ATRIBUT PELAYANAN JALAN TOL DALAM PENINGKATAN KUALITAS BERKENDARA DI JALAN TOL MAKASSAR

Abstract The construction of toll roads as an alternative road has been done to overcome the travel barriers. Similarly, the toll roads in the city of Makassar, which is the center of activities in Eastern Indonesia, support the movement of services and economic activities in the region. The evaluation of the toll road performance is needed to improve the quality of service, which can be done by performing an evaluation related to the user satisfaction. This study was conducted to obtain the desired service perceptions of toll road services using 175 respondents representing toll road users. Servqual and performance interest analysis matrix methods were performed for determining the attribute priority levels. The study showed that there is nine attributes which should be reflected as efforts to improve the quality in order to provide comfort for toll road users, namely: (1) traffic flow, (2) safety, (3) smoothness of road surface, (4) security from crime, (5) amount and toll gate facilities, (6) traffic signs, (7) road lighting, (8) accidents handling, and (9) road preservation. It is suggested that these attributes should be on priority by operator in delivering toll road service in order to enhance riding comfort. Keywords: toll road, toll road performance, service quality, toll road service  Abstrak Pembangunan jalan tol sebagai jalan alternatif sudah banyak dilakukan untuk mengatasi adanya hambatan perjalanan. Demikian halnya dengan keberadaan jalan tol di Kota Makassar, sebagai pusat kegiatan di Indonesia Timur, untuk mendukung layanan pergerakan serta kegiatan ekonomi. Evaluasi terhadap kinerja jalan tol perlu terus dilakukan untuk meningkatkan kualitas pelayanan, yang dapat dilakukan dengan melakukan evaluasi yang berorientasi pada tingkat kepuasan pengguna. Studi ini dilakukan untuk mendapatkan persepsi terhadap pelayanan yang dikehendaki dari layanan jalan tol dengan responden sekitar 175 orang yang dianggap mewakili pengguna jalan tol. Dalam menentukan tingkat prioritas atribut dilakukan analisis dengan menggunakan metode Servqual dan matriks analisis kepentingan performa. Hasil studi menunjukkan adanya 9 atribut yang mutlak harus tercermin sebagai usaha peningkatan kualitas agar memberikan kenyamanan pengguna jalan tol, yaitu: (1) kelancaran lalulintas, (2) keselamatan berkendara, (3) kerataan permukaan jalan, (4) keamanan dari tindak kriminal, (5) jumlah dan fasilitas gardu tol, (6) rambu lalulintas, (7) penerangan jalan, (8) penanganan kecelakaan, dan (9) ketanggapan perbaikan jalan yang rusak. Untuk meningkatkan kualitas dan kenyamanan pengguna jalan tol kesembilan atribut tersebut perlu dijadikan prioritas oleh operator dalam penyediaan pelayanan jalan tol. Kata-kata kunci: jalan tol, kinerja jalan tol, kualitas pelayanan,pelayanan jalan to

ATRIBUT PELAYANAN JALAN TOL DALAM PENINGKATAN KUALITAS BERKENDARA DI JALAN TOL MAKASSAR

Abstract The construction of toll roads as an alternative road has been done to overcome the travel barriers. Similarly, the toll roads in the city of Makassar, which is the center of activities in Eastern Indonesia, support the movement of services and economic activities in the region. The evaluation of the toll road performance is needed to improve the quality of service, which can be done by performing an evaluation related to the user satisfaction. This study was conducted to obtain the desired service perceptions of toll road services using 175 respondents representing toll road users. Servqual and performance interest analysis matrix methods were performed for determining the attribute priority levels. The study showed that there is nine attributes which should be reflected as efforts to improve the quality in order to provide comfort for toll road users, namely: (1) traffic flow, (2) safety, (3) smoothness of road surface, (4) security from crime, (5) amount and toll gate facilities, (6) traffic signs, (7) road lighting, (8) accidents handling, and (9) road preservation. It is suggested that these attributes should be on priority by operator in delivering toll road service in order to enhance riding comfort. Keywords: toll road, toll road performance, service quality, toll road service  Abstrak Pembangunan jalan tol sebagai jalan alternatif sudah banyak dilakukan untuk mengatasi adanya hambatan perjalanan. Demikian halnya dengan keberadaan jalan tol di Kota Makassar, sebagai pusat kegiatan di Indonesia Timur, untuk mendukung layanan pergerakan serta kegiatan ekonomi. Evaluasi terhadap kinerja jalan tol perlu terus dilakukan untuk meningkatkan kualitas pelayanan, yang dapat dilakukan dengan melakukan evaluasi yang berorientasi pada tingkat kepuasan pengguna. Studi ini dilakukan untuk mendapatkan persepsi terhadap pelayanan yang dikehendaki dari layanan jalan tol dengan responden sekitar 175 orang yang dianggap mewakili pengguna jalan tol. Dalam menentukan tingkat prioritas atribut dilakukan analisis dengan menggunakan metode Servqual dan matriks analisis kepentingan performa. Hasil studi menunjukkan adanya 9 atribut yang mutlak harus tercermin sebagai usaha peningkatan kualitas agar memberikan kenyamanan pengguna jalan tol, yaitu: (1) kelancaran lalulintas, (2) keselamatan berkendara, (3) kerataan permukaan jalan, (4) keamanan dari tindak kriminal, (5) jumlah dan fasilitas gardu tol, (6) rambu lalulintas, (7) penerangan jalan, (8) penanganan kecelakaan, dan (9) ketanggapan perbaikan jalan yang rusak. Untuk meningkatkan kualitas dan kenyamanan pengguna jalan tol kesembilan atribut tersebut perlu dijadikan prioritas oleh operator dalam penyediaan pelayanan jalan tol. Kata-kata kunci: jalan tol, kinerja jalan tol, kualitas pelayanan,pelayanan jalan to

