Puppy love in the time of Corona: dog ownership protects against loneliness for those living alone during the COVID-19 lockdown

Background: The COVID-19 pandemic has resulted in many countries enforcing a ‘lockdown’, whereby people are instructed to stay at home. Aim: The aim of this study was to capture the experience of such a lockdown in Australians living alone, with and without a dog or a cat. Method: Three hundred and eighty-four participants completed an online survey evaluating their levels of loneliness, mindfulness and mood. For participants who owned a dog or a cat, a measure of dog/cat interactions was also administered as well as two open-ended questions about how being a pet owner affected their experience of COVID-19 and how COVID-19 affected their pet. Results: Contrary to expectations, cat owners were found to be less mindful than non-owners and pet interactions did not account for levels of loneliness or levels of mindfulness. In line with our expectations, however, stress and depression positively predicted loneliness, while mindfulness and being a dog owner were protective against it. Insights from qualitative responses suggest that this might be due to the fact that dogs encourage a routine which involves getting out of the house and walking, which itself offers opportunities to socialise with other people doing the same thing. Conclusion: These findings add to the emerging literature on mental well-being during a lockdown and the unique role that pets play in their owners’ experiences

Paws for thought: the importance of dogs in a seniors social intervention

As Australia faces an aging population with an unprecedented life expectancy, it is the community’s obligation to ensure seniors are offered resources to support their well-being. Studies investigating loneliness in aged-care facilities attest to the therapeutic properties of dogs for residents’ sense of well-being. Consequently, this study aimed to ascertain the effectiveness of a community-based dog lover’s initiative for the self-management of well-being among senior citizens. Our qualitative research investigated whether community gatherings including dogs would produce greater feelings of subjective well-being among senior citizens compared to community gatherings without dogs. A conventional content analysis provided support for the supposition that dogs address some of the unmet needs of senior citizens by increasing well-being. The multitude of benefits provided by this human-animal friendship undeniably merits inclusion as a community initiative aimed at improving both the well-being of our senior citizens and the health of the community at large. It is anticipated that these findings will inspire a new field within social gerontology dedicated to promoting the human-animal bond via community initiatives

The perceptions and experiences of Australian pet owners as a result of easing COVID-19 restrictions: Insights from an online survey

The majority of Australian dog and cat owners living alone during the first COVID‑19 lockdown endorsed the idea that their pet made the isolation easier, reduced feelings of loneliness, and provided companionship. More companionship/a>ention was also the most highly endorsed way pet owners perceived that the lockdown affected their pets. With the advent of the removal of the first lockdown restrictions and an a>empt to return to a ‘COVID normal’ lifestyle, the aim of the current study was to elucidate how pet ownership affected the experience of the easing of restrictions in Australia, and how returning to a state of ‘normalcy’ might have impacted pets. A total of 208 pet owners from Australian states and territories completed an online questionnaire. Participants included 101 dog owners and 107 cat owners. Results revealed that despite official easing of restrictions, ongoing concern of contracting COVID‑19 played a role in participant tendency to continue a reduced level of socialising and going outside the house. As a result, there was minimal perceived behavioural and/or emotional impact on most pets. However, for those who were returning to work and/or a life more outside the home, there were reports that both owners and their pets experienced separation anxiety. Participants also reported that they benefited from interaction with pets while working from home, and therefore implementing more pet‑friendly policies in workplaces should be considered

A qualitative insight into the removal of the Australian COVID-19 lockdowns on dogs and cats, and their owners

The majority of Australian dog and cat owners living alone during the first COVID-19 lockdown endorsed the idea that their pet made the isolation easier, reduced feelings of loneliness, and provided companionship. More companionship/attention was also the most highly endorsed way pet owners perceived that the lockdown affected their pets. With the advent of the removal of the first lockdown restrictions and an attempt to return to a ‘COVID normal’ lifestyle, the aim of the current study was to elucidate how pet ownership affected the experience of the easing of restrictions in Australia, and how returning to a state of ‘normalcy’ might have impacted pets. A total of 208 pet owners from Australian states and territories completed an online questionnaire. Participants included 101 dog owners and 107 cat owners. Results revealed that despite official easing of restrictions, ongoing concern of contracting COVID-19 played a role in participant tendency to continue a reduced level of socialising and going outside the house. As a result, there was minimal perceived behavioural and/or emotional impact on most pets. However, for those who were returning to work and/or a life more outside the home, there were reports that both owners and their pets experienced separation anxiety. Participants also reported that they benefited from interaction with pets while working from home, and therefore implementing more pet-friendly policies in workplaces should be considered

