基于决策树-马尔科夫模型的麻疹疫苗接种经济学评价

目的评估麻疹疫苗接种的成本-效益和成本-效果。方法建立决策树-马尔科夫模型,以麻疹疫苗接种为研究策略,不接种为对照策略,以2014年浙江省出生人口数为队列人群,估计不同策略的长期成本和...国家自然科学基金面上项目(71874147; 81573257);; 福建省自然科学基金面上项目(2017J01133

敲除衣藻内源基因和表达外源基因的载体构建方法及应用

本发明提出了一种敲除衣藻内源基因和表达外源基因的载体构建方法及应用，该载体包含一个潮霉素抗性标记基因，标记基因两端都有一个来自RubisCO基因的splicing donor序列(TCCATTTGCAG/GATGTTCGA)，三个串联重复的终止密码子，以及一个转录终止子。该载体用Spel Ⅰ和Cla Ⅰ双酶切后产生的1.9kb片段电击转化莱茵衣藻细胞，即使载体插入衣藻基因内含子中插入载体也成为mRNA一部分，基因转录在三个串联重复终止密码子和转录终止子处停止。与没有SIS的2.6kb AphVⅢ插入载体相比，新构建的载体提高了突变体转录终止效率。</p

添加晶种对沼气发酵液磷回收的影响研究

以畜禽养殖废水经厌氧消化处理后的沼气发酵液为研究对象,采用曝气吹脱调节pH的处理方式。晶种采用海泡石粉、沸石粉和凹凸棒石粉3种天然矿物材料。考察了不同pH条件、晶种添加量和不同初始磷负荷对磷回收的影响,并利用扫描电镜-能谱分析仪（SEM-EDX）对产物进行了表征分析。结果表明：3种晶种材料中,凹凸棒石粉对磷回收的促进效果最好。在pH从7.5到8.5之间海泡石粉、沸石粉和凹凸棒石粉均可将Rp（t）提高10%以上,8.5到9.5之间,添加海泡石粉和沸石粉对Rp（t）的提升幅度降低,添加凹凸棒石粉可以达到14%左右的提升幅度。海泡石粉和沸石粉添加量增大后Rp（t）有小幅的提升,但变化不规律,凹凸棒石粉的最佳添加量为0.92 g/L。初始PO43--P为90 mg/L时凹凸棒石粉对磷回收有最好的促进效果。添加凹凸棒石粉的产物在SEM图片上可看到明显有晶体覆盖,EDX图上出现了明显的强度较大的P组分峰,从微观的角度证实了添加凹凸棒石粉的促进作用

复苏植物旋蒴苣苔J结构域蛋白编码基因BhDNAJC2的克隆、表达与功能

热激蛋白(HSP)是一类在受到逆境刺激后大量表达的蛋白质,能够帮助蛋白质正确折叠,促使变性蛋白质降解,缓解逆境胁迫对生物体的损伤。为揭示热激蛋白在耐旱的复苏植物中的保护作用,该研究对复苏植物旋蒴苣苔(Boea hygrometrica)HSP40家族中J结构域蛋白Bh DNAJC2的编码基因进行了克隆、表达与功能分析。Real-time PCR检测表明,该基因受脱水、低温、热激等多种逆境条件和脱落酸(ABA)诱导表达。Bh DNAJC2-YFP定位于细胞质、内质网和细胞核。过表达Bh DNAJC2的拟南芥(Arabidopsis thaliana)株系在干旱、热激、盐胁迫和碱胁迫下均表现出明显的抗逆性。综上所述,Bh DNAJC2可能在旋蒴苣苔抗旱、耐热及耐盐碱等胁迫反应中起关键作用

HV12p-rPA血栓靶向脂质体的制备及体内溶栓实验

将通过蛋白质工程获得的抗凝溶栓双功能蛋白水蛭素12肽-瑞替普酶(HV12p-rPA)制备成靶向脂质体,并考察其体内溶栓效果。用薄膜分散-超声法制备HV12p-rPA脂质体,通过正交设计优化处方,采用碳二亚胺法偶联抗纤维蛋白原单克隆抗体(McAbSZ-65)制备HV12p-rPA靶向脂质体,采用大鼠颈总动脉血栓模型,考察HV12p-rPA靶向脂质体的体内溶栓效果。结果:最佳处方中HV12p-rPA脂质体的粒径为(142.45&plusmn;1.20)nm,Zeta电位为(-30.63&plusmn;0.48)mV,平均包封率为(91.59&plusmn;1.39)%。靶向脂质体组(0.48&plusmn;0.083)mg在相同剂量下(80k IU/kg)与PBS空白对照组(2.04&plusmn;0.114)mg、游离HV12p-rPA组(1.2&plusmn;0.100)mg和普通HV12p-rPA脂质体组(0.74&plusmn;0.089)mg分别比较,其血栓干重明显减轻(P&lt;0.05);靶向脂质体组与5倍剂量(400k IU/kg)的游离HV12p-rPA组(0.52&plusmn;0.084)mg比较,其血栓干重较之偏轻(P&gt;0.05)。制备出的靶向HV12p-rPA脂质体具有体内靶向溶栓效果

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies