全反式视黄酸体外处理胃癌细胞对其裸小鼠皮下移植瘤的影响

用全反式视黄酸（ＡＴＲＡ）处理３株胃癌细胞株ＭＧｃ８０３、ＢＧＣ８２３和ＳＧＣ７９０１（ＡＴＲＡ终浓度为１０－６ｍｏｌ／Ｌ）连续２０ｄ，再移植于６～８周龄的ＢＡＬＢ／ｃ裸小鼠后肢皮下，观察移植瘤生长情况，４周后处死裸小鼠，解剖肿瘤并行组织学检查。结果表明，ＡＴＲＡ体外处理胃癌细胞能延长其移植瘤的潜伏期和倍增时间，有效地抑制移植瘤的生长，抑制率分别为５１．４％、６６．０％和８８．６％，抑制作用的强弱次序与移植瘤的恶性程度正好相反

Effects of all trans relinoic acid on the liver metastatic model of gastric cancer cells, BGC-823 and MKN-45

目的　研究全反式视黄酸 (ATRA)对胃癌细胞实验性肝转移能力的影响。方法　将胃癌细胞BGC 82 3和MKN 45注射于裸小鼠脾包膜下 ,隔日给裸小鼠灌服ATRA。术后 6周处死裸小鼠 ,解剖脾脏及肝脏肿瘤并用免疫组织化学方法测定其肿瘤血管密度 ;以放射免疫法测定血清癌胚抗原 (CEA)。结果　ATRA抑制胃癌细胞脾包膜下移植瘤的生长及其肝转移 ,抑制率分别为 5 0 .0 %(BGC 82 3)和 33.3% (MKN 45 ) ;使脾移植瘤和肝转移瘤的肿瘤血管密度降低。ATRA还可使肝转移模型鼠血清CEA含量分别下降 5 0 .2 4% (BCG 82 3)和 48.10 % (MKN 45 )。结论　ATRA能有效抑制胃癌细胞的实验性肝转移 ,从而抑制胃癌细胞分泌CEA以及抑制肿瘤血管的形成。 【英文摘要】 Objective To investigate the effects of all trans retinoic acid (ATRA) on experimental liver metastatic ability of gastric cancer cells. Methods BGC 823 and MKN 45 cells were injected into spleen subcapsule of nude mice, who were administrated ATRA every other day after operation. Six weeks after operation, the nude mice were executed. Tumors in spleen and liver were examined pathologically and microtumor vessel density (MVD) was measured by using immunohistochemical method and serum carcino embryonic ..

护士倒班前后智力活动的比较

受试者为20—35岁倒班和正常白班护士共46名。倒班组在上小夜班前和次日大夜班后分别给予一次智力活动测验,正常白班组两次测验的时间和时间间隔与倒班组相同。测验结果表明:倒班组两次测验仅在“数字符号”项目有部分显著性差异;而正常白班第二次测验在“数字符号”和“计数”两个项目,均较第一次测验取得显著进步。这提示倒班对于注意、记忆和反应操作速度可能有些影响

