98 research outputs found
Projecte d'adequació d'edifici hoteler a petits apartaments turístics a la població de Canet de Mar
d’un edifici del S. XVIII, d’inici construït per a un ús residencial, i que en l’actualitat realitza
l’activitat d’hostal-residència.
La nova activitat que es proposa, be donada pel patrimoni familiar del propietari de l’edifici, ja
que la finca és el resultat d’una composició de diferents edificacions residencials unides entre
sí en la dècada dels seixanta, formant el que va ser un negoci familiar (actualment deshabitat,
excepte els mesos de juliol i agost). La proposta és realitzar un tipus de vivenda-apartament
per a lloguer, amb l’objectiu de reactivar el negoci anomenat i no recórrer a la venta del mateix.
En una primera instància, s’ha realitzat una diagnosi de l’edifici per tal de conèixer l’estat de
conservació, ja que cal anotar que és un edifici que té més de 100 anys. En el transcurs
d’aquesta diagnosi, concretament en la recerca d’informació per conèixer les adicions,
remuntes i tota mena de variacions que ha patit des de l’estat inicial, ens hem endinsat en la
tipologia de la casa tradicional dels pobles costaners, més en concret en la casa denominada
“casa de cos”. Per aquest motiu, s’ha cregut convenient realitzar un breu estudi d’aquesta
tipologia d’edificis i per l’altre banda, realitzar un estudi el més acurat possible de la història del
“nostre” habitacle per poder realitzar una diagnosi el més concreta possible, és a dir, conèixer
els processos constructius que es van emprar en la realització dels fonaments, l’estructura, es
tancaments, les cobertes, etc., ja que la presa de dades no era tot el correcte que es desitjava
per la impossibilitat de desmuntar fals sostres, realitzar cales, enretirar paviments i altres
tipologies de feines necessàries per realitzar la diagnosi.
Un cop finalitzada la recerca de la història del edifici en estudi, es passa a l’anàlisi concret
d’aquest, observant el tipus de lesions i/o patologies existents a causa de la unió d’edificacions
i modificacions de la seva volumetria sense estudis previs que indiquin que els canvis soferts
o, simplement, pel transcurs del temps, els quals, no hagin fet malbé la capacitat funcional de
l’edifici d’estudi.
Un cop finalitzada la diagnosi i realitzades les conclusions, s’ha passat a realitzar el projecte
d’adequació d’edifici hoteler a petits apartaments turístics. L’adequació de l’edifici s’ha realitzat
tal i com indiquen les normatives metropolitanes del municipi, aplicant els materials amb el
menor impacte possible intentant realitzar una rehabilitació el més sostenible possible i
realitzant el màxim nombre d’apartaments per un major rendibilitat. En el projecte d’adequació
s’ha inclòs un petit pla de manteniment per tal de prolongar la seva vida útil i per evitar
possibles lesions futures i una comparativa dels consum de CO2 en la rehabilitació projectada
envers a rehabilitacions comunes.
Per resumir breument l’edifici, es poden destacar les següents peculiaritats:
La primera peculiaritat és l’estructura. Es troba construïda en dos èpoques diferents, utilitzant
els medis característics, els materials i els sistemes constrictius de cadascuna d’elles. D’una
primera època, es poden apreciar volums executats amb forjats amb biguetes de fusta units
amb el pas del temps, amb edificacions construïdes en els anys 50-60, amb forjats biguetes de
formigó armat.
