Risk profiles and one-year outcomes of patients with newly diagnosed atrial fibrillation in India: Insights from the GARFIELD-AF Registry.

BACKGROUND: The Global Anticoagulant Registry in the FIELD-Atrial Fibrillation (GARFIELD-AF) is an ongoing prospective noninterventional registry, which is providing important information on the baseline characteristics, treatment patterns, and 1-year outcomes in patients with newly diagnosed non-valvular atrial fibrillation (NVAF). This report describes data from Indian patients recruited in this registry. METHODS AND RESULTS: A total of 52,014 patients with newly diagnosed AF were enrolled globally; of these, 1388 patients were recruited from 26 sites within India (2012-2016). In India, the mean age was 65.8 years at diagnosis of NVAF. Hypertension was the most prevalent risk factor for AF, present in 68.5% of patients from India and in 76.3% of patients globally (P < 0.001). Diabetes and coronary artery disease (CAD) were prevalent in 36.2% and 28.1% of patients as compared with global prevalence of 22.2% and 21.6%, respectively (P < 0.001 for both). Antiplatelet therapy was the most common antithrombotic treatment in India. With increasing stroke risk, however, patients were more likely to receive oral anticoagulant therapy [mainly vitamin K antagonist (VKA)], but average international normalized ratio (INR) was lower among Indian patients [median INR value 1.6 (interquartile range {IQR}: 1.3-2.3) versus 2.3 (IQR 1.8-2.8) (P < 0.001)]. Compared with other countries, patients from India had markedly higher rates of all-cause mortality [7.68 per 100 person-years (95% confidence interval 6.32-9.35) vs 4.34 (4.16-4.53), P < 0.0001], while rates of stroke/systemic embolism and major bleeding were lower after 1 year of follow-up. CONCLUSION: Compared to previously published registries from India, the GARFIELD-AF registry describes clinical profiles and outcomes in Indian patients with AF of a different etiology. The registry data show that compared to the rest of the world, Indian AF patients are younger in age and have more diabetes and CAD. Patients with a higher stroke risk are more likely to receive anticoagulation therapy with VKA but are underdosed compared with the global average in the GARFIELD-AF. CLINICAL TRIAL REGISTRATION-URL: http://www.clinicaltrials.gov. Unique identifier: NCT01090362

Human ribosomal RNA Primary structure analysis of the 28S rRNA gene and preliminary studies on the distribution of pseudouridine residues in the 18S and 28S rRNA molecules

SIGLEAvailable from British Library Document Supply Centre- DSC:DX174084 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Psc3 cohesin of Schizosaccharomyces pombe: cell cycle analysis and identification of three distinct isoforms

Cohesins are a group of proteins that function to mediate correct chromosome segregation, DNA repair and meiotic recombination. This report presents the amino acid sequence for the Schizosaccharomyces pombe cohesin Psc3 based on the translation of the cDNA sequence, showing that the protein is smaller than previously predicted. Interestingly, comparison of the amino acid and DNA coding sequences of Psc3 with fission yeast Rec11 meiotic region-specific recombination activator shows that both intron positioning within the genes and the amino-terminal half of the two proteins are highly conserved. We demonstrate that although the intergenic region upstream of the psc3+ start codon contains a consensus sequence for the cell-cycle regulatory MluI cell-cycle box, psc3+ transcription is not differentially regulated during the mitotic cell cycle. Finally, we demonstrate that an epitope-tagged version of Psc3 undergoes no major changes during the mitotic cell cycle. However, instead we identify at least three distinct isoforms of Psc3, suggesting that post-translational modification of Psc3 contributes to the regulation of cohesion function

Development of deliquoring method enhanced by electric and acoustic fields

A method developed uses acoustic and electric force fields to enhance filtration by increasing filtration capacity and by reducing production costs and consumption of energy.In both laboratory and pilot scale filtration the application of an electric field significantly increases the cake capacity of a vacuum disc filter.When constant DC electric field was applied across the ceramic capillary filter (CERAMEC), about 3.5-fold cake capacity values (with electric field: 250 kg D.S./m2h) were recorded during the filtration of titanium dioxide suspension using laboratory scale filter.Using continuous operation pilot scale filter values with and without electric field as its best were 60 kg D.S./m2h amd 10 kg D.S./m2h respectively.When an ultrasonic field was used during cake drying it was possible to obtain a rapid dewatering effect, up to 6% decrease in the cake moisture content, from 24 wt-% to 18 wt-%, using laboratore scale filter.In continuous operating pilot-scale filter the decrease in cake moisture content was about 3% from 24 to 21 wt-%.This research has revealed that the use of ultrasound is an effective way to increase the dewatering efficiency of the cake filtration process.The most important advantages of this process are obtained in the filtration of suspensions with very fine particles, whehe conventional systems are inefficient

Validation of a universal set of primers to study animal-associated microeukaryotic communities

7 pages, 2 figures, supporting information https://doi.org/10.1111/1462-2920.14733The application of metabarcoding to study animal‐associated microeukaryotes has been restricted because the universal barcode used to study microeukaryotic ecology and distribution in the environment, the Small Subunit of the Ribosomal RNA gene (18S rRNA), is also present in the host. As a result, when host‐associated microbial eukaryotes are analysed by metabarcoding, the reads tend to be dominated by host sequences. We have done an in silico validation against the SILVA 18S rRNA database of a non‐metazoan primer set (primers that are biased against the metazoan 18S rRNA) that recovers only 2.6% of all the metazoan sequences, while recovering most of the other eukaryotes (80.4%). Among metazoans, the non‐metazoan primers are predicted to amplify 74% of Porifera sequences, 4% of Ctenophora, and 15% of Cnidaria, while amplifying almost no sequences within Bilateria. In vivo, these non‐metazoan primers reduce significantly the animal signal from coral and human samples, and when compared against universal primers provide at worst a 2‐fold decrease in the number of metazoan reads and at best a 2800‐fold decrease. This easy, inexpensive, and near‐universal method for the study of animal‐associated microeukaryotes diversity will contribute to a better understanding of the microbiomeThis work was funded by a grant from the Canadian Institutes for Health Research (MOP‐42517). JdC was supported by a grant from the Tula Foundation to the Centre for Microbial Biodiversity and Evolution and the Marie Curie International Outgoing Fellowship FP7‐PEOPLE‐2012‐IOF‐331450 CAARLPeer Reviewe