas has been the subject of extensive study, but quantitative morphometric investigations of the exocrine pancreas are scarce. This study was therefore undertaken to investigate the effect of acute and chronic insulin administration (two doses, 0.4 IU and 4 IU) on the morphology of rat pancreas acini. Materials and methods: Semi-fine sections stained with methylene blue and basic fuchsine or haematoxylin and eosin-stained 5-micrometer thick paraffin sections were used for fractal and stereological analysis of exocrine acini. Acute insulin treatment, independent of applied doses increased fractal dimension in line with decreased lacunarity of pancreas acini. Chronic low dose insulin decreased fractal dimension and increased lacunarity of pancreas acini, but a high dose had the opposite effect. The volume densities (Vv) of cytoplasm, granules and nucleus are affected differently: acute low dose and high chronic dose significantly decreased granules Vv, and in line increased cytoplasmic Vv, whereas other examined structures showed slight changes without statistical significance. Results: The results obtained from this investigation indicate that insulin treatment induced structural remodelling of the exocrine pancreas suggesting a substantial role of insulin in its functioning. Conclusions: Additionally, we showed that fine architectural changes in acini could be detected by fractal analysis, suggesting this method as an alternative or addition to routine stereology

EUNIS Habitat Classification: Expert system, characteristic species combinations and distribution maps of European habitats

Aim: The EUNIS Habitat Classification is a widely used reference framework for European habitat types (habitats), but it lacks formal definitions of individual habitats that would enable their unequivocal identification. Our goal was to develop a tool for assigning vegetation‐plot records to the habitats of the EUNIS system, use it to classify a European vegetation‐plot database, and compile statistically‐derived characteristic species combinations and distribution maps for these habitats. Location: Europe. Methods: We developed the classification expert system EUNIS‐ESy, which contains definitions of individual EUNIS habitats based on their species composition and geographic location. Each habitat was formally defined as a formula in a computer language combining algebraic and set‐theoretic concepts with formal logical operators. We applied this expert system to classify 1,261,373 vegetation plots from the European Vegetation Archive (EVA) and other databases. Then we determined diagnostic, constant and dominant species for each habitat by calculating species‐to‐habitat fidelity and constancy (occurrence frequency) in the classified data set. Finally, we mapped the plot locations for each habitat. Results: Formal definitions were developed for 199 habitats at Level 3 of the EUNIS hierarchy, including 25 coastal, 18 wetland, 55 grassland, 43 shrubland, 46 forest and 12 man‐made habitats. The expert system classified 1,125,121 vegetation plots to these habitat groups and 73,188 to other habitats, while 63,064 plots remained unclassified or were classified to more than one habitat. Data on each habitat were summarized in factsheets containing habitat description, distribution map, corresponding syntaxa and characteristic species combination. Conclusions: EUNIS habitats were characterized for the first time in terms of their species composition and distribution, based on a classification of a European database of vegetation plots using the newly developed electronic expert system EUNIS‐ESy. The data provided and the expert system have considerable potential for future use in European nature conservation planning, monitoring and assessment

Classification of mesic grasslands and their transitions of South Transdanubia (Hungary)

Relevés from meadows and pastures of South Transdanubia (Hungary) are evaluated by clustering and ordination methods. The relevé selection focused on the Arrhenatheretalia order but its transitions towards other types were also included. The groups of relevés are delimited and described according to differential, dominant and constant species. Ecological conditions of the groups were compared using indicator values. Nine groups were distinguished, four of them belonging strictly to the order Arrhenatheretalia. Each alliance of Arrhenatheretalia presented in the study area (Cynosurion, Arrhenatherion) was represented by two groups. Groups from these two alliances are separated along a light gradient, while groups of the same alliance differ in nutrient values. Within Cynosurion, the nutrient-poor group cannot be identified unambiguously as any syntaxa previously known from Hungary. The nutrient-rich Cynosurion meadows are similar to Lolio–Cynosuretum, however, they show a stronger relationship with wet meadows. Within Arrhenatherion, Pastinaco–Arrhenatheretum is recognised as a hay meadow of nutrient-rich soils. The other meadow type is similar to Filipendulo–Arrhenatheretum, thus raising syntaxonomical problems. There are transitional groups towards semi-dry and wet meadows, one dynamic phase and one outlier group among the other five clusters