67 research outputs found
RNAseq-Based Working Model for Transcriptional Regulation of Crosstalk between Simultaneous Abiotic UV-B and Biotic Stresses in Plants
Plants adjust their secondary metabolism by altering the expression of corresponding genes to cope with both abiotic and biotic stresses. In the case of UV-B radiation, plants produce protective flavonoids; however, this reaction is impeded during pattern-triggered immunity (PTI) induced by pathogens. Pathogen attack can be mimicked by the application of microbial associated molecular patterns (e.g., flg22) to study crosstalk between PTI and UV-B-induced signaling pathways. Switching from Arabidopsis cell cultures to in planta studies, we analyzed whole transcriptome changes to gain a deeper insight into crosstalk regulation. We performed a comparative transcriptomic analysis by RNAseq with four distinct mRNA libraries and identified 10778, 13620, and 11294 genes, which were differentially expressed after flg22, UV-B, and stress co-treatment, respectively. Focusing on genes being either co-regulated with the UV-B inducible marker gene chalcone synthase CHS or the flg22 inducible marker gene FRK1 identified a large set of transcription factors from diverse families, such as MYB, WRKY, or NAC. These data provide a global view of transcriptomic reprogramming during this crosstalk and constitute a valuable dataset for further deciphering the underlying regulatory mechanism(s), which appear to be much more complex than previously anticipated. The possible involvement of MBW complexes in this context is discussed
Design, synthesis, radiosynthesis and biological evaluation of Fenretinide analogues as anticancer and metabolic syndromepreventive agents
We thank the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement N° 675417 (PET3D project) for financial support of the project and the studentship of I.P. We also thank the British Heart Foundation for the project grant PG/16/90/32518.Peer reviewedPostprin
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NIRS for vicine and convicine content of faba bean seed allowed GWAS to prepare for marker-assisted adjustment of seed quality of German winter faba beans
Die antinutritiven Inhaltsstoffe Vicin und Convicin (V, C) in Samen von Winterackerbohnen wurden einer GWAS unterzogen. V-, C- und V + C-Werte von 189 Inzuchtlinien (fünf Umwelten) wurden mittels NIRS ermittelt. In diesen Linien kommt das starke „vc-“-Allel (VC1-Locus) nicht vor. Labor-Resultate von 646 Proben führten zu unserer NIRS-Kalibration, die gut für V und V + C tauglich war allerdings nicht tauglich für C. Die Erblichkeit war hoch für V und V + C (0,911; 0,868) und niedriger für C (0,737). Von den 2542 kartierten SNPs waren 47 signifikant mit V und einer mit V + C assoziiert. Vier SNPs, die nahe beim VC1-Lokus kartierten, waren für V signifikant. Anscheinend trugen nicht-„vc-“-Allele an diesem Locus zur V-Variation bei. Markergestützte Züchtung in diesem Genpool kann die V + C-Gehalt auf etwa 0,44 % reduzieren, im Vergleich zur aktuell niedrigsten Linie mit 0,55 %. Weitere Forschung wird zeigen, wie diese Ergebnisse der Agronomie und Züchtung dienlich sein werden.GWAS was applied to the antinutritive compounds vicine and convicine (V, C) in winter faba bean. V, C and V + C data for 189 inbred lines (five environments) were predicted by NIRS. These lines do not carry the strong “vc-“ allele (locus VC1). Lab data for 646 samples enabled our NIRS calibration, which performed well for V and V + C yet poor for C. Heritability was high (0.911; 0.868) for V and V + C and lower for C (0.737). From the 2542 mapped SNPs, 47 were significantly associated with V and one with V + C. Four SNPs mapped near to the VC1 locus and were significant for V. Seemingly, non-“vc-“ alleles at that locus contributed to V variation. Marker-assisted breeding with this germplasm can reduce the V + C content to about 0.44%, compared to the current lowest line with 0.55%. Further research will show inasmuch this can serve agronomy and breeding
MUSE spectroscopy and deep observations of a unique compact JWST target, lensing cluster CLIO
We present the results of a VLT MUSE/FORS2 and Spitzer survey of a unique compact lensing cluster CLIO at z = 0.42, discovered through the GAMA survey using spectroscopic redshifts. Compact and massive clusters such as this are understudied, but provide a unique prospective on dark matter distributions and for finding background lensed high-z galaxies. The CLIO cluster was identified for follow-up observations due to its almost unique combination of high-mass and dark matter halo concentration, as well as having observed lensing arcs from ground-based images. Using dual band optical and infra-red imaging from FORS2 and Spitzer, in combination with MUSE optical spectroscopy we identify 89 cluster members and find background sources out to z = 6.49. We describe the physical state of this cluster, finding a strong correlation between environment and galaxy spectral type. Under the assumption of an NFW profile, we measure the total mass of CLIO to be M200 = (4.49 ± 0.25) × 1014 M⊙. We build and present an initial strong-lensing model for this cluster, and measure a relatively low intracluster light (ICL) fraction of 7.21 ± 1.53 per cent through galaxy profile fitting. Due to its strong potential for lensing background galaxies and its low ICL, the CLIO cluster will be a target for our 110 h James Webb Space Telescope ‘Webb Medium-Deep Field’ (WMDF) GTO program.Publisher PDFPeer reviewe
A Strong-Lensing Model for the WMDF JWST/GTO Very Rich Cluster Abell 1489
We present a first strong-lensing model for the galaxy cluster RM
J121218.5+273255.1 (; hereafter RMJ1212; also known as Abell 1489).
