Relationships between egg-recognition and egg-ejection in a grasp-ejector species

Brood parasitism frequently leads to a total loss of host fitness, which selects for the evolution of defensive traits in host species. Experimental studies have demonstrated that recognition and rejection of the parasite egg is the most common and efficient defence used by host species. Egg-recognition experiments have advanced our knowledge of the evolutionary and coevolutionary implications of egg recognition and rejection. However, our understanding of the proximate mechanisms underlying both processes remains poor. Egg rejection is a complex behavioural process consisting of three stages: egg recognition, the decision whether or not to reject the putative parasitic egg and the act of ejection itself. We have used the blackbird (Turdus merula) as a model species to explore the relationship between egg recognition and the act of egg ejection. We have manipulated the two main characteristics of parasitic eggs affecting egg ejection in this grasp-ejector species: the degree of colour mimicry (mimetic and non-mimetic, which mainly affects the egg-recognition stage of the egg-rejection process) and egg size (small, medium and large, which affects the decision to eject), while maintaining a control group of non-parasitized nests. The behaviour of the female when confronted with an experimental egg was filmed using a video camera. Our results show that egg touching is an indication of egg recognition and demonstrate that blackbirds recognized (i.e., touched) non-mimetic experimental eggs significantly more than mimetic eggs. However, twenty per cent of the experimental eggs were touched but not subsequently ejected, which confirms that egg recognition does not necessarily mean egg ejection and that accepting parasitic eggs, at least sometimes, is the consequence of acceptance decisions. Regarding proximate mechanisms, our results show that the delay in egg ejection is not only due to recognition problems as usually suggested, given that experimental eggs are not touched significantly more often. Thus, the delay in egg ejection is mainly the consequence of a delay in the decision to eject, probably triggered by mechanical constraints imposed by eggs that are harder to eject (i.e. larger). Our results offer important information on the relationships between recognition and ejection and contribute to a better understanding of host defences against brood parasites.Financial support was provided by the Junta de Andalucía (research project CVI-6653). JDI is funded by a postdoctoral contract (TAHUB-104) from the “Andalucía Talent Hub” program (co-funded by the European's Union Seventh Framework Program Marie Skłodowska-Curie actions (COFUND) and the regional Government of Andalucía)

Could a Factor That Does Not Affect Egg Recognition Influence the Decision of Rejection?

Rejection of the parasitic egg is the most important defence of hosts against brood parasites. However, this response is variable among and within species, and egg discrimination is not always followed by egg rejection. Low risk of parasitism and high risk of rejection costs may lead to the acceptance of the parasitic egg even if it has been previously recognized. The main aim of this paper is to answer a relevant question: can a single egg trait provoke the acceptance of an experimental egg previously recognized as foreign? Increased egg mass should hamper the ejection of an egg that has been discriminated because ejection of a heavy egg may imply higher rejection costs for hosts. We have tested this prediction by experimentally parasitizing natural nests of Common Blackbirds (Turdus merula) with non-mimetic model eggs of different mass (heavy, normal-weight, and light) while controlling for potential confounding factors such as egg size and colour. Our results showed that blackbirds more frequently accepted heavy eggs, even when previously recognized. This differential acceptance may be related to insufficient motivation to assume the higher costs that the ejection of a heavy egg could impose.Financial support has been provided by the Consejería Economía, Innovación, Ciencia y Empleo, Junta de Andalucia (research project CVI-6653)

The filamentary structures in the CO emission toward the Milky Way disk

We present a statistical study of the filamentary structure orientation in the CO emission observations obtained in the Milky Way Imaging Scroll Painting survey in the range 25 (.)degrees . degrees 8 < l < 49 (.)degrees . degrees 7, |b| <= 1 (.)degrees degrees 25, and -100 < v(LSR) < 135 km s(-1). We found that most of the filamentary structures in the (CO)-C-12 and (CO)-C-13 emission do not show a global preferential orientation either parallel or perpendicular to the Galactic plane. However, we found ranges in Galactic longitude and radial velocity where the (CO)-C-12 and (CO)-C-13 filamentary structures are parallel to the Galactic plane. These preferential orientations are different from those found for the HI emission. We consider this an indication that the molecular structures do not simply inherit these properties from parental atomic clouds. Instead, they are shaped by local physical conditions, such as stellar feedback, magnetic fields, and Galactic spiral shocks

