X-Ray characterization of 3-methyl-6,8-di(2-pyridyl)-[1,2,3]triazolo[5′,1′:6,1]pyrido[2,3-d]pyrimidine

The structure of a new compound 3-methyl-6,8-di(2-pyridyl)[1,2,3]triazolo[5′,1′:6,1]pyrido[2,3- d]pyrimidine 3, formed in the reaction of 3-methyl-[1,2,3]triazolo[1,5-a]pyridine with 2-cyanopyridine, is determined by X-ray diffraction analysis.Abarca Gonzalez, Belen. [email protected] ; Ballesteros Campos, Rafael, [email protected] ; Chadlaoui, Mimoun, [email protected] ; Ramirez de Arellano Sanchez, Maria del Carmen, [email protected]

Oral saliva swab reverse transcription PCR for Covid-19 in the paediatric population

8Pág. Centro de Investigación en Sanidad Animal (CISA)To evaluate the performance of oral saliva swab (OSS) reverse transcription PCR (RT-PCR) compared with RT-PCR and antigen rapid diagnostic test (Ag-RDT) on nasopharyngeal swabs (NPS) for SARS-CoV-2 in children.The study was funded by: Project PI20/00095, from the Instituto de Salud Carlos III (Ministry of Economy, Industry and Competitiveness) and cofounded by the European Regional Development Fund, by Infanta Sofia University Hospital and Henares University Hospital Foundation for Biomedical Research and Innovation (FIIB HUIS HHEN), and by SERMAS-Fundación para la Investigación Biomédica del Hospital 12 de Octubre. EC-C is supported by the Spanish Society of Paediatrics (Asociación Española de Pediatría); Grant COVID-19 EPICO-AEP 2020. JMM is funded by SERMAS-Fundación para la Investigación Biomédica del Hospital Infanta Sofía y del Henares and by Universidad Europea de Madrid, Spain. MdlS is funded by Grant Cantera de Investigación Santander, Fundación Universidad Europea de Madrid, Spain. ED is funded by the Juan de la Cierva–Incorporación granted by the Spanish Ministry of Science and Innovation. DB-G is funded by the Spanish Ministry of Science and Innovation—Instituto de Salud Carlos III and Fondos FEDER by ’Contratos para la intensificación de la actividad investigadora en el Sistema Nacional de Salud, 2020 (INT20/00086)’.Peer reviewe

Compartir ideas. La Universidad va al Instituto: un proyecto de aprendizaje- servicio transversal de la Universidad de Barcelona

Compartir ideas. La universidad va al instituto es un proyecto de aprendizaje servicio transversal de la Universidad de Barcelona. Este representa una propuesta de aprendizaje servicio que bajo una estructura común permite la participación de estudiantes y profesorado de distintas disciplinas en un mismo proyecto. El aprendizaje servicio (ApS) es una propuesta formativa que permite el desarrollo de diferentes tipos de aprendizajes a partir de la implicación en necesidades sociales reales con la intención de transformarlas (Tapia, 2001; Martínez, 2008; Puig, 2009). En este tipo de proyectos están presentes simultáneamente la intencionalidad pedagógica y la intencionalidad solidaria. Se pueden definir como experiencias educativas solidarias protagonizadas por estudiantes, que tienen como objetivo atender a una necesidad de los destinatarios a la vez que planificar y mejorar la calidad de los aprendizajes (Tapia, 2006)

Spatiotemporal Characteristics of the Largest HIV-1 CRF02_AG Outbreak in Spain: Evidence for Onward Transmissions