La segona peculiaritat la trobem en la seva façana principal, originaria del s.XVIII i actualment
catalogada com a d’interès pel departament arquitectònic de l’Ajuntament de Canet de Mar, i
que com especifiquem, gràcies a la informació recollida en la investigació de l’evolució de
l’edifici, no es la original ja que es va traslladar uns metres per una expropiació realitzada en
els anys 40. Aquest ha estat un tema a tindre en conte, ja que les distribucions han vingut
determinades per les obertures existents en façana
Módulo de identificación de pasos o situaciones (MIPS)
Herramienta de experto para ayudar a la identificación y selección de pasos y situaciones basándose en las propiedades y observaciones capturadas durante la fase de observación
Caracterización de las proteínas del virus de la fiebre aftosa implicadas en respuesta a mutagénesis letal por análogos de nucleótido
Tesis doctoral inédita. Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 19-06-0
Unravelling plant diversification: Intraspecific genetic differentiation in hybridizing Anacyclus species in the western Mediterranean Basin
Premise: The interfertile species Anacyclus clavatus, A. homogamos, and A. valentinus represent a plant complex coexisting in large anthropic areas of the western Mediterranean Basin with phenotypically mixed populations exhibiting a great floral variation. The goal of this study was to estimate the genetic identity of each species, to infer the role of hybridization in the observed phenotypic diversity, and to explore the effect of climate on the geographic distribution of species and genetic clusters. Methods: We used eight nuclear microsatellites to genotype 585 individuals from 31 populations of three Anacyclus species for population genetic analyses by using clustering algorithms based on Bayesian models and ordination methods. In addition, we used ecological niche models and niche overlap analyses for both the species and genetic clusters. We used an expanded data set, including 721 individuals from 129 populations for ecological niche models of the genetic clusters. Results: We found a clear correspondence between species and genetic clusters, except for A. clavatus that included up to three genetic clusters. We detected individuals with admixed genetic ancestry in A. clavatus and in mixed populations. Ecological niche models predicted similar distributions for species and genetic clusters. For the two specific genetic clusters of A. clavatus, ecological niche models predicted remarkably different areas. Conclusions: Gene flow between Anacyclus species likely explains phenotypic diversity in contact areas. In addition, we suggest that introgression could be involved in the origin of one of the two A. clavatus genetic clusters, which also showed ecological differentiationPID2019‐104135GB‐I00, PID2021‐124187NB‐I0
No sólo importa el tamaño: la morfología del aquenio afecta al tiempo de emergencia de la plántula en tres especies heterocárpicas de Anacyclus (Anthemideae, Asteraceae)
The production of two or more distinct fruit types by an individual, i.e. heterocarpy, is considered as a mixed dispersal strategy in which a proportion of the offspring is able to colonize new sites, whilst others remain near the maternal location. Here, we aimed to explore the effects of achene morphology (winged vs. unwinged achenes) and achene size –measured here as achene mass– on post-dispersal life-history traits (probability and time of seedling emergence) in three heterocarpic Anacyclus species (Anthemideae, Asteraceae). Morphology, size and germination performance were studied in achenes from six populations of Anacyclus clavatus (Desf.) Pers., A. homogamos (Maire) Humphries, and A. valentinus L. Our results show that achene morphology and size were related to their position within the capitulum, such that outer, winged achenes were significantly heavier than the inner, unwinged ones. Additionally, winged achenes germinated faster than unwinged ones. This pattern may be related to the sequential achene time of release displayed by these species. Finally, our findings cast doubt on the role of wings as structures that favor dispersal by wind in these three species of Anacyclus.La producción de dos o más tipos de frutos diferentes por un mismo individuo, i.e. heterocarpia, es considerada como una estrategia mixta de dispersión en la que una parte de la descendencia es capaz de colonizar nuevos sitios, mientras que la otra permanece cerca de la planta madre. En este trabajo, nuestro objetivo fue explorar los efectos de la morfología del aquenio (aquenios alados vs. no alados) y de su tamaño –medido aquí como masa del aquenio– en la etapa del ciclo de vida siguiente a la dispersión (probabilidad de germinación y tiempo de emergencia de las plántulas) de tres especies heterocárpicas del género Anacyclus (Anthemideae, Asteraceae). Se estudió la morfología, el tamaño y la germinación en aquenios de seis poblaciones de Anacyclus clavatus (Desf.) Pers., A. homogamos (Maire) Humphries y A. valentinus L. Nuestros resultados indican que tanto la morfología del aquenio como su tamaño estaban relacionados con su posición en el capítulo, de manera que los aquenios más externos –alados– eran significativamente más pesados que los internos –sin alas–. Además, los aquenios alados germinaron más rápidamente que los no alados. Este patrón puede estar relacionado con la liberación secuencial de los aquenios que ocurre en estas especies. Por último, nuestros resultados ponen en duda la función de las alas como estructuras que favorecen la dispersión por viento de los aquenios en estas tres especies de Anacyclus
Methionine cycle rewiring by targeting miR-873-5p modulates ammonia metabolism to protect the liver from acetaminophen
