Antiproliferative activity of extracts of Euphorbia tirucalli L (Euphorbiaceae) from three regions of Brazil

Purpose: To investigate Euphorbia tirucalli extract for probable geographic variations in its antiproliferative activity.Methods: The aerial parts of E. tirucalli were collected in the Brazilian states of Mato Grosso, Rio de Janeiro, Pará, Minas Gerais and Santa Catarina. The 70 % ethanol extract was obtained according to the procedure described in Brazilian Homeopathic Pharmacopeia. The antiproliferative activity of extracts, in concentrations of 62, 125, 250, and 500 μg mL-1, was tested against leukemia (HL-60), lymphoma (Daudi) and melanoma (B16F10) cell lines using methyl thiazol tetrazolium assay (MTT). Phytochemical analysis were carried out using High-performance liquid chromatography-diode array (HPLC-UV-DAD) and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI(-) FT-ICR MS) assays.Results: There was significant regional variability in the cytotoxicity of E. tirucalli extracts in a dosedependent manner. The extracts had similar activity towards leukemia cell line HL-60, decreasing cell viability to about 60 – 70 %. The extract showed the presence of ellagitannins, flavonoids, veracylglucan, and acid triterpenes as the major compounds.Conclusion: While the results support the ethnopharmacological use of E. tirucalli throughout Brazil, regional quantitative differences found in some classes of secondary metabolites may explain the variations observed in antitumor activity.Keywords: Aveloz, Cancer, Cytotoxicity, Antiproliferative, Ethnopharmacological, Traditional medicin

Identification of Alkaloids from Hippeastrum aulicum (Ker Gawl.) Herb.(Amaryllidaceae) Using CGC-MS and Ambient Ionization Mass Spectrometry (PS-MS and LS-MS)

Amaryllidaceae alkaloids are well-known isoquinolines which have demonstrated a wide range of biological activities such as antiviral, anticancer, acetylcholinesterase inhibition, antimalarial, among others. Mass spectrometry (MS) studies based on capillary gas chromatography (CGC), paper spray (PS), and leaf spray (LS) ionization were carried out for alkaloid investigation of the native Brazilian species Hippeastrum aulicum, along with nuclear magnetic resonance (NMR) techniques. Thirty-one alkaloids were identified including the new compound haemanthamine N-oxide. The results from PS- and LS-MS techniques were consistent with those observed in CGC-MS analysis. To the best of our knowledge, it is the first study combining NMR, CGC-MS and the ambient ionization-mass spectrometry (PS- and LS-MS) on Amaryllidaceae plants

Isomeric separation of cannabinoids by UPLC combined with ionic mobility mass spectrometry (TWIM-MS)-Part I

The Cannabis sativa L. plant is rich in a wide variety of cannabinoids. δ9-tetrahydrocannabinol (δ9-THC) is the main chemical compound responsible for its psychoactive effect, and it can be identified as [M+H]+ and [M-H]- ions at m/z 315 and 313, respectively, where M=C21H30O2. However, six other isomeric or isobaric forms of δ9-THC can exist, which makes its unequivocal characterization a challenge. In this work, ultra-high liquid chromatography coupled to traveling wave ion mobility mass spectrometry (UPLC-TWIM-MS) were applied to both electrospray ionization modes (ESI(±)) and used to analyze hashish, marijuana, and parts of the Cannabis Sativa L. plant (flower and leaf). The presence of a complex isomeric mixture of cannabinoids has been identified, and the mixture mainly contains δ9-THC, cannabidiol (CBN-C5 and Mw =310Da), δ9-tetrahydrocannabinolic acid A and B (δ9-THCA-C5 A/B and Mw =358Da) and their isomers. Three isomers of the ions were identified at m/z 315/313, 311, and 357 by using direct infusion ESI-TWIM-MS technique, while higher selectivity was observed in UPLC-ESI-TWIM-MS data, with the maximum isomeric separation between four and five compounds achieved when using single-ion mode (SIM) acquisition. The ions at m/z 311/309, 315/313, 345, and 357 correspond to CBN-C5, δ9-THC, cannabielsioc acid, δ9-THCA-C5 and their isomers, respectively, and they were the main species found. The calculations of collision cross sections were reported for all isomers of cannabinoids and associated with TWIM-MS results

Nutrient accumulation in fruits and grains of black pepper at different ripening stages

