Fundamental parameters of 16 late-type stars derived from their angular diameter measured with VLTI/AMBER

Thanks to their large angular dimension and brightness, red giants and supergiants are privileged targets for optical long-baseline interferometers. Sixteen red giants and supergiants have been observed with the VLTI/AMBER facility over a two-years period, at medium spectral resolution (R=1500) in the K band. The limb-darkened angular diameters are derived from fits of stellar atmospheric models on the visibility and the triple product data. The angular diameters do not show any significant temporal variation, except for one target: TX Psc, which shows a variation of 4% using visibility data. For the eight targets previously measured by Long-Baseline Interferometry (LBI) in the same spectral range, the difference between our diameters and the literature values is less than 5%, except for TX Psc, which shows a difference of 11%. For the 8 other targets, the present angular diameters are the first measured from LBI. Angular diameters are then used to determine several fundamental stellar parameters, and to locate these targets in the Hertzsprung-Russell Diagram (HRD). Except for the enigmatic Tc-poor low-mass carbon star W Ori, the location of Tc-rich stars in the HRD matches remarkably well the thermally-pulsating AGB, as it is predicted by the stellar-evolution models. For pulsating stars with periods available, we compute the pulsation constant and locate the stars along the various sequences in the Period -- Luminosity diagram. We confirm the increase in mass along the pulsation sequences, as predicted by the theory, except for W Ori which, despite being less massive, appears to have a longer period than T Cet along the first-overtone sequence.Comment: 15 pages, 9 figures, 6 table

The binary fraction in the globular cluster M10 (NGC 6254): comparing core and outer regions

We study the binary fraction of the globular cluster M10 (NGC 6254) as a function of radius from the cluster core to the outskirts, by means of a quan- titative analysis of the color distribution of stars relative to the fiducial main sequence. By taking advantage of two data-sets, acquired with the Advanced Camera for Survey and the Wide Field Planetary Camera 2 on board the Hubble Space Telescope, we have studied both the core and the external regions of the cluster. The binary fraction is found to decrease from 14% within the core, to 1.5% in a region between 1 and 2 half-mass radii from the cluster centre. Such a trend and the derived values are in agreement with previous results ob- tained in clusters of comparable total magnitude. The estimated binary fraction is sufficient to account for the suppression of mass segregation observed in M10, without any need to invoke the presence of an intermediate-mass black hole in its centre.Comment: Accepted for publication in ApJ (22 pages, 7 figures, 3 tables

The extended halo of NGC 2682 (M 67) from Gaia DR2

Context: NGC 2682 is a nearby open cluster, approximately 3.5 Gyr old. Dynamically, most open clusters should dissolve on shorter timescales, of ~ 1 Gyr. Having survived until now, NGC 2682 was likely much more massive in the past, and is bound to have an interesting dynamical history. Aims: We investigate the spatial distribution of NGC 2682 stars to constrain its dynamical evolution, especially focusing on the marginally bound stars in the cluster outskirts. Methods: We use Gaia DR2 data to identify NGC 2682 members up to a distance of ~150 pc (10 degrees). Two methods (Clusterix and UPMASK) are applied to this end. We estimate distances to obtain three-dimensional stellar positions using a Bayesian approach to parallax inversion, with an appropriate prior for star clusters. We calculate the orbit of NGC 2682 using the GRAVPOT16 software. Results: The cluster extends up to 200 arcmin (50 pc) which implies that its size is at least twice as previously believed. This exceeds the cluster Hill sphere based on the Galactic potential at the distance of NGC 2682. Conclusions: The extra-tidal stars in NGC 2682 may originate from external perturbations such as disk shocking or dynamical evaporation from two-body relaxation. The former origin is plausible given the orbit of NGC 2682, which crossed the Galactic disk ~40 Myr ago.Comment: 9 pages, 5 figures, accepted for publication on A&

Stereoselective Solvolysis in the Synthesis of Dorzolamide Intermediates

The key intermediate in the synthesis of dorzolamide,(4S,6S)-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-4-ol-7,7-dioxide,can beobtained in the diastereoisomerically pure form in two straightforwardsteps starting from diastereoisomeric mixtures of cis/trans-(6S)-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-4-yl acetate,regardless of their ratio. The reaction of crucial importance in thisscheme is a remarkably stereoselective solvolysis of the acetate esterin an acetone/phosphate buffer mixture as the solvent system. Investigationof this so far unrecognized stereoselective reaction reveals thatit proceeds via an S(N)1-like pathway as indicated by thecorrelation of the solvolysis rate constants with the Y (OTs) values of different solvent mixtures and by trappingof the reaction intermediate with sodium azide. The structure of (4S,6S)-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-4-ol-7,7-dioxide was confirmedby single-crystal X-ray analysis