Background and Aim: The circulating recombinant form 02_AG (CRF02_AG) is the predominant clade among the human immunodeficiency virus type-1 (HIV-1) non-Bs with a prevalence of 5.97% (95% Confidence Interval-CI: 5.41–6.57%) across Spain. Our aim was to estimate the levels of regional clustering for CRF02_AG and the spatiotemporal characteristics of the largest CRF02_AG subepidemic in Spain.Methods: We studied 396 CRF02_AG sequences obtained from HIV-1 diagnosed patients during 2000–2014 from 10 autonomous communities of Spain. Phylogenetic analysis was performed on the 391 CRF02_AG sequences along with all globally sampled CRF02_AG sequences (N = 3,302) as references. Phylodynamic and phylogeographic analysis was performed to the largest CRF02_AG monophyletic cluster by a Bayesian method in BEAST v1.8.0 and by reconstructing ancestral states using the criterion of parsimony in Mesquite v3.4, respectively.Results: The HIV-1 CRF02_AG prevalence differed across Spanish autonomous communities we sampled from (p < 0.001). Phylogenetic analysis revealed that 52.7% of the CRF02_AG sequences formed 56 monophyletic clusters, with a range of 2–79 sequences. The CRF02_AG regional dispersal differed across Spain (p = 0.003), as suggested by monophyletic clustering. For the largest monophyletic cluster (subepidemic) (N = 79), 49.4% of the clustered sequences originated from Madrid, while most sequences (51.9%) had been obtained from men having sex with men (MSM). Molecular clock analysis suggested that the origin (tMRCA) of the CRF02_AG subepidemic was in 2002 (median estimate; 95% Highest Posterior Density-HPD interval: 1999–2004). Additionally, we found significant clustering within the CRF02_AG subepidemic according to the ethnic origin.Conclusion: CRF02_AG has been introduced as a result of multiple introductions in Spain, following regional dispersal in several cases. We showed that CRF02_AG transmissions were mostly due to regional dispersal in Spain. The hot-spot for the largest CRF02_AG regional subepidemic in Spain was in Madrid associated with MSM transmission risk group. The existence of subepidemics suggest that several spillovers occurred from Madrid to other areas. CRF02_AG sequences from Hispanics were clustered in a separate subclade suggesting no linkage between the local and Hispanic subepidemics

A C(π-hole)⋯Cl-Zn tetrel interaction driving a metal-organic supramolecular assembly

A brominated pyrimidinyl triazolopyridine ligand (bptpy) forms a C(π-hole)⋯Cl-Zn tetrel interaction that plays a determining role in the formation of supramolecular layers through chain assembly in 18 membered metallacycle [(ZnCl2)2(μ-bptpy)2] crystals. Supramolecular chains are formed through C-H⋯X interactions. The observed interactions are supported by DFT calculations using model dimers. This journal isWe thank the Universidad de Valencia (Project UV-INV_AE18-771862) for financial support

Epitaxial films of the proton-conducting Ca-doped LaNbO4 material and a study of their charge transport properties

Ca-doped LaNbO4 (LCNO) material has shown outstanding stability in harsh environments in combination with moderate proton-conductivity in polycrystalline samples at intermediate temperatures below 800 °C under a wet atmosphere. However, in polycrystalline materials the role of grain boundaries is believed to play a predominant role in protonic conductivity, which may make more difficult the study of the intrinsic properties of the material. For that reason the present study focuses on the preparation of epitaxialfilms by pulsed laser deposition on NdGaO3 (110) single crystal substrates. Under certain deposition conditions the films grow epitaxially with a monoclinic structure (fergusonite) and b-axis orientation. A polymorph change between the monoclinic and tetragonal structure has been observed at 250 °C. The filmchargetransportproperties are analyzed under different atmospheres, dry and wet O2 and H2 as well as under deuterated conditions by using D2 and D2O in order to check for the characteristic isotopic effect of the protontransport. Although a certain proton conductivity was observed under reducing wet atmospheres unexpected large DC conductivity values (of about 5.5 S/cm for the 80 nm thin LCNO film at 800 °C in dry O2) have been observed for all films and ambient conditions.Peer Reviewe

Multi-approach analysis to assess the chromium(III) immobilization by Ochrobactrum anthropi DE2010

Ochrobactrum anthropi DE2010 is a microorganism isolated from Ebro Delta microbial mats and able to resist high doses of chromium(III) due to its capacity to tolerate, absorb and accumulate this metal. The effect of this pollutant on O. anthropi DE2010 has been studied assessing changes in viability and biomass, sorption yields and removal efficiencies. Furthermore, and for the first time, its capacity for immobilizing Cr(III) from culture media was tested by a combination of High Angle Annular Dark Field (HAADF) Scanning Transmission Electron Microscopy (STEM) imaging coupled to Energy Dispersive X-ray spectroscopy (EDX). The results showed that O. anthropi DE2010 was grown optimally at 0-2 mM Cr(III). On the other hand, from 2 to 10 mM Cr(III) microbial plate counts, growth rates, cell viability, and biomass decreased while extracellular polymeric substances (EPS) production increases. Furthermore, this bacterium had a great ability to remove Cr(III) at 10 mM (q = 950.00 mg g) immobilizing it mostly in bright polyphosphate inclusions and secondarily on the cellular surface at the EPS level. Based on these results, O. anthropi DE2010 could be considered as a potential agent for bioremediation in Cr(III) contaminated environments