二陈汤方加减对2型糖尿病并发脂肪肝模型大鼠血糖、血脂、胰岛素抵抗以及肝功能和肝脏脂肪变的影响

目的:观察具有化痰利湿、活血化瘀功效的二陈汤方加减干预2型糖尿病并发脂肪肝大鼠血糖、血脂、胰岛素的抵抗状况,以及脂肪肝模型病理形态学的变化。方法:实验于2004-04/11在厦门大学医学院动物实验中心完成。①选用健康Wistar大鼠40只,7周龄,雌雄各半。随机抽取12只为正常对照组,喂以普通饲料(其中各成分质量分数如下:碳水化合物0.6、蛋白质0.22,脂肪0.1,其他0.08,脂肪以豆油为主),其余则喂养高热量饲料(其中各成分质量分数如下:碳水化合物0.4,蛋白质0.13,脂肪0.4,其他0.07,脂肪以动物油脂为主)。2个月后,正常对照组的大鼠一次性腹腔注射0.1mol/LpH4.2枸椽酸缓冲液,其余28只大鼠则一次性腹腔注射链脲佐菌素25mg/kg。72h后查血糖≥16.7mmol/L为糖尿病大鼠,造模成功24只。②将24只糖尿病大鼠随机分为2组:模型组和二陈汤方加减组,每组12只。正常对照组喂以普通饲料,其余2组均喂养高热量饲料。二陈汤方加减组大鼠按8mL/(kg·d)剂量灌胃二陈汤方加减药物,由陈皮、半夏、茯苓、僵蚕及地龙等组成(上述中药由本院中药房提供),由本院药剂科制备成浓缩液(含生药2g/mL),1次/d;正常对照组和模型组灌胃等量的自来水,均连续干预16周。③然后采用ELISA法测血胰岛素水平,计算肝指数(肝湿重/体质量×100%)和胰岛素敏感指数ln｛[1/(空腹血糖×空腹胰岛素)]｝;光镜下评估肝脂肪变性和炎症坏死程度。炎症活动度计分[慢性肝炎炎症活动度及纤维化计分方案炎症活动度计分分为汇管区(P)、小叶内(L)、碎屑坏死(PN)及桥接坏死(BN)4项,每项依病变轻、中、重程度分别计以1,3,4分,计分公式为P+L+2(PN+BN)]。按照上海九强生物技术有限公司提供相应试剂盒说明书测定血清三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、谷草转氨酶、谷丙转氨酶、白蛋白及总蛋白水平。分别于造模前、造模后8周及造模后16周采用氧化酶法测定空腹血糖。④计量资料、等级资料、率差异比较分别采用两样本均数t检验、秩和检验、两样本率差别的统计意义检验。结果:由于造模失败脱失4只,最终进入结果分析36只。①肝指数:造模后16周模型组和二陈汤方加减组明显高于正常对照组(P<0.01)。②空腹血糖水平:造模后8和16周模型组及二陈汤方加减组均明显高于造模前和正常对照组(P<0.01)。造模后8和16周二陈汤..

和谐社会建设背景下的红十字会功能定位——基于X市红十字会调查的思考

构建和谐社会为我国红十字会发展带来了前所未有的机遇与挑战。问题的关键在于红十字会能否根据和谐社会建设的需要进行角色调适,使功能定位更加准确合理。基于X市红十字会的调查,我们发现红十字会在服务功能上存在“大而全“、在传播功能上存在“散而浅“的问题,这淡化了红十字会的组织特色,并衍生出专业化水平不高以及社会认知度不够等问题。因此,在构建和谐社会背景下,红十字会需要进一步转变发展思路,从“大而全“走向“专而精“,充分发挥组织优势,打造核心竞争力,以获得可持续发展。厦门大学“211工程”三期建设项目“公共管理与公共政策”成果;得到X市红十字会资

尿细胞角蛋白19在膀胱移行细胞癌术后监测中的应用

目的　评价尿细胞角蛋白19片段(CK19,又称CYFRA2 1 1)在膀胱移行细胞癌(BTCC)术后监测中的作用。方法　对67例保留膀胱的BTCC术后患者定期行膀胱镜检查发现2 1例复发,采用电化学发光免疫分析(ECLIA)技术测定复发组(2 1例)、未复发组(4 6例)和正常对照组(2 4例)的尿CKl9含量。结果　复发组尿CKl9的平均值为(19.60±18.5 7) μg/L ,明显高于未复发组(2 .3 3±1.64 ) μg/L和正常对照组(1.82±0 .83 ) μg/L。复发组与未复发组及正常对照组比较,差异有统计学意义(P <0 .0 1)。尿CKl9对BTCC复发的敏感性为90 .5 % ,特异性为76.0 %。结论　尿CKl9是监测BTCC术后复发较为敏感、特异而且快速无创的指

深度闭合性肾裂伤的非手术治疗

总结25例深度闭合性肾裂伤的救治经验。认为对深度闭合性肾裂伤,可根据具体情况,在严密监护下行非手术治疗,效果满意

Fine root distribution, seasonal pattern and production in a native forest and monoculture plantations in subtropical China

