Stolpersteine beim Einstieg in die neue Führungsrolle

Für angehende Führungskräfte ist der Wechsel in eine Chefposition mit vielen Unsicherheiten und Fragen behaftet: Wie ist die Kultur der Organisation? Wie "tickt" mein Vorgesetzter? Was erwarten meine Mitarbeitenden? Führungserfolg ist nicht planbar, aber mit geeigneten Massnahmen kann die Erfolgswahrscheinlichkeit erhöht werden

Kleinteiligkeit im Ackerbau ein neues Vertragsnaturschutzprogramm für den Ökologischen Landbau

A proposal for a new contract nature protection scheme was tested on selected arable fields of organic farms in northern Germany. Management specifications included small scale division of large fields combined with specifications for crop distribution as well as for proportion of flower strips. Results of collateral bird registrations proved positive effects of the scheme on diversity and abundance of (threatened) breeding and roosting bird species. Due to the results, the scheme was included in the current (regional) rural development program Schleswig-Holstein

Ermittlung des „Biodiversitätswerts“ landwirtschaftlicher Betriebe im Rahmen einzelbetrieblicher Naturschutzberatungen

The feasibility of a new accelerated procedure to determine the “value of biodiversity” of agricultural holdings was tested on 80 farms in in the federal state of Schleswig-Holstein. The results of the practical application show, that the indicator system is a suitable method to be applied in the context of consultations on nature conservation on farms. Farm advisory also implied grants for conservation schemes. Implementation of measures on organic as well as on conventional farms was notably high

Co-expression of LKB1, MO25α and STRADα in bacteria yield the functional and active heterotrimeric complex

The tumour suppressor LKB1 plays a critical role in cell proliferation, polarity and energy metabolism. LKB1 is a Ser/Thr protein kinase that is associated with STRAD and MO25 invivo. Here, we describe the individual expression of the three components of the LKB1 complex using monocistronic vectors and their co-expression using tricistronic vectors that were constructed from monocistronic vectors using a fully modular cloning approach. The data show that among the three individually expressed components of the LKB1 complex, only MO25α can be expressed in soluble form, whereas the other two, LKB1 and STRADα are found almost exclusively in inclusion bodies. However, using the tricistronic vector system, functional LKB1-MO25α-STRADα complex was expressed and purified from soluble extracts by sequential immobilized-metal affinity and heparin chromatography, as shown by Western blotting using specific antibodies. In size exclusion chromatography, MO25α and STRADα exactly co-elute with LKB1 with an apparent molecular weight of the heterotrimeric complex of 160kDa. The specific activity in the peak fraction of the size exclusion chromatography was 250U/mg at approximately 25% purity. As shown by autoradiography, LKB1 and STRADα, both strongly autophosphorylate in vitro. Moreover, recombinant LKB1 complex activates AMPK by phosphorylation of the α-subunit at the Thr-172 site as shown (i) by Western blotting using phospho-specific antibodies after LKB1-dependent phosphorylation, (ii) by LKB1-dependent incorporation of radioactive phosphate into the α-subunit of kinase dead AMPK heterotrimer, and (iii) by activity determination of AMPK. Functional mammalian LKB1 complex is constitutively active, and when enriched from bacteria should prove to be a valuable tool for studying its molecular function and regulatio

Amplification of ultrafast pulses in an extended Mamyshev regenerator

We present the characteristics of a high-energy ultrafast Yb-fiber laser system, based on a Mamyshev oscillator and a subsequently arranged fiber amplifier stage. The Mamyshev oscillator emits pulses at a repetition rate of 11 MHz and pulse energies of 31 nJ. These pulses are spectrally filtered and amplified in a Yb-doped fiber up to 1 μJ pulse energy which could be temporally dechirped to less than 50 fs autocorrelation duration. We discuss the scaling as well as limiting options related to pulse energies and duration. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only

A closed circuit electro-hydraulic actuator with energy recuperation capability

The recent electrification trend in the off-road market has incentivized research towards the proposal of compact, cost-effective and energy-efficient solutions for hydraulic actuators. As a result, increased attention has been given to electro-hydraulic actuator (EHA) architectures. The paper offers a study performed on a novel closed-circuit EHA architecture with the goal to maximize the overall system efficiency while meeting or exceeding traditional off-road applications performance, thereby enabling further electrification of off-road applications. Both numerical and experimental approaches are utilized to validate the functionality of the proposed EHA circuital configuration in four quadrants. Moreover, the actuator functionality at both high and low velocities are considered, which has never been explored in the past due to the limitations on the hydraulic machine driving speed. The good match between the experimental data and the simulation results confirms the potential of the simulation model for sizing such EHA architecture for different actuator sizes, duty cycles, and performance levels