Paws for Thought: The Importance of Dogs in a Seniors Social Intervention

As Australia faces an aging population with an unprecedented life expectancy, it is the community’s obligation to ensure seniors are offered resources to support their well-being. Studies investigating loneliness in aged-care facilities attest to the therapeutic properties of dogs for residents’ sense of well-being. Consequently, this study aimed to ascertain the effectiveness of a community-based dog lover’s initiative for the self-management of well-being among senior citizens. Our qualitative research investigated whether community gatherings including dogs would produce greater feelings of subjective well-being among senior citizens compared to community gatherings without dogs. A conventional content analysis provided support for the supposition that dogs address some of the unmet needs of senior citizens by increasing well-being. The multitude of benefits provided by this human-animal friendship undeniably merits inclusion as a community initiative aimed at improving both the well-being of our senior citizens and the health of the community at large. It is anticipated that these findings will inspire a new field within social gerontology dedicated to promoting the human-animal bond via community initiatives

Editorial: Oxytocin and Social Behaviour in Dogs and Other (Self-)Domesticated Species: Methodological Caveats and Promising Perspectives

[Extract] Over the past decade the oxytocin system has become a focus of attention for researchers from various fields studying mechanisms underlying different forms of social behavior. Some have even suggested that it is the neurohormone, oxytocin, that has had the most permissive role in the evolution of the human nervous system (Carter, 2014), implying that Homo sapiens could not have evolved without it, as the success of this species highly depends on social behavior and cognition. Not surprisingly research into model systems of human social behavior has followed this trend including several discoveries on the relatedness of numerous forms of domestic species' social behavior and their respective oxytocin systems. This is particularly interesting as domestic species are known to have adapted to the human social environment in evolutionary terms, however the proximal and distal mechanisms underlying behavioral parallels between humans and domestic animals still remain largely unexplored

Working smarter not harder: oxytocin increases domestic dogs’ (Canis familiaris) accuracy, but not attempts, on an object choice task

The neuropeptide oxytocin (OT) has been shown to enhance dogs’ ability to perform an object choice task (OCT) involving the use of human pointing cues, when delivered intranasally. This study aimed at further investigating whether OT enhances task performance by increasing choices made, or by increasing correctness of choices made, and to compare these treatment effects to dog appeasing pheromone (DAP), known to balance emotional activation in dogs. Hence, we compared OCT performance between three groups of dogs: (i) dogs administered OT and a sham collar, (ii) dogs administered a saline placebo and a DAP collar, and (iii) control dogs administered a saline placebo and a sham collar. All three groups consisted of a combination of male and female pet dogs and assistance-dogs-in-training currently living with a volunteer carer. The study also evaluated the effect of intranasal OT and/or DAP on plasma levels of OT, and prolactin; which has previously been linked with anxiety in dogs. The dogs’ emotional state was measured using the Emotional Disorders Evaluation in Dogs (EDED) scale. The owners’/carers’ degree of anxious- and avoidant-style attachment to their dogs was accessed using the Pet Attachment Questionnaire (PAQ). Interesting descriptive data appeared for both treatment groups. Particularly, in OT group, we obtained significant results demonstrating that intranasal OT enhances OCT performance in dogs compared to control, by increasing the percentage of correct choices, but not the number of choices, made. Results also support that the mode of action of intranasal OT is via direct access to the brain and not via the blood, since no elevation of plasma OT (or prolactin) levels were observed after intranasal administration in this study. Similarly, DAP application did not significantly alter OT or prolactin peripheral concentrations. Several differences were observed between fostered and pet dogs, namely: fostered dogs demonstrated higher levels of serum prolactin, made more choices on the OCT compared to pet dogs but were not more likely to be correct, and were fostered by carers with higher avoidant attachment scores than pet dog owners. These findings implicate consideration of potential carer and training consequences for assistance dogs

