Characterization and prognostic value of mutations in exons 5 and 6 of the p53 gene in patients with colorectal cancers in central Iran

Background/Aims: We aimed to investigate the relation-ships among various mutations of the p53 gene and their protein products, histological characteristics, and disease prognosis of primary colorectal cancer in Isfahan, central Iran. Methods: Sixty-one patients with colorectal adenocarcinoma were enrolled in the study. Mutations of the p53 gene were detected by single-stranded conformation polymorphism and DNA sequencing. The protein stability was evaluated by immunohistochemistry. Patients were followed up to 48 months. Results: Twenty-one point mutations in exons 5 and 6 were detected in the tumor specimens of 14 patients (23%). Of those, 81% and 9.5% were missense and nonsense mutations, respectively. There were also two novel mutations in the intronic region between exons 5 and 6. In 11 mutated specimens, protein stability and protein accumulation were identified. There was a relationship between the type of mutation and protein accumulation in exons 5 and 6 of the p53 gene. The presence of the mutation was associated with an advanced stage of cancer (trend, p<0.009). Patients with mutated p53 genes had significantly lower survival rates than those with wild type p53 genes (p<0.01). Conclusions: Mutations in exons 5 and 6 of the p53 gene are common genetic alterations in colorectal adenocarcinoma in central Iran and are associated with a poor prognosis of the disease

Validation of the Anxiety Scale for Pregnancy in a Sample of Iranian Women

We investigated propagation of electromagnetic waves through composite structures with negative refractive index, the popular ”left-handed metamaterials”, for the case when there is a gradient of refractive index. We obtained the exact analytical solutions to the Helmholtz equation valid for arbitrary steepness of the graded interface between the positive and the negative index part. We analyzed the special case of matched impedances of the two constituent materials within the metamaterial composite. We derived analytical expressions for the field intensity, transmission and reflection coefficients and compared them with the results obtained by the numerical simulations using the Finite Element Method. The model allows for arbitrary spectral dispersion and lossy media.QC 20120126</p

بررسی تأثیر سین‌بیوتیک‌های موجود در ایران بر درمان اسهال حاد در کودکان 6 ماهه تا 5 ساله مراجعه کننده سرپایی به کلینیک

A survey on efficacy of available synbiotics in Iran in the treatment of acute diarrhea in 6 months to 5 years old outpatients Background: Acute diarrhea is a great public health problem. Diarrhea is a leading cause of death in children under five years of age globally with an estimated 1.5 million child deaths per year. Acute diarrhea defined as more than three loose or watery stools in 24 hours lasting not longer than 14 days.primary treatment of diarrhea is replacing of fluid and electrolyte with oral rehydration. Probiotics are traditionally defined as viable microorganisms that have a beneficial effect in the prevention and treatment of specific pathologic conditions when they are ingested. The aim of this study is surveying on available synbiotic (prebiotic plus probiotic) in Iran in the treatment of acute diarrhea. Because of extensive cost of synbiotic surveying on usefulness or uselessness of these products can be effectiveness on decrease of treatment’s cost for family and health system. Materials and Methods: This is a randomized double blind clinical trial.100 patient with acute diarrhea divided to two group of 6 months to 24 months and 24 months to 60 months.Each group devided to two subgroup and randomly assigned to receive specific synbiotic for their age.25 people who has 6 months to 24 months old,receive pedilact(10 drops per day for 5 day) and 25 people who has more than 24 months old recive kidilact (1 suchet per day for 5 day).Then duration of diarrhea lasting is noted and compared.Data was analysed with SPSS. Result: Difference in the mean of duration between two group was not significant (P=0.736 in 6 months to 24 months and P=0.920 in more than 24 months). The mean of duration was 4.02 in interventional group and 3.92 in control which shows the synbiotics were not effictiveness in acute diarrhea. Conclusion: Use of available synbiotic in Iran has no significant advantage vs absolute oral rehydarion therapy and just can increase the cost of treatment. Key Words: Probiotic, Prebiotic, Acute diarrhe

بررسی تاثیر برنامه خود مدیریتی مبتنی بر مدل 5A بر کیفیت زندگی سالمندان مبتلا به سندرم حاد کرونری شهر بجنورد

Introduction: Improve the quality of life in heart patients is the main objective of health improvement programs. Self-management education programs, the most important ways to improve and promote health. The aim of this study was to evaluate the effect of self-management 5A on the quality of life of elderly patients with acute coronary syndrome. Material&Methods: This clinical trial, two groups that 54 elderly patients with acute coronary syndrome patients admitted patients in the cardiology ward and CCU Imam Reza Hospital in Bojnourd for available selected and The method of permutation blocks into two groups (27 patients) and control (27), respectively. The intervention group self-management programs were based 5A. The control group received routine hospital. The instrument used in this study are lipad quality of life questionnaire. in the first and two months after the study was completed in two groups. Data using SPSS 20 software and Chi-square and Wilcoxon tests were analyzed with repeated measures analysis. Results: The findings showed that most of patients were female, married and uneducated. The mean age of patients 66/18 ± 4/68 years. The mean scores in all aspects of quality of life was higher in the intervention group after completion of the study. But statistically significant difference was not observed. Conclusion: This study demonstrated the effectiveness of self-management program 5A model on quality of life of elderly patients with acute coronary syndrome in the study period was not. Key words: 5 A self-management, quality of life, acute coronary syndrome, elderl

Evaluating the effectiveness of auraptene on beta amyloid-induced spatial memory impairment in rats