作者简介: 杨玉盛(1964～ ) , 男, 福建仙游人, 博士, 教授, 主要从事亚热带常绿阔叶林C、N 循环研究。E2mail: [email protected] tt. fj. cn 致谢: 福建农林大学蔡丽平、林瑞余、谢锦升, 陈爱玲、陈清山、邹双全、李春林, 硕士研究生郭剑芬、刘艳丽、岳永杰、于占源、江淼华、卢豪良等, 及莘口教学林场郑燕明、刘春华、王巧珍、陈辉等参加了项目研究[中文文摘] 通过对福建三明格氏栲天然林及在其采伐迹地上营造的33年生格氏栲人工林和杉木人工林细根分布、季节动态与净生产力进行的为期3a(1999～2001)的研究,结果表明,格氏栲天然林、格氏栲和杉木人工林活细根生物量分别为4.944t/hm2、3.198t/hm2和1.485t/hm2,死细根生物量分别为3.563t/hm2、2.749t/hm2和1.287t/hm2;死细根生物量占总细根生物量的比例分别为41.9%、46.2%和46.4%;0.05);活细根生物量最大值均出现在3月份,最小值一般出现在5～7月份或11～翌年1月份间。0～10cm表土层格氏栲天然林活细根生物量高达295.65g/m2,分别是格氏栲人工林和杉木人工林的2.4倍和8.1倍;该层格氏栲天然林活细根生物量占全部活细根生物量的59.8%,均高于格氏栲人工林(39.07%)和杉木人工林(24.51%)。格氏栲天然林、格氏栲人工林和杉木人工林细根分解1a后的干重损失率分别为68.34%～80.13%、63.51%～77.95%和47.69%～60.78%;年均分解量分别为8.747、5.143和2.503t/hm2;死亡量分别为8.632、5.148和2.492t/hm2;年均净生产量分别为8.797、5.425和2.513t/hm2,年周转速率分别为1.78、[英文文摘]In the last decades, large scale native forests have been converted to fast growing and high yield commercial forest plantations to meet the demands for timber, fuel material, and other forest products. Some silvicultural measures, such as planting pure stands, clear cutting and slash burning, have been widely applied during this conversion. Yield decline and land deterioration in such disturbed ecosystem has become serious. In this context, the ecological comparisons between native forests and monoculture plantations are necessary in providing the implications for sustainable management. Few studies on fine roots dynamics have been conducted within these ecosystems though the importance of fine roots in carbon and nutrient cycling has been increasingly emphasized due to their rapid turnover rates. Two 33 year old plantations, Chinese fir (Cunninghamia lanceolata, CF) and Castanopsis kawakamii (CK), were chosen to examine fine root (005). An early spring flush of root biomass (March) was found in these three forests, and the minimum value mainly occurred during May July or November January. For the NF, 598% of root biomass was found in the top soil of 0～10 cm, a layer that maximum depth distribution difference among these forests occurred, where root biomass of the NF was 237 times and 812 times as much as that of the CK and the CF, respectively. Percentages of original mass lost during the first year of decomposition ranged from 4379%～5631% for the FH to 6834%～8013% for the NF Mean annual root decomposition, mortality and production ranged from 847 t/(hm 2·a), 8632 t/(hm 2·a) and 95 t/(hm 2·a) in the NF to 2503 t/(hm 2·a), 2492 t/(hm 2·a) and 2513 t/(hm 2·a) in the CF. The mean root turnover rate ranged from 178 times/a in the NF to 169 times/a in the CF.国家自然科学基金资助项目(30170770);福建省自然科学基金资助项目(B0110025);高等学校优秀青年教师教学科研奖励计划资助项目;福建省基础研究重大资助项目(2000-F-004

Nutrient dynamics of decomposing leaf litter in natural and monoculture plantation forests of Castanopsis kawakamii in subtropical China