Generation of a transgenic zebrafish model of Tauopathy using a novel promoter element derived from the zebrafish eno2 gene

The aim of this study was to isolate cis-acting regulatory elements for the generation of transgenic zebrafish models of neurodegeneration. Zebrafish enolase-2 (eno2) showed neuronal expression increasing from 24 to 72 h post-fertilization (hpf) and persisting through adulthood. A 12 kb eno2 genomic fragment, extending from 8 kb upstream of exon 1 to exon 2, encompassing intron 1, was sufficient to drive neuronal reporter gene expression in vivo over a similar time course. Five independent lines of stable Tg(eno2 : GFP) zebrafish expressed GFP widely in neurons, including populations with relevance to neurodegeneration, such as cholinergic neurons, dopaminergic neurons and cerebellar Purkinje cells. We replaced the exon 2-GFP fusion gene with a cDNA encoding the 4-repeat isoform of the human microtubule-associated protein Tau. The first intron of eno2 was spliced with high fidelity and efficiency from the chimeric eno2-Tau transcript. Tau was expressed at ∼8-fold higher levels in Tg(eno2 : Tau) zebrafish brain than normal human brain, and localized to axons, neuropil and ectopic neuronal somatic accumulations resembling neurofibrillary tangles. The 12 kb eno2 promoter drives high-level transgene expression in differentiated neurons throughout the CNS of stable transgenic zebrafish. This regulatory element will be useful for the construction of transgenic zebrafish models of neurodegeneration

Targeting CD34(+) cells of the inflamed synovial endothelium by guided nanoparticles for the treatment of rheumatoid arthritis

Despite the advances in the treatment of rheumatoid arthritis (RA) achieved in the last few years, several patients are diagnosed late, do not respond to or have to stop therapy because of inefficacy and/or toxicity, leaving still a huge unmet need. Tissue-specific strategies have the potential to address some of these issues. The aim of the study is the development of a safe nanotechnology approach for tissue-specific delivery of drugs and diagnostic probes. CD34 + endothelial precursors were addressed in inflamed synovium using targeted biodegradable nanoparticles (tBNPs). These nanostructures were made of poly-lactic acid, poly-caprolactone, and PEG and then coated with a synovial homing peptide. Immunofluorescence analysis clearly demonstrated their capacity to selectively address CD34 + endothelial cells in synovial tissue obtained from human, mouse, and rat. Biodistribution studies in two different animal models of rheumatoid arthritis (antigen-induced arthritis/AIA and collagen-induced arthritis/CIA) confirmed the selective accumulation in inflamed joints but also evidenced the capacity of tBNP to detect early phases of the disease and the preferential liver elimination. The therapeutic effect of methotrexate (MTX)-loaded tBNPs were studied in comparison with conventional MTX doses. MTX-loaded tBNPs prevented and treated CIA and AIA at a lower dose and reduced administration frequency than MTX. Moreover, MTX-loaded tBNP showed a novel mechanism of action, in which the particles target and kill CD34 + endothelial progenitors, preventing neo-angiogenesis and, consequently, synovial inflammation. tBNPs represent a stable and safe platform to develop highly-sensitive imaging and therapeutic approaches in RA targeting specifically synovial neo-angiogenesis to reduce local inflammation

Genetic effects on gene expression across human tissues

Characterization of the molecular function of the human genome and its variation across individuals is essential for identifying the cellular mechanisms that underlie human genetic traits and diseases. The Genotype-Tissue Expression (GTEx) project aims to characterize variation in gene expression levels across individuals and diverse tissues of the human body, many of which are not easily accessible. Here we describe genetic effects on gene expression levels across 44 human tissues. We find that local genetic variation affects gene expression levels for the majority of genes, and we further identify inter-chromosomal genetic effects for 93 genes and 112 loci. On the basis of the identified genetic effects, we characterize patterns of tissue specificity, compare local and distal effects, and evaluate the functional properties of the genetic effects. We also demonstrate that multi-tissue, multi-individual data can be used to identify genes and pathways affected by human disease-associated variation, enabling a mechanistic interpretation of gene regulation and the genetic basis of diseas