Localizing Japanese toads in a mountainous terrain using drone-based radiotelemetry

山に棲む小動物の位置を空から推定する --生物に装着した電波発信機をドローンで捜索--. 京都大学プレスリリース. 2023-06-09.Monitoring the movement of small animals is a fundamental aspect of ecological studies as well as spatially explicit conservation and management. However, this remains a challenging task especially in mountainous terrains. Although drone-based radiotelemetry (DRT) is employed to localize animals, its application in mountainous terrains is limited by the collision risks associated with undulating terrains as well as the obstruction of signals by dense vegetation and steep slopes. We addressed these challenges by generating fine-scale three-dimensional maps and moving vertically mounted directional antennas in a double grid pattern, scanning both in longitudinal and latitudinal grids. This new DRT system was helpful in localizing four adult Japanese toads (Bufo japonicus) living in hiding places typical of mountainous terrains. All toads were located within 1–60 days of being released. Transmitter signals were detected within two consecutive flights (three flights in one case). Instances of transmitter detection were significantly biased when the drone was facing either direction of the double-grid path, indicating that the double-grid pattern had reduced detection failure. The absolute localization error (n = 48) of 22.4 ± 21.0 m (44.8 ± 42% of the transmitter-to-receiver distance) was lower than that reported in a previous study conducted in a similar mountainous terrain

Detection and accurate localization of harmonic chipless tags

We investigate the detection and localization properties of harmonic tags working at microwave frequencies. A two-tone interrogation signal and a dedicated signal processing scheme at the receiver are proposed to eliminate phase ambiguities caused by the short signal wavelength and to provide accurate distance/position estimation even in the presence of clutter and multipath. The theoretical limits on tag detection and localization accuracy are investigated starting from a concise characterization of harmonic backscattered signals. Numerical results show that accuracies in the order of centimeters are feasible within an operational range of a few meters in the RFID UHF band

Design And Practical Implementation Of Harmonic-Transponder Sensors

Harmonic radar is a nonlinear detection technology that transmits and receives radio-frequency (RF) signals at orthogonal frequencies, so as to suppress the undesired clutters, echoes and electromagnetic interreferences due to multipath scattering. Its implementation generally comprises a nonlinear tag (i.e, a harmonic transponder), which picks the interrogation signal at specific fundamental frequency (f0) and converts it into a high/sub-harmonic signal (nf0). Such a technology has been successfully applied to tracking small insects and detection of electrically-small objects in the rich-scattering environment. Similarly, a harmonic sensor is used to interrogate electrically-small and passive sensors, of which the magnitude and peak frequency of output harmonics (e.g., second harmonic) are functions of the parameter to be sensed. A harmonic tag or sensor comprises one or multiple antennas, a frequency modulator, a sensor, a microchip and matching networks. Here, we propose and experimentally validate compact, low-cost, low-profile, and conformal hybrid-fed microstrip antennas for the harmonics-based radar and sensor systems. The proposed 98 microstrip antennas are based on a simple single-layered and hybrid-feed structure. By optimizing the feed position and the geometry of microstrip patch, the fundamental mode and particular higher-order modes can be excited at the fundamental frequency and the second harmonic. We have derived the analytical expressions for calculating the antennas’ resonant frequencies, which have been verified with numerical simulations and measurements. Our results show that the proposed hybrid-feed, single-layered microstrip antennas, although having a compact size and a low profile, can achieve descent realized gain (1.2 – 3.5 dB), good impedance matching (return loss \u3c -15 dB), high isolation (\u3c-20 dB), and favorable co/cross-polarization properties. The proposed microstrip antennas may benefit various size-restricted harmonic transponders used for harmonic radars, harmonic sensors, medical implants, passive radio-frequency identification (RFID), and internet-of-things (IoT) applications

Linking Husbandry and Behavior to Enhance Amphibian Reintroduction Success

Wildlife in captivity has a long history of benefiting global conservation goals. Captive animals can raise awareness and appreciation for the conservation of endangered species. Additionally, captive animals can be used as source populations to reintroduce animals back to the wild or to supplement existing wild populations. The rapid increase in amphibian species threatened with imminent extinction has necessitated the creation of dozens of captive-breeding programs. The focus of this dissertation has integrated topics across the spectrum of animals in captivity and the wild, and the results provide useful recommendations for conservation action. First, I describe how market pressures over a 28-year period are causing meteoric increases in the prices of amphibians sold in the pet trade, indicating a high risk of overexploitation. Pet amphibians may facilitate greater understanding and appreciation of amphibians, but the pet trade must be sustainable. Improving amphibian husbandry will increase the number of captive-bred animals available in the pet trade, and it will allow greater production of threatened species for reintroductions. Secondly, by performing a systematic review of husbandry for 289 amphibian species native to the US, I identified a critical lack in taxon-specific husbandry and developed husbandry research prioritizations. Next, I used a combination of laboratory and field studies to examine domestication processes in amphibians by comparing defensive behaviors in two species of captive-bred and wild poison frog. Captive-bred amphibians had significantly reduced defensive behaviors compared to wild conspecifics, likely resulting from habitation processes related to their husbandry. Finally, I performed three reintroductions of the critically endangered Wyoming Toad (Anaxyrus baxteri) in Wyoming, US. I demonstrated how providing a transitionary period, called a soft-release, to captive-bred toads moving to a novel, wild environment can improve reintroduction success. My work illustrates how improving our understanding of the nexus between captivity and the wild can improve conservation action for endangered species

