Orbital and physical parameters of eclipsing binaries from the All-Sky Automated Survey catalogue - VI. AK Fornacis - a rare, bright K-type eclipsing binary

We present the results of the combined photometric and spectroscopic analysis of a bright (V=9.14), nearby (d=31 pc), late-type detached eclipsing binary AK Fornacis. This P=3.981 d system has not been previously recognised as a double-lined spectroscopic binary, and this is the first full physical model of this unique target. With the FEROS, CORALIE and HARPS spectrographs we collected a number of high-resolution spectra in order to calculate radial velocities of both components of the binary. Measurements were done with our own disentangling procedure and the TODCOR technique, and were later combined with the photometry from the ASAS and SuperWASP archives. We also performed an atmospheric analysis of the component spectra with the Spectroscopy Made Easy (SME) package. Our analysis shows that AK For consists of two active, cool dwarfs having masses of $M_1=0.6958 \pm 0.0010$ and $M_2=0.6355 \pm 0.0007$ M$_\odot$ and radii of $R_1=0.687 \pm 0.020$ and $R_2=0.609 \pm 0.016$ R$_\odot$, slightly less metal abundant than the Sun. Parameters of both components are well reproduced by the models. AK For is the brightest system among the known eclipsing binaries with K or M type stars. Its orbital period is one of the longest and rotational velocities one of the lowest, which allows us to obtain very precise radial velocity measurements. The precision in physical parameters we obtained places AK For among the binaries with the best mass measurements in the literature. It also fills the gap in our knowledge of stars in the range of 0.5-0.8 M$_\odot$, and between short and long-period systems. All this makes AK For a unique benchmark for understanding the properties of low-mass stars.Comment: 9 pages, 11 figures, 3 tables, accpeted for publication in A&

Analyses of the vrl gene cluster in Desulfococcus multivorans: Homologous to the virulence-associated locus of the ovine footrot pathogen Dichelobacter nodosus strain A198

Major parts of the virulence-associated vrl locus known from the gammaproteobacterium Dichelobacter nodosus, the causative agent of ovine footrot, were analyzed in the genome of the sulfate-reducing deltaproteobacterium Desulfococcus multivorans. In the genome of D. multivorans 13 of the 19 vrl genes described for D. nodosus are present and highly conserved with respect to gene sequence and order. The vrl locus and its flanking regions suggest a bacteriophage-mediated transfer into the genome of D. multivorans. Comparative analysis of the deduced Vrl proteins reveals a wide distribution of parts of the virulence-associated vrl locus in distantly related bacteria. Horizontal transfer is suggested as driving mechanism for the circulation of the vrl genes in bacteria. Except for the vrlBMN genes D. multivorans and Desulfovibrio desulfuricans G20 together contain all vrl genes displaying a high degree of similarity. For D. multivorans it could be shown that guanine plus cytosine (GC) content, GC skew, di-, tri- or tetranucleotide distribution did not differ between the vrl locus and its flanking sequences. This could be a hint that the vrl locus originated from a related organism or at least a genome with similar characteristics. The conspicuous high degree of conservation of the analyzed vrl genes may result from a recent transfer event or reflect a function of the vrl genes, which is still unknown and not necessarily disease associated. The latter is supported by the evidence for expression of the vrl genes in D. multivorans, which has not been described as pathogen or to be associated to any disease pattern before

Ground-based near-IR observations of the secondary eclipse of CoRoT-2b

We present the results of a ground-based search for the secondary eclipse of the 3.3 Mjup transiting planet CoRoT-2b. We performed near infrared photometry using the LIRIS instrument on the 4.2 m William Herschel Telescope, in the H and K_s filters. We monitored the star around two expected secondary eclipses in two nights under very good observing conditions. For the depth of the secondary eclipse we find in H-band a 3 sigma upper limit of 0.17%, whereas we detected a tentative eclipse with a depth of 0.16+-0.09% in the K_s-band. These depths can be translated into brightness temperatures of T_H<2250 K and T_{K_s} = 1890(+260-350) K, which indicate an inefficient re-distribution of the incident stellar flux from the planet's dayside to its nightside. Our results are in agreement with the CoRoT optical measurement (Alonso et al. 09) and with Spitzer 4.5 and 8 micron results (Gillon et al. 09c).Comment: Astronomical Journal, accepte

Molecular genetic and crystal structural analysis of 1-(4-hydroxyphenyl)-ethanol dehydrogenase from 'Aromatoleum aromaticum' EbN1.

he dehydrogenation of 1-(4-hydroxyphenyl)-ethanol to 4-hydroxyacetophenone represents the second reaction step during anaerobic degradation of p-ethylphenol in the denitrifying bacterium ‘Aromatoleum aromaticum' EbN1. Previous proteogenomic studies identified two different proteins (ChnA and EbA309) as possible candidates for catalyzing this reaction [Wöhlbrand et al: J Bacteriol 2008;190:5699-5709]. Physiological-molecular characterization of newly generated unmarked in-frame deletion and complementation mutants allowed defining ChnA (renamed here as Hped) as the enzyme responsible for 1-(4-hydroxyphenyl)-ethanol oxidation. Hped [1-(4-hydroxyphenyl)-ethanol dehydrogenase] belongs to the ‘classical' family within the short-chain alcohol dehydrogenase/reductase (SDR) superfamily. Hped was overproduced in Escherichia coli, purified and crystallized. The X-ray structures of the apo- and NAD+-soaked form were resolved at 1.5 and 1.1 Å, respectively, and revealed Hped as a typical homotetrameric SDR. Modeling of the substrate 4-hydroxyacetophenone (reductive direction of Hped) into the active site revealed the structural determinants of the strict (R)-specificity of Hped (Phe187), contrasting the (S)-specificity of previously reported 1-phenylethanol dehydrogenase (Ped; Tyr93) from strain EbN1 [Höffken et al: Biochemistry 2006;45:82-93]