The Galactic dynamics revealed by the filamentary structure in atomic hydrogen emission

We present a study of the filamentary structure in the neutral atomic hydrogen (H ̄I) emission at the 21 cm wavelength toward the Galactic plane using the 16 2.2-resolution observations in the H ̄I 4(HI4PI) survey. Using the Hessian matrix method across radial velocity channels, we identified the filamentary structures and quantified their orientations using circular statistics. We found that the regions of the Milky Waya's disk beyond 10 kpc and up to roughly 18 kpc from the Galactic center display H ̄I filamentary structures predominantly parallel to the Galactic plane. For regions at lower Galactocentric radii, we found that the H ̄I filaments are mostly perpendicular or do not have a preferred orientation with respect to the Galactic plane. We interpret these results as the imprint of supernova feedback in the inner Galaxy and Galactic rotation and shear in the outer Milky Way. We found that the H ̄I filamentary structures follow the Galactic warp and flaring and that they highlight some of the variations interpreted as the effect of the gravitational interaction with satellite galaxies. In addition, the mean scale height of the filamentary structures is lower than that sampled by the bulk of the H ̄I emission, thus indicating that the cold and warm atomic hydrogen phases have different scale heights in the outer galaxy. Finally, we found that the fraction of the column density in H ̄I filaments is almost constant up to approximately 18 kpc from the Galactic center. This is possibly a result of the roughly constant ratio between the cold and warm atomic hydrogen phases inferred from the H ̄I absorption studies. Our results indicate that the H ̄I filamentary structures provide insight into the dynamical processes shaping the Galactic disk. Their orientations record how and where the stellar energy input, the Galactic fountain process, the cosmic ray diffusion, and the gas accretion have molded the diffuse interstellar medium in the Galactic plane

The Thermal Design, Characterization, and Performance of the SPIDER Long-Duration Balloon Cryostat

We describe the SPIDER flight cryostat, which is designed to cool six millimeter-wavelength telescopes during an Antarctic long-duration balloon flight. The cryostat, one of the largest to have flown on a stratospheric payload, uses liquid helium-4 to deliver cooling power to stages at 4.2 and 1.6 K. Stainless steel capillaries facilitate a high flow impedance connection between the main liquid helium tank and a smaller superfluid tank, allowing the latter to operate at 1.6 K as long as there is liquid in the 4.2 K main tank. Each telescope houses a closed cycle helium-3 adsorption refrigerator that further cools the focal planes down to 300 mK. Liquid helium vapor from the main tank is routed through heat exchangers that cool radiation shields, providing negative thermal feedback. The system performed successfully during a 17 day flight in the 2014-2015 Antarctic summer. The cryostat had a total hold time of 16.8 days, with 15.9 days occurring during flight.Comment: 15 pgs, 17 fig

Radio continuum emission in the northern Galactic plane: Sources and spectral indices from the THOR survey

Radio continuum surveys of the Galactic plane can find and characterize HII regions, supernova remnants (SNRs), planetary nebulae (PNe), and extragalactic sources. A number of surveys at high angular resolution (<25") at different wavelengths exist to study the interstellar medium (ISM), but no comparable high-resolution and high-sensitivity survey exists at long radio wavelengths around 21cm. We observed a large fraction of the Galactic plane in the first quadrant of the Milky Way (l=14.0-67.4deg and |b| < 1.25deg) with the Karl G. Jansky Very Large Array (VLA) in the C-configuration covering six continuum spectral windows. These data provide a detailed view on the compact as well as extended radio emission of our Galaxy and thousands of extragalactic background sources. We used the BLOBCAT software and extracted 10916 sources. After removing spurious source detections caused by the sidelobes of the synthesised beam, we classified 10387 sources as reliable detections. We smoothed the images to a common resolution of 25" and extracted the peak flux density of each source in each spectral window (SPW) to determine the spectral indices $\alpha$ (assuming $I(\nu)\propto\nu^\alpha$). By cross-matching with catalogs of HII regions, SNRs, PNe, and pulsars, we found radio counterparts for 840 HII regions, 52 SNRs, 164 PNe, and 38 pulsars. We found 79 continuum sources that are associated with X-ray sources. We identified 699 ultra-steep spectral sources ($\alpha < -1.3$) that could be high-redshift galaxies. Around 9000 of the sources we extracted are not classified specifically, but based on their spatial and spectral distribution, a large fraction of them is likely to be extragalactic background sources. More than 7750 sources do not have counterparts in the SIMBAD database, and more than 3760 sources do not have counterparts in the NED database