Language concepts and design patterns

Programming languages aim at the construction of simple but expressive programs. To achieve this, plenty of language concepts have arisen over time. Design patterns aim at the solution of common design problems. To achieve this, plenty of approved design concepts have been collected. We claim that language concepts and design patterns are essentially the same. Indeed, a language may offer a design pattern as a language concept; we call such patterns "language patterns". A design pattern can be implemented in terms of other design or language patterns. Since a concrete programming language only supports a subset of language patterns, every other pattern must be expressed in terms of this subset. We call such an implementation a "workaround". The specification of a workaround imposes proof obligations: it must be shown that a workaround simulates the pattern. Once proved correct, we can collect patterns and their workarounds in a trustworthy catalogue. This helps software developers to correctly apply patterns in any language and helps the language designer to decide which patterns to put into the language core. We demonstrate this pattern integration process with well-known design patterns and concepts of object-oriented languages. Additionally, we list important language patterns together with their workarounds

Querying Semantic Web Resources Using TRIPLE Views

Resources on the Semantic Web are described by metadata based on some formal or informal ontology. It is a common situation that casual users are not familiar with a domain ontology in detail. This makes it difficult for such users (or their user tools) to formulate queries to find the relevant resources. Users consider the resources in their specific context, so the most straightforward solution is to formulate queries in an ontology that corresponds to a user-specific view. We present an approach based on multiple views expressed in ontologies simpler than the domain ontology. This allows users to query heterogeneous data repositories in terms of multiple, relatively simple, view ontologies. Ontology developers can define such view ontologies and the corresponding mapping rules. These ontologies are represented in Semantic Web ontology languages such as RDFS, DAML+OIL, or OWL. We present our approach with examples from the e-learning domain using the Semantic Web query and transformation language TRIPLE

Root canal pre-treatment and adhesive system affect bond strength durability of fiber posts ex vivo

Objectives: To investigate the effect of different pre-treatments on the long-term bond strength of fiberglass posts luted either with dual-curing self-etch adhesives and core build-up composites or with a self-adhesive resin (SAR) cement. Materials and methods: In total, 180 human root-filled teeth received post-space preparations and three different dentin pre-treatments (PTs): PT1, ethanol (99%); PT2, ethanol-tertiary-butanol-water-solution (AH Plus Cleaner, Dentsply Sirona; York, USA); and PT3, distilled water (control). Five luting systems were used: FU, Futurabond U (Voco; Cuxhaven, Germany); CL, Clearfil DC Bond (Kuraray Noritake; Okayama, Japan); GR, Gradia Core SE Bond (GC Europe NV; Leuven, Belgium); LU, LuxaBond Universal (DMG; Hamburg, Germany); and RX, RelyX Unicem 2 (3M; Minnesota, USA). Roots were cut into six slices (1 mm thick). From each root canal region, three slices were submitted to immediate and three to post-storage push-out testing. The latter were subjected to thermocycling (5–55°C, 6.000 cycles) and stored for six months in saline solution (0.9%, 37°C). Data were analysed using repeated measures ANOVA and chi-square tests (MV±SD). Results: Bond strength was significantly affected by material (p<0.0005), pre-treatment (p=0.016), and storage (p<0.0005; repeated-measures ANOVA). LU (18.8±8.1MPa) revealed significantly higher bond strength than RX (16.08±6.4MPa), GR (15.1±4.6MPa), CL (13.95±5.2MPa), and FU (13.7±6.3MPa). PT1 (16.5±6.9MPa) revealed significantly higher bond strength than PT3 (14.5±5.7MPa). Conclusions: A universal adhesive in self-etch mode combined with a core build-up material revealed higher bond strength than a SAR cement, both interacted positively with Ethanol pre-treatment. Clinical relevance statement: Ethanol (99%) rinsing can be recommended as part of post and core pre-treatment for the investigated luting systems