Carbohydrate Catabolism in Phaeobacter inhibens DSM 17395, a Member of the Marine Roseobacter Clade

Since genome analysis did not allow unambiguous reconstruction of transport, catabolism, and substrate-specific regulation for several important carbohydrates in Phaeobacter inhibens DSM 17395, proteomic and metabolomic analyses of N-acetylglucosamine-, mannitol-, sucrose-, glucose-, and xylose-grown cells were carried out to close this knowledge gap. These carbohydrates can pass through the outer membrane via porins identified in the outer membrane fraction. For transport across the cytoplasmic membrane, carbohydrate-specific ABC transport systems were identified. Their coding genes mostly colocalize with the respective "catabolic" and "regulatory" genes. The degradation of N-acetylglucosamine proceeds via N-acetylglucosamine-6-phosphate and glucosamine-6-phosphate directly to fructose-6-phosphate; two of the three enzymes involved were newly predicted and identified. Mannitol is catabolized via fructose, sucrose via fructose and glucose, glucose via glucose-6-phosphate, and xylose via xylulose-5-phosphate. Of the 30 proteins predicted to be involved in uptake, regulation, and degradation, 28 were identified by proteomics and 19 were assigned to their respective functions for the first time. The peripheral degradation pathways feed into the Entner-Doudoroff (ED) pathway, which is connected to the lower branch of the Embden-Meyerhof-Parnas (EMP) pathway. The enzyme constituents of these pathways displayed higher abundances in P. inhibens DSM 17395 cells grown with any of the five carbohydrates tested than in succinate-grown cells. Conversely, gluconeogenesis is turned on during succinate utilization. While tricarboxylic acid (TCA) cycle proteins remained mainly unchanged, the abundance profiles of their metabolites reflected the differing growth rates achieved with the different substrates tested. Homologs of the 74 genes involved in the reconstructed catabolic pathways and central metabolism are present in various Roseobacter clade members

Characterising a Si(Li) detector element for the SIXA X-ray spectrometer

The detection efficiency and response function of a Si(Li) detector element for the SIXA spectrometer have been determined in the 500 eV to 5 keV energy range using synchrotron radiation emitted at a bending magnet of the electron storage ring BESSY, which is a primary radiation standard. The agreement between the measured spectrum and the model calculation is better than 2%. PACS: 95.55.Ka; 07.85.Nc; 29.40.Wk; 85.30.De Keywords: Si(Li) detectors, X-ray spectrometers, detector calibration, X-ray response, spectral lineshapeComment: 11 pages, 11 PostScript figures, uses elsart.sty, submitted to Nucl. Instrum. Meth.

A cool starspot or a second transiting planet in the TrES-1 system?

We investigate the origin of a flux increase found during a transit of TrES-1, observed with the HST. This feature in the HST light curve cannot be attributed to noise and is supposedly a dark area on the stellar surface of the host star eclipsed by TrES-1 during its transit. We investigate the likeliness of two possible hypothesis for its origin: A starspot or a second transiting planet. We made use of several transit observations of TrES-1 from space with the HST and from ground with the IAC-80 telescope. On the basis of these observations we did a statistical study of flux variations in each of the observed events, to investigate if similar flux increases are present in other parts of the data set. The HST observation presents a single clear flux rise during a transit whereas the ground observations led to the detection of two such events but with low significance. In the case of having observed a starspot in the HST data, assuming a central impact between the spot and TrES-1, we would obtain a lower limit for the spot radius of 42000 km. For this radius the spot temperature would be 4690 K, 560 K lower then the stellar surface of 5250 K. For a putative second transiting planet we can set a lower limit for its radius at 0.37 R$_J$ and for periods of less than 10.5 days, we can set an upper limit at 0.72 R$_J$. Assuming a conventional interpretation, then this HST observation constitutes the detection of a starspot. Alternatively, this flux rise might also be caused by an additional transiting planet. The true nature of the origin can be revealed if a wavelength dependency of the flux rise can be shown or discarded with a higher certainty. Additionally, the presence of a second planet can also be detected by radial velocity measurements.Comment: 8 pages, 6 figures, accepted for publication in A&

Limits to the planet candidate GJ 436c

We report on H-band, ground-based observations of a transit of the hot Neptune GJ 436b. Once combined to achieve sampling equivalent to archived observations taken with Spitzer, our measurements reach comparable precision levels. We analyze both sets of observations in a consistent way, and measure the rate of orbital inclination change to be of 0.02+/-0.04 degrees in the time span between the two observations (253.8 d, corresponding to 0.03+/-0.05 degrees/yr if extrapolated). This rate allows us to put limits on the relative inclination between the two planets by performing simulations of planetary systems, including a second planet, GJ 436c, whose presence has been recently suggested (Ribas et al. 2008). The allowed inclinations for a 5 M_E super-Earth GJ 436c in a 5.2 d orbit are within ~7 degrees of the one of GJ 436b; for larger differences the observed inclination change can be reproduced only during short sections (<50%) of the orbital evolution of the system. The measured times of three transit centers of the system do not show any departure from linear ephemeris, a result that is only reproduced in <1% of the simulated orbits. Put together, these results argue against the proposed planet candidate GJ 436c.Comment: Replaced with accepted version. Minor language corrections. 4 pages, 4 figures, to appear in A&A Letter