Funding Information: This work received funding support from Fundação de Amparo à Pesquisa e Inovação do Espírito Santo (FAPES), from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brasil - Finance code 001, from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and from Fundação para a Ciência e a Tecnologia, I.P. (FCT), Portugal, through the research units UIDB/00239/2020 (CEF), and UIDP/04035/2020 (GeoBioTec). We would like to acknowledge the Universidade Federal do Espírito Santo (UFES) for providing the facilities needed and the farmer Zenor Quinquim for field support. Publisher Copyright: © 2022, Universidade Federal de Santa Maria. All rights reserved.Nutrients accumulation in plants/fruits varies according to the crop development stage and its quantification is important to determine nutrients exportation for fertilization planning and nutrient balancing. This study determined the accumulation of nutrients in different parts of Piper nigrum ‘Bragantina’ spikes harvested at two ripening stages (still-green and red-colored spikes). Assessments in separate parts of still-green spikes (fruits and peduncles) as well as in red-colored spikes (grains, flesh and peduncles) were performed. Piper nigrum spikes were harvested, dried, and the parts were thereafter separated for chemical analysis. N and Mn were the macro and micronutrient most accumulated in the fruits of still-green spikes and in the grains of red-colored spikes, while K and B were most accumulated in the other parts of black pepper assessed, regardless of the ripening stage. The process of removing the flesh from the fruits for white pepper production led to a difference in nutrient concentration and accumulation between the fruits of still-green spikes and the grains of red-colored spikes. A significant contribution of nutrient input can be achieved by maintained flesh and peduncles in the crop area, decreasing the dependence of external fertilizers and thus contributing to a more sustainable agriculture.publishersversionpublishe

Prediction of total acid number in distillation cuts of crude oil by ESI(-) FT-ICR MS coupled with chemometric tools

FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESCompetitive adaptive reweighted sampling-partial least squares (CARS-PLS) and negative-ion mode electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI(-) FT-ICR MS) data were adopted to assess the total acid number (TAN)28918221829FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESSEM INFORMAÇÃOSEM INFORMAÇÃOSEM INFORMAÇÃOThis research was generously funded by PETROBRAS/CENPES, FAPESP, CNPq, CAPES, and FINE

Monitoring the degradation and the corrosion of naphthenic acids by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and atomic force microscopy

AbstractAlthough the term “naphthenic acids” was originally used to describe acids that contain naphthenic rings, today this term is used in a more general sense and refers to all components in the acid extractable fraction. In crude oil, naphthenic acids exist as a complex mixture of compounds with broad polydispersity with respect to both molecular weight and structure. There has been increasing interest in the naphthenic acids in crude oil because of the corrosion problems that cause during oil refining. Herein, two powerful analytical tools, negative-ion electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry, ESI(-)FT-ICR MS and atomic force microscopy (AFM), were combined to monitor the thermal degradation of naphthenic acids and their corrosion effects on AISI 1020 steel, respectively. Two different acidic crude oils (TAN=2.38 and 4.79mgKOHg−1, and total sulfur=0.7993 and 1.0220wt%) have been submitted to thermal treatment at 280, 300 and 350°C during 2, 4 and 6h, and characterized by ESI(-)-FT-ICR MS, total acid number (TAN), and total sulfur. The AISI 1020 steel was analyzed by scanning electron microscopy (SEM) and AFM. Generally, heating the crude oil at 350°C in a period of 6h, it was observed that a high efficiency (≅80%) and selectivity of thermal decarboxylation process was monitored by decay of TAN (4.79→0.44mgKOHg−1). ESI(-)-FT-ICR MS results showed that naphthenic acid species remained after the heating have DBE ranging 1–12 and carbon number from C15 to C45. AFM topographic profile evidenced that the naphthenic acid corrosion of the crude oil with TAN of 4.73mgKOHg−1 on AISI 1020 steel was profoundly altered and a marked reduction in peak to peak height values (obtained by subtracting the value of the lowest peak by the highest peak in the topographic area examined). Optical images and microphotographs confirmed the presence of irregularities, characterizing the corrosion mechanism as pitting type. The naphthenic corrosion was also evidenced in samples with low TAN value (0.44mgKOHg−1)

