Analysis and Design of the Company Financial Management System

随着时代的进步，人工管理公司账务的方法已经不能满足人们的需求，人工管理保密性差，在填写账单时易出错，且没有统一的规范，使得在查询账务时效率极低，并且时间一长，就会产生大量的文件和数据，造成管理和维护上的困难。在计算机技术飞速发展的今天，使用计算机代替人工管理公司账务已经成为必然的趋势。使用计算机管理账务有人工管理无法比拟的优点：填写账单格式统一，易于管理；保密性好，没有权限无法查看公司账务；查询效率高，使用数据库存储数据大大提高了查询的效率，只须一个SQL语句就能实现人工管理很难达到的结果；存储量大。 本系统是用MacromediaDreamweaver和MyEclipse工具开发的，B/S...With the progress of the times, manual management of accounts can no longer meet the needs of people. Artificial management has poor confidentiality, low accuracy, and the format is not uniform. So it will be slow when you query the accounts. And over time, it will produce a large number of documents and data. Resulting in management and maintenance difficulties. With The rapid development of comp...学位：工程硕士院系专业：软件学院_工程硕士(软件工程)学号：X201023049

Other Spaces in Legal Pedagogy

There is an increasing focus upon the material and metaphoric spatial dimensions of various academic disciplines, including law. This essay considers the spatial dimensions of legal pedagogy, focusing on Critical Race Theory (CRT). The essay first explains the critical program in law and how CRT grows out of it. The essay then suggests that the critical program, and especially CRT, is as much a human geographic or spatial construct as it is a social, political or historic one, and briefly describes the nature of human geography and legal geography. It next considers how metaphors for understanding CRT\u27s position in legal pedagogy are found in some of Foucault\u27s work on geography. In Des Espaces Autres ( Other Spaces ), Foucault argues that there are three distinct social spaces in society: real spaces, utopias, and heterotopias. What unites them, Foucault suggests, is a space that includes elements of all of these spaces, a space that he calls the mirror. Applying this frame, this essay posits that CRT, both the explicit courses on the topic and the discipline itself, should be re-mapped , that is, barriers to its inclusion in the broader legal pedagogy should be eliminated. In this way, CRT can function as a Foucauldian mirror rather than a heterotopia in legal pedagogy

Determination of Pseudostellarin B in Pseudostellaria heterophylla by HPCE and Study on Its Fingerprints Analysis

建立了测定太子参中环肽Pseudostellarin B含量的高效毛细管电泳(HPCE)分析方法,对10种不同产地太子参中环肽Pseudostellarin B的含量进行了测定。HPCE工作条件:采用未涂层熔融石英毛细管(内径75μm,有效长度50cm),分离电压为15kV,柱温20℃,二极管阵列检测器(DAD)检测波长为203nm,缓冲液为20mmol/L硼砂(pH=9.3)溶液。在选定的工作条件下,环肽Pseudostellarin B浓度与其响应信号值之间具有较好的线性相关性(Y=0.6357X+2.546,R=0.9985),加标回收率在93.8%~105.6%之间。在此基础上进行了太子参HPCE指纹图谱研究,采用中药指纹图谱相似度计算软件对不同太子参样品的HPCE指纹图谱进行相似度计算,以系统生成的对照指纹图谱为对照模板,10份样品中有8份的相似度在0.90以上,说明该方法可用于太子参质量控制。A modern HPCE analysis method was developed for determinating cyclic peptide Pseudostellarin B in Pseudostellaria heterophylla(Miq.) Pax.Separation and determination were carried out by HPCE under the following conditions: bared fused silica capillary (50 cm×75 μm i.d.),20 mmol/L borate (pH=9.3)as buffer,the run voltage is +15 kV,detection length of UV at 203 nm,and column temperature of 20 ℃.The results indicated that the developed method was simple,accurate and reliable for the determination of Pseudostellarin B with a good linearity(Y=0.6357X+2.546,R=0.9985),and the recovery ranges of 93.8%～105.6%.Based on this method,the HPCE fingerprints of Pseudostellaria heterophylla (Miq.) Pax were established.The fingerprints were compared by the software of the similarity evaluation system for chromatographic fingerprint.The fingerprint congruence coefficients of 8 electropherograms in 10 were above 0.90.The method of HPCE fingerprint is reliable and accurate.国家自然科学基金项目(20235020);; 国家科技部攻关项目(2003BA759C

