Up-regulation and clinical relevance of novel helicase homologue DHX32 in colorectal cancer

Xiamen Bureau for Science and Technology [A0000033]Background: This study aimed to find novel biomarkers for colorectal cancer. Methods: Fluorescent mRNA differential display PCR (DD-PCR) was used to screen the genes differentially expressed in colorectal cancer tissues and their adjacent tissues. The differentially expressed genes were confirmed by real-time PCR and then their clinical relevance (such as association with tumor location and lymph gland metastasis) was further investigated. Results: We identified by DD-PCR a novel RNA helicase, DHX32, which showed higher expression in colorectal cancer tissues than their adjacent tissues, and this result was confirmed by real time RT-PCR. In addition, we found that the level of DHX32 gene expression in colorectal cancer was significantly associated with cancer location, lymph gland metastasis, cancer nodal status, differentiation grade, and Dukes, stage. Conclusion: DHX32 may play an important role in the development of colorectal cancer and could serve as a novel biomarker for colorectal cancer after additional investigation

原发性肝癌组织cyclinB1表达水平及其临床诊断意义

目的探讨细胞周期蛋白B1(cyclinB1)在肝癌组织的表达及其临床诊断意义。方法用逆转录聚合酶链反应(RTPCR)检测40例原发性肝细胞癌患者癌组织及其癌旁组织cyclinB1mRNA的表达。结果90%(36/40)癌组织及85%(34/40)癌旁组织cyclinB1mRNA呈阳性表达,癌组织cyclinB1mRNA表达量为0.531±0.015,明显高于癌旁组织0.263±0.023,两者比较有统计学意义(P<0.05)。cyclinB1mRNA表达与肝癌的病理分级、淋巴转移与否有关,而与性别、年龄、肿瘤大小、癌栓形成与否无关。结论在肝癌发生、发展中,cyclinB1可能起着重要作用,cyclinB1表达水平可作为肝癌分级、分化及癌转移的辅助衡量指标之一

Clinical application of CD11b determination by flow cytometry before and after PTCA and CA

目的探讨冠心病(CHD)患者经皮冠状动脉腔内成形术(PTCA)、冠状动脉造影(CA)手术前及术后中性粒细胞(PMN)和单核细胞CD11b表达变化及临床意义。方法选择66例CHD患者,其中41例行PTCA手术、25例行CA手术,23例健康体检者为研究对象。采用流式细胞仪检测健康体检者及CHD患者PTCA和CA手术前3d、手术当天、术后30min、术后1d、术后3d外周血PMN和单核细胞表面CD11b的表达。结果CHD患者外周血PMN和单核细胞CD11b平均荧光强度(MFI)较正常对照组明显升高,差异具有显著性(P0.05)。结论PMN和单核细胞表面CD11b含量评价选择平均荧光强度比选择百分率更能反映CD11b的变化。CHD患者PTCA术后确有炎症反应发生,CD11b表达可作为PTCA后炎症反应发生有临床应用价值的指标。Objective: To investigate changes in the expression of CD11b on neutrophils and monocytes before and after PTCA and CA in patients with coronary heart disease (CHD).Methods:Forty-one patient sunderwent percutaneous coronary angioplasty (PTCA). Twenty-five patients underwent coronary angiographic (CA). Twenty-three people were healthy as control. Peripheral venous blood samples were obtained at intraday, 30min, 24h and 72h before and after PTCA and CA. Flow-cytometric methods were used to measure CD11b on neutrophils and monocytes. Rsults: The levels of CD11b on neutrophils and monocytes were significantly higher in UA patients with CHD compared to controls (P0.05). Conclusion: The mean intensity of fluorescence is better than percentage to evaluate the content of CD11b on neutrophils and monocyte. PTCA triggers leukocytes activation in circulation. CD11b can reflect the acute inflammatory activity aridses which arises after PTCA