NBOMe : perfil de apreensões da Polícia Federal no Brasil

A Polícia Federal (PF) possui informações importantes sobre as apreensões de drogas sintéticas no país que podem ser compiladas e organizadas para descrever o panorama atual das designer drugs no Brasil sob a realidade observada pela PF. Trata-se do Sistema Criminalística, um banco de dados nacional com laudos periciais digitalizados. Neste contexto, este estudo teve como objetivo fazer um levantamento estatístico do perfil de apreensão das drogas sintéticas que contém NBOMe - grupo derivado da feniletilamina com propriedades alucinógenas - seus sais e isômeros, através da análise dos dados contidos nos relatórios periciais emitidos pela Polícia Federal no Brasil nos últimos 5 anos. As seguintes informações foram coletadas: número de laudos, local das apreensões, local de elaboração dos laudos, formas de apresentação, principais drogas apreendidas em conjunto com NBOMe e suas respectivas formas de apresentação, classificação química do NBOMe - de acordo com substituintes - quantidade de unidades apreendidas e principais imagens visualizadas nos materiais apreendidos pelos agentes federais. Foi possível traçar um panorama da situação nacional atual, sob a ótica da PF, sobre drogas sintéticas para o aprofundamento da visão, especialmente em produtos que contenham NBOMe, seus sais e isômeros. Os dados aqui apresentados se destinam a apoiar as políticas de planejamento de ações estratégicas para o controle, supressão e prevenção de uso de drogas e tráfico

Lycorine alkaloid and Crinum americanum L. (Amaryllidaceae) extracts display antifungal activity on clinically relevant candida species

Candida species are the main fungal agents causing infectious conditions in hospital patients. The development of new drugs with antifungal potential, increased efficacy, and reduced toxicity is essential to face the challenge of fungal resistance to standard treatments. The aim of this study is to evaluate the in vitro antifungal effects of two crude extracts of Crinum americanum L., a rich alkaloid fraction and lycorine alkaloid, on the Candida species. As such, we used a disk diffusion susceptibility test, determined the minimum inhibitory concentration (MIC), and characterized the components of the extracts using Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (ESI FT-ICR MS). The extracts were found to have antifungal activity against various Candida species. The chemical characterization of the extracts indicated the presence of alkaloids such as lycorine and crinine. The Amaryllidaceae family has a promising antifungal potential. Furthermore, it was found that the alkaloid lycorine directly contributes to the effects that were observed for the extracts and fraction of C. americanum

Quantification of milk adulterants (starch, H2O2, and NaClO) using colorimetric assays coupled to smartphone image analysis

In this paper, a colorimetric method for the detection of milk adulterants using smartphone image analysis is reported. This is based on the reactions to detect hydrogen peroxide, sodium hypochlorite, and starch in milk, where a color variation is observed for each substance. The image analysis was performed by using lab-made apps (PhotoMetrix®, and RedGIM®) based on partial least squares regression with the histograms of the red-green-blue images. The image histograms are automatically calculated using the smartphone camera and processed within the app. The results have shown the capability of this method to predict the concentration of the three adulterants, demonstrating the potential of the use of digital images and smartphone applications associated with chemometric tools. This method presents a fast, low-cost, and portable way to quantify adulterants in Cow milk

Annona coriacea Mart. fractions promote cell cycle arrest and inhibit autophagic flux in human cervical cancer cell lines

Plant-based compounds are an option to explore and perhaps overcome the limitations of current antitumor treatments. Annona coriacea Mart. is a plant with a broad spectrum of biological activities, but its antitumor activity is still unclear. The purpose of our study was to determine the effects of A. coriacea fractions on a panel of cervical cancer cell lines and a normal keratinocyte cell line. The antitumor effect was investigated in vitro by viability assays, cell cycle, apoptosis, migration, and invasion assays. Intracellular signaling was assessed by Western blot, and major compounds were identified by mass spectrometry. All fractions exhibited a cytotoxic effect on cisplatin-resistant cell lines, SiHa and HeLa. C3 and C5 were significantly more cytotoxic and selective than cisplatin in SiHa and Hela cells. However, in CaSki, a cisplatin-sensitive cell line, the compounds did not demonstrate higher cytotoxicity when compared with cisplatin. Alkaloids and acetogenins were the main compounds identified in the fractions. These fractions also markedly decreased cell proliferation with p21 increase and cell cycle arrest in G2/M. These effects were accompanied by an increase of H2AX phosphorylation levels and DNA damage index. In addition, fractions C3 and C5 promoted p62 accumulation and decrease of LC3II, as well as acid vesicle levels, indicating the inhibition of autophagic flow. These findings suggest that A. coriacea fractions may become effective antineoplastic drugs and highlight the autophagy inhibition properties of these fractions in sensitizing cervical cancer cells to treatment.e FINEP (MCTI/FINEP/MS/SCTIE/DECIT-01/ 2013—FP XII-BIOPLAT), Barretos Cancer Hospital, CAPES, CNPq, FAPEMIG, UFSJ. RMR is a recipient of CNPq Productivity Gran