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复苏植物旋蒴苣苔J结构域蛋白编码基因BhDNAJC2的克隆、表达与功能
Authors
孙冬梅
张振南
+5 more
朱燕
王波
邓馨
陈世璇
龚月桦
Publication date
1 January 2015
Publisher
Abstract
热激蛋白(HSP)是一类在受到逆境刺激后大量表达的蛋白质,能够帮助蛋白质正确折叠,促使变性蛋白质降解,缓解逆境胁迫对生物体的损伤。为揭示热激蛋白在耐旱的复苏植物中的保护作用,该研究对复苏植物旋蒴苣苔(Boea hygrometrica)HSP40家族中J结构域蛋白Bh DNAJC2的编码基因进行了克隆、表达与功能分析。Real-time PCR检测表明,该基因受脱水、低温、热激等多种逆境条件和脱落酸(ABA)诱导表达。Bh DNAJC2-YFP定位于细胞质、内质网和细胞核。过表达Bh DNAJC2的拟南芥(Arabidopsis thaliana)株系在干旱、热激、盐胁迫和碱胁迫下均表现出明显的抗逆性。综上所述,Bh DNAJC2可能在旋蒴苣苔抗旱、耐热及耐盐碱等胁迫反应中起关键作用
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of Botany,Chinese Academy Of Sciences
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Last time updated on 27/10/2022