Introduction: Alzheimer's disease is an advanced and multifactorial neurodegenerative disease, which makes the largest group of learning and memory disorders. This disease is associated with the deposition of amyloid beta (Aβ) in the extracellular space and the formation of neurofibrillary tangles (NFTs) within the cellular body of neurons. Auraptene is a simple coumarin that performs many protective acts as a neurotrophic and acetyl cholinesterase inhibitor compound in the brain. This study was conducted to evaluate the protective effects of auraptene on the memory improvement in Aβ42-induced model of Alzheimer. Materials and Methods: In this study, 48 male Wistar rats (200-250 g) were used. Animals were divided into 6 groups, 8 animals each; Sham (non-injection cannulation), Negative control (Alzheimer induced by injection of 30 ng Aβ42 to the hippocampus), auraptene solvent as well as three therapeutic groups receiving 5, 10 and 25 mg / kg auraptene for 12 days after Aβ injection. The evaluation of spatial memory performance after Alzheimer's induction was performed by morris water maze behavioral test. Bcl-2 Bax ratio was measured by ELISA method to determine the expression of proteins involved in apoptosis. Results: Intraperitoneal administration of auraptene (5, 10 and 25 mg / kg) in therapeutic groups significantly decreased the time to reach the platform and increased the duration of the presence and distance traveled in the target quadrant compared to Alzheimer induced group. Also, ELISA results showed that expression of Bax protein in treatment groups was significantly lower than in Negative control group. While a significant increase was observed in the expression of Bcl-2 protein. Also, the ratio of Bax / Bcl-2 showed a significant decrease in the hippocampus of Auraptene treated groups. Conclusion: This study showed the memory enhancement effect of auraptene after induction of Alzheimer's disease. It seems that this effect could be through the increased expression of Bcl-2 protein and decrease in the expression of Bax protein

Inhibition of microglial activity alters spinal wide dynamic range neuron discharge and reduces microglial Toll-like receptor 4 expression in neuropathic rats

It is believed that neuropathic pain results from aberrant neuronal discharges although some evidence suggests that the activation of glia cells contributes to pain after an injury to the nervous system. This study aimed to evaluate the role of microglial activation on the hyper-responsiveness of wide dynamic range neurons (WDR) and Toll-like receptor 4 (TLR4) expressions in a chronic constriction injury (CCI) model of neuropathic pain in rats. Adult male Wistar rats (230 � 30 g) underwent surgery for induction of CCI neuropathy. Six days after surgery, administration of minocycline (10, 20, and 40 mg/kg, i.p.) was initiated and continued until day 14. After administration of the last dose of minocycline or saline, a behavioral test was conducted, then animals were sacrificed and lumbar segments of the spinal cord were collected for Western blot analysis of TLR4 expression. The electrophysiological properties of WDR neurons were investigated by single unit recordings in separate groups. The findings showed that after CCI, in parallel with thermal hyperalgesia, the expression of TLR4 in the spinal cord and the evoked response of the WDR neurons to electrical, mechanical, and thermal stimulation significantly increased. Post-injury administration of minocycline effectively decreased thermal hyperalgesia, TLR4 expression, and hyperresponsiveness of WDR neurons in CCI rats. The results of this study indicate that post-injury, repeated administration of minocycline attenuated neuropathic pain by suppressing microglia activation and reducing WDR neuron hyperresponsiveness. This study confirms that post-injury modulation of microglial activity is a new strategy for treating neuropathic pain

مقایسه اثر درمانی تری کلرواستیک اسید %40 با کرایوتراپی دردرمان زگیل کف پا در شهرستان سبزوار سال 1397

Introduction:This study compares the therapeutic effect of trichloroacetic acid 40%(TCA) with cryotherapy in the treatment of plantar warts. Method:Sixty patients were randomly divided into two groups after confirmation of plantar warts by dermatologist and inclusion criteria. A group of patients received trichloroacetic acid 40% at a dose of 1.5-1.5 per wart at each treatment for 4 weeks and the other group received liquid nitrogen with a dose of 1-2 cc per wart Every week, they received 8 weeks. The rate of change in symptoms and complications was evaluated after the last treatment and in each treatment cycle. Results:The results of this study showed that TCA 40% treatment had a similar effect to cryotherapy, although it was not statistically significant, but the complications, treatment cost, and duration of treatment with trichloroacetic acid 40% were less than cryotherapy have been. Conclusion:In general, it can be said that with regard to cost, the complications and the duration of treatment with trichloroacetic acid 40% can be a good alternative to cryotherapy

The Relationships Between Personality Traits With Women Sexual Satisfaction

Objectives Based on the reported studies, approximately 80 of marital disputes result from sexual dissatisfaction. It is helpful to identify factors predicting sexual satisfaction, especially in females, to prevent such problems. The current study aimed at examining simple and multiple factorial relationships between big 5 personality traits and sexual satisfaction in a population of married females in Mashhad, Iran. Methods Statistical population in the current study included all married females who lived in Mashhad. A total of 115 females were selected by the available sampling method. To collect data, the sexual satisfaction scale for women (SSS-W) and big 5 personality questionnaire (NEO) were employed. The data were analyzed by multiple regressions and validated by confirmatory factor analysis using AMOS-21 software and Pearson correlation coefficient using SPSS version 19.Results Findings of the current study indicated a significant relationship between the big 5 personality traits, including neuroticism (R=-0.407), extraversion (R=0.310), openness (R=0.232), agreeableness (R=0.258) and conscientiousness (R=0.389), and sexual satisfaction in females (P<0.0001). Stepwise multiple regression analyses that among the 5 independent variables, only 2 variables of neuroticism and conscientiousness could be used in the regression equation (R2=0.203, P<0.0001). Conclusion Findings of the current study indicated that females� sexual satisfaction can be predicted through their personality traits. Also, personality variables, especially neuroticism and conscientiousness, were recommended to examine the sexual incompatibility