Physical activity and lung function association in a healthy community-dwelling European population.

The association of physical activity (PA) and lung function (LF) varies from no measurable effect to delayed LF decline. We assessed the association between accelerometery-assessed PA and LF in a sample of apparently healthy, community-dwelling subjects. We included two cross-sectional studies using data from the PneumoLaus study (2014-17 and 2018-21), conducted in Lausanne, Switzerland. PA was assessed by accelerometry and categorised as inactivity, light, moderate or vigorous. Forced expiratory volume in 1 second (FEV <sub>1</sub> ), forced volume capacity (FVC) and maximal mid-expiratory flow (MMEF) were measured by spirometry and expressed in percentage of predicted value (PV). Overall, 1'910 (54.7% women, 62.0 ± 9.7 years) and 1'174 (53.4% women, 65.8 ± 9.5 years) participants were included in the first and the second surveys, respectively. In both surveys, moderate and vigorous PA showed a weak but significant correlation with FEV <sub>1</sub> in percentage (PV) (R = 0.106 and 0.132 for the first and 0.111 and 0.125 for the second surveys, p < 0.001). Similar correlations with FVC (p < 0.001) were found. Associations held irrespective of smoking status and remained after multivariable adjustment. Fewer associations were detected between LF and light PA or between MMEF and PA. Moderate and vigorous intensity PA are associated with increased LF regardless of smoking status in apparently healthy community-dwelling European population. These associations are statistically but not clinically significant due to the small correlation coefficients (R < 0.30), corresponding to a weak association

Prevalence and whole genome-based phylogenetic, virulence and antibiotic ­resistance characteristics of nasal ­Staphylococcus aureus in healthy Swiss horses

A total of 100 nasal swabs were collected from healthy horses in Switzerland between January 2020 and August 2020. The samples were taken from horses at 40 different stables in 12 different cantons and screened for both methicillin-resistant (MRSA) and methicillin-susceptible S. aureus (MSSA) using selective agar plates. S. aureus were tested for antibiotic susceptibility by measurement of the minimal inhibitory concentration (MIC) and for virulence factors, antibiotic resistance genes and phylogenetic characteristics using whole genome sequence analysis. Ten horses were found to be positive (10 %, CI: 95 %, 0,0552 - 0,1744) for S. aureus, and four of them harboured MRSA (4 %, CI: 95 %, CI: 1,5 % - 9 %). The MRSA were detected in horses from three different stables in the same region of one canton and MSSA were detected in horses from five different cantons. All the MRSA isolates were genetically related (ST398-t011-IVa), while the MSSA were diverse (ST1-t127/t398/t1508, ST816-t1294, ST133-t1403, ST30-t012). MRSA showed resistance to penicillin (blaZ), cefoxitin (mecA), trimethoprim (dfrK), gentamicin, kanamycin (aac(6')-Ie - aph(2'')-Ia), and tetracycline (tet(M)). MSSA were resistant to either none or one of the antibiotics tested like penicillin (blaZ) and erythromycin (erm(T)). Virulence genes were more abundant in MSSA than in MRSA. This study provides first insight into the prevalence and type of S. aureus in healthy Swiss horses and reveals a source of strains, which may cause infections in both horses and humans

Distribution, genetic heterogeneity, and antimicrobial susceptibility of Brachyspira pilosicoli in Swiss pig herds

