Probiotic supplementation influences the diversity of the intestinal microbiota during early stages of farmed Senegalese sole (Solea senegalensis, Kaup, 1858)

Ingestion of bacteria at early stages results in establishment of a primary intestinal microbiota which likely undergoes several stages along fish life. The role of this intestinal microbiota regulating body functions is crucial for larval development. Probiotics have been proved to modulate this microbiota and exert antagonistic effects against fish pathogens. In the present study, we aimed to determine bacterial diversity along different developmental stages of farmed Senegalese sole (Solea senegalensis) after feeding probiotic (Shewanella putrefaciens Pdp11) supplemented diet for a short period (10–30 days after hatching, DAH). Intestinal lumen contents of sole larvae fed control and probiotic diets were collected at 23, 56, 87, and 119 DAH and DNA was amplified using 16S rDNA bacterial domain-specific primers. Amplicons obtained were separated by denaturing gradient gel electrophoresis (DGGE), cloned, and resulting sequences compared to sequences in GenBank. Results suggest that Shewanella putrefaciens Pdp11 induces a modulation of the dominant bacterial taxa of the intestinal microbiota from 23 DAH. DGGE patterns of larvae fed the probiotic diet showed a core of bands related to Lactobacillus helveticus, Pseudomonas acephalitica, Vibrio parahaemolyticus,and Shewanella genus, together with increased Vibri o genus presence. In addition, decreased number of clones related to Photobacterium damselae subsp piscicida at 23 and 56 DAH was observed in probiotic-fed larvae. A band corresponding to Shewanella putrefaciens Pdp11 was sequenced as predominant from 23 to 119 DAH samples, confirming the colonization by the probiotics. Microbiota modulation obtained via probiotics addition emerges as an effective tool to improve Solea senegalensis larviculture.En prens

The Effects of Increased Intensity and Number of Repetitions of Training on SJFT in Juniors and Cadets

This study aims to determine the effects of a program based on a progressive increase in the number of nage-komi repetitions (throws) on a specific judo fitness test (SJFT). The research was conducted on a total of 20 respondents. In SJFT for research purposes, 12 variables. Descriptive statistics and paired t-tests for dependent samples of initial and final testing were used for data processing. The results of the study showed significant statistical deviations in the number of throws in the first 15 seconds, with the pulse measured immediately after testing and the SJFT index (p <005). There is a noticeable need for a table to classify SJFT results for young judoists. Based on the obtained results, we can conclude that the programmed activity had positive effects on the results of the SJFT, ie that the index was statistically significantly lower in the final than in the initial testing. Taking into account the results obtained, calculated by the SPSS 22 t-test for dependent variables, the values ??of the young judoist index and the values ??of the senior index show noticeable differences. While their results can be classified into appropriate categories, the results of young judoists can be classifieclassifiedrity, under, ad and the rest as very bad. Some results could not even be ranked, so we conclude that it is necessary to make tables for classifying the results of young judoists. The obtained results can be used as guidelines for more efficient programming of the training process. Keywords: cadets, judo, juniors, SJFT test, training

Peptidomic analysis reveals proteolytic activity of kefir microorganisms on bovine milk proteins

SCOPE: The microorganisms that make up kefir grains are well known for lactose fermentation, but the extent to which they hydrolyze and consume milk proteins remains poorly understood. Peptidomics technologies were used to examine the proteolytic activity of kefir grains on bovine milk proteins. METHODS AND RESULTS: Gel electrophoresis revealed substantial digestion of milk proteins by kefir grains, with mass spectrometric analysis showing the release of 609 protein fragments and alteration of the abundance of >1,500 peptides that derived from 27 milk proteins. Kefir contained 25 peptides identified from the literature as having biological activity, including those with antihypertensive, antimicrobial, immunomodulatory, opioid and anti-oxidative functions. 16S rRNA and shotgun metagenomic sequencing identified the principle taxa in the culture as Lactobacillus species. CONCLUSION: The model kefir sample contained thousands of protein fragments released in part by kefir microorganisms and in part by native milk proteases

White cabbage (Brassica oleracea var. capitata f. alba) : botanical, phytochemical and pharmacological overview

White cabbage (Brassica oleraceae var. capitata f. alba) is a cruciferous vegetable used worldwide as a food and in traditional medicine. Due to its common availability in local markets, affordability, and consumer preference, it represents a significant source of phytonutrients in the human diet. This review provides an overview of white cabbage origin, taxonomy, geographical distribution, botanical characteristics, and contemporary and traditional uses, as well as its phytochemicals and pharmacology. Special emphasis is placed on a health-promoting phytochemicals such as glucosinolates, polyphenols, and vitamins, as well as anticancerogenic, antioxidant, anti- inflamantory and cardioprotective effects. The majority of so far published research on white cabbage was focused on qualitative determination of phytochemicals (targeted analysis), while only few recent papers published data based on untargeted metabolomic profiling. Hence, this review discusses and emphasizes a further need of studying the white cabbage phytochemicals using modern metabolomics platforms which will enable scientists to pinpoint the exact bioactive metabolites which are responsible for certain bioactivity