尿细胞角蛋白19在膀胱移行细胞癌术后监测中的应用

目的　评价尿细胞角蛋白19片段(CK19,又称CYFRA2 1 1)在膀胱移行细胞癌(BTCC)术后监测中的作用。方法　对67例保留膀胱的BTCC术后患者定期行膀胱镜检查发现2 1例复发,采用电化学发光免疫分析(ECLIA)技术测定复发组(2 1例)、未复发组(4 6例)和正常对照组(2 4例)的尿CKl9含量。结果　复发组尿CKl9的平均值为(19.60±18.5 7) μg/L ,明显高于未复发组(2 .3 3±1.64 ) μg/L和正常对照组(1.82±0 .83 ) μg/L。复发组与未复发组及正常对照组比较,差异有统计学意义(P <0 .0 1)。尿CKl9对BTCC复发的敏感性为90 .5 % ,特异性为76.0 %。结论　尿CKl9是监测BTCC术后复发较为敏感、特异而且快速无创的指

SNP的研究现状及在MMPs研究中的应用

对单核苷酸多态性(singlenucleotidepolymorphisms,SNPs)的研究分析近几年被广泛应用于生物及医学研究的诸多领域,筛查SNPs的方法很多,各具特色,并一直不断地发展.本文对筛查SNP的几种常用及最新方法做一简要介绍,其中包括PCR-RFLP,分子信标等.细胞外基质的降解和改变是肿瘤转移的基本条件,基质金属蛋白酶(matrixmetalloproteinases,MMPs)是一类依赖锌离子的蛋白水解酶,可以降解细胞外基质、基底膜、以及间质基质,在肿瘤转移中具有重要作用.有些MMP基因序列存在单基因多态性即SNP现象,且最终影响MMP蛋白的功能.对MMPs的单基因多态性研究为进一步从分子水平研究MMPs的结构和功能及MMPs与肿瘤转移的关系提供了一个新的方向

从生态自然观看细菌的耐药

伴随抗菌药物强大的抗感染疗效的同时,细菌等病原体对抗菌药物的耐药性问题日渐显露出来。运用生态自然观辨证地对细菌进行剖析,旨在突破传统思维认识细菌耐药性产生本质,从而更加有效,更加科学地控制细菌耐药的发生

Comparison of gene frequencies of 15 STR loci between patients with primary gastric adencarcinomas and the unrelated locals from Xiamen

目的比较15个短串联重复序列(STR)基因座基因频率在原发性胃腺癌患者和厦门地区正常人群中的分布,推测与胃腺癌相关的基因。方法123份血样采自本地区无癌家族史的健康人群,39份血样采自本地区胃腺癌患者。用聚合酶链反应(PCR)复合扩增结合四色荧光检测方法对血样DNA进行基因型分析,调查本地区健康人群和胃腺癌患者人群的基因频率分布,并根据两者的该15个基因座等位基因频率分布的差异性,推测易感连锁和抗性连锁的等位基因。结果厦门地区胃腺癌患者的TH01、vWA和FAG基因座的等位基因的分布与该地区健康人群比较差异有统计学意义(P<0·01)。在个别等位基因比较中,胃腺癌人群TH01-7的基因频率为0·0385,健康人群TH01-7的基因频率为0·2642,两者差异有统计学意义(P<0·01),相对危险度(RR)=0·1115;胃腺癌人群vWA-15基因频率0·0513,健康人群vWA-15的基因频率0·2927,两者差异有统计学意义(P<0·01),RR=0·1307;胃腺癌人群FAG-18的基因频率为0·1026,健康人群FAG-18的基因频率为0·0163,两者差异有统计学意义(P<0·01),RR=6·8998。结论TH01-7与胃腺癌相关联,其附近可能存在胃腺癌抗性基因;vWA-15附近有可能存在与胃腺癌相关的抗性基因;FAG-18与胃腺癌相关联,其附近可能存在胃腺癌易感基因。Objective To compare the gene frequencies of 15 STR loci between patients with primary gastric adencarcinomas and the unrelated locals from Xiamen in order to search for the genes correlated to the gastric adencarcinomas.Methods The control group consisted of 123 unrelated locals and the testing group was composed of 39 gastric adencarcinomas suffers. All genotypes of the sample DNA were analyzed by gene scan technology and multiplex PCR method with 4-colored fluorescence-labeled primers. All the polymorphic alleles of these 15 STR loci in unrelated healthy locals and patients with primary gastric adencarcinomas had been investigated. The sensitive or resistant genetic factors were inferred according to the statistical difference with distribution of allele frequencies.Results It showed that there were statistic differences (P<0.01)between controls and testing groups in allele frequencies of the three loci: TH01, vWA and FAG. The further exploration of the separated locus revealed that the gene frequency of TH01-7 in the gastric adencarcinomas suffers was 0.0385,but 0.2642 in the control group[P<0.01 and relative risk(RR)=0.1115];the gene frequency of vWA-15 in the gastric adencarcinomas suffers was 0.0513,but 0.2927 in the control group(P<0.01 and RR=0.1307);the gene frequency of FAG-18 in the gastric adencarcinomas suffers was 0.1026,but 0.0163 in the control group(P<0.01 and RR= 6.8998). Conclusions It is very possible that TH01 alleles may be associated with gastric adencarcinomas and it is possible that there is a resistant gene to gastric adencarcinomas near the region of TH01-7 locus; there is a resistant gene of gastric adencarcinomas near the region of vWA-15 locus; FAG alleles may be associated with gastric adencarcinomas and perhaps there is a sensitive gene of gastric adencarcinomas near FAG-18 locus