Brachyspira (B.) pilosicoli is a bacterium causing porcine intestinal spirochaetosis, a disease characterized by diarrhoea and depressed growth rates especially in nursery and fattening pigs. Knowledge of the epidemiology and antimicrobial susceptibility of this pathogen is limited. Therefore, the aim of this study was to analyse the distribution, genetic heterogeneity, and antimicrobial susceptibility of B. pilosicoli field isolates from Swiss pig farms. Faecal swabs of 693 animals originating from 156 herds were analysed for the presence of Brachyspira spp. using culture and polymerase chain reaction identification. Further characterisation was performed using multilocus sequence typing (MLST) and broth dilution antimicrobial susceptibility testing. With 52.6% positive herds, B. pilosicoli could be frequently isolated from herds with animals suffering from diarrhoea. In herds with animals without clinical signs of diarrhoea, detection was significantly less frequent with only 10.5% positive herds (p 0.001). Among 80 isolates used for typing, genetic heterogeneity was observed with 44 different sequence types (ST) which often differed from herd to herd. No predominant ST was observed. More than 73.0% of the 41 B. pilosicoli isolates analysed, showed minimal inhibitory concentration values above the wild type cut-off values for lincomycin, tylvalosin and/ or tylosin. For tiamulin, valnemulin and doxycycline, this was the case in 48.8%, 43.9% and 36.6%, respectively. In conclusion, a diverse population of B. pilosicoli exhibited decreased susceptibility to antimicrobials used against Brachyspira infections. Monitoring of resistance in Brachyspira spp. is highly recommended to support targeted use of antimicrobials in pigs

Saharan dust events at the Jungfraujoch: detection by wavelength dependence of the single scattering albedo and first climatology analysis

International audienceScattering and absorption coefficients have been measured continuously at several wavelengths since March 2001 at the high altitude site Jungfraujoch (3580ma.s.l.). From these data, the wavelength dependences of the Ångström exponent and particularly of the single scattering albedo are determined. While the exponent of the single scattering albedo usually increases with wavelength, it decreases with wavelength during Saharan dust events (SDE) due to the greater size of the mineral aerosol particles and their different chemical composition. This change in the sign of the single scattering exponent turns out to be a sensitive means for detecting Saharan dust events. The occurrence of SDE detected by this new method was confirmed by visual inspection of filter colors and by studying long-range back-trajectories. An examination of SDE over a 22-month period shows that SDE are more frequent during the March-June period as well as during October and November. The trajectory analysis indicated a mean traveling time of 96.5h, with the most important source countries situated in the northern and north-western part of the Saharan desert. Most of the SDE do not lead to a detectable increase of the 48-h total suspended particulate matter (TSP) concentration at the Jungfraujoch. During Saharan dust events, the average contribution of this dust to hourly TSP at the Jungfraujoch is 16µg/m3, which corresponds to an annual mean of 0.8µg/m3 or 24% of TSP

Design standards for icons: The independent role of aesthetics, visual complexity and concreteness in icon design and icon understanding

Icons play an important role in modern interfaces and therefore recent empirical research has focused on enhancing icon processing — that is, icon perception and icon function understanding. However, in existing sets, icons vary simultaneously across different icon characteristics, confusing the contribution of each to icon processing. We developed icon design principles for aesthetics, complexity, and concreteness, and used them to create 64 icons that varied independently along each characteristic. Participants reported the icon function and rated each icon in terms of aesthetics, complexity and concreteness. The manipulated characteristics had independent effects on icon processing, with two exceptions, for which we propose evidence-based solutions. Based on these findings we propose guidelines for designing icons for research purposes

Driving factors of aerosol properties over the foothills of central Himalayas based on 8.5 years continuous measurements

This study presents analysis of in situ measurements conducted over the period 2005–2014 in the Indian Himalayas to give a thorough overview of the factors and causes that drive aerosol properties. Aerosol extensive properties (namely, particle number concentration, scattering coefficient, equivalent black carbon, PM2.5, and PM10) have 1.5–2 times higher values in the early to late afternoon than during the night, and a strong seasonality. The interannual variability is ±20% for both PM2.5 and total particle number concentration. Analysis of the data shows statistically significant decreasing trends of −2.3 μg m−3 year−1 and −2.7 μg m−3 year−1 for PM2.5 and PM10, respectively, over the study period. The mountainous terrain site (Mukteshwar, MUK) is primarily under the influence of air from the plains. This is due to convective transport processes that are enhanced by local and mesoscale topography, leading to pronounced valley/mountain winds and consequently to atmospheric boundary layer air lifting from the plains below. The transport from plains is evident in seasonal‐diurnal patterns observed at MUK. The timing of the patterns corresponds with changes in turbulence and water vapor (q). According to our analysis, using these as proxies is a viable method for examining boundary layer influence in the absence of direct atmospheric boundary layer height measurements. Comparing the measurements with climate models shows that even regional climate models have problems capturing the orographic influence accurately at MUK, highlighting the importance of long‐term direct measurements at multiple points to understand aerosol behavior in mountainous areas

Low occurrence of Brachyspira hyodysenteriae in Swiss pig herds with diarrhoea.