Drug-induced liver injury (DILI) development is commonly associated with acetaminophen (APAP) overdose, where glutathione scavenging leads to mitochondrial dysfunction and hepatocyte death. DILI is a severe disorder without effective late-stage treatment, since N-acetyl cysteine must be administered 8 h after overdose to be efficient. Ammonia homeostasis is altered during liver diseases and, during DILI, it is accompanied by decreased glycine N-methyltransferase (GNMT) expression and S-adenosylmethionine (AdoMet) levels that suggest a reduced methionine cycle. Anti-miR-873-5p treatment prevents cell death in primary hepatocytes and the appearance of necrotic areas in liver from APAP-administered mice. In our study, we demonstrate a GNMT and methionine cycle activity restoration by the anti-miR-873-5p that reduces mitochondrial dysfunction and oxidative stress. The lack of hyperammoniemia caused by the therapy results in a decreased urea cycle, enhancing the synthesis of polyamines from ornithine and AdoMet and thus impacting the observed recovery of mitochondria and hepatocyte proliferation for regeneration. In summary, anti-miR-873-5p appears to be an effective therapy against APAP-induced liver injury, where the restoration of GNMT and the methionine cycle may prevent mitochondrial dysfunction while activating hepatocyte proliferative response
The structure of a protein primer-polymerase complex in the initiation of genome replication
Picornavirus RNA replication is initiated by the covalent attachment of a UMP molecule to the hydroxyl group of a tyrosine in the terminal protein VPg. This reaction is carried out by the viral RNA-dependent RNA polymerase (3D). Here, we report the X-ray structure of two complexes between foot-and-mouth disease virus 3D, VPg1, the substrate UTP and divalent cations, in the absence and in the presence of an oligoadenylate of 10 residues. In both complexes, VPg fits the RNA binding cleft of the polymerase and projects the key residue Tyr3 into the active site of 3D. This is achieved by multiple interactions with residues of motif F and helix α8 of the fingers domain and helix α13 of the thumb domain of the polymerase. The complex obtained in the presence of the oligoadenylate showed the product of the VPg uridylylation (VPg-UMP). Two metal ions and the catalytic aspartic acids of the polymerase active site, together with the basic residues of motif F, have been identified as participating in the priming reaction. © 2006 European Molecular Biology Organization | All Rights Reserved.Work in Barcelona was supported by Grants BIO2002-00517 and BFU2005-02376/BMC. Work in Madrid by Grants BMC 2001.1823.C02-01, BFU2005-00863/BMC and Fundación R Areces. CF and AA were supported by I3P fellowships from Ministerio de Educación y Ciencia. RA was supported by an FPI fellowship from Comunidad de Madrid. The financial support was provided by the ESRFPeer Reviewe
Power rating based on two different spectroheliometers with lattice-matched (LM) and upright metamorphic (UMM) component solar cells
Synthetic and empirical data have been used to explore the influence of spectral mismatch between MJ cell technologies on outdoor CPV module rating uncertainty. Calibration biases are attenuated by tightly filtering spectral conditions to a spectral matching ratio (SMR) of 1 ± 2.5%. The sensitivity of calibrated current to spectral deviations greatly depends on the direction and distribution of the deviations on the SMR space
Topology of evolving, mutagenized viral populations: quasispecies expansion, compression, and operation of negative selection
Additional files:
Additional file 1: Neighbour-joining, maximum likelihood and maximum parsimony analysis of populations RAp35 and RA0p35.
Additional file 2: Maximum likelihood phylogenetic analysis of mutagenized populations of FMDV.
Additional file 3: Standard errors.Background
The molecular events and evolutionary forces underlying lethal mutagenesis of virus (or virus extinction through an excess of mutations) are not well understood. Here we apply for the first time phylogenetic methods and Partition Analysis of Quasispecies (PAQ) to monitor genetic distances and intra-population structures of mutant spectra of foot-and-mouth disease virus (FMDV) quasispecies subjected to mutagenesis by base and nucleoside analogues.
Results
Phylogenetic and PAQ analyses have revealed a highly dynamic variation of intrapopulation diversity of FMDV quasispecies. The population diversity first suffers striking expansions in the presence of mutagens and then compressions either when the presence of the mutagenic analogue was discontinued or when a mutation that decreased sensitivity to a mutagen was selected. The pattern of mutations found in the populations was in agreement with the behavior of the corresponding nucleotide analogues with FMDV in vitro. Mutations accumulated at preferred genomic sites, and dn/ds ratios indicate the operation of negative (or purifying) selection in populations subjected to mutagenesis. No evidence of unusually elevated genetic distances has been obtained for FMDV populations approaching extinction.
Conclusion
Phylogenetic and PAQ analysis provide adequate procedures to describe the evolution of viral sequences subjected to lethal mutagenesis. These methods define the changes of intra-population structure more precisely than mutation frequencies and Shannon entropies. PAQ is very sensitive to variations of intrapopulation genetic distances. Strong negative (or purifying) selection operates in FMDV populations subjected to enhanced mutagenesis. The quantifications provide evidence that extinction does not imply unusual increases of intrapopulation complexity, in support of the lethal defection model of virus extinction.Work at Centro de Biología Molecular "Severo Ochoa" was supported by grants BFU2006-00863 from MEC, 36558/06 from FIPSE, and Fundación R.Areces. Work at Centro de Astrobiología was supported by INTA, MEC, CAM and UE. CIBERehd is funded by Instituto de Salud Carlos III. S.O. was supported by a predoctoral fellowship from the Ministerio de Educacion y Ciencia.Peer reviewe
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