Human Papillomavirus 16 E5 Induces Bi-Nucleated Cell Formation By Cell-Cell Fusion

Human Papillomaviruses (HPV) 16 is a DNA virus encoding three oncogenes – E5, E6, and E7. The E6 and E7 proteins have well-established roles as inhibitors of tumor suppression, but the contribution of E5 to malignant transformation is controversial. Using spontaneously immortalized human keratinocytes (HaCaT cells), we demonstrate that expression of HPV16 E5 is necessary and sufficient for the formation of bi-nucleated cells, a common characteristic of precancerous cervical lesions. Expression of E5 from non-carcinogenic HPV6b does not produce bi-nucleate cells. Video microscopy and biochemical analyses reveal that bi-nucleates arise through cell-cell fusion. Although most E5-induced bi-nucleates fail to propagate, co-expression of HPV16 E6/E7 enhances the proliferation of these cells. Expression of HPV16 E6/E7 also increases bi-nucleated cell colony formation. These findings identify a new role for HPV16 E5 and support a model in which complementary roles of the HPV16 oncogenes lead to the induction of carcinogenesis

Knowledge-to-Action Guides: Preparing Families to Be Partners in Making Educational Decisions

Posted in application of the KU Faculty's Open Access policy

The role of liquid based cytology and ancillary techniques in the peritoneal washing analysis: our institutional experience

Background The cytological analysis of peritoneal effusions serves as a diagnostic and prognostic aid for either primary or metastatic diseases. Among the different cytological preparations, liquid based cytology (LBC) represents a feasible and reliable method ensuring also the application of ancillary techniques (i.e immunocytochemistry-ICC and molecular testing). Methods We recorded 10348 LBC peritoneal effusions between January 2000 and December 2014. They were classified as non-diagnostic (ND), negative for malignancy-NM, atypical-suspicious for malignancy-SM and positive for malignancy-PM. Results The cytological diagnosis included 218 ND, 9.035 NM, 213 SM and 882 PM. A total of 8048 (7228 NM, 115SM, 705 PM) cases with histological follow-up were included. Our NM included 21 malignant and 7207 benign histological diagnoses. Our 820 SMs+PMs were diagnosed as 107 unknown malignancies (30SM and 77PM), 691 metastatic lesions (81SM and 610PM), 9 lymphomas (2SM and 7PM), 9 mesotheliomas (1SM and 8SM), 4 sarcomas (1SM and 3PM). Primary gynecological cancers contributed with 64% of the cases. We documented 97.4% sensitivity, 99.9% specificity, 98% diagnostic accuracy, 99.7% negative predictive value (NPV) and 99.7% positive predictive value (PPV). Furthermore, the morphological diagnoses were supported by either 173 conclusive ICC results or 50 molecular analyses. Specifically the molecular testing was performed for the EGFR and KRAS mutational analysis based on the previous or contemporary diagnoses of Non Small Cell Lung Cancer (NSCLC) and colon carcinomas. We identified 10 EGFR in NSCCL and 7 KRAS mutations on LBC stored material. Conclusions Peritoneal cytology is an adjunctive tool in the surgical management of tumors mostly gynecological cancers. LBC maximizes the application of ancillary techniques such as ICC and molecular analysis with feasible diagnostic and predictive yields also in controversial cases.info:eu-repo/semantics/publishedVersio

Human MLL/KMT2A gene exhibits a second breakpoint cluster region for recurrent MLL–USP2 fusions

Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq: PQ-2017#305529/2017-0Deutsche Forschungsgemeinschaft, DFG: MA 1876/12-1Alexander von Humboldt-Stiftung: 88881.136091/2017-01RVO-VFN64165, 26/203.214/20172018.070.1Associazione Italiana per la Ricerca sul Cancro, AIRC: IG2015, 17593Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPESCancer Australia: PdCCRS1128727CancerfondenBarncancerfondenVetenskapsrÃ¥det, VRCrafoordska StiftelsenKnut och Alice Wallenbergs StiftelseLund University Medical Faculty FoundationXiamen University, XMU2014S0617-74-30019C7838/A15733Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, SNSF: 31003A_140913CNIBInstitut National Du Cancer, INCaR01 NCI CA167824National Institutes of Health, NIH: S10OD0185222016/2017, 02R/2016AU 525/1-1Deutschen Konsortium für Translationale Krebsforschung, DKTK70112951Smithsonian Institution, SIIsrael Science Foundation, ISFAustrian Science Fund, FWF: W1212SFB-F06107, SFB-F06105Acknowledgements BAL received a fellowship provided by CAPES and the Alexander von Humboldt Foundation (#88881.136091/2017-01). ME is supported by CNPq (PQ-2017#305529/2017-0) and FAPERJ-JCNE (#26/203.214/2017) research scholarships, and ZZ by grant RVO-VFN64165. GC is supported by the AIRC Investigator grant IG2015 grant no. 17593 and RS by Cancer Australia grant PdCCRS1128727. This work was supported by grants to RM from the “Georg und Franziska Speyer’sche Hochsschulstiftung”, the “Wilhelm Sander foundation” (grant 2018.070.1) and DFG grant MA 1876/12-1.Acknowledgements This work was supported by The Swedish Childhood Cancer Foundation, The Swedish Cancer Society, The Swedish Research Council, The Knut and Alice Wallenberg Foundation, BioCARE, The Crafoord Foundation, The Per-Eric and Ulla Schyberg Foundation, The Nilsson-Ehle Donations, The Wiberg Foundation, and Governmental Funding of Clinical Research within the National Health Service. Work performed at the Center for Translational Genomics, Lund University has been funded by Medical Faculty Lund University, Region Skåne and Science for Life Laboratory, Sweden.Acknowledgements This work was supported by the Fujian Provincial Natural Science Foundation 2016S016 China and Putian city Natural Science Foundation 2014S06(2), Fujian Province, China. Alexey Ste-panov and Alexander Gabibov were supported by Russian Scientific Foundation project No. 17-74-30019. Jinqi Huang was supported by a doctoral fellowship from Xiamen University, China.Acknowledgments This work was supported by the Swiss National Science Foundation (grant 31003A_140913; OH) and the Cancer Research UK Experimental Cancer Medicine Centre Network, Cardiff ECMCI, grant C7838/A15733. We thank N. Carpino for the Sts-1/2 double-KO mice.Acknowledgements This work was supported by the French National Cancer Institute (INCA) and the Fondation Française pour la Recherche contre le Myélome et les Gammapathies (FFMRG), the Intergroupe Francophone du Myélome (IFM), NCI R01 NCI CA167824 and a generous donation from Matthew Bell. This work was supported in part through the computational resources and staff expertise provided by Scientific Computing at the Icahn School of Medicine at Mount Sinai. Research reported in this paper was supported by the Office of Research Infrastructure of the National Institutes of Health under award number S10OD018522. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The authors thank the Association des Malades du Myélome Multiple (AF3M) for their continued support and participation. Where authors are identified as personnel of the International Agency for Research on Cancer / World Health Organization, the authors alone are responsible for the views expressed in this article and they do not necessarily represent the decisions, policy or views of the International Agency for Research on Cancer / World Health Organization.We are indebted to all members of our groups for useful discussions and for their critical reading of the manuscript. Special thanks go to Silke Furlan, Friederike Opitz and Bianca Killing. F.A. is supported by the Deutsche For-schungsgemeinschaft (DFG, AU 525/1-1). J.H. has been supported by the German Children’s Cancer Foundation (Translational Oncology Program 70112951), the German Carreras Foundation (DJCLS 02R/2016), Kinderkrebsstiftung (2016/2017) and ERA PerMed GEPARD. Support by Israel Science Foundation, ERA-NET and Science Ministry (SI). A. B. is supported by the German Consortium of Translational Cancer Research, DKTK. We are grateful to the Jülich Supercomputing Centre at the Forschungszemtrum Jülich for granting computing time on the supercomputer JURECA (NIC project ID HKF7) and to the “Zentrum für Informations-und Medientechnologie” (ZIM) at the Heinrich Heine University Düsseldorf for providing computational support to H. G. The study was performed in the framework of COST action CA16223 “LEGEND”.Funding The work was supported by the Austrian Science Fund FWF grant SFB-F06105 to RM and SFB-F06107 to VS and FWF grant W1212 to VS

