Lipidomic data analysis: Tutorial, practical guidelines and applications

Lipids are a broad group of biomolecules involved in diverse critical biological roles such as cellular membrane structure, energy storage or cell signaling and homeostasis. Lipidomics is the -omics science that pursues the comprehensive characterization of lipids present in a biological sample. Different analytical strategies such as nuclear magnetic resonance or mass spectrometry with or without previous chromatographic separation are currently used to analyze the lipid composition of a sample. However, current analytical techniques provide a vast amount of data which complicates the interpretation of results without the use of advanced data analysis tools. The choice of the appropriate chemometric method is essential to extract valuable information from the crude data as well as to interpret the lipidomic results in the biological context studied. The present work summarizes the diverse methods of analysis than can be used to study lipidomic data, from statistical inference tests to more sophisticated multivariate analysis methods. In addition to the theoretical description of the methods, application of various methods to a particular lipidomic data set as well as literature examples are presented.This work has been supported by the European Research Council under the European Union's Seventh Framework Programme (FP/2007-2013) / ERC Grant Agreement n. 32073. Also, recognition from the Catalan government (grant 2014SGR1106) is acknowledged. JJ acknowledges a CSIC JAE-Doc contract cofounded by the FSE.Peer reviewe