闽南肝癌高发区肝细胞癌与HBV复制的相关性分析

目的分析闽南肝癌高发区乙型肝炎病毒 (HBV)复制与原发性肝细胞癌 (PHCC)的关系。方法用实时荧光定量聚合酶链反应 (PQ- PCR)技术测定 6 1例 PHCC患者、40 7例不同病程的 HBV感染者及 17例健康人血清中 HBV DNA的含量 ,对照分析 HBV标志物 (HBVM) ,同时检测 PHCC患者抗 - HCV- Ig G和 HCV RNA。结果PHCC组 HBV DNA阳性率高达 80 .3% (49/ 6 1) ,高于其他肝病组 ,差异具显著性 (P<0 .0 2 ) ,HBV DNA含量各组间差异无显著性。PHCC组抗 - HCV- Ig G和 HVC RNA阳性率为 0。结论闽南肝癌高发区 PHCC患者 HBV DNA阳性率较高 ,HBV感染并持续复制 ,可能是该地区 PHCC的主要致病因

戊型肝炎病毒核酸阳性血浆经输血传播感染恒河猴的研究

目的 了解戊型肝炎病毒(HEV)核酸阳性血浆对灵长类动物的感染性和致病性。 方法 对抗-HEV IgM阳性而IgG阴性志愿献血员血浆进行HEV RNA检测,并将存在病毒血症献血员的10ml血浆静脉输入健康恒河猴,观察其对恒河猴的感染性和致病性。 结果 从1份抗-HEV IgM阳性而IgG阴性志愿献血员血浆中分离出HEV基因IV型RNA片段。该份血浆输入恒河猴后,恒河猴出现典型急性肝炎生物化学和病理表现,病毒血症,血清抗-HEV IgM和IgG抗体阳转。 结论 HEV病毒血症献血员血浆输入可以引起灵长类动物的HEV感染以及急性肝炎,提示HEV经输血传播的可能性

集美大学2002级福建籍新生肝炎感染现状

目的　了解集美大学 2 0 0 2级福建籍新生甲、乙、戊 3型肝炎感染情况。方法　采用血清流行病学调查方法。结果　学生中抗HAV -IgG ,HBsAg和抗HEV -IgG阳性率分别为 77.0 8% ,1 5 .2 3 % ,1 7.1 5 % ,地区差异明显 ;HAV ,HEV无性别差异 ,而HBV以男性为高 ;农村HAV和HBV感染率均高于城镇 ,而HEV感染率无城乡差别。结论　应该加强对本地区大学生的甲、乙肝预防 ,急需研究出一种有效的戊肝疫