Study on the Preparation of Salvianolic Acid B of High Purity

目的:建立高纯度丹酚酸B的最佳制备工艺流程。方法:通过比较几种不同大孔吸附树脂对丹酚酸B的吸附及洗脱性能,筛选出最佳树脂并对该树脂分离纯化丹酚酸B的工艺参数进行优化。结果:在本文所确定的最佳工艺条件下,丹酚酸B回收率为90.6%,纯度高达91.8%,稳定性良好,可作为丹参制剂的原料药应用。结论:该工艺操作简便,适用于工业化生产,有利于促进丹酚酸B的开发利用。Objective:The best technology of the preparation of salvianolic acid B was established.Methods:A series of macroporous adsorption resins were compared on their adsorption and desorption properties for salvianolic acid B and the optimal type resin had been selected. The technical parameters for purification of salvianolic acid B with the selected type resin were studied. Results:Under this condition, the recovery is 90.6% and the purity of salvianolic acid B is 91.8%. The product has a good stability and could be used for preparation directly.Conclusion:This method is simple and could be used for industrial production, promoting the exploitation and utilization of salvianolic acid B.国家自然科学基金重点项目(No.20235020);; 青岛市重点实验室建设项目(No.04-2-JS-135

Preparative Separation of Salvianolic Acid B from Salvia miltiorrhiza by High Speed Counter-Current Chromatorgraphy

采用高速逆流色谱法分离纯化丹参水溶性成分丹酚酸类物质,制备丹酚酸B化学对照品。分离采用的溶剂系统为正己烷-乙酸乙酯-水-甲醇(1.5:5:5:1.5),上相做固定相,下相做流动相,流速为1.7 mL/min,仪器转速850 rpm,进样量80 mg,纯度用HPLC方法测定。结果表明:一次分离可制备63.4 mg丹酚酸B,其纯度为98.6%。该方法操作简单,可作为高纯度丹酚酸B化学对照品的制备分离方法。Salvianolic acid B,a major bioactive component in Danshen(Salvia miltiorrhiza Bge.),was isolated and purified by high-speed counter-current chromatography(HSCCC) in semi-preparative scale from the crude extract of Danshen.Separation was performed with a two-phase solvent system composed of hexane-ethyl acetate-water-methanol(1.5:5:5:1.5,v/v) by eluting the lower aqueous phase at a flow-rate of 1.7 mL/min and a revolution speed of 850 rpm with injection volume of 80 mg.After the proceeding of separation,63.4 mg salvianolic acid B was obtained,and analyzed by normalization method according to HPLC areas giving a purity of 98.6%.HSCCC is a recommendable method to prepare and purify the active constituents from Salvia miltiorrhiza.国家自然科学基金重点项目(20235020);; 厦门市科技创新重点项目(3502Z20031096

不同区段HIV-1 env基因在Bac-to-Bac昆虫细胞杆状病毒表达系统中的表达及检测

利用昆虫细胞杆状病毒表达系统 ,将从一株HIV - 1阳性克隆质粒中获得的几个HIV包膜蛋白基因片段 ,克隆入转移载体中得到重组病毒。用此重组病毒感染昆虫细胞后表达出 3种HIV包膜蛋白 ,即GP12 0 - 41P、GP41T、GP41P ,分别含有HIV - 1包膜糖蛋白GP12 0及部分GP41,删除了N端 12个疏水氨基酸的GP41和仅有主要表位约 2 40个氨基酸的GP41。收获后分别以Western -blotting和EIA检测 ,有较好的免疫学活性 ,其中GP41T的活性最强。该实验为HIV包膜蛋白的结构研究提供了依据 ,加以改进后可能有免疫检测的价值

