1,160 research outputs found
The Distance Plus Joint Development Zone Formula: A Proposal for the Speedy and Practical Resolution of the East China and Yellow Seas Continental Shelf Oil Controversy
Engineering a blue light inducible SpyCatcher system (BLISS) as a tool for protein photopatterning and optogenetics
The SpyTag-SpyCatcher protein conjugation system has recently exploded in popularity due to its fast kinetics and high yield under biologically favorable conditions in both in vitro and intracellular settings. We imagine we can further expand the utility of this system by introducing the ability to spatially and temporally control the conjugation event. Taking inspiration from photoreceptor proteins in nature, we designed a method to integrate light dependency into the protein conjugation reaction. The light-oxygen-voltage 2 domain of Avena sativa (AsLOV2) undergoes a dramatic conformational change in response to blue light. We have thus genetically fused the SpyTag into the AsLOV2 domain to create a Blue Light Inducible SpyCatcher System (BLISS). In this design (Figure 1), the SpyTag is blocked from reacting with the SpyCatcher in the dark, but upon irradiation with blue light, the Jα-helix of the AsLOV2 undocks to expose the SpyTag. We screened several likely insertion points in the Jα-helix, and found a variant with desirable light switching behavior where after one hour of irradiation, the reaction is 80% complete, while only 10% of the AsLOV2-SpyTag protein reacted in the dark. This reaction can be quenched within minutes by returning the reaction to the dark. We demonstrated the spatial aspect of this light control mechanism through photopatterning proteins onto Ni-NTA coated slides. As our system is made solely from protein components, which can be genetically encoded, we can extend the same spatiotemporal control of proteins inside cells. We anticipate BLISS will be a strong tool for fabricating protein microassays, crafting biomaterial composition, as well as optically controlling enzyme activity and protein localization in cells.
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Doppler cooling of gallium atoms: 2. Simulation in complex multilevel systems
This paper derives a general procedure for the numerical solution of the
Lindblad equations that govern the coherences arising from multicoloured light
interacting with a multilevel system. A systematic approach to finding the
conservative and dissipative terms is derived and applied to the laser cooling
of gallium. An improved numerical method is developed to solve the
time-dependent master equation and results are presented for transient cooling
processes. The method is significantly more robust, efficient and accurate than
the standard method and can be applied to a broad range of atomic and molecular
systems. Radiation pressure forces and the formation of dynamic dark-states are
studied in the gallium isotope 66Ga.Comment: 15 pages, 8 figure
Mending the Levee: How Supernaturally Anchored Conceptions of the Person Impact on Trauma Perception and Healing among Children (Cases from Madagascar and Nepal)
When dealing with children and youth who experience distressing events, psychosocial diagnostics and healing programmes principally resort to biomedical models. Children are often viewed as individualised ‘victims’ suffering from trauma and ‘in need’ of outside help. Highlighting case studies from Madagascar and Nepal, this article argues that the biomedical approach to trauma would be strengthened by a concomitant analysis of social networks, including the perceived relations with the supernatural. The various tandems of family and kin relationships, the living and the dead, constitute not only a social ‘levee’ breached by distressing events, but also the locus around which social relations are rebuilt
Exposure to Trauma and Mental Health Service Engagement Among Adults Who were Children of the Northern Ireland Troubles of 1968 to 1998
Singularities of -fold integrals of the Ising class and the theory of elliptic curves
We introduce some multiple integrals that are expected to have the same
singularities as the singularities of the -particle contributions
to the susceptibility of the square lattice Ising model. We find
the Fuchsian linear differential equation satisfied by these multiple integrals
for and only modulo some primes for and , thus
providing a large set of (possible) new singularities of the . We
discuss the singularity structure for these multiple integrals by solving the
Landau conditions. We find that the singularities of the associated ODEs
identify (up to ) with the leading pinch Landau singularities. The second
remarkable obtained feature is that the singularities of the ODEs associated
with the multiple integrals reduce to the singularities of the ODEs associated
with a {\em finite number of one dimensional integrals}. Among the
singularities found, we underline the fact that the quadratic polynomial
condition , that occurs in the linear differential equation
of , actually corresponds to a remarkable property of selected
elliptic curves, namely the occurrence of complex multiplication. The
interpretation of complex multiplication for elliptic curves as complex fixed
points of the selected generators of the renormalization group, namely
isogenies of elliptic curves, is sketched. Most of the other singularities
occurring in our multiple integrals are not related to complex multiplication
situations, suggesting an interpretation in terms of (motivic) mathematical
structures beyond the theory of elliptic curves.Comment: 39 pages, 7 figure
Anaphylaxis
Anaphylaxis is an acute, potentially fatal systemic reaction with varied mechanisms and clinical presentations. Although prompt recognition and treatment of anaphylaxis are imperative, both patients and healthcare professionals often fail to recognize and diagnose early signs and symptoms of the condition. Clinical manifestations vary widely, however, the most common signs are cutaneous symptoms, including angioedema, urticaria, erythema and pruritus. Immediate intramuscular administration of epinephrine into the lateral thigh is first-line therapy, even if the diagnosis is uncertain. The mainstays of long-term management include specialist assessment, avoidance measures, and the provision of an epinephrine auto-injector and an individualized anaphylaxis action plan. This article provides an overview of the causes, clinical features, diagnosis and acute and long-term management of this serious allergic reaction
El autor piensa en público : David Lodge presenta su novela Pensamientos Ocultos'
Obra ressenyada: David LODGE, Pensamiento ocultosapplication/pd
Predictive processing and the varieties of psychological trauma
A recently popular framework in the cognitive sciences takes the human nervous system to be a hierarchically arranged Bayesian prediction machine. In this paper, we examine psychological trauma through the lens of this framework. We suggest that this can help us to understand the nature of trauma, and the different effects that different kinds of trauma can have. We end by exploring synergies between our approach and current theories of PTSD, and gesture toward future directions
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