In the Swiss pig population, only four sequence types (ST6, ST66, ST196, ST197) of Brachyspira ( B .) hyodysenteriae , an agent of Swine Dysentery (SD), have been so far detected suggesting a limited number of sources of B. hyodysenteriae in the Swiss pig production. A one year culture- and molecular-based diagnostic project was performed to identify and trace back B. hyodysenteriae in pig herds with SD, and to identify possible new STs. Up to five faecal swabs from herds with diarrhoea were examined. Three out of 141 herds tested positive for B. hyodysenteriae of ST196 (n=2) and ST66 (n=1). A common source was unlikely as none of the supplier herds or pig trader was shared and the occurrence was very low (2.1%) in the study population. This low occurrence may have resulted from the monitoring and eradications during the last ten years, emphasising their further applications to control B. hyodysenteriae

Characterization and intercomparison of aerosol absorption photometers: Result of two intercomparison workshops

Absorption photometers for real time application have been available since the 1980s, but the use of filter-based instruments to derive information on aerosol properties (absorption coefficient and black carbon, BC) is still a matter of debate. Several workshops have been conducted to investigate the performance of individual instruments over the intervening years. Two workshops with large sets of aerosol absorption photometers were conducted in 2005 and 2007. The data from these instruments were corrected using existing methods before further analysis. The inter-comparison shows a large variation between the responses to absorbing aerosol particles for different types of instruments. The unit to unit variability between instruments can be up to 30% for Particle Soot Absorption Photometers (PSAPs) and Aethalometers. Multi Angle Absorption Photometers (MAAPs) showed a variability of less than 5%. Reasons for the high variability were identified to be variations in sample flow and spot size. It was observed that different flow rates influence system performance with respect to response to absorption and instrumental noise. Measurements with non absorbing particles showed that the current corrections of a cross sensitivity to particle scattering are not sufficient. Remaining cross sensitivities were found to be a function of the total particle load on the filter. The large variation between the response to absorbing aerosol particles for different types of instruments indicates that current correction functions for absorption photometers are not adequate

Early neoplastic and metastatic mammary tumours of transgenic mice detected by 5-aminolevulinic acid-stimulated protoporphyrin IX accumulation

A photodynamic technique for human breast cancer detection founded upon the ability of tumour cells to rapidly accumulate the fluorescent product protoporphyrin IX (PpIX) has been applied to transgenic mouse models of mammary tumorigenesis. A major goal of this investigation was to determine whether mouse mammary tumours are reliable models of human disease in terms of PpIX accumulation, for future mechanistic and therapeutic studies. The haeme substrate 5-aminolevulinic acid (5-ALA) (200 mg kg−1) was administered to mouse strains that develop mammary tumours of various histological subtypes upon expression of the transgenic oncogenes HRAS, Polyoma Virus middle T antigen, or Simian Virus 40 large T antigen in the mammary gland. Early neoplastic lesions, primary tumours and metastases showed consistent and rapid PpIX accumulation compared to the normal surrounding tissues, as evidenced by red fluorescence (635 nm) when the tumours were directly illuminated with blue light (380–440 nm). Detection of mouse mammary tumours at the stage of ductal carcinoma in situ by red fluorescence emissions suggests that enhanced PpIX synthesis is a good marker for early tumorigenic processes in the mammary gland. We propose the mouse models provide an ideal experimental system for further investigation of the early diagnostic and therapeutic potential of 5-ALA-stimulated PpIX accumulation in human breast cancer patients