通过对中亚热带格氏栲天然林 (natural forest of Castanopsis kawakamii,约 15 0 a)、格氏栲和杉木人工林 (monocultureplantations of C.kawakamii and Cunninghamia lanceolata,33年生 )凋落叶分解过程中养分动态的研究表明 ,各凋落叶分解过程中 N初始浓度均发生不同程度的增加后下降 ;除格氏栲天然林中其它树种叶和杉木叶 P浓度先增加后下降外 ,其它均随分解过程而下降 ;除杉木叶外 ,其它类型凋落叶的 Ca和 Mg浓度呈上升趋势 ;凋落叶 K浓度均随分解过程不断下降。养分残留率与分解时间之间存在着指数函数关系 xt=x0 e- kt。凋落叶分解过程中各养分释放常数分别为 :N (k N) 0 .6 78～ 4 .0 88;P (k P)0 .6 2 1～ 4 .30 8;K(k K) 1.4 0 8～ 4 .4 2 1;Ca (k Ca) 0 .799～ 3.75 6 ;Mg (k Mg) 0 .837～ 3.894。除杉木叶外 ,其它凋落叶分解过程中均呈 k K>k P>k N>k Mg>k Ca的顺序... 【英文摘要】 Nutrient dynamics of decomposing leaf litter was studied in two 33-year-old plantations, Chinese fir (Cunninghamia lanceolata, CF) and Castanopsis kawakamii (CK), and compared with that of an adjacent natural forest of Castanopsis kawakamii (NF, ~150 year old) in Sanming, Fujian, China. During the decomposition, varying degree of initial increase followed by decrease of N concentrations was observed in leaf litter, while initial increase and then decrease of P concentration was only found in leaves of other...教育部高等学校优秀青年教师奖资助项目;; 教育部高等学校骨干教师资助计划资助项目 ;; 福建省重大基础研究资助项目 (2 0 0 0 F 0 0 4)~

Experimental study on reperfusion of intraocular lens

作者简介: 祁明信, 男, 1945 年7 月 出生, 教授、主任医师、博士研究生 导师, 主要从事白内障的基础与临 床研究。联系电话: 0591-83570887; E-mail:qihuang@netease. com 通讯作者: 黄秀榕,E-mail:[email protected][中文文摘]目的开展晶状体再灌注的离体和动物实验研究,并对再灌注人工晶状体技术进行评价。方法采用新鲜离体幼兔眼、离体猪眼、新西兰白兔眼,应用自行研制的人工晶状体材料,进行以下实验:(1)体外固化实验;(2)晶状体前囊膜微型撕囊及其稳固性实验;(3)经微型前囊膜开口超声乳化吸出晶状体内容物实验;(4)活的新西兰白兔眼内人工晶状体再灌注实验。结果(1)按硅酮聚合物与固化剂50:1的比例可获得柔软、弹性好、固化时间短(完全固化时间为60min)的注入材料;(2)晶状体前囊膜1.8～2.0mm的连续环形撕囊口具有较好的稳定性,可经该微型开口吸出晶状体内容物并灌注材料;(3)超声能量18%、流量25mL·min-1、负压120mmHg(1kPa=7.5mmHg)为晶状体内容物经微型前囊膜开口吸出的最佳条件;(4)注入灌注材料后可形成由晶状体囊膜包裹的、置换原晶状体皮质和核的、新的再灌注人工晶状体。结论采用再灌注人工晶状体的方法可进行新型人工晶状体再灌注,可为治疗白内障和老视提供参考。[英文文摘]Objective To carry out the experimental study on reperfusion of intraocular lens(IOLs) in vitro or in animal,and to assess the technique of IOLs reperfusion.Methods The following experiments were performed by using self-developed materials in fresh rabbit eyes and pig eyes in vitro,as well as in eyes of alive New-Zea-land rabbits:(1)Solidification study of self-developed material in vitro;(2)Continuous circular capsulorhexis(CCC) in anterior capsule of lens and its stability;(3)Draw of lens contents via phaco through mini-CCC;(4)IOLs ref illing in the eyes of alive New-Zea land rabbits. Results(1) Thematerialwhich was soft, springy and short-term solidification(full solidification time was 60 minutes) were obtained in certain proportion of geland solidified agent(50:1) in vitro; ( 2)The CCC in anterior capsule of lens with 1.8-2.0 mm diameter had very good stability. The lens contents were drawn and the materialwere refilled through themini-CCC; (3) The best conditions of drawing out lens contents through m ini-CCC were phaco energy 18% , flow 25 mL·min- 1, and negative pressure 120 mmH g (1kPa=7.5 mmHg);(4) The new refilled IOLs, which were wrapped by capsule of lens and were replaced original cortex and nucleus of lens, were obtained after thematerial refillied. Conc lusion. New IOLs are refilled through this method, which can prov ide reference for the treatment of cataract and presbyopia.福建省科技三项费用;教育厅重点资助项目基金资助(编号:K98041