Visualizing polymeric components that define distinct root barriers across plant lineages

Hydrophobic cell wall depositions in roots play a key role in plant development and interaction with the soil environment, as they generate barriers that regulate bidirectional nutrient flux. Techniques to label the respective polymers are emerging, but are efficient only in thin roots or sections. Moreover, simultaneous imaging of the barrier constituents lignin and suberin remains problematic owing to their similar chemical compositions. Here, we describe a staining method compatible with single- and multiphoton confocal microscopy that allows for concurrent visualization of primary cell walls and distinct secondary depositions in one workflow. This protocol permits efficient separation of suberin- and lignin-specific signals with high resolution, enabling precise dissection of barrier constituents. Our approach is compatible with imaging of fluorescent proteins, and can thus complement genetic markers or aid the dissection of barriers in biotic root interactions. We further demonstrate applicability in deep root tissues of plant models and crops across phylogenetic lineages. Our optimized toolset will significantly advance our understanding of root barrier dynamics and function, and of their role in plant interactions with the rhizospheric environment

Development of an Understanding of Reactive Mercury in Ambient Air: A Review

This review focuses on providing the history of measurement efforts to quantify and characterize the compounds of reactive mercury (RM), and the current status of measurement methods and knowledge. RM collectively represents gaseous oxidized mercury (GOM) and that bound to particles. The presence of RM was first recognized through measurement of coal-fired power plant emissions. Once discovered, researchers focused on developing methods for measuring RM in ambient air. First, tubular KCl-coated denuders were used for stack gas measurements, followed by mist chambers and annular denuders for ambient air measurements. For ~15 years, thermal desorption of an annular KCl denuder in the Tekran® speciation system was thought to be the gold standard for ambient GOM measurements. Research over the past ~10 years has shown that the KCl denuder does not collect GOM compounds with equal efficiency, and there are interferences with collection. Using a membrane-based system and an automated system—the Detector for Oxidized mercury System (DOHGS)—concentrations measured with the KCl denuder in the Tekran speciation system underestimate GOM concentrations by 1.3 to 13 times. Using nylon membranes it has been demonstrated that GOM/RM chemistry varies across space and time, and that this depends on the oxidant chemistry of the air. Future work should focus on development of better surfaces for collecting GOM/RM compounds, analytical methods to characterize GOM/RM chemistry, and high-resolution, calibrated measurement systems

Determining sources of reactive mercury compounds in Reno, Nevada, United States

There is much uncertainty regarding the sources of reactive mercury (RM) compounds and atmospheric chemistry driving their formation. This work focused on assessing the chemistry and potential sources of reactive mercury measured in Reno, Nevada, United States, using 1 year of data collected using Reactive Mercury Active System. In addition, ancillary meteorology and criteria air pollutant data, Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) analyses, and a generalized linear model were applied to better understand reactive mercury observations. During the year of sampling, a fire event impacted the sampling site, and gaseous elemental Hg and particulate-bound mercury concentrations increased, as did HgII-S compounds. Data collected on a peak above Reno showed that reactive mercury concentrations were higher at higher elevation, and compounds found in Reno were the same as those measured on the peak. HYSPLIT results demonstrated RM compounds were generated inside and outside of the basin housing Reno. Compounds were sourced from San Francisco, Sacramento, and Reno in the fall and winter, and from long-range transport and the marine boundary layer during the spring and summer. The generalized linear model produced correlations that could be explained; however, when applying the model to similar data collected at two other locations, the Reno model did not predict the observations, suggesting that sampling location chemistry and concentration cannot be generalized

Performance of the Beetle readout chip for LHCb

Beetle is a 128-channel readout chip, which will be used in the silicon vertex detector, the pile-up veto counters and the silicon tracker of the LHCb experiment at CERN. A further application of the Beetle chip is the readout of the LHCb RICH, in case it is equipped with multi-anode PMTs. The scope of this paper is the design changes leading to the latest version 1.3 of the Beetle readout chip. In addition, measurements on earlier versions and simulation results driving these changes are shown

SEU Robustness, Total Dose Radiation Hardness and Analogue Performance of the Beetle Chip

The Beetle is a 128 channel readout chip for silicon strip detectors in LHCb. In addition to the pipelined readout path known from the RD20 architecture which can be used either in analogue or binary mode, the Beetle features an additional prompt binary readout path, used for the LHCb pile-up veto counters and a triple-redundant layout of the control logic. It is manufactured in commercial 0.25 µm CMOS technology using radiation hard design techniques. In addition to a total dose irradiation with X-rays, an SEU irradiation test with 65 MeV protons was performed with Beetle1.3. The results of this test are presented together with new results from the Beetle versions 1.3, 1.4 and 1.5, which were submitted in the Beetle ER engineering run in May 2004

Enhanced Radiation Hardness and Faster Front Ends for the Beetle Readout Chip

This paper summarizes the recent progress in the development of the 128 channel pipelined readout chip Beetle, which is intended for the silicon vertex detector, the inner tracker, the pile-up veto trigger and the RICH detectors of LHCb. Deficiencies found in the front end of the Beetle Version 1.0 and 1.1 chips resulted in the submissions of BeetleFE 1.1 and BeetleFE 1.2, while BeetleSR 1.0 implements test circuits to provide future Beetle chips with logic circuits hardened against single event upset (SEU). Section I. motivates the development of new front ends for the Beetle chip, and section II. summarizes their concepts and construction. Section III. reports preliminary results from the BeetleFE 1.1 and BeetleFE 1.2 chips, while section IV. describes the BeetleSR 1.0 chip. An outlook on future test and development of the Beetle chip is given in section V

Inclusive V0V^0 Production Cross Sections from 920 GeV Fixed Target Proton-Nucleus Collisions

Inclusive differential cross sections $d\sigma_{pA}/dx_F$ and $d\sigma_{pA}/dp_t^2$ for the production of \kzeros, \lambdazero, and \antilambda particles are measured at HERA in proton-induced reactions on C, Al, Ti, and W targets. The incident beam energy is 920 GeV, corresponding to $\sqrt {s} = 41.6$ GeV in the proton-nucleon system. The ratios of differential cross sections \rklpa and \rllpa are measured to be $6.2\pm 0.5$ and $0.66\pm 0.07$, respectively, for \xf $\approx-0.06$. No significant dependence upon the target material is observed. Within errors, the slopes of the transverse momentum distributions $d\sigma_{pA}/dp_t^2$ also show no significant dependence upon the target material. The dependence of the extrapolated total cross sections $\sigma_{pA}$ on the atomic mass $A$ of the target material is discussed, and the deduced cross sections per nucleon $\sigma_{pN}$ are compared with results obtained at other energies.Comment: 17 pages, 7 figures, 5 table

The QCD transition temperature: results with physical masses in the continuum limit II.

We extend our previous study [Phys. Lett. B643 (2006) 46] of the cross-over temperatures (T_c) of QCD. We improve our zero temperature analysis by using physical quark masses and finer lattices. In addition to the kaon decay constant used for scale setting we determine four quantities (masses of the \Omega baryon, K^*(892) and \phi(1020) mesons and the pion decay constant) which are found to agree with experiment. This implies that --independently of which of these quantities is used to set the overall scale-- the same results are obtained within a few percent. At finite temperature we use finer lattices down to a <= 0.1 fm (N_t=12 and N_t=16 at one point). Our new results confirm completely our previous findings. We compare the results with those of the 'hotQCD' collaboration.Comment: 19 pages, 8 figures, 3 table