Constraining Intermediate-Mass Black Holes in Globular Clusters

Decades after the first predictions of intermediate-mass black holes (IMBHs) in globular clusters (GCs) there is still no unambiguous observational evidence for their existence. The most promising signatures for IMBHs are found in the cores of GCs, where the evidence now comes from the stellar velocity distribution, the surface density profile, and, for very deep observations, the mass-segregation profile near the cluster center. However, interpretation of the data, and, in particular, constraints on central IMBH masses, require the use of detailed cluster dynamical models. Here we present results from Monte Carlo cluster simulations of GCs that harbor IMBHs. As an example of application, we compare velocity dispersion, surface brightness and mass-segregation profiles with observations of the GC M10, and constrain the mass of a possible central IMBH in this cluster. We find that, although M10 does not seem to possess a cuspy surface density profile, the presence of an IMBH with a mass up to 0.75% of the total cluster mass, corresponding to about 600 Msun, cannot be excluded. This is also in agreement with the surface brightness profile, although we find it to be less constraining, as it is dominated by the light of giants, causing it to fluctuate significantly. We also find that the mass-segregation profile cannot be used to discriminate between models with and without IMBH. The reason is that M10 is not yet dynamically evolved enough for the quenching of mass segregation to take effect. Finally, detecting a velocity dispersion cusp in clusters with central densities as low as in M10 is extremely challenging, and has to rely on only 20-40 bright stars. It is only when stars with masses down to 0.3 Msun are included that the velocity cusp is sampled close enough to the IMBH for a significant increase above the core velocity dispersion to become detectable.Comment: 30 pages, 8 figures, submitted to Ap

Molecular evolution of the proopiomelanocortin system in Barn owl species.

Examination of genetic polymorphisms in outbred wild-living species provides insights into the evolution of complex systems. In higher vertebrates, the proopiomelanocortin (POMC) precursor gives rise to α-, β-, and γ-melanocyte-stimulating hormones (MSH), which are involved in numerous physiological aspects. Genetic defects in POMC are linked to metabolic disorders in humans and animals. In the present study, we undertook an evolutionary genetic approach complemented with biochemistry to investigate the functional consequences of genetic polymorphisms in the POMC system of free-living outbred barn owl species (family Tytonidae) at the molecular level. Our phylogenetic studies revealed a striking correlation between a loss-of-function H9P mutation in the β-MSH receptor-binding motif and an extension of a poly-serine stretch in γ3-MSH to ≥7 residues that arose in the barn owl group 6-8 MYA ago. We found that extension of the poly-serine stretches in the γ-MSH locus affects POMC precursor processing, increasing γ3-MSH production at the expense of γ2-MSH and resulting in an overall reduction of γ-MSH signaling, which may be part of a negative feedback mechanism. Extension of the γ3-MSH poly-serine stretches ≥7 further markedly increases peptide hormone stability in plasma, which is conserved in humans, and is likely relevant to its endocrine function. In sum, our phylogenetic analysis of POMC in wild living owls uncovered a H9P β-MSH mutation subsequent to serine extension in γ3-MSH to 7 residues, which was then followed by further serine extension. The linked MSH mutations highlight the genetic plasticity enabled by the modular design of the POMC gene

A Molecular Sensor To Characterize Arenavirus Envelope Glycoprotein Cleavage by Subtilisin Kexin Isozyme 1/Site 1 Protease.

