40 research outputs found
Distinct Transcriptome Expression of the Temporal Cortex of the Primate Microcebus murinus during Brain Aging versus Alzheimer's Disease-Like Pathology
Aging is the primary risk factor of neurodegenerative disorders such as Alzheimer's disease (AD). However, the molecular events occurring during brain aging are extremely complex and still largely unknown. For a better understanding of these age-associated modifications, animal models as close as possible to humans are needed. We thus analyzed the transcriptome of the temporal cortex of the primate Microcebus murinus using human oligonucleotide microarrays (Affymetrix). Gene expression profiles were assessed in the temporal cortex of 6 young adults, 10 healthy old animals and 2 old, “AD-like” animals that presented ß-amyloid plaques and cortical atrophy, which are pathognomonic signs of AD in humans. Gene expression data of the 14,911 genes that were detected in at least 3 samples were analyzed. By SAM (significance analysis of microarrays), we identified 47 genes that discriminated young from healthy old and “AD-like” animals. These findings were confirmed by principal component analysis (PCA). ANOVA of the expression data from the three groups identified 695 genes (including the 47 genes previously identified by SAM and PCA) with significant changes of expression in old and “AD-like” in comparison to young animals. About one third of these genes showed similar changes of expression in healthy aging and in “AD-like” animals, whereas more than two thirds showed opposite changes in these two groups in comparison to young animals. Hierarchical clustering analysis of the 695 markers indicated that each group had distinct expression profiles which characterized each group, especially the “AD-like” group. Functional categorization showed that most of the genes that were up-regulated in healthy old animals and down-regulated in “AD-like” animals belonged to metabolic pathways, particularly protein synthesis. These data suggest the existence of compensatory mechanisms during physiological brain aging that disappear in “AD-like” animals. These results open the way to new exploration of physiological and “AD-like” aging in primates
Exploring genetic resistance to infectious salmon anaemia virus in Atlantic salmon by genome-wide association and RNA sequencing
Funding The authors gratefully acknowledge funding from BBSRC (BB/R008612/1, BB/R008973/1), in addition to BBSRC Institute Strategic Programme Grants to the Roslin Institute (BB/P013759/1 and BB/P013740/1).Peer reviewedPublisher PD
A global approach to mapping the environmental risk of commercial harbours on aquatic systems
The goal of this paper is to propose a screening method for assessing the environmental risk to aquatic systems in
harbours worldwide. A semi-quantitative method is based on environmental pressures, environmental conditions
and societal response. The method is flexible enough to be applied to 15 harbours globally distributed through a
multinational test using standardised and homogenised open data that can be obtained for any port worldwide.
The method emerges as a useful approach towards the foundation of a global environmental risk atlas of harbours
that should guide the harbour sector to develop a more globally informed strategy of sustainable
development
Modeling Delamination Growth in Composite Panels subjected to Fatigue Load
One of the most critical aspects of composite structures is indeed associated to
delamination phenomenon, especially with reference to their fatigue behavior. As a matter of facts,
delaminations are strongly influenced by the fatigue induced degradation phenomena which can
lead to a significant increase of delaminated area with the number of cycles, reducing the structural
load carrying capability. In the present paper, an advanced numerical approach, very similar to the
Paris Law formulation and based on the Energy Release Rate, is presented. The proposed
formulation, in the frame of a geometrical non-linear analysis, is able to take into account the local
damage accumulation along the delamination front in order to evaluate the delamination growth
under fatigue loading conditions. In order to test the effectiveness of the proposed numerical
approach, the fatigue behavior of a delaminated panel with a central hole has been simulated and
the obtained numerical results have been compared with literature experimental results
Patients' survival in lung malignancies treated by microwave ablation: our experience on 56 patients
I paesaggi terrazzati campani
Analisi e mappatura del paesaggio terrazzato campan