This cluster is amongst the top 0.1\% richest clusters in the redMaPPer
catalog; it is significantly detected in X-ray and through the
Sunyaev-Zel'dovich effect in ROSAT and \emph{Planck} data, respectively; and
its optical luminosity distribution implies a very large lens, following
mass-to-light scaling relations. Based on these properties it was chosen for
the Webb Medium Deep Fields (WMDF) JWST/GTO program. In preparation for this
program, RMJ1212 was recently imaged with GMOS on Gemini North and in seven
optical and near-infrared bands with the \emph{Hubble Space Telescope}. We use
these data to map the inner mass distribution of the cluster, uncovering
various sets of multiple images. We also search for high-redshift candidates in
the data, as well as for transient sources. We find over a dozen high-redshift
() candidates based on both photometric redshift and the dropout
technique. No prominent () transients were found in the data
between the two HST visits. Our lensing analysis reveals a relatively large
lens with an effective Einstein radius of
(), in broad agreement with the scaling-relation expectations. RMJ1212
demonstrates that powerful lensing clusters can be selected in a robust and
automated way following the light-traces-mass assumption.Comment: 17 pages, 6 figures, 2 tables; To be submitte
The GALEX Ultraviolet Atlas of Nearby Galaxies
We present images, integrated photometry, surface-brightness and color
profiles for a total of 1034 nearby galaxies recently observed by the GALEX
satellite in its far-ultraviolet (FUV; 1516A) and near-ultraviolet (NUV; 2267A)
bands. (...) This data set has been complemented with archival optical,
near-infrared, and far-infrared fluxes and colors. We find that the integrated
(FUV-K) color provides robust discrimination between elliptical and
spiral/irregular galaxies and also among spiral galaxies of different
sub-types. Elliptical galaxies with brighter K-band luminosities (i.e. more
massive) are redder in (NUV-K) color but bluer in (FUV-NUV) than less massive
ellipticals. In the case of the spiral/irregular galaxies our analysis shows
the presence of a relatively tight correlation between the (FUV-NUV) color and
the total infrared-to-UV ratio. The correlation found between (FUV-NUV) color
and K-band luminosity (with lower luminosity objects being bluer than more
luminous ones) can be explained as due to an increase in the dust content with
galaxy luminosity.
The images in this Atlas along with the profiles and integrated properties
are publicly available through a dedicated web page at
http://nedwww.ipac.caltech.edu/level5/GALEX_Atlas/Comment: 181 pages, 10 figures, accepted for publication in ApJS (abstract
abridged
PEARLS: Low Stellar Density Galaxies in the El Gordo Cluster Observed with JWST
A full understanding of how unusually large "Ultra Diffuse Galaxies" (UDGs)
fit into our conventional understanding of dwarf galaxies remains elusive,
despite the large number of objects identified locally. A natural extension of
UDG research is the study of similar galaxies at higher redshift to establish
how their properties may evolve over time. However, this has been a challenging
task given how severely systematic effects and cosmological surface brightness
dimming inhibit our ability to study low-surface brightness galaxies at
high-. Here, we present an identification of low stellar surface density
galaxies (LDGs), likely the progenitors of local UDGs, at moderate redshift
with deep near-IR observations of the El Gordo cluster at with JWST.
By stacking 8 NIRCAM filters, we are able to achieve an apparent surface
brightness sensitivity of mag arcsec, faint enough to be
complete to the bright end of the LDG population. Our analysis identifies
significant differences between this population and local UDGs, such as their
color and size distributions, which suggest that UDG progenitors are bluer and
more extended at high- than at . This suggests that multiple
mechanisms are responsible for UDG formation and that prolonged transformation
of cluster dwarfs is not a primary UDG formation mechanism at high-.
Furthermore, we find a slight overabundance of LDGs in El Gordo, and, in
contrast to findings in local clusters, our analysis does not show a deficit of
LDGs in the center of El Gordo, implying that tidal destruction of LDGs is
significant between and .Comment: Resubmitted to ApJ after minor revision
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