Perspectives and challenges for the use of radar in biological conservation

Radar is at the forefront for the study of broad-scale aerial movements of birds, bats and insects and related issues in biological conservation. Radar techniques are especially useful for investigating species which fly at high altitudes, in darkness, or which are too small for applying electronic tags. Here, we present an overview of radar applications in biological conservation and highlight its future possibilities. Depending on the type of radar, information can be gathered on local- to continental-scale movements of airborne organisms and their behaviour. Such data can quantify flyway usage, biomass and nutrient transport (bioflow), population sizes, dynamics and distributions, times and dimensions of movements, areas and times of mass emergence and swarming, habitat use and activity ranges. Radar also captures behavioural responses to anthropogenic disturbances, artificial light and man-made structures. Weather surveillance and other long-range radar networks allow spatially broad overviews of important stopover areas, songbird mass roosts and emergences from bat caves. Mobile radars, including repurposed marine radars and commercially dedicated ‘bird radars’, offer the ability to track and monitor the local movements of individuals or groups of flying animals. Harmonic radar techniques have been used for tracking short-range movements of insects and other small animals of conservation interest. However, a major challenge in aeroecology is determining the taxonomic identity of the targets, which often requires ancillary data obtained from other methods. Radar data have become a global source of information on ecosystem structure, composition, services and function and will play an increasing role in the monitoring and conservation of flying animals and threatened habitats worldwide

A Passive Harmonic Tag for Humidity Sensing

This paper describes a passive harmonic tag for radio frequency identification (RFID) and wireless sensor applications. The tag uses a dual polarized UHF patch antenna as an input antenna. One of the outputs is connected to a frequency doubler, which consists of a Schottky diode with its output connected to a patch tuned at twice the input frequency. The other output of the input antenna feeds a DC power harvested converter that drives an oscillator which modulates its output signal by controlling the bias point of the Schottky diode. The antenna’s output is also used as a humidity sensor. To achieve this, the antenna is loaded with an interdigital capacitor with humidity-dependent capacitance. The antenna is consequently detuned when humidity varies, and therefore the second harmonic power is received. The tag is manufactured using standard fiberglass substrate. The basic theory of harmonic tag operation is described and compared with the standard backscattering approach. Experimental results with a proof of concept using commercial components are presented

Movin\u27 & Groovin\u27 Salamanders: Conservation Implications of Large Scales and Quirky Sex

Mole salamanders (Ambystoma) and woodfrogs (Lithobates sylvaticus) are abundant in New England and depend on ephemeral wetlands for breeding. Their aquatic habitats have been well studied and are protected by several local and regional regulations. State endangered species laws also protect mabled salamanders (A. opacum), Jefferson salamanders (A. jeffersonianum), and blue-spotted salamanders (A. laterale). However, these amphbibians spend most of their adult lives in terrestrial habitats that remain poorly protected and elusive to researchers. In chapter 1, I developed a novel technique using passive integrated transponders for tracking small animals. I used this technique to track marbled salamanders walking up to 200 m from their breeding pond during post-breeding migrations. In Chapter 2, I examined the importance of multiple habitat variables for predicting the distributions of woodfrogs and spotted salamanders at 455 ponds in western Massachusetts. Based on a variable-comparison technique I developed, the best predictor for either species of amphibian was the amount of forest in the surrounding vii landscape. Both species were found more frequently in upland forests where the ponds are least protected by state and federal wetland regulations. In chapter 3, I used my data from chapter 2 and three other similar data sets to conduct an analysis of spatial scale and to parameterize a recently published resistant kernel model. The complex model parameterized by an expert panel did significantly worse than the null model. The distributions of both amphibians were best predicted by measuring the landscape at very large scales (over 1000 m). The most effective scales for conservation may be largest for organisms of intermediate dispersal capability. In chapter 4, I explored the evolution and genetics of the Jefferson/blue-spotted/unisexual salamander complex. I framed research into the fascinating unisexual reproductive system with a model that relates nuclear genome replacement, positive selection on hybrids, and biogeography of the species complex. I parameterized this model using genetic data taken from salamanders spanning Massachusetts and an individual-based breeding simulation. If paternal genomes are transmitted to offspring with the frequencies reported from laboratory experiments, then my model suggests that there must be strong selection favoring unisexuals with hybrid nuclei

Creating Successful Vernal Pools: A Literature Review and Advice for Practitioners

Vernal pools provide critical breeding habitat for amphibians adapted to temporary waters, but they seldom receive the same level of protection as permanent wetlands. In response to continued degradation and loss of pools, managers often attempt to mitigate losses through pool creation or restoration. However, mitigation efforts often fail to provide suitable aquatic habitat for vernal pool amphibians. We review the literature on pool creation in northeastern and central North America, highlighting how and why constructed pools often fail to support amphibian-related objectives. We recommend that practitioners consider the complex ecology of pool ecosystems and the historical and current distribution of pools and other wetlands in their local context before designing pool mitigation projects. Using vernal pool creation as a mitigation option should be a last resort (i.e., when elimination of natural pools is unavoidable). Monitoring should be target-specific and conducted for at least 5 years. Topographic, geologic, and other local factors affecting pool hydrology and ecology vary regionally; pool creation remains an imperfect science that will only advance by documenting failures and successes. We recommend an adaptive management approach to vernal pool creation in which the effectiveness of techniques is evaluated and refined based on research