UNLABELLED: Arenaviruses are emerging viruses including several causative agents of severe hemorrhagic fevers in humans. The advent of next-generation sequencing technology has greatly accelerated the discovery of novel arenavirus species. However, for many of these viruses, only genetic information is available, and their zoonotic disease potential remains unknown. During the arenavirus life cycle, processing of the viral envelope glycoprotein precursor (GPC) by the cellular subtilisin kexin isozyme 1 (SKI-1)/site 1 protease (S1P) is crucial for productive infection. The ability of newly emerging arenaviruses to hijack human SKI-1/S1P appears, therefore, to be a requirement for efficient zoonotic transmission and human disease potential. Here we implement a newly developed cell-based molecular sensor for SKI-1/S1P to characterize the processing of arenavirus GPC-derived target sequences by human SKI-1/S1P in a quantitative manner. We show that only nine amino acids flanking the putative cleavage site are necessary and sufficient to accurately recapitulate the efficiency and subcellular location of arenavirus GPC processing. In a proof of concept, our sensor correctly predicts efficient processing of the GPC of the newly emergent pathogenic Lujo virus by human SKI-1/S1P and defines the exact cleavage site. Lastly, we employed our sensor to show efficient GPC processing of a panel of pathogenic and nonpathogenic New World arenaviruses, suggesting that GPC cleavage represents no barrier for zoonotic transmission of these pathogens. Our SKI-1/S1P sensor thus represents a rapid and robust test system for assessment of the processing of putative cleavage sites derived from the GPCs of newly discovered arenavirus by the SKI-1/S1P of humans or any other species, based solely on sequence information. IMPORTANCE: Arenaviruses are important emerging human pathogens that can cause severe hemorrhagic fevers with high mortality in humans. A crucial step in productive arenavirus infection of human cells is the processing of the viral envelope glycoprotein by the cellular subtilisin kexin isozyme 1 (SKI-1)/site 1 protease (S1P). In order to break the species barrier during zoonotic transmission and cause severe disease in humans, newly emerging arenaviruses must be able to hijack human SKI-1/S1P efficiently. Here we implement a newly developed cell-based molecular sensor for human SKI-1/S1P to characterize the processing of arenavirus glycoproteins in a quantitative manner. We further use our sensor to correctly predict efficient processing of the glycoprotein of the newly emergent pathogenic Lujo virus by human SKI-1/S1P. Our sensor thus represents a rapid and robust test system with which to assess whether the glycoprotein of any newly emerging arenavirus can be efficiently processed by human SKI-1/S1P, based solely on sequence information

Mn-Containing Bioactive Glass-Ceramics: BMP-2-Mimetic Peptide Covalent Grafting Boosts Human-Osteoblast Proliferation and Mineral Deposition

The addition of Mn in bioceramic formulation is gaining interest in the field of bone implants. Mn activates human osteoblast (h-osteoblast) integrins, enhancing cell proliferation with a dose-dependent effect, whereas Mn-enriched glasses induce inhibition of Gram-negative or Gram-positive bacteria and fungi. In an effort to further optimize Mn-containing scaffolds' beneficial interaction with h-osteoblasts, a selective and specific covalent functionalization with a bioactive peptide was carried out. The anchoring of a peptide, mapped on the BMP-2 wrist epitope, to the scaffold was performed by a reaction between an aldehyde group of the peptide and the aminic groups of silanized Mn-containing bioceramic. SEM-EDX, FT-IR, and Raman studies confirmed the presence of the peptide grafted onto the scaffold. In in vitro assays, a significant improvement in h-osteoblast proliferation, gene expression, and calcium salt deposition after 7 days was detected in the functionalized Mn-containing bioceramic compared to the controls

Imaging simulations of selected science with the Magdalena Ridge Observatory Interferometer

We present simulated observations of surface features on Red Supergiant (RSG) stars and clumpy dust structures surrounding Active Galactic Nuclei (AGN) with the Magdalena Ridge Observatory Interferometer (MROI). These represent two of the classes of astrophysical targets enumerated in the MROI Key Science Mission that are typical of the types of complex astrophysical phenomena that the MROI has been designed to image. The simulations are based on source structures derived from recent theoretical models and include both random and systematic noise on the measured Fourier data (visibility amplitudes and closure phases) consistent with our expectations for typical such targets observed with the MROI. Image reconstructions, obtained using the BSMEM imaging package, are presented for 4-, 6- and 8- telescope implementations of the array. Although a rudimentary imaging capability is demonstrated with only 4 telescopes, the detailed features of targets are only reliably determined when at least 6 telescopes are present. By the tine 8 telescope are used, the reconstructed images are sufficiently faithful to allow the discrimination between competing models, confirming the design goal of the MROI, i.e. to offer model-independent near-infrared imaging on sub-milliarcsecond scales