我国科技期刊媒体融合现状与发展方向

摘要【目的】调研国际科技期刊媒体融合发展现状，探寻我国科技期刊媒体深度融合发展方向。【方法】基于我国科技期刊实际、政策导向与专家意见，对我国科技期刊的媒体融合现状进行相关文献调研、案例分析对比。【结果】明确媒体融合概念、特点、发展历程及国内外发展状况，分析现阶段我国科技期刊融合进程中的不足，提出我国科技期刊媒体融合发展方向与着眼点。【结论】媒体融合具有帕累托效应，需尽早建立科技期刊融合效果评价体系; 相较于国外科技期刊集团化运作、立体化网络运营与矩阵化传播模式，我国科技期刊媒体融合具有自身特色，但融合质量参差不齐、运营管理薄弱; 可依据&ldquo;1 个总趋势、5 个特质、N 个创新点&rdquo;的思路促进我国科技期刊媒体融合的转型升级。</p

Preparation and Characteristic Research of Immobilized Acetylcholinesterase

本研究以期研制出能重复使用的固定化乙酰胆碱酯酶(ACHE),为天然产物复杂体系中ACHE抑制剂筛选新方法的发展奠定基础.以氨基化硅胶(APS-SI)微球为载体,戊二醛为交联剂对乙酰胆碱酯酶进行交联固定化,并研究了酶的最佳固定化条件和固定化酶的性质.结果表明,0.05 g氨基化硅胶微球载体,用戊二醛溶液活化6 H后,在给酶量5 u,28℃固定16 H条件下,得到固定化酶的活性最大.固定化酶在常温(20~40℃),以及较宽PH范围内(PH 6~10)均具有较高的活性,并且具有良好的保存稳定性和可重复利用率,为基于固定化靶酶亲和-色谱质谱联用分析快速筛选乙酰胆碱酯酶抑制剂新方法的发展奠定了基础.A new method for preparation of a reusable immobilized acetylcholinesterase(AchE) was developed in order to lay a foundation for screening AchE inhibitors from natural products.AchE was immobilized on aminated silica gel microspheres with glutaraldehyde as cross-linking reagent.The optimum conditions and properties of immobilized enzyme were investigated.The optimal immobilization process was as follows: 0.05 g of aminated silica gel microspheres was activated by glutaraldehyde solution for 6 h,and mixed with AChE solution with the activity of 5 U.Then,the mixture was shook for 16 h at 28 ℃.The immobilized enzyme produced by this method had the highest enzyme activity,and it could remain better activity with a wide range of pH(pH 6～10) at room temperature(20～40 ℃).The immobilized AChE showed higher storage stability and could be used repeatedly.国家自然科学基金(No.20905017);国家海洋局青年基金(No.2010140);海洋一所基本科研业务专项(No.2010G25)资助项目---

Classification of Panax Quinquefolium.L Samples by Cluster Analysis

建立西洋参药材分类方法;分别采用电感耦合等离子体质谱(ICP-MS)法、高效液相色谱(HPLC)法以及蒽酮-硫酸分光光度法,对12个西洋参样品中的15种无机元素、7种人参皂苷和多糖进行测定,用SPSS聚类分析法对12个西洋参样品进行聚类分析;结果表明:以4种不同的聚类变量进行聚类分析,其聚类分组结果并不一致,将几种有效成分综合为聚类变量,对西洋参进行分类更为科学合理;聚类分析法是西洋参分类分析的有效方法,对西洋参品种与质量的鉴定有一定的理论意义和实用价值。A classification method was developed for the quality assessment of Panax Quinquefolium.L.The contents of fifteen elements,seven ginsenosides and polysaccharide in twelve Panax Quinquefolium.L samples were determined by ICP-MS,HPLC and anthrone-sulfuric acid spectrophotometry,respectively.The cluster analysis was applied for the classification of 12 Panax Quinquefolium.L samples.The analysis results showed that the classified results were different with different cluster variables and it was found more reasonable to integrate several active components as cluster variables for the classification.The cluster analysis was useful for the classification of Panax Quinquefolium.L