The contribution of HPV18 to cervical cancer is underestimated using high-grade CIN as a measure of screening efficiency

In one geographical area, 14 high-risk human papillomavirus types in cervical intraepithelial neoplasia (CIN2/3; n=139) and cervical squamous cell carcinoma (SCC; n=84) were analysed. HPV18 was more prevalent in SCC than CIN2/3 (OR 9.8; 95% confidence interval: 2.5–39). Other high-risk types prevalences corresponded in CIN2/3 and SCC. Evaluations using CIN2/3 as a measure of efficiency underestimate the contribution of HPV18 to SCC

The KMT2A recombinome of acute leukemias in 2023

Chromosomal rearrangements of the human KMT2A/MLL gene are associated with de novo as well as therapy-induced infant, pediatric, and adult acute leukemias. Here, we present the data obtained from 3401 acute leukemia patients that have been analyzed between 2003 and 2022. Genomic breakpoints within the KMT2A gene and the involved translocation partner genes (TPGs) and KMT2A-partial tandem duplications (PTDs) were determined. Including the published data from the literature, a total of 107 in-frame KMT2A gene fusions have been identified so far. Further 16 rearrangements were out-of-frame fusions, 18 patients had no partner gene fused to 5'-KMT2A, two patients had a 5'-KMT2A deletion, and one ETV6::RUNX1 patient had an KMT2A insertion at the breakpoint. The seven most frequent TPGs and PTDs account for more than 90% of all recombinations of the KMT2A, 37 occur recurrently and 63 were identified so far only once. This study provides a comprehensive analysis of the KMT2A recombinome in acute leukemia patients. Besides the scientific gain of information, genomic breakpoint sequences of these patients were used to monitor minimal residual disease (MRD). Thus, this work may be directly translated from the bench to the bedside of patients and meet the clinical needs to improve patient survival

An Integrated TCGA Pan-Cancer Clinical Data Resource to Drive High-Quality Survival Outcome Analytics

For a decade, The Cancer Genome Atlas (TCGA) program collected clinicopathologic annotation data along with multi-platform molecular profiles of more than 11,000 human tumors across 33 different cancer types. TCGA clinical data contain key features representing the democratized nature of the data collection process. To ensure proper use of this large clinical dataset associated with genomic features, we developed a standardized dataset named the TCGA Pan-Cancer Clinical Data Resource (TCGA-CDR), which includes four major clinical outcome endpoints. In addition to detailing major challenges and statistical limitations encountered during the effort of integrating the acquired clinical data, we present a summary that includes endpoint usage recommendations for each cancer type. These TCGA-CDR findings appear to be consistent with cancer genomics studies independent of the TCGA effort and provide opportunities for investigating cancer biology using clinical correlates at an unprecedented scale. Analysis of clinicopathologic annotations for over 11,000 cancer patients in the TCGA program leads to the generation of TCGA Clinical Data Resource, which provides recommendations of clinical outcome endpoint usage for 33 cancer types

Human Papillomavirus (HPV) 16 E6 Variants in Tonsillar Cancer in Comparison to Those in Cervical Cancer in Stockholm, Sweden

Background: Human papillomavirus (HPV), especially HPV16, is associated with the development of both cervical and tonsillar cancer and intratype variants in the amino acid sequence of the HPV16 E6 oncoprotein have been demonstrated to be associated with viral persistence and cancer lesions. For this reason the presence of HPV16 E6 variants in tonsillar squamous cell carcinoma (TSCC) in cervical cancer (CC), as well as in cervical samples (CS), were explored. Methods: HPV16 E6 was sequenced in 108 TSCC and 52 CC samples from patients diagnosed 2000–2008 in the County of Stockholm, and in 51 CS from young women attending a youth health center in Stockholm. Results: The rare E6 variant R10G was relatively frequent (19%) in TSCC, absent in CC and infrequent (4%) in CS, while the well-known L83V variant was common in TSCC (40%), CC (31%), and CS (29%). The difference for R10G was significant between TSCC and CC (p = 0.0003), as well as between TSCC and CS (p = 0.009). The HPV16 European phylogenetic lineage and its derivatives dominated in all samples (.90%). Conclusion: The relatively high frequency of the R10G variant in TSCC, as compared to what has been found in CC both in the present study as well as in several other studies in different countries, may indicate a difference between TSCC and CC with regard to tumor induction and development. Alternatively, there could be differences with regard to the oral an