Prevención de Obesógenos Ambientales

Esta guía se ha realizado dentro del marco de los siguientes proyectos de investigación: Beca de Investigación de AEPap 2021 para la investigación en Nutrición Infantil en Pediatría de Atención Primaria. GP/EFSA/ENCO/2018/03 (European Food Safety Authority). PE-0250-2019 (Consejería de Salud de la Junta de Andalucía). P18-RT-4247 (Consejería de Economía, Conocimiento, Empresas y Universidad de la Junta de Andalucía). 30.BF.88.08.01 (Fundación MAPFRE).En esta guía nos gustaría introducir de manera sencilla, clara y práctica, teorías y técnicas que ayuden a combatir la obesidad infantil, un problema que está cada vez más presente en nuestra sociedad y que puede afectar indiscriminadamente a todas las etapas de la vida. Es un recopilatorio de los aspectos más relevantes en nuestra cotidianidad, realizado con términos, conceptos y ejemplos claros que permiten la comprensión de toda la unidad familiar. Nos adentramos en el novedoso mundo de los obesógenos ambientales y comprendemos el porqué y el cómo pueden dañar nuestra salud y la de nuestros pequeños. A lo largo de toda la guía, se han introducido sugerencias y propuestas que pueden ayudar al lector a incorporar estos nuevos u olvidados conocimientos y hábitos.Asociación Española de Pediatría de Atención Primaria (AEPap)Asociación Andaluza de Pediatría de Atención Primaria (AndAPap)Pediatría de Atención Primaria AEPap 2021European Food Safety Authority GP/EFSA/ENCO/2018/03Junta de Andalucía PE-0250-2019, P18-RT-4247Fundación MAPFRE 30.BF.88.08.0

Kinetic properties of the redox switch/redox coupling mechanism as determined in primary cultures of cortical neurons and astrocytes from rat brain

We investigate the mechanisms underlying the redox switch/redox coupling hypothesis by characterizing the competitive consumption of glucose or lactate and the kinetics of pyruvate production in primary cultures of cortical neurons and astrocytes from rat brain. Glucose consumption was determined in neuronal cultures incubated in Krebs ringer bicarbonate buffer (KRB) containing 0.25–5 mM glucose, in the presence and absence of 5 mM lactate as an alternative substrate. Lactate consumption was measured in neuronal cultures incubated with 1–15 mM lactate, in the presence and absence of 1 mM glucose. In both cases, the alternative substrate increased the Km (mM) values for glucose consumption (from 2.2 ± 0.2 to 3.6 ± 0.1) or lactate consumption (from 7.8 ± 0.1 to 8.5 ± 0.1) without significant changes on the corresponding Vmax. This is consistent with a competitive inhibition between the simultaneous consumption of glucose and lactate. When cultures of neurons or astrocytes were incubated with increasing lactate concentrations 1–20 mM, pyruvate production was observed with Km (mM) and Vmax (nmol/mg/h) values of 1.0 ± 0.1 and 109 ± 4 in neurons, or 0.28 ± 0.1 and 342 ± 54 in astrocytes. Thus, astrocytes or neurons are able to return to the incubation medium as pyruvate, a significant part of the lactate consumed. Present results support the reversible exchange of reducing equivalents between neurons and astrocytes in the form of lactate or pyruvate. Monocarboxylate exchange is envisioned to operate under near equilibrium, with the transcellular flux directed thermodynamically toward the more oxidized intracellular redox environment.B.G.R. was a postdoctoral fellow of C.S.I.C. and I.R.V. received an Erasmus fellowship from the University of Coimbra. M.M.C.A.C. was on a sabbatical visit from the University of Coimbra (Portugal). T.B.R. and L.L.F. received pre-doctoral fellowships from the Portuguese FCT. M.L.G.M. holds a tenure track ‘‘Ramón y Cajal’’ contract from CSIC. S.C. receives a grant from Institute of Health Carlos III, as does M.L.G.M. Portuguese Fundaçäo para a Ciência e a Tecnologia provides funding for T.B.R. and L.L.F. Contract grant sponsor: Spanish Ministry of Education and Science; Contract grant numbers: SAF 2001-2245, SAF 2004-03197; Contract grant sponsor: Institute of Health Carlos III; Contract grant numbers: PI051530, PI051845; Contract grant sponsor: Portuguese Fundaçäo para a Ciência e a Tecnologia; Contract grant numbers: SFRH/BD/5407/2001, PRAXIS XXI/BD/21532/99.Peer Reviewe