Environmental and Parental Influences on Offspring Health and Growth in Great Tits (Parus major)

PMCID: PMC3728352This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

The history of dynamics and stellar feedback revealed by the HI filamentary structure in the disk of the Milky Way

We present a study of the filamentary structure in the emission from the neutral atomic hydrogen (HI) at 21 cm across velocity channels in the 40 '' and 1.5-km s(-1) resolution position-position-velocity cube, resulting from the combination of the single-dish and interferometric observations in The HI/OH/recombination-line survey of the inner Milky Way. Using the Hessian matrix method in combination with tools from circular statistics, we find that the majority of the filamentary structures in the HI emission are aligned with the Galactic plane. Part of this trend can be assigned to long filamentary structures that are coherent across several velocity channels. However, we also find ranges of Galactic longitude and radial velocity where the HI filamentary structures are preferentially oriented perpendicular to the Galactic plane. These are located (i) around the tangent point of the Scutum spiral arm and the terminal velocities of the Molecular Ring, around l approximate to 28 degrees and v(LSR) approximate to 100 km s(-1), (ii) toward l approximate to 45 degrees and v(LSR) approximate to 50 km s(-1), (iii) around the Riegel-Crutcher cloud, and (iv) toward the positive and negative terminal velocities. A comparison with numerical simulations indicates that the prevalence of horizontal filamentary structures is most likely the result of large-scale Galactic dynamics and that vertical structures identified in (i) and (ii) may arise from the combined effect of supernova (SN) feedback and strong magnetic fields. The vertical filamentary structures in (iv) can be related to the presence of clouds from extra-planar HI gas falling back into the Galactic plane after being expelled by SNe. Our results indicate that a systematic characterization of the emission morphology toward the Galactic plane provides an unexplored link between the observations and the dynamical behavior of the interstellar medium, from the effect of large-scale Galactic dynamics to the Galactic fountains driven by SNe

Egg rejection in blackbirds Turdus merula: a by-product of conspecific parasitism or successful resistance against interspecific brood parasites?

Traditional theory assumes that egg recognition and rejection abilities arise as a response against interspecific brood parasitism (IBP). However, rejection also appears in some species that are currently not exploited by interspecific parasites, such as Turdus thrushes. Recent evidences suggest that rejection abilities evolved in these species as a response to conspecific brood parasitism (CBP). To test these two alternative hypotheses, we performed an experimental study by parasitizing nests of the common blackbird (Turdus merula) with conspecifics or heterospecific eggs under different risk of parasitism (presence of interspecific or conspecific parasites near the nest). Common blackbird is a potential host of the common cuckoo (Cuculus canorus) but suffers low levels of CBP too. Results: We found that blackbirds were able to recognize and eject heterospecific eggs at high rates whereas most of conspecifics eggs were not recognized and, therefore, accepted. Ejection rates of conspecific eggs did not exceed 13 %, even in situations of high risk of CBP (blackbird female placed near the nest), which contradict the main prediction derived from the CBP hypothesis. Conversely, ejection rates of experimental eggs simulating IBP were much higher (80–100 %). Furthermore, female blackbirds were more aggressive towards cuckoos than towards blackbird dummies. Conclusions: Our results considered together support the IBP hypothesis, indicating that recognition and rejection of parasitic eggs in blackbirds have probably evolved due to previous cuckoo parasitism. The current absence of IBP in blackbirds may be due to the highly efficient rejection abilities in this species. Thus, these abilities have been retained in absence of brood parasitism as a consequence of the low costs involved for blackbirds, resulting in a successful resistance against interspecific brood parasitism.Financial support has been provided by the Consejería Economía, Innovación, Ciencia y Empleo. Junta de Andalucia (research project CVI-6653)