SFADI: the Speckle-Free Angular Differential Imaging method

We present a new processing technique aimed at significantly improving the angular differential imaging method (ADI) in the context of high-contrast imaging of faint objects nearby bright stars in observations obtained with extreme adaptive optics (EXAO) systems. This technique, named "SFADI" for "Speckle-Free ADI", allows to improve the achievable contrast by means of speckles identification and suppression. This is possible in very high cadence data, which freeze the atmospheric evolution. Here we present simulations in which synthetic planets are injected into a real millisecond frame rate sequence, acquired at the LBT telescope at visible wavelength, and show that this technique can deliver low and uniform background, allowing unambiguous detection of $10^{-5}$ contrast planets, from $100$ to $300$ mas separations, under poor and highly variable seeing conditions ($0.8$ to $1.5$ arcsec FWHM) and in only $20$ min of acquisition. A comparison with a standard ADI approach shows that the contrast limit is improved by a factor of $5$. We extensively discuss the SFADI dependence on the various parameters like speckle identification threshold, frame integration time, and number of frames, as well as its ability to provide high-contrast imaging for extended sources, and also to work with fast acquisitions.Comment: Accepted for publication in Ap

Image Processing Instrumentation for Giardia lamblia Detection

Currently, the identification and enumeration of Giardia Iamblia cysts are based upon microscopic methods requiring individuals proficient in this area. It is a tedious process which consumes time that could be constructively used elsewhere. This project attempts to alleviate that burden by employing a computer to automatically process Indirect Fluorescent Antibody (IFA) prepared slides using digital image processing techniques. A computer controlled frame grabber, in conjunction with a CCD TV camera mounted on the epi-fluorescence microscope phototube, captures the light intensities of the objects in view under the microscope objective. The captured image is stored as pixels, with each pixel having a numerical value that can be altered using linear contrast enhancement and bit-slicing to emphasize the cysts and eliminate the majority of unwanted objects from the image. The altered image is then analyzed by a vector trace routine for typical area and perimeters characteristic to Giardia lamblia cysts. Objects in the image matching these characteristics are most likely cysts and are added to a running tally of the number of cysts present on the slide

Wavefront correction enables vibrational imaging of bacteria with multimode fibre probes

Raman spectroscopy is a valuable tool for non-invasive and label-free identification of sample chemical composition. Recently a few miniaturized optical probes emerged driven by the need to address areas of difficult access, such as in endoscopy. However, imaging modality is still out of reach for most of them. Separately, recent advances in wavefront shaping enabled different microscopies to be applied in various complex media including multimode fibers. Here we present the first and thinnest to date Raman fiber imaging probe based on wavefront shaping through a single multimode fiber without use of any additional optics. We image agglomerates of bacteria and pharmaceuticals to demonstrate the capability of our method. This work paves the way towards compact and flexible Raman endoscopy. © (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.Publisher PD

Machine vision applications in agriculture

Keynote paper. [Abstract]: With the trend of computers towards convergence with multimedia entertainment, tools for vision processing are becoming commonplace. This has led to the pursuit of a host of unusual applications in the National Centre for Engineering in Agriculture, in addition to work on vision guidance. These range from the identification of animal species, through the location of macadamia nuts as they are harvested and visual tracking for behaviour analysis of small marsupials to the measurement of the volume of dingo teeth

Microtesla MRI of the human brain combined with MEG

One of the challenges in functional brain imaging is integration of complementary imaging modalities, such as magnetoencephalography (MEG) and functional magnetic resonance imaging (fMRI). MEG, which uses highly sensitive superconducting quantum interference devices (SQUIDs) to directly measure magnetic fields of neuronal currents, cannot be combined with conventional high-field MRI in a single instrument. Indirect matching of MEG and MRI data leads to significant co-registration errors. A recently proposed imaging method - SQUID-based microtesla MRI - can be naturally combined with MEG in the same system to directly provide structural maps for MEG-localized sources. It enables easy and accurate integration of MEG and MRI/fMRI, because microtesla MR images can be precisely matched to structural images provided by high-field MRI and other techniques. Here we report the first images of the human brain by microtesla MRI, together with auditory MEG (functional) data, recorded using the same seven-channel SQUID system during the same imaging session. The images were acquired at 46 microtesla measurement field with pre-polarization at 30 mT. We also estimated transverse relaxation times for different tissues at microtesla fields. Our results demonstrate feasibility and potential of human brain imaging by microtesla MRI. They also show that two new types of imaging equipment - low-cost systems for anatomical MRI of the human brain at microtesla fields, and more advanced instruments for combined functional (MEG) and structural (microtesla MRI) brain imaging - are practical.Comment: 